Endogenous hydrogen sulfide regulates oxidant/antioxidant balance in rat with chronic obstructive pulmonary disease

AIM: To investigate the role of hydrogen sulfide (H₂S) in the protection against oxidative stress in rats with chronic obstructive pulmonary disease (COPD).METHODS: The rat model of COPD was established by cigarette smoking (CS) combined with lipopolysaccharide (LPS) instillation. Thirty-two healthy male Sprague-Dawley rats were randomly divided into 4 groups: control group, CS+LPS group, CS+LPS+NaHS (H₂S donor) group and CS+LPS+PPG (DL-propargylglycine, an inhibitor of cystathionine-γ-lyase) group. After 30 days, the lung functions of the rats were measured, the histological changes of lungs were observed under light microscope and the pathological scores were calculated. The H₂S level in plasma and the protein expression of cystathionine-γ-lyase (CSE) in the lung tissues were measured. The content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and catalase (CAT) were detected to reflect oxidative stress.RESULTS: Compared with control group, the peak expiratory flow (PEF) decreased by 24% and intra-pressure (IP) increased by 66% in CS+LPS group. The pathological scores of the lung tissues also increased. Compared with CS+LPS group, no change in the lung function was observed after given NaHS or PPG, but the pathological scores decreased in CS+ LPS+ NaHS group. Compared with control group, the content of H₂S in plasma was increased by 26% on day 16. Compared with CS+LPS group, the content of H₂S in plasma of CS+LPS+PPG group was decreased by 22% after 30 days. Compared with control group, the protein expression of CSE increased, and no statistical difference among CS+LPS group, CS+ LPS+ NaHS group and CS+LPS+PPG group was observed. Compared with control group, MDA content in the lung tissues was increased by 24% in CS+LPS group, the activity of SOD was increased by 47% and the activity of CAT was increased by 52%. Compared with CS+LPS group, the MDA content in CS+LPS+NaHS group was decreased by 21%, and no statistical difference in the activity of SOD and CAT was observed. The activity of SOD decreased by 33% after given PPG.CONCLUSION: H₂S plays a role as anti-oxidant in the rats with COPD. The CSE/ H₂S pathway may be involved in the development of COPD.     

小果型西瓜新品种苏蜜8号的选育

苏蜜8号是以SW032为母本,以SW058-1为父本配制而成的小果型西瓜一代杂种。早熟,果实发育期约30d(天)。植株生长势中等,耐低温弱光,易坐果。果实高圆形,单果质量1.8~2.3kg。果皮底色浅绿,覆深绿色窄条带,果皮厚0.4~0.5cm。果肉黄色,中心可溶性固形物含量11.0%~12.2%,质地酥嫩,汁液多,纤维少,风味佳。每667m2产量2000~2500kg,适宜长江中下游地区春季大棚栽培。     

喷灌条件下作物光合作用及生长的研究进展

就国内外有关喷灌条件下作物光合作用及生长的研究成果进行了分析和评述,讨论了喷灌条件下作物的生态、生理因子之间的相互关系以及对农田小气候变化的适应机制,指出了该领域研究中存在的问题和今后应加强的研究方向。     

秸秆覆盖量和覆盖时间对党参生长及光合特性的影响

试验以露地不覆盖(CK1)和地膜露头覆盖(CK2)为双对照,设置了移栽覆盖(A1)和出苗覆盖(A2)2个覆盖时间,4500 kg·hm-2(B1)、7500 kg·hm-2(B2)、10500 kg·hm-2(B3)、13500 kg·hm-2(B4)4个覆盖量,采用二因素随机区组设计,研究了秸秆覆盖量和覆盖时间对党参...     

智能化楼宇安防自动监控报警系统的研究

通过介绍智能化楼宇的类型和特点,阐述了智能化楼宇安防自动监控报警系统的组成结构,分析了智能化楼宇安防自动监控报警系统的工作原理及设计原则。通过实习案例论述了应用智能化楼宇安防自动监控报警系统后提高安防效率的重要性。     

欧李离体叶片再生体系的建立

【目的】以欧李试管苗叶片为外植体进行不定芽再生研究,以期为核果类果树的品种改良、基因遗传转化和功能验证等生物技术育种奠定一定的基础,【方法】使用不同的基本培养基和激素组合促进其不定芽再生并建立再生体系。【结果】结果表明,欧李幼芽增殖对基本培养基类型较敏感,改良MS培养基优于MS、F14和WPM。在MS(改良)+NAA 0.1 mg.L-1+BA 0.2 mg.L-1培养基上,增殖系数为5.6,增殖效果较好。离体叶片不定芽再生的最适植物生长调节剂组合为MS(改良)+TDZ 4.0 mg.L-1+IBA 0.2 mg.L-1,再生率达到63.1%,平均每外植体再生芽数为4.9。暗培养10 d可以提高不定芽再生率。再生不定芽1/2 MS(改良)+IBA3.0 mg.L-1培养基上生根率最高,生根率达93.3%。【结论】在改良MS培养基上TDZ与IBA组合诱导不定芽再生的效果最好。     

气象、农业干旱指标综述

介绍了以降水量统计特征作为指标和以降水量、气温统计特征作为指标的气象干旱指标,以土壤含水量、作物旱情、作物需水量、供需水比例、作物水分综合统计特征为指标的农业干旱指标.列出气象、农业干旱指标计算公式,介绍计算方法和干旱指标的详细等级标准,并对各指标计算所需统计资料观测、收集的难易程度,各指标的优缺点、适用性及其适用区域范围评述,为干旱的监测、评估、预警和研究提供依据.     

河北省棉花气候适宜度及其时空变化趋势分析

棉花为河北省重要的经济作物,与其它作物相比对气候比较敏感.选取河北省25个棉花县,对棉花各个发育期气候适宜度及其时空分布进行分析计算.结果表明:棉花从播种到吐絮降水适宜度比温度、日照适宜度明显偏小,尤以播种期和吐絮期最小,且变异系数最大.日照适宜度从播种到吐絮变化不大,较温度和降水适宜度高.多年适宜度的变化趋势为:温度适宜度缓慢上升,降水适宜度缓慢下降,日照适宜度在明显下降,气候综合适宜度同降水一致,在缓慢下降.降水适宜度高值区分布在北部棉区,低值区分布在黑龙港流域的部分地区;温度适宜度低值区在唐山大部,高值区在黑龙港流域;日照适宜度低值区在西部太行山丘陵棉区,高值区在东部棉区.     

籼型杂交水稻恢复系的选育研究进展

回顾了中国籼型杂交水稻恢复系的选育成果，综述了恢复系在品质、产量和抗性等方面的选育研究进展，指出扩大遗传差异与塑造良好株叶形态相结合是今后恢复系选育的目标，分子育种技术在恢复系选育中将起重要作用。     

浙江群众传统灯光围网渔业利用资源状况分析

根据浙江省海洋水产研究所1971~2006年的监测资料及2001~2006年的社会调查资料,对浙江省群众传统灯光围网渔业及其利用资源状况进行了分析。结果表明:由Schaefer和Fox模型估算,现作业渔场的平均最大持续产量为6.52×104t,相应的捕捞努力量为2.62×104组×天;在其发展过程中发生了两次过度捕捞,分别在1986~1988年与2003~2005年。2001~2006年间,2004年的经济效益最为显著,船均利润达23.35万元,而最差的2006年只及它的11.61%。建议今后将船组数量控制在2003年的水平,即300组左右,并将每年的6~7月设为休渔期,鲐鱼的最小可捕体重标准定为75 g。