Mechanism of RhoA on vascular reactivity following hemorrhagic shock in rats

AIM: To observe the mechanisms of RhoA on vascular reactivity following hemorrhagic shock (HS) in rats. METHODS: The superior mesenteric artery (SMA) in rats subjected to hemorrhagic shock was adopted to assay the vascular reactivity via observing the contraction initiated by norepinephrine (NE) with isolated organ perfusion system. Meanwhile, the effects of Rho kinase, myosin light chain phosphatase (MLCP), myosin light chain kinase (MLCK) on RhoA regulating vascular reactivity were observed. The effects of RhoA agonist U-46619 and inhibitor C3 enzyme on the activities of Rho kianse, MLCP, MLCK and phosphorylation of MLC20 in the vascular smooth muscle cells (VSMC) with hypoxia were also measured. RESULTS: As compared to control group, the cumulative dose-response curves of SMA to NE at 2 h after shock shifted to the right, the maximal contractions (Emax) of NE was significantly decreased. RhoA agonist U-46619 increased the vascular reactivity in the late period of shock. C3 enzyme abolished U-46619 induced the increase in the contractile response of SMA to NE. Rho kinase inhibitor Y-27632 decreased U-46619-induced the increase in the vascular reactivity, MLCP inhibitor calyculin further promoted the increase in the vascular reactivity. However, MLCK inhibitor had no effect on the U-46619-induced change of vascular reactivity. After hypoxia, the activities of Rho kinase and MLCK, and the level of MLC20 phosphorylation were decreased, MLCP activity was increased. RhoA agonist U-46619 increased the activity of Rho kinase and phosphorylation of MLC20, decreased the activity of MLCP, but had no effects on MLCK activity. CONCLUSION: RhoA plays an important role in the regulation of vascular reactivity following shock. The mechanism is closely related to regulating the activities of Rho kinase and MLCP, and increasing the phosphorylation of MLC20 in VSMC.     

控释肥残膜对土壤性质和作物生长的影响

通过室内残膜强化试验，研究了控释肥残膜对土壤性质及作物生长（以花生为例）的影响。结果表明，土壤中残膜含量在0.008范围内，控释肥残膜可明显降低土壤的透水性，提高土壤的田间持水量和保水性，随膜含量的增加，当达到0.010时，土壤的田间持水量却明显降低，透水明显加快，土壤保水性也随之降低。另外随土壤中膜含量的增加，土壤容重降低，土壤比重变化不大，土壤孔隙度明显增加。强化试验结果进一步表明，在一定的土壤残膜含量范围内，随膜含量的增加，花生生长也呈增加趋势，其中最适宜花生生长的土壤膜含量范围为0.00426－0.00574。控释肥残膜在一定范围内对土壤和作物是有益的。     

利用SDS-PAGE鉴定转基因小麦HMW-GS的表达类型和后代遗传

采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)在蛋白质水平上对T2～T5代小麦转基因株系高分子量谷蛋白亚基(HMW—GS)的遗传表达及其分离进行了鉴定和分析。结果发现，外源1Dx5和1Dy10基因在T2代部分株系中表达；在有的株系中出现了原亚基(8亚基)的缺失或沉默和新亚基(暂定8^*)的产生，因而检测出2 5 10 12，2 5 7 8^* 10 12，5 7 10，2 7 10 12的多种类型。对2 5 10 12型株系进行了多代跟踪分析，发现其在T2～T5代陆续发生分离，存在于不同单株、不同单穗的子粒和同一单粒后代之间。经筛选，获得了天然不存在的亚基类型为2 5 10 12，2 10 12，2 5 12等稳定表达的种质创新株系。     

Identification of resistance gene analogue markers closely linked to wheat powdery mildew resistance gene Pm31

Specific oligonucleotide primers, designed for the sequences of known plant disease resistance genes, were used to amplify resistance gene analogues (RGAs) from wheat genomic DNA. This method was applied in a bulked segregant analysis to screen for the RGA markers linked to the powdery mildew resistance gene Pm31, introgressed into common wheat from wild emmer. Two RGA markers (RGA200 and RGA390) were found to be closely linked to Pm31 and completely co‐segregating with the marker allele of Xpsp3029 linked to Pm31, with a genetic distance of 0.6 cM. These two RGA markers were then integrated into the formerly established microsatellite map of Pm31 region. The result showed the effectiveness of the RGA approach for developing molecular markers linked to disease resistance genes and demonstrated the efficiency of denaturing polyacrylamide‐gel electrophoresis for detecting polymerase chain reaction polymorphism.     

现有生物质能转换利用技术综合评价

本文介绍了生物质能转换利用的方法,对现有生物质能转换技术进行了比较,运用层次分析法从技术先进性、经济可行性和环境安全性三个方面对现有生物质能转换利用技术进行了综合评价,提出了生物质能转换利用技术的优先发展顺序和发展方向,为我国生物质能转换利用技术的发展和应用提供参考依据.     

用激光剥蚀电感耦合等离子体质谱研究小麦籽粒元素的共分布

增加粮食可食用部分微量营养元素的浓度, 需要更好地了解其在植株, 特别是籽粒内的运输和分布规律。激光剥蚀电感耦合等离子体质谱(laser ablation inductively coupled plasma mass spectrometry, LA-ICP-MS)是一种测定植物组织中元素空间分布的新技术。采用该技术对成熟小麦籽粒中锰(Mn)、铜(Cu)、锌(Zn)和磷(P)的空间分布及其关联程度定量研究。结果表明, 所测元素在籽粒不同部位的浓度分布差异很大。Cu、Zn和P浓度均以糊粉层最高, 胚乳最低, 胚居中, 浓度最大差异分别达15、42和33倍; Mn浓度则以胚最高, 胚乳最低, 糊粉层居中, 浓度最大差异达9倍。籽粒同一部位不同位置的元素浓度亦不相同, 外周胚乳(靠近糊粉层)的元素浓度大于内侧胚乳部位对应元素的浓度, 胚最外侧盾片部位的元素浓度大于胚中间位置的元素浓度, 且各元素趋势一致。比较分析发现, 麦粒不同部位元素的浓度变化存在明显的同步性, 籽粒中P浓度高的部位金属元素(Mn、Cu和Zn)浓度也高。这说明不同元素在向籽粒不同部位运输和积累过程中可能存在密切关联性。     

单生育期水分胁迫对温室葡萄根际土壤酶活性及微生物群落的影响

为明确单生育期水分胁迫对土壤酶活性和微生物群落结构的影响,选取5a生'红地球'鲜食葡萄为试验材料,采用单因素完全随机试验设计,以全生育期充分供水为对照(CK),依次在葡萄5个生育期分别设置轻度(M)和重度(S)两个水分胁迫水平进行大田试验,利用Illumina高通量测序技术分析土壤微生物的群落构成和多样性.结果表明:葡...     

不同灌水处理对小桐子生长及耐盐性的影响

为研究不同灌水处理对小桐子生长及耐盐性的影响,取1 a生小桐子幼苗为试验材料,设置19.80,19.80和39.60(交替灌溉),39.60 mm 3个水平的灌水处理;3个水平的施盐处理:每50 g风干土中NaCl分析纯为0,0.15,0.30 g,并监测小桐子的形态,根区Na⁺环境以及植物体Na⁺累积的情况.结果表明:小桐子有一定的耐盐性,受到每50 g风干土中含有0.30 g NaCl分析纯盐胁迫时,交替灌溉处理更有利于小桐子的生长;随着盐浓度的增大,小桐子根区土壤电导率增大的幅度逐渐减小,同时,盐浓度的增大使土层中Na⁺含量增大的幅度也逐渐减小,小桐子干样中各器官Na⁺含量从大到小依次为叶片、根部、茎;19.80 和39.60 mm交替的灌溉方式比39.60 mm灌溉方式节水25%,但小桐子的生长并未受到影响,且各器官钠离子含量差异不具有统计学意义.本试验条件下,受到不同程度盐胁迫的小桐子,19.80和39.60 mm交替的灌溉方式更有利于小桐子生长,提高其耐盐性.     

基于JAVA的WebGIS森林资源信息管理方法探讨

比较WebGIS 5种实现方式的优劣,并给出基于Java技术的WebGIS框架,讨论了WebGIS在森林资源管理中的应用。     

我国部分省(区)猪圆环病毒3型的分子流行病学研究

2016年美国报道了一种可引起猪皮炎肾病综合征、母猪繁殖障碍、呼吸道及高热等多系统炎症的新型病毒猪圆环病毒3型(Porcine circovirus type 3,PCV-3)。为了解我国PCV-3的感染情况,针对PCV-3保守区设计一对特异性引物,对10个省106份样品进行PCR检测并测序分析。结果表明,10个省(区)中6个省(区)为阳性,地区阳性率60%;66个猪场中感染PCV-3的猪场为12个,猪场阳性率为18.2%;106个样品中检测PCV-3阳性的样品为19例,样品阳性率为18.0%。研究结果为更多了解PCV-3在我国感染情况提供了数据基础。