代谢酶抑制剂PX对除草剂防除稗草的增效作用

为解决除草剂防除困难的问题,采用室内盆栽试验测定了PX、PM和Mn²⁺三种代谢酶抑制剂对精喹禾灵、莠去津、烟嘧磺隆、硝磺草酮和烯禾啶防除稗草Echinochloa crus-galli的增效作用,测定不同用量代谢酶抑制剂PX对这5种除草剂的增效作用及其与莠去津混用后稗草体内谷胱甘肽转移酶（glutathione S-transferase,GST）和细胞色素 P450（cytochrome P450,CYP450）活性的变化。结果显示,在3种代谢酶抑制剂中,代谢酶抑制剂PX对精喹禾灵、莠去津、烟嘧磺隆、硝磺草酮和烯禾啶5种除草剂的增效作用最显著。不同用量代谢酶抑制剂PX对不同除草剂的增效作用不同,其中75 g (a.i.)/hm²代谢酶抑制剂PX对莠去津的增效最佳,株防除效果和鲜重防除效果分别增加了35.33个百分点和37.33个百分点。莠去津与代谢酶抑制剂PX混用后,稗草体内GST和CYP450活性均较对照显著降低。表明除草剂与代谢酶抑制剂PX混用能增加除草剂对稗草的防除效果,可以达到减施除草剂的目的。     

Exploring the effectiveness of in ovo feeding of vitamin C based on the embryonic vitamin C synthesis and absorption in broiler chickensOA

Background: Many researches about in ovo feeding(IOF) of vitamin C(VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.Results: Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase(GLO) expression was lower during pre-hatch development than that during p...     

Identification,Characteristics and Function of Phosphoglucomutase (PGM) in the Agar Biosynthesis and Carbon Flux in the Agarophyte Gracilariopsis lemaneiformis (Rhodophyta)

Agar is widely applied across the food, pharmaceutical and biotechnology industries, owing to its various bioactive functions. To better understand the agar biosynthesis in commercial seaweed Gracilariopsis lemaneiformis, the activities of four enzymes participating in the agar biosynthesis were detected, and phosphoglucomutase (PGM) was confirmed as highly correlated with agar accumulation. Three genes of PGM (GlPGM1, GlPGM2 and GlPGM3) were identified from the G. lemaneiformis genome. The subcellular localization analysis validated that GlPGM1 was located in the chloroplast and GlPGM3 was not significantly distributed in the organelles. Both the GlPGM1 and GlPGM3 protein levels showed a remarkable consistency with the agar variations, and GlPGM3 may participate in the carbon flux between (iso)floridoside, floridean starch and agar synthesis. After treatment with the PGM inhibitor, the agar and floridean starch contents and the activities of floridean starch synthase were significantly decreased; products identified in the Calvin cycle, the pentose phosphate pathway, the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cycle were depressed; however, lipids, phenolic acids and the intermediate metabolites, fructose-1,6-phosphate were upregulated. These findings reveal the essential role of PGM in regulating the carbon flux between agar and other carbohydrates in G. lemaneiformis, providing a guide for the artificial regulation of agar accumulation.     

通过考核认证提高警犬实战技能

2006年,辽宁省公安厅作为片区警犬资格认证的试点单位,分别于5月17日至6月12日、11月8日至9日,两次集中组织进行了警犬资格认证考核。全省公安机关共有133头警犬参加考核,占全省163     

高淀粉酿造高粱新品种晋粱 112 号的选育

晋粱 112 号是山西农业大学高粱研究所以河北承德市农林科学院（原河北承德地区农科所）引进的不育系承 16A为母本,以通过组培育种技术选育的优良恢复系 R111 为父本,在山西农业大学高粱研究所东白试验基地选育而成,2023 年通过农业农村部非主要农作物品种登记,登记编号为 GPD 高粱（2023）140102。2021-2022 年晋粱 112 号 2 年适应性试验平均产量达 9583.6kg/hm2,比对照晋杂 34 号平均增产 11.7% ;生育期 128.8d,幼苗绿色,芽鞘绿色,株高 169.7cm,穗长 30.9cm,穗纺锤形,中紧穗型,红壳褐粒,穗粒重 85.5g,千粒重 27.8g。该品种具有淀粉含量高、抗旱、抗丝黑穗病、丰产性好等优点,适宜在无霜期 130d 以上中晚熟地区（山西太原、晋中、长治,山东及新疆昌吉等）种植。     

用PCR检测副溶血性弧菌耐热溶血素基因

根据副溶血性弧菌的耐热溶血素基因（ｔｄｈ）设计了２对引物，经聚合酶链式反应（ＰＣＲ）检测，所有１８株神奈川试验阳性副溶血性弧菌均呈阳性反应，而６株神奈川试验阴性副溶血性弧菌呈阴性反应，其他９种（３４株）弧菌和８株其他革兰氏阴性菌也呈阴性反应。检测敏感性可达２ＣＦＵ的副溶血性弧菌。     

当前我国草原工作面临的形势与任务

草原是我国陆地最大的绿色植被系统,加强草原保护建设意义重大。最近,国家对新时期牧区工作做出重大部署,草原工作迎来了新的契机。本文对草原保护建设利用的现状进行了概括和总结,结合2011年出台的草原相关新政策,提出了今后草原保护建设的工作措施。     

关爱动物健康,关注食品安全

茶,久饮,可以益思、可以养性;茶馆,常往,可品茶论道,道尽天下时事……市场就如此茶,需时刻关注、不断评析,久之,便可把脉市场风云,运筹帷幄。     

云南某农村猪戊型肝炎病毒分子流行病学调查

目的了解昆明市某猪场戊型肝炎病毒感染情况,进一步探究病毒基因型及分子进化情况,为戊型肝炎防控提供参考。方法从该猪场随机采集39份新鲜粪便,用逆转录巢式聚合酶链反应对HEV ORF2部分基因片段扩增、克隆及测序;应用生物信息学软件进行同源性分析并构建系统进化树。结果PCR检测结果发现：该猪场核酸阳性率达35.9% (14/39)。同源性分析结果表明：14株流行毒株核苷酸序列与GenBank中HEV I~IV 型参考株的同源性分别为77.4%~83.9%、81.0%~83.2%、78.8%~86.9%和90.5%~98.5%,全部流行株均属基因IV型。进化分析结果表明：YN-KM-9与该猪场其他流行毒株同源性较远,应属不同基因亚型。结论该猪群HEV IV感染率较高,对公共卫生安全构成潜在威胁。     

不同种源刨花润楠苗期生长及叶片性状变异的研究

以刨花润楠主要分布区的14个种源为研究对象,在广州增城开展种源试验,通过对14个种源的刨花润楠苗高、地径、叶面积、叶周长等性状进行观测,分析不同种源苗期性状的遗传变异规律。结果表明：3个生长性状种源间和种源内变异系数相当,其种源间和种源内生长性状变异均较丰富;8个叶片性状种源间的变异系数为17.52%～44.71%,不同种源间叶片性状亦变异明显;生长性状的种源遗传力均大于0.750,说明在一定程度上刨花润楠的苗高、地径等苗期生长性状受自身遗传控制,且不同种源间变异明显,种源选择潜力较大。刨花润楠苗期生长性状的地理及气候变异趋势不明显,表现出随机现象;苗木生长性状与叶片大小呈负相关,即叶片越大、叶柄越长,苗木生长有变慢的趋势;对种源苗期性状进行聚类,可将刨花润楠14个种源划分为5个类群;刨花润楠苗期生长及叶片性状有一定的地理趋势,但也有一定的随机性。以地径为标准对刨花润楠优良种源进行筛选,初步选出永春、兴安、昭平3个种源作为适宜广东地区造林的优势种源。