关中平原小麦产量对气候变化区域响应的评价模型研究

根据关中地区宝鸡、西安、渭南与咸阳 4地 (市 )的 1 949～ 1 999年的逐年小麦单产记录序列以及 4地 (市 )的气象观测站点自建站以来至 2 0 0 0年近 5 0年的气象记录序列 ,对关中地区小麦产量与年均温、年降水作相关分析 ;探讨了关中地区小麦单产对气候变化区域响应的评价模型。结果发现 :关中平原气候具有暖干化趋势 ;随着气温变暖 ,小麦产量增加幅度减小 ;小麦产量对降水波动的响应比对气温波动的响应显著。     

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.     

陕甘宁老区脆弱生态环境定量评价——以榆林、延安两市为例

位于黄土高原中部的陕甘宁老区生态环境极为脆弱 ,近年来由于气候、人类开发资源等自然和人为原因 ,使生态环境的脆弱程度升高。选定年降水量、年均温、蒸发量等 8个指标 ,定量评价各县 1 970 - 2 0 0 0年的脆弱度状况 ,结果表明榆林、延安两市生态环境整体脆弱 ,脆弱度存在空间差异但差异不明显 ,时间段上的波动幅度不大。陕甘宁老区脆弱生态环境具有不稳定性 ,对外界干扰较敏感。     

双孢蘑菇性亲和性相关分子标记的初步筛选

以传统的形态，生理生化分析和最新的DCS－PDMA性亲和性测定方法为基础， 结合群体分离分析和RAPD技术来源于同一双孢蘑菇异核体菌株的12个不育同核原生质体个体进行分析，筛选与性亲和性相关的分子标记。研究结果表明，供试的12个不育同核原生质体个体被分成两大类性亲和性类型，其中一类（A^ ）包括不育同核原生质体个体B、C、D、E、F、G、H、I、J、L，另一类（A^-）则仅仅包括不育同核原生质体个体K和M，同时筛选到一个与性亲和性相关的分子标记OPA16 1500。从而为间地利用双孢蘑菇本身特有的交配型作标记来指导杂交育种工作和进一步将性亲和性基因定位分离克隆奠定了坚实的基础。     

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C₁, group treated by ginsenosides C₂. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C₁ and C₂ were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C₁ and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.     

Effects of riboflavin and ascorbic acid on apoptosis and proliferative inhibition of mouse thymocytes induced by deoxynivalenol in vivo

AIM: To explore the effects of riboflavin and ascorbic acid on the apoptosis induced by deoxynivalenol(DON) in mouse thymocytes. METHODS: The effects of riboflavin and ascorbic acid on the apoptosis and proliferation inhibition of thymocytes induced by DON in KM mice were studied with animal experiment, DNA agarose gel electrophoresis and flow cytometric DNA content analysis. RESULTS: Apoptosis rate of thymocytes in DON (4 mg/kg) treated group was13.73%±15.3% The percentages of apoptosis in riboflavin (1.25 mg/kg-10mg/kg) and ascorbic acid (25 mg/kg-100mg/kg) pretreated thymocytes groups were significantly lower than that in DON group (P <0.05). The result of DNA agarose gel electrophoresis showed that the characteristic ladder pattern of apoptosis was found in DON-treated thymocytes, but not in control and riboflavin pretreatment and ascorbic acid pretreatment groups The significant differences in proliferation index were not found among DON-treated thymocytes and riboflavin and ascorbic acid-pretreated thymocytes CONCLUSION: Pretreatment with riboflavin and ascorbic acid inhibit apoptosis of mouse thymocytes induced by DON in certain extent and have no effect on proliferation inhibition by DON.     

蛹虫草人工优质高产栽培技术研究

本试验通过对四个人工分离蛹虫草品种的菌丝体生长情况、子实体产量进行对比分析，结果显示：沈阳人工分离种（S）为优质品种，武汉（W）、河南（H）、北京（B）种为淘汰种。以大米加4个蚕蛹为基质的培养基子实体生物学效率最高；液体菌种生长周期短，生物学效率较高。     

典型光活化毒素α-三噻吩对棉铃虫和亚洲玉米螟Na~+-K~+-ATPase的抑制作用

以棉铃虫 H elicoverpa armigera和亚洲玉米螟 Ostrinia furnacalis为试虫 ,建立 Na+- K+-ATPase最佳反应系统 ,研究典型光活化毒素 α-三噻吩 (简称 α- T)对 Na+- K+- ATPase的影响。棉铃虫 Na+- K+- ATPase活力最佳测试条件为酶源蛋白浓度 6μg/ m L ,反应温度 35～ 4 0℃ ,反应时间6min;亚洲玉米螟则为酶源蛋白浓度 8μg/ m L,反应温度 35℃ ,反应时间 6min。近紫外光照 (30 0～4 0 0 nm )对棉铃虫和亚洲玉米螟离体 Na+- K+- ATPase活力基本没有影响 ,但对活体活力有很强的抑制作用。光照和无光照条件下 ,α- T对两种昆虫离体和活体 Na+- K+- ATPase活力均有不同程度的抑制作用。光照组 α- T对亚洲玉米螟 Na+- K+- ATPase的抑制率高于无光照组 ,且处理浓度或剂量越高 ,其抑制率越大 ;对棉铃虫 Na+- K+- ATPase抑制作用不显著     

达县分葱田甜菜夜蛾发生规律初步研究

采用系统调查方法研究了达县地区分葱田甜菜夜蛾的生活史、发生与环境的关系。甜菜夜蛾在达县分葱田1a发生6代,以三至五代为害最重,世代重叠。其数量消长与虫源、夏秋季气候、天敌、栽培管理等因素密切相关。通过调查分析提出本县初始虫源非本地虫源。     

迟菜心新品种特青迟心4号的选育

特青迟心4号为迟熟种，株型矮壮，叶色深绿，薹色青绿，有光泽，菜薹匀条实心，主薹高25．4cm，薹粗2．10cm，单薹重62．58，商品率高，较耐霜霉病，品质脆嫩，风味好，适播期10月—翌牟2月，播种至初收50—55天，可连续采收20-25天，667m^2产量1000—1400kg。