Inductive expression of the NOD1 signalling pathway in chickens infected with Salmonella pullorum

1. The aim of this study was to describe the role of Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) receptor signalling in chicken.

2. Tissue-specific expression analysis of NOD1, receptor-interacting serine-threonine kinase 2 (RIPK2), nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase 11 (MAPK11 or p38) by quantitative real-time PCR (qRT-PCR) revealed their wide distribution in various organs and tissues.

3. Salmonella pullorum infection activated NOD1 receptor signalling in vivo and in vitro, resulting in significant induction of downstream signalling molecules RIPK2, NF-κB/p65, MAPK11/p38 and the effector molecules IL-1b and IL-8.

4. Activation of NOD1 by its agonist bacterial γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) in HD11 cells induced the adapter molecular RIPK2 and activated the NF-κB/p65 and MAPK11/p38 pathways, resulting in an increase in IL-8 but not IL-1β. Additionally, inhibition of NOD1 using NOD1-shRNA resulted in downregulation of RIPK2, MAPK11 and IL-8, while NF-κB/p65 and IL-1β were unaltered.

5. These results highlight the important role of NOD1 receptors in eliciting the innate immune response following pathogenic invasion in chicken.     

紫花苜蓿草对辽宁绒山羊营养价值的研究

为了评价紫花苜蓿干草对不同类别辽宁绒山羊的营养价值,研究不同类别绒山羊对各种营养素消化利用能力,开展了本次试验研究。选择成年母羊、育成母羊、成年种公羊、育成公羊和羔羊各4只,依次设为1、2、3、4、5组,只饲喂苜蓿干草。测定不同类别辽宁绒山羊对苜蓿草各营养物质的消化、代谢和利用能力,评价苜蓿草对辽宁绒山羊的营养价值。结果表明,成年母羊对苜蓿草的纤维类和脂肪营养的消化率均高于育成公母羊,而育成公母羊对苜蓿草能量的表观代谢率和利用率均高于成年公母羊。羔羊对苜蓿草能量利用率均低于成年公母羊和育成公、母羊,并与育成公羊差异极显著（P〈0．01）;对苜蓿草钙、磷、纤维类营养、粗蛋白和粗脂肪的表观消化率也低于其他类绒山羊。     

河南警犬参与侦破命案4例

2007年1月26日,河南省郸城县东风乡发生一起入室抢劫杀人案,受害人侯某被人杀死在自家床上,家中被抢走现金9700元。接指令后,淮阳县公安局带犬民警徐奎携警犬“追命”赶赴现场。在带犬民警的指挥引导下,     

大豆市场中长期走势向好

CBOT大豆价格自10月初开始上涨以来。涨幅已达到130美分／蒲式耳．本轮上涨行情跟大豆当前利空的供需基本面并不相符。行情的上涨最初是由小麦带动，随后则是由玉米带动，由于玉米长期前景看好，且美国玉米和大豆又处在同一生长带，玉米价格的上涨将促使农民多种玉米少种大豆．这样的前景终将导致大豆的供需平衡趋紧。因此，为了保证大豆面积不会大幅下降，大豆价格就只有跟随玉米上涨．这就造成了大豆出现本轮上涨行情。鉴于玉米价格长期看好的前景不会改变．我们认为大豆价格也进入了长期牛市行情之中．但是短期内不排除期价步入回调。下面从几个方面来论证我们的观点。     

一株无血凝性兔出血症病毒的分离鉴定及其衣壳蛋白VP60基因的克隆和序列分析

从临床病料中分离鉴定出1株无血凝性的致病性兔出血症病毒（RHDV），命名为Yaan-1。HA试验测得该毒株原代及经兔体传3代后的肝毒在25℃、37℃和4℃时均无血凝性；但在琼脂扩散试验和对流免疫电泳中均可见分离株与常规RHDV高免血清形成清晰的沉淀线；电镜观察可见30nm左右的病毒粒子。采用RT-PCR方法将Yaan-1株的衣壳蛋白VP60基因进行了克隆测序，序列分析表明VP60基因序列全长1740bp，编码579个氨基酸，测序结果提交GenBank（注册号为DQ069280），并与世界各国的常规RHDV分离株进行比较，结果表明核苷酸同源性为90．0％～98．0％，氨基酸同源性为94．1％～99．0％，氨基酸变异多发生在衣壳蛋白C、E区；同时对Yaan-1株的分子量、等电点、疏水性、跨膜区等分子结构特征进行了分析。本研究首次报道了无血凝性RHDV中国分离株，丰富了地方毒株资源，为明确我国地方株的分子流行病学，以及对VP60蛋白分子特征研究提供了新材料。     

浅谈鸡球虫病的免疫

鸡球虫病是一种危害性较大的肠道寄生虫病,多发于3个月龄以内的仔鸡.尤以15～45日龄的雏鸡最易感染,近年来,球虫疫苗应用,使工厂生产的养鸡场在鸡球虫病控制方面取得显著成绩.笔者就鸡球虫病免疫在生产实践中的应用,作肤浅论述.     

无锡市猪肉批发市场猪肉的检验和卫生处理

做好市场猪肉的检验工作至关重要,它关系到广大人民群众的身心健康.本人结合市场的实际情况,从这几年的工作实践中,归纳出以下几条关于本市场猪肉的检验要点,供同行参考.     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.