简述湿地公园总体布局及其保护对策

从3个方面分别介绍了吉林扶余大金碑湿地公园的概况、总体规划布局及保护利用对策。     

面向猕猴桃产业链的联盟链隐私交易方案

针对猕猴桃产业联盟链交易中金额与身份的双重隐私保护问题，提出一种基于⁺HomElG零知识证明(⁺HomElG zero knowledge proof,⁺HomElG-ZKProof)和SM2的猕猴桃产业联盟链隐私交易方案。首先，转账方利用⁺HomElG加密交易金额后将其发送给接收方，接收方基于SM2签名确认交易后发送给转账方；其次，转账方基于⁺HomElG-ZKProof对交易金额相关密文生成零知识证明证据，基于SM2可链接环签名对交易金额相关密文和交易双方身份分别生成环签名，与接收方SM2签名一起经系统层Raft共识打包上链；然后，由监管节点验证SM2签名、两次环签名及链接性后确认交易双方身份，用户节点间在应用层使用PBFT共识验证交易金额相关密文、环签名及交易金额相关零知识证明证据后确认交易的有效性；最后，由监管节点将有效交易区块编号经系统层Raft共识上链并更新账户余额。分析表明，该文方案具有抗篡改攻击、抗公钥替换攻击、抗假冒攻击以及匿名性，安全性较高；测试结果表明，该...     

Evolution Analysis About Soybean MIR166 Family

MicroRNA(miRNA) is a class of important regulating non-coding small molecular RNA. The gma-MIR166 gene family consists of 21 members and their expression patterns diversify widely. It is important to analyze the evolution of gma-MIR166 gene family in order to understand the evolutionary mechanisms of miRNAs in soybean. In this study, we implemented soybean wide genome block analysis, the molecular phylogeny of gma-MIR166 and block analysis of gma-MIR166 family. The results showed that both chromosome big segmental duplications and tandem duplications were main reasons contributed to the expanding of gmaMIR166 gene family. These findings suggested that gma-MIR166 gene family might originate from one or two ancient miRNA genes. The results of research provided a support for evolutionary study of miRNAs in soybean and related species in Fabaceae.     

Effects of phytosterol ester on aortic aging and expression of related genes in rats

AIM: To explore the protective effect of phytosterol ester (PSE) on aortic aging in rats. METHODS: The female SD rats (12 months old, n=42) were randomly divided into control group, model group and PSE group. During the experiment, the rats in control group, model group and PSE group were treated with basic feed, high-fat diet (HFD) and HFD with 2% PSE (W/W) for 6 months, respectively. The morphological changes of the aorta were observed by HE staining and Masson staining, and the absolute area of smooth muscle cells and collagen fiber in the vascular wall were measured by image analysis. The levels of advanced glycosylation end products (AGEs), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) in the plasma were detected. The expression of silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor γ (PPARγ) at mRNA and protein levels in the vascular tissue was determined by real time PCR and Western blot, respectively. RESULTS: PSE significantly lowered plasma TC and LDL-C, and increased plasma HDL-C level (P<0.05), but had no effect on plasma TG level. PSE significantly attenuated the thickening of intima and media of aging aortic, and decreased the migration of vascular smooth muscle cells (VSMC) and the amount of VSMC and collagen fiber in the aorta (P<0.05). PSE significantly reduced the contents of AGEs and MDA (P<0.05), but had no effect on the activity of SOD and CAT in the plasma. PSE also down-regulated the expression of PPARγ and up-regulated the expression of SIRT1 (P<0.05). CONCLUSION: PSE is able to attenuate the senescence process in the aorta by reducing the production of reactive oxygen species in plasma, and activating SIRT1, or inhibiting the expression of PPARγ in vascular tissues.     

油用亚麻可溶性糖、脂肪含量与硬脂酰-酰基载体蛋白脱氢酶基因表达相关性分析

为研究油用亚麻叶片、果球4个发育阶段硬脂酰-酰基载体蛋白脱氢酶基因(sad)表达水平与可溶性糖含量、脂肪含量之间的关系,以3个已知亚麻酸含量相对不同的油用亚麻品种为试验对象,应用q RTPCR技术对不同发育时期组织中sad基因的相对表达量与可溶性糖含量以及脂肪含量之间的关系进行分析。结果表明,在油用亚麻叶片和果球的不同发育阶段sad基因的表达模式符合正态分布,在品种和时期之间表达量差异明显。可溶性糖的含量在果球和叶片中差异明显,相关性分析结果显示,在sad基因表达量增加时,可溶性糖含量相对减少;脂肪含量与sad基因的表达量呈极显著正相关。油用亚麻中sad基因表达直接影响油用亚麻可溶性糖和脂肪含量,进而影响亚麻油的品质。     

陆地棉ZF-HD蛋白的全基因组分析

ZF-HD(Zinc finger-homeodomain)蛋白属于同源异型盒蛋白家族,在动植物发育过程中起重要的作用。利用生物信息学的手段,研究陆地棉标准系TM-1基因组中ZF-HD蛋白的数目、亚细胞定位、染色体分布、基序、进化关系和组织表达情况。TM-1中共有35个Gh ZHD蛋白成员,且大部分成员定位到细胞核内;分布在20条染色体和2条Scaffold上,且具有11对直系同源基因;进化树分析表明,TM-1基因组中的ZF-HD蛋白大致分为6个小组,每个小组成员间具有类似的基序类型和排列顺序;大部分Gh ZHD基因在胚珠和纤维组织中表达,还有部分基因在根、茎、花、蕾和分生区中表达,在叶和愈伤组织中表达的基因最少。     

低温胁迫下草坪草抗寒性的研究进展

通过介绍低温对草坪草的影响和作用机理,系统地综述了在低温胁迫下草坪草的光合特性、水分生理代谢、细胞质膜相对透性、保护酶系变化、碳水化合物贮藏、可溶性蛋白质含量、以及脂肪酸的变化在低温胁迫方面的研究进展,提出了今后进行草坪草抗寒性研究的趋势和建议。     

关于生物类课程实施创新教育的几点思考

对如何在生物教学中培养学生的创新能力进行了综合的论述,并就教学活动中利于培养学生创新意识和创新能力的4个环节进行了逐一说明,指出了创新教育的必要性,以期为生物类课程教育提供借鉴和参考。     

酒石酸泰万菌素对特定畜禽疫病防治功效研究进展

酒石酸泰万菌素(简称泰万菌素)系大环内酯类动物专用抗生素。本文综述了泰万菌素防治敏感细菌感染、支原体病、猪痢疾以及猪繁殖与呼吸综合征等畜禽疫病的功效研究成果,证实泰万菌素对于防治上述畜禽疫病功效显著。近年来研究发现,泰万菌素活性不同于其他类抗生素,可作为抗病毒药物使用,特别适用于对猪繁殖与呼吸综合征的治疗。泰万菌素可抑制病毒复制,缩短病毒血症周期,降低死亡率,减轻临床症状;同时还可通过降低母猪流产率,提高产健康仔猪数以及改善母猪、仔猪病毒血症等,达到控制病毒垂直传播的目的。在当前日益关注兽药残留、动物源细菌耐药性的社会背景下,泰万菌素制剂能很好满足畜禽养殖场用药需求。     

复杂疾病的遗传咨询技术

复杂疾病大面积爆发使人们的生活健康面临着重大挑战。在这一环境下,遗传咨询在人们的生活中占据了越来越重要的地位。依据基因与环境互作共同影响个体健康的原理,咨询者在遗传咨询师的指导帮助下合理解决各种遗传学问题可以有效降低复杂疾病的发病率。目前国内的遗传咨询发展尚处于萌芽期,随着遗传咨询在健康领域的地位上升,该技术在未来将会进一步推广应用。