Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2)

Bullous pemphigoid (BP) is an autoimmune subepithelial blistering dermatosis of humans, dogs, cats and pigs. It is characterized by skin-fixed and circulating IgG autoantibodies that target one or both BP antigens. An immunological homologue of BP in humans was diagnosed in two horses with cutaneous and mucosal ulcerations as well as microscopic subepithelial vesiculation. Immunological investigations revealed similar findings for both the horses. Direct immunofluorescence demonstrated the presence of IgG deposited linearly at the dermoepidermal junction in mucosal and skin biopsy specimens. Indirect immunofluorescence testing confirmed the existence of circulating basement membrane-specific IgG autoantibodies. Using intact and salt-split epithelial substrates, serum IgG were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoblotting and ELISA corroborated that the IgG from affected horses, but not those from normal controls, exhibited high immunoreactivity against the NC16A extracellular domain of type XVII collagen (BPAG2, BP180). Equine BP could be proposed, therefore, as another spontaneous model of this most common basement membrane autoimmune dermatosis of humans.     

紫苏-月苋草复合提取物对肉种鸡生产性能的影响

试验选用1日龄AA肉用种鸡17000羽,随机分成对照组(饲喂基础日粮)和试验组(在基础日粮中添加0.25g/kg紫苏-月苋草复合提取物),研究紫苏-月苋草复合提取物对肉种鸡生产性能和繁殖性能的影响。试验结果表明:添加该复合提取物后,试验鸡育成期体重、均匀度显著提高,死淘率降低,产蛋率和平均蛋重有较大的提高,受精率和入孵蛋健雏率分别提高1.04个百分点和5.35个百分点。     

LRH-3、PMSG、HCG对人工授精母蓝狐产仔效果影响的比较(Ⅲ)

通过PMSG +LRH - 3和PMSG +HCG的组合使用 ,结果表明 ,使用PMSG +LRH - 3的试验组母狐的产仔率比对照组提高了 2 0 % ,胎平均产仔数提高了 0 2只。而使用PMSG +HCG组合母狐的产仔率与对照组相同 ,但胎平均产仔数提高了 0 5只。     

Vero E6细胞酵母双杂交cDNA文库的构建及鉴定

To seek out proteins which interact with structural proteins of porcine epidemic diarrhea virus （PEDV） in Vero E6 cells and study the mechanisms of viral infection.We created a Vero E6 cDNA yeast hybrid library. The total RNA of Vero E6 was extracted using Trizol method, and double strands cDNA was synthesized by SMART technique. We created a Vero E6 cDNA library by using homologous recombination in yeast. The results showed that the titer of cDNA library was 9.7×10⁸ CFU with the average of inserted fragments about 1.6 kb, and the recombination rate was 87%. These data indicated that the yeast two-hybrid cDNA library of Vero E6 was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PEDV.     

Inductive expression of the NOD1 signalling pathway in chickens infected with Salmonella pullorum

1. The aim of this study was to describe the role of Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) receptor signalling in chicken.

2. Tissue-specific expression analysis of NOD1, receptor-interacting serine-threonine kinase 2 (RIPK2), nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase 11 (MAPK11 or p38) by quantitative real-time PCR (qRT-PCR) revealed their wide distribution in various organs and tissues.

3. Salmonella pullorum infection activated NOD1 receptor signalling in vivo and in vitro, resulting in significant induction of downstream signalling molecules RIPK2, NF-κB/p65, MAPK11/p38 and the effector molecules IL-1b and IL-8.

4. Activation of NOD1 by its agonist bacterial γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) in HD11 cells induced the adapter molecular RIPK2 and activated the NF-κB/p65 and MAPK11/p38 pathways, resulting in an increase in IL-8 but not IL-1β. Additionally, inhibition of NOD1 using NOD1-shRNA resulted in downregulation of RIPK2, MAPK11 and IL-8, while NF-κB/p65 and IL-1β were unaltered.

5. These results highlight the important role of NOD1 receptors in eliciting the innate immune response following pathogenic invasion in chicken.     

河南警犬参与侦破命案4例

2007年1月26日,河南省郸城县东风乡发生一起入室抢劫杀人案,受害人侯某被人杀死在自家床上,家中被抢走现金9700元。接指令后,淮阳县公安局带犬民警徐奎携警犬“追命”赶赴现场。在带犬民警的指挥引导下,     

大豆市场中长期走势向好

CBOT大豆价格自10月初开始上涨以来。涨幅已达到130美分／蒲式耳．本轮上涨行情跟大豆当前利空的供需基本面并不相符。行情的上涨最初是由小麦带动，随后则是由玉米带动，由于玉米长期前景看好，且美国玉米和大豆又处在同一生长带，玉米价格的上涨将促使农民多种玉米少种大豆．这样的前景终将导致大豆的供需平衡趋紧。因此，为了保证大豆面积不会大幅下降，大豆价格就只有跟随玉米上涨．这就造成了大豆出现本轮上涨行情。鉴于玉米价格长期看好的前景不会改变．我们认为大豆价格也进入了长期牛市行情之中．但是短期内不排除期价步入回调。下面从几个方面来论证我们的观点。     

浅谈鸡球虫病的免疫

鸡球虫病是一种危害性较大的肠道寄生虫病,多发于3个月龄以内的仔鸡.尤以15～45日龄的雏鸡最易感染,近年来,球虫疫苗应用,使工厂生产的养鸡场在鸡球虫病控制方面取得显著成绩.笔者就鸡球虫病免疫在生产实践中的应用,作肤浅论述.     

无锡市猪肉批发市场猪肉的检验和卫生处理

做好市场猪肉的检验工作至关重要,它关系到广大人民群众的身心健康.本人结合市场的实际情况,从这几年的工作实践中,归纳出以下几条关于本市场猪肉的检验要点,供同行参考.     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.