植物表达载体pGMAR-BADH的构建

将甜菜碱醛脱氢酶基因BADH和植物表达载体pGMAR通过内切酶BamHI和KpnI双酶切,T4连接酶进行连接反应。重组质粒在大肠杆菌菌株DH5α内扩增,提取并纯化质粒。经鉴定,基因BADH已被完整、正确的插入到pGMAR载体中,并成功将BADH插入到载体pGMAR的两个MAR序列之间。     

A Furry Synthetic Evaluation Model in Capital Structure Decisions

This paper analyzes the limitation of regarding the weighted average cost of capital as the optimal standard of capital structure in the traditional capital structure decision-making method, and analyzes the view that the capital structure is affected by many factors,such as earning power, long-term stability of operating, the attitudes held by owners and operators, the attitudes held by creditors and financial risk. With the example, the affecting factors of the capital structure is analyzed. The fuzzy synthetic evaluation model are employed to decision-making of the capital structure. This article also shows the key point of its application.     

Existence of an Arbitrage about Portfolio in an Unequilibrium Market

This article has discussed the problem of an arbitrage about an portfolio in an unequilimarket and got a determinable theorem about existence of an arbitrage about an portfolio. According to this theorem,an arbitrage about portfolio is not existing if there exists a respect martignal measure.A structural result about existence of arbitrage about an portfolio in a market is gotten. All results represent the existence of an arbitrage about an portfolio is related closely to the type of market.     

A Caculation Method of Portfolio Investment under the Risk Mininiz

According to the security investment theory of Markowitz,this paper presents aCaculation method of portfolio investment under the risk minimization,and some mathematic expres-sions of the efficient frontier for portfolio investment are conducted.Based on these,the efficientfrontier is proved as a parabola,and a practical caculation process is given.     

高含沙水流水轮机转轮的改型与抗磨蚀研究

进行高含沙水流水轮机转轮的改型研究，关键是要从正确确定转轮设计参数和轴面流道型式入手，根据三维水力设计理论，考虑导叶与转轮匹配关系进行叶型设计，针对不同破坏机理选择强磨蚀区采用金属喷涂或非金属保护等综合措施，使水轮机在高舍沙水流中运行时出力增加、抗磨蚀能力提高。     

我国北方地区节水农业技术水平及评价

在系统分析我国节水农业技术水平现状的基础上 ,对各类节水技术的差距和存在问题进行了探讨 ,提出了各类技术亟待研究解决的若干重点问题。并指出建立以渠道防渗技术、管道输水技术及地面灌水技术为核心 ,符合国情的节水农业技术体系已迫在眉睫。     

河北省主产区苹果园肥料投入现状分析与评价

为探明河北省不同产区苹果园施肥现状及其与产量的关系,采用实地调查的方式,调查了太行山和燕山两大产区苹果园肥料投入种类、投入量及产量,并进行了统计分析。结果表明:河北省主产区79.17%的苹果园施用有机肥,以羊粪、鸡粪和牛粪为主;所有果园均施用化肥,以三元复合肥为主,N、P₂O₅、K₂O投入量分别平均达到了1 106.87、689.88、902.47 kg/hm²,处于严重过量水平。太行山产区有机肥投入量高于燕山产区,施用有机肥的果园比率较燕山产区高48.19%,而燕山产区无机肥投入量高于太行山产区,2个产区间苹果产量无显著差异。进一步分析发现,N、P₂O₅、K₂O投入量分别处于600~900、300~600、300~600kg/hm²区间时苹果产量最高,其中分别只有27.97%、27.97%、27.12%的农户施肥量处于该范围,且不同产区间存在较大差异。综上,河北省苹果园整体养分投入水平较高,变异较大,无机肥种类单一,占比较高;不同产区间养分投入量、投入种类存在较大差异,但苹果产量差异较小。     

浅谈草坪病害及其防治

草坪越来越被广泛地应用于各种绿地之中,草坪的病害也时常发生,作好草坪病害的防治可以大大提高草坪的观赏质量。文章介绍了几种常见的草坪病害发生的情况和防治措施。     

植物造景若干问题的探讨

植物是园林要素的一部分,可以改善生态环境,创造优美的艺术空间,植物造景不是简单的植物堆积和返朴归真,它有其自身的理论,应该遵循一定的原则。     

盐生植物做除虫杀菌剂应用展望

盐生植物做除虫杀菌剂,用于生产绿色食品,解决农药在植物体内的残留问题,使人们重新认识植物源除虫杀菌剂的作用。重点阐述了可用于除虫杀菌的盐生植物的种类和性能,盐生植物除虫杀菌剂的制作及使用方法。面对在农产品贸易中苛刻的农药残留标准,使用植物源农药,减少化学农药的施用量,生产绿色无公害食品,盐生植物具有极大的潜在威力。