柚子花芳香油超临界CO2萃取研究

介绍了新鲜柚子花中芳香性成分超临界CO2萃取分离工艺和分析检测方法,重点探讨了压力、温度、时间对萃取率的影响.应用正交试验优化得出:影响萃取的主次因素依次为为萃取压力、萃取温度、萃取时间;较佳工艺参数为:压力18MPa,温度50℃,时间90 min,流量25 L/min,得到超临界柚子花芳香油的萃取率高达2.7‰.应用气相色谱-质谱联用仪共鉴定出39个组分,占总芳香油的91.281%.通过对柚子花的深度加工研究,为开发高附加值的柚子花香精提供科学依据.     

长汀县退化劣质"老头松"改造效果分析

采用挖穴施肥和条沟草带2种方法,在2个不同的地点对劣质马尾林进行改造试验,从林木年抽梢、材积、覆盖和地表草灌多样性进行分析.结果表明:经过3年改造的马尾松抽梢总长在1.6m以上,比对照0.95m增长0.65m以上,年均多增长0.22m;材积增长12.7m3/hm2,比对照3.4增长9.3m3/hm2,年均增加3.1m3/hm2;覆盖度也增加32%～47%,比对照分别增加19%和32%;同时促进了生物多样性,草灌植被均增加10种以上.     

振动筛式花生收获机的设计与试验(简报)

介绍了4H-800型振动式花生收获机的总体结构、工作原理、技术特点以及关键部件结构与工作参数设计等。该设备主要由挖掘铲、单侧立式切割器、振动筛、驱振组件、行走轮、动力传动装置及机架等组成,与11～13.2 kW拖拉机配套使用,一次完成两行花生收获。试验和示范应用表明,该机具有作业顺畅、运行可靠、清土率高、损失率低等特点,纯生产率达0.1 hm2/h、总损率1.5%、破损率0.1%、含土率7.5%,各项指标优于行业和地方标准规定。     

浙江安吉主要植被类型根系分布特征研究

采用土柱法对黄浦江源区—浙江省安吉县主要植被类型林木细根分布特征进行了研究,结果表明：不同植被类型细根分布有较大差异,茶园、毛竹林、灌木林的根长、根表面积都要明显高于其他几种植被类型,而落阔林的根体积最大,常阔林的根重最大;细根随土层深度的增加而减少,林地细根主要分布在0-40cm土层。草地主要分布在0-30cm土层,这对抵抗径流侵蚀、保持水土资源具有重要意义;各样地根长均以〈0．5mm径级的根长含量最高,0．5-1mm径级次之,而2-5mm径级的最少。根系体积和根重具有相同的分布趋势。大小顺序均为：2—5mm〉1—2mm〉0．5-1mm〉0．5mm,与根长的分布状况正好相反。不同径级根表面积分布差异较大。     

油茶近成熟种子表达的发育相关基因及其分析

以油茶优良无性系湘林1号和湘林4号近成熟种子为材料构建cDNA文库．随机挑取2327个克隆进行3’端测序，并将所得序列与核酸数据库进行同源性比较．发现16种58条与油茶种子胚胎发育及种子成熟相关的基因序列，这些序列与核酸数据库中其他物种相关序列有很高或较高的同源性．其中，脱落酸应答蛋白、成熟控制蛋白、翻译控制肿瘤蛋白、胚胎特化蛋白、乙烯应答因子包含域蛋白等基因序列为高丰度表达；种子成熟蛋白、细胞分化蛋白、乙烯应答因子、脱水素等编码基因属中等丰度表达；而成熟相关蛋白、胚珠发育蛋白、伸展蛋白、生长控制因子、休眠相关蛋白、衰老相关蛋白和脱水应答蛋白等基因只检测到1条序列．属低丰度表达．这些结果为揭示油茶种子油脂转化过程中的基因表达和生理过程提供了科学依据．     

Pharmacokinetics and Metabolism Study of Deep-Sea-Derived Butyrolactone I in Rats by UHPLC–MS/MS and UHPLC–Q-TOF-MS

Butyrolactone I (BTL-I) is a butanolide isolated from the deep-sea-derived fungus, Aspergillus sp. It provides a potential new target for the prevention and treatment of food allergies. This study aimed to investigate the metabolic and pharmacokinetic profile of BTL-I in rats. The metabolic profiles were obtained by UHPLC–Q-TOF-MS. As a result, eleven metabolites were structurally identified, and the proposed metabolic pathways of BTL-I were characterized. The main metabolites were the oxidative and glucuronidative metabolites. In addition, a sensitive UHPLC–MS/MS method was established for the quantitation of BTL-I in rat plasma (LOQ = 2 ng/mL). The method was fully validated and successfully applied to the pharmacokinetic study of BTL-I in rats after oral administration or intravenous administration. The oral bioavailability was calculated as 6.29%, and the maximum plasma concentrations were 9.85 ± 1.54 ng/mL and 17.97 ± 1.36 ng/mL for intravenous and intragastric dosing groups, respectively.     

适于新疆长绒棉的综合机采栽培技术

新疆长绒棉品质优良,在提高我国纺织产品档次、增强行业竞争力方面发挥了重要作用.为了更好地指导新疆长绒棉机采种植,课题组在集成前人对长绒棉优质高效高产栽培技术以及机采棉高效综合栽培技术基础上,结合长绒棉机采种植的特殊要求,提出了机采长绒棉品种要求、机采种植管理技术要点和脱叶采收技术要点,总结形成了适于新疆长绒棉的综合机采...     

不同植物生长促进剂对香蕉假植大苗培育的调控作用

采用盆栽试验,在香蕉假植大苗培育过程中分别喷施浓度均为20 mg/L的赤霉素、复硝酚钠、胺鲜酯、α-萘乙酸钠等植物生长促进剂,以喷施清水为对照,通过不同处理假植大苗生长、物质累积及生理指标的分析,明确不同植物生长促进剂在香蕉假植大苗调控的作用。结果表明,本试验采用的4种植物生长促进剂对香蕉假植大苗培育的调控作用不同。与对照相比,叶面分别喷施20mg/L赤霉素、复硝酚钠均能有利于香蕉假植大苗假茎高及叶面积增加,提高叶绿素含量及叶绿素a、b含量,且以上两种植物生长促进剂平均提高叶绿素含量26.46%;喷施20mg/Lα-萘乙酸钠能提高假植苗假茎粗,提高干物质累积量16.11%积及吸氮量9.95%,促进氮磷钾养分吸收。叶面喷施赤霉素、复硝酚钠、α-萘乙酸钠和胺鲜酯均能显著提高香蕉假植大苗抗性,平均降低丙二醛含量14.64%、提高脯氨酸含量64.36%和SOD含量16.74%,且赤霉素还能显著提高POD含量82.13%。     

早杂新组合T78优2155综合栽培技术措施初探

通过对T78优2155早季栽培施肥水平、施用方法、密植规格的统计分析,结果表明以每667m2施纯N14kg、插植规格20 cm×20 cm,基肥、蘖肥、穗肥比例为5:3:2效果最佳,有利于获得高产。     

Uterine Expression of WNT7A and β-catenin After Induction of Oestrus in Sheep

WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus.