The genetic structure and recombination analyses of Sweetpotato leaf curl virus (SPLCV) population in China

Journal of Plant Diseases and Protection - Sweetpotato leaf curl virus (SPLCV) is a species of virus that causes substantial yield losses in sweetpotato. In this study, twenty-four...     

灵芝的生物学特性、液体发酵及药理作用的研究进展

以灵芝生长特性、液体发酵、药理作用为切入点,综合查询近5年发表的收录于中国知网和艾斯维尔等数据库中的相关文献,就灵芝的生物学特性、液体发酵条件、药理作用方面的研究进展进行综述,为更好地开发利用灵芝资源提供依据。指出现今灵芝的药理作用等基础研究较多,而生长栽培和发酵条件优化的研究报道则较少,而其临床应用的机制则有待进一步探讨。     

Genetic variation of seedling traits responded to brassinosteroid and gibberellin inhibitors in maize (Zea mays) doubled haploid lines

The purpose of this study was to examine the genotypic variation in maize doubled haploid (DH) lines response to brassinosteroid and gibberellin inhibitors. Plant responses to hormone inhibitors were determined in growth chamber experiments using germination paper for three different seedling treatments: application of propiconazole (Pcz), uniconazole (Ucz) or water (control). Mesocotyl length (ML) was more sensitive to hormone inhibitors, especially to the Ucz treatment, than other seedling traits. ML was significantly correlated with other traits in the Ucz treatment. All the seedling traits showed moderate-to-high broad sense heritability values, ranging from 0.39 to 0.82. The Euclidian genetic distances of inbred line pairs ranged from 1.27 to 19.94, indicating there was a high level of variability across the maize DH lines used in this study. DH lines with extreme MLs were identified, which can provide valuable breeding resources for improving abiotic stress tolerance, and for further genetic studies.     

水稻种子总RNA提取质量问题的探究

水稻种子总RNA提取在水稻分子遗传基础实验中起着非常重要的作用,然而,高质量水稻种子RNA的获取难度高,极大阻碍了cDNA文库构建、RNA-Seq和基因表达分析等相关研究工作的开展和深入。为了探讨水稻种子总RNA提取的相关影响因素,寻找合适的水稻种子总RNA提取方式,本研究通过比较Trizol法、GeneMark试剂盒法和艾德莱试剂盒法3种不同的提取方式、不同的耗材处理方法、研磨方式等不同因素改进水稻种子总RNA的提取效果。不同的检测结果显示,艾德莱多糖多酚提取试剂盒能够提取到28S、18S条带完整、降解少和纯度高的水稻种子总RNA;不同耗材处理方法中,蛋白酶K处理过的耗材提取的总RNA比另外两种方法处理过的耗材提取的总RNA降解更少,28S和18S条带更清晰和完整、总RNA质量最高、效果最好;而研磨方式上,液氮冷冻研磨法能够有效抑制RNA酶对RNA的降解。本研究对水稻种子RNA提取过程相关因素的分析和改进,为促进以水稻种子为研究对象的分子实验奠定了一定的技术基础。     

盐酸返滴定法测定玉米脂肪酸值的可行性

为验证玉米脂肪酸值快速、简便、直观、准确的测定方法,以现行的国家标准GB/T 20570-2015《玉米储存品质判定规则》附录A中手工滴定法和电位滴定法为基础,采用盐酸返滴定法,对外观颜色相近的28个玉米样品及外观颜色明显差异的11个不同玉米品种的脂肪酸值进行测定。结果表明:28个外观颜色相近的玉米样品脂肪酸值手工滴定法与盐酸返滴定法的最小差为0.06 mg/100g,最大差为1.98mg/100g(小于2 mg/100g),2种方法测定脂肪酸值结果基本一致;二者的标准差分别在0.03～0.95mg/100g和0.11～0.82 mg/100g,RSD分别在0.22%～4.47%和0.62%～3.16%(RSD均小于5%),方法稳定性好。外观颜色明显差异的11个玉米品种脂肪酸测定值在手工滴定、电位滴定和盐酸返滴定3种方法间存在显著线性相关性(R2≥0.993)。盐酸返滴定法测定玉米脂肪酸值其精密度和稳定性均能满足测定要求,滴定速度快,终点颜色易判断,测定结果准确度高。     

Uterine Expression of WNT7A and β-catenin After Induction of Oestrus in Sheep

WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus.     

晚春造林技术研究

通过简单易行而有效的技术措施,成功试验且规模推广晚春造林技术,解决了当地春季火烧迹地更新造林季节脱节的矛盾。     

水丰水库渔获变化和鱼类资源现状及小型经济鱼虾种间关系分析

水丰水库的渔获量1961－1980年间变化于47－300t,1981-1984年增长到740－1321t,1985-2000年为2270－4926t。渔获物组成中以亚洲公鱼为主，1980－2000年亚洲蚣鱼占总渔获的29．6％－93．0％，平均为65．6％。鲁鲫鱼占6．2％－16．4％，鲢鳙鱼占8．1％－13．3％，鳊鲂占1．8％－11．3％，虾占5．9％－16．1％，其它鱼类占2．4％－9．3％。2000年大银鱼占总渔获的2．8％。1985－2000年水库鱼类资源相对稳定。亚洲公鱼、青虾和大银鱼三种小型经济鱼虾的种间关系表现为互利共生，竞争和残敌关系，可能向种间形成平衡调节方向发展。     

Effect of Chitosan on Intestinal Bacteria of Allogynogenetic Crucian Carp,Carassius auratus gibelio,as Depicted by polymerase chain reaction‐denaturing Gradient Gel Electrophoresis

A 16S rDNA‐based polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE) method was applied to detect intestinal bacterial communities of juvenile allogynogenetic crucian carp, Carassius auratus gibelio, fed with chitosan‐containing diet. This is the first time to use the molecular method to analyze the bacterial communities in the allogynogenetic crucian carp intestine. The DGGE profile with universal bacterial primers revealed simple communities in all treatment groups. Sequencing and phylogenetic analysis of excised DGGE bands showed that the dominant bacteria belonged to class γ‐Proteobacteria and Fusobacteria. The relative abundance and diversity of detected bacteria suggested that 0.5 and 0.75% of chitosan in diet were optimum for juvenile allogynogenetic crucian carp. As in these concentrations, some detected pathogen bacteria either disappeared or decreased. However, the DGGE profile with Aeromonas‐specific primers showed a similar composition among all treatment groups, which suggested that Aeromonas was one of relative stable bacteria components in the intestine of juvenile allogynogenetic crucian carp.