江西地区鸭疫里氏杆菌的鉴定及药敏试验

从江西部分地区的25份临床疑似鸭疫里氏杆菌(RA)病例中分离到19株RA。生化特性测定结果表明,19个RA菌株的过氧化氢酶、尿素酶均为阳性,除1株发酵葡萄糖、1株发酵麦芽糖外均不发酵各种糖类,5个RA菌株能液化明胶,15个RA菌株氧化酶阳性,不产生H2S,不还原硝酸盐,吲哚试验、MR试验、VP试验均阴性;80%的菌株对头孢曲松、头孢哌酮、头孢唑啉、罗红霉素等高度敏感,而对阿米卡星、庆大霉素、复方新诺明、卡那霉素、青霉素等耐药。     

Pharmacokinetics of mequindox and one of its major metabolites in chickens after intravenous, intramuscular and oral administration

Pharmacokinetics of mequindox and one of its major metabolites (M) was determined in chickens after intravenous (i.v.), intramuscular (i.m.) and oral administration of mequindox at a single dose of 10 (i.v. and i.m.) or 20 mg/kg b.w. (oral). Plasma concentration profiles were analyzed by a non-compartmental pharmacokinetic method. Following i.v., i.m. and oral administration, the areas under the plasma concentration-time curve (AUC(0-∞)) were 0.71±0.15, 0.67±0.21, 0.25±0.10 μg h/mL (mequindox) and 37.24±7.98, 36.40±9.16, 86.39±16.01 μg h/mL (M), respectively. The terminal elimination half-lives (t(1/2λz)) were determined to be 0.15±0.06, 0.21±0.09, 0.49±0.23 h (mequindox) and 5.36±0.86, 5.39±0.52, 5.22±0.35 h (M), respectively. The bioavailabilities (F) of mequindox were 89.4% and 16.6% for i.m. and oral administration. Steady-state distribution volume (V(ss)) of 1.20±0.34 L/kg and total body clearance (Cl(B)) of 13.57±2.16 L/kg h were determined for mequindox after i.v. dosing. After single i.m. and oral administration, peak plasma concentrations (C(max)) of 3.04±1.32, 0.36±0.13 μg/mL (mequindox) and 3.81±0.92, 5.99±1.16 μg/mL (M) were observed at t(max) of 0.08±0.02, 0.32±0.12 h (mequindox) and 0.66±0.19, 6.67±1.03 h (M), respectively. The results showed that mequindox was rapidly absorbed after i.m. or p.o. administration and most of mequindox was transformed to metabolites in chickens, with much higher C(max)s and AUCs of metabolite (M) than those of mequindox in plasma.     

Molecular taxonomic relationships of Psoroptes and Chorioptes mites from China based on COI and 18S rDNA gene sequences

In this present study, the mitochondrial DNA gene cytochrome coxidase subunit I (COI) and the small subunit ribosomal RNA (18S rDNA) gene were used to determine the taxonomic relationships of Psoroptes and Chorioptes mites from China. The neighbor-joining and maximum-parasimony approach were used to evaluate the evolutionary relatedness among different hosts in the genera Psoroptes and Chorioptes. Phylogenetic analysis showed that Psoroptes cuniculi and Psoroptes natalensis may be two different species within the genus Psoroptes, and Chorioptes texanus and Chorioptes panda are different species within the genus Chorioptes.     

饲料企业制造成本系统控制模型

企业的竞争力来源于两个因素：一是品质．二是成本。通过精益生产．减少浪费．是最行之有效的控制成本的方法之一。制造成本是决定饲料企业经济效益和市场竞争力的一个重要因素．随着原料价格的不断攀升,企业的利润越来越低．降低生产成本．提高生产效率成为饲料企业的利润增长点。但在目前饲料行业中．中小型饲料厂比较多,固定资产投资比较小,现代化生产设备比较少,     

浅析配方对饲料生产制程的作用与影响

在医学领域,有这样一个奇怪的现象：同样的药方．为什么在古代具有奇效．而在现代却屡屡失效？在饲料企业．也有同样的怪现象,同样的配方,生产出来的产品却不一样!     

Molecular cloning,chromosomal localization and expression pattern of porcine ADP‐ribosylation factor(Arf) gene family

Adenosine diphosphate‐ribosylation factors (Arfs) are a family of guanosine triphosphate‐binding proteins involved in fundamental biological processes including secretion, endocytosis, phagocytosis, cytokinesis, cell adhesion and tumor cell invasion. We report here the molecular cloning, chromosome localization and expression analysis of porcine Arf1–6, of which Arf1–3 (Class I) have >93% similarity to each other and encode nearly similar proteins with 181 amino acids in length. Arf4 and Arf5 (Class II) are 78–81% homologous to Class I Arfs and both encode a protein of 180 amino acids, Arf6 (Class III) shows 64–68% homology to the other Arfs and encodes 175 amino acids. With radiation hybrid mapping, porcine Arf1–6 are assigned to chromosomes 14q21‐q22, 12p14, 5p12‐q11, 13q21.1, 18q24, 1q21‐q27, respectively. Moreover, real‐time quantitative RT‐PCR assays show that porcine Arf1‐6 are ubiquitous in all tissues examined, with the highest levels in the kidney and stomach and the lowest in muscle and the heart. This is the first report of molecular characterization of the Arf gene family in pigs.     

盐胁迫对星星草幼苗保护酶系统的影响

在盐胁迫下,星星草幼苗过氧化氢酶活性随着胁迫时间的延长而增强,并与盐浓度呈显著正相关。超氧化物歧化酶活性随着胁迫浓度的增强而增强,当超过一定浓度(3.0%Na₂CO₃)时则趋减弱,尔后随着胁迫时间的延长而增强。过氧化物酶活性的变化规律与超氧化物歧化酶活性相似,但变化幅度较小。丙二醛含量随着胁迫时间的延长和胁迫强度的增强而增加。     

松嫩平原碱化草甸朝鲜碱茅种群生殖特性的定量分析

通过对朝鲜碱茅单化群落独立株丛的大样本取样和对正常花序随机取样的调查与测定，定量分析了松嫩平原碱化草甸朝鲜碱茅种群的生殖特性。朝鲜碱茅种群话营养繁殖数量性状之间有较强的规律性。丛分蘖数、丛地上生物量、丛生殖枝数和丛生殖技重分别与丛径之间，以及丛生殖枝重与丛生殖枝数之间均为线性正相关，丛生物量随着丛分蘖数的增加呈幂函数增加。朝鲜碱茅种群在整个生殖生长发育过程中具有较强的有序性。圆锥花序各节位上的小穗数量呈伽玛分布。各节位上的小花数量随着节位的增高呈Logistic曲线形式减少。小穗数和小花数均随着节位的增加呈y＝ae(－b／x)饱和型指数曲线形式累积增长。     

通过器官病变诊断鸭病

一发病情况本县一养鹅专业户葛某饲养有220只浙东大白鹅的种鹅,2009年1月8日从邻近乡一鹅场购入苗鹅600只。购入一周后用禽流感疫苗注射0．5mL,     

对处理一起市场上投诉的黄膘猪肉的体会

1事件回顾
　　2010年7月某日,某市某农贸市场有肉商向市场管理部门投诉,称其雇人在该市某县屠宰厂屠宰的猪为黄膘猪,请市场管理部门协助处理,并把猪胴体、内脏丢弃在市场管理办公室前的地上,引来群众围观。市场管理方当即向辖区的城区政府汇报;也有群众分别致电当地媒体和市政府热线,政府热线要求市水产畜牧兽医局处理,市水产畜牧兽医局安排市动物卫生监督所派员处理。