网络环境下生物信息资源的检索

本文从生物信息学的理论出发，讨论了如何通过互联网检索各种生物信息资源，最大限度地利用互联网为生物科学的教学科研服务。     

菜豆萎蔫病菌的PCR检测技术

根据在GenBank已登录的萎蔫短小杆菌(Curtobacterium flaccumfaciens)的基因间重复序列，用DNAman5．0软件比较同源性，设计了一对引物CffF1和CffR2，并以菜豆萎蔫病菌的基因组DNA为模板进行扩增，得到了一段长280bp的PCR特异产物。参试的其他属如棒形细菌属(Clavibacter)、节杆菌属(Arthrobacter)、拉氏杆菌属(Rathayibacter)、红球菌属(Rhodococcus)细菌和萎蔫短小杆菌的其他致病变种(Cur．f.pv．oortii、Cur.f.pv．poinsettiae、Cur．f.pv．betae)，以及其他植物病原细菌的基因组DNA均无扩增产物。该检测方法特异性强、灵敏度高，在100Pg的基因组DNA浓度下仍能检测到很强的条带。     

1-没食子酸甘油酯的研制及其抗氧化性能的研究

在实验室条件下,以没食子酸和甘油为原料合成了1-没食子酸甘油酯(GG),做为油脂或油脂食品的抗氧化剂,保护油脂不因氧化腐败,延长贮存时间。合成途径是在日本高砂光正等人工作基础上进行了若干改进,使得率由45.7％提高到72.9％。以菜子油和豆油做底物,丙酯(PG)和2,6-二特丁基对甲酚(BHT)做对比抗氧化剂,用增重法评价其抗氧化性能,柠檬酸和酒石酸做增效剂,评价其增效作用,研究了浓度和分散度对诱导期的影响,探讨了结构因素对性能的影响。实验室结果表明:在本实验条件下,活性次序是GG>PG>BHT,随着浓度的增加,活性增大顺序是GG>PG>BHT。酒石酸对GG有增效作用,柠檬酸无效。溶解度对诱导期没有影响,说明了性能差异产生的原因。通过研究为简化合成途径,进行工业化生产提供了依据,并在此基础上为合成新型抗氧化剂找到一种可行方法。     

串番茄新品种红星302 的选育

红星302 是以抗病、耐低温弱光材料01-61 为母本,以01-23 为父本育成的串番茄一代杂种,无限生长类型,果实串收,果色亮红,果实椭圆形,酸甜适口,品质佳,VC 含量为364 mg · kg^-1,可滴定酸0.44%,可溶性糖4.25%,平均单果质量30.79 g,硬度为11.40×10⁵ Pa。单株结果数约为53个,产量4 900 kg ·（ 667 m²）^-1 左右,高抗蕨叶病毒病、青枯病,适宜河北地区冬季日光温室和早春保护地栽培。     

三水雪梨瓜嫁接育苗技术

为了解决三水区雪梨瓜种植面积的不断扩大、复种指数不断提高,品质退化、产量降低、病虫害严重的现象越来越突出的问题,佛山市农业科学研究所申请并开展了"蔬菜嫁接栽培技术研究及示范推广"课题研究,从2013—2015年,摸索出了一套适合本地生产雪梨瓜嫁接苗的技术和方法,即通过选择砧木、选择播期、营养土配方、高效嫁接、苗期管理、成苗运输等措施,缓解了嫁接操作不规范、生产管理不统一、苗木质量差异大、产量品质不理想的难题,初步实现雪梨瓜嫁接苗的工厂化、规模化生产,为三水雪梨瓜种植业的可持续发展提供了有力保障。     

Screening of lentiviral vectors carrying effective siRNA targeting S1P2 gene

AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×10⁵ cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency.     

Effects of freeze-thaw cycles and initial soil moisture content on soil aggregate stability in natural grassland and Chinese pine forest on the Loess Plateau of China

Journal of Soils and Sediments - Ongoing global warming is decreasing the thickness of snow covers and increasing initial soil moisture content (SMC), which increase the number of freeze-thaw...     

信阳市芽苗菜发展前景分析

结合信阳市独特的饮食文化以及芽苗菜的特点、生产技术等,分析了信阳发展芽苗菜生产的途径。     

黄颡鱼毒棘结构研究

黄颡鱼（Pelteobagrus fulvidraco）是较为典型的淡水刺毒鱼类,本研究结合宏观毒棘骨骼标本制作和微观组织切片等方法,深入探究黄颡鱼的毒棘结构。研究结果表明,黄颡鱼的胸鳍和背鳍均具有尖锐的倒刺棘骨,棘骨外包围着皮膜,皮膜拥有能分泌毒液的毒腺细胞,它们共同组成了黄颡鱼毒棘装置。毒棘骨骼宏观结构表明,黄颡鱼的背鳍和胸鳍毒棘均具有贯穿全棘骨的中央管和包围棘骨的皮膜;背鳍棘骨细长、锥形,且轻微拱起,顶部骤尖,后缘为弱锯齿;胸鳍棘骨前后缘均有锯齿,但后缘为强锯齿。背鳍和胸鳍毒棘基部结构的髁突形状、大小、位置全然不同。微观结构显示,皮膜中的毒腺细胞聚集成层,位于鳞状上皮与色素层之间,未见导管与之相连,背鳍和胸鳍毒棘的分支性骨管中均未发现毒腺细胞。胸鳍和背鳍锁紧装置可让毒棘保持倒伏或者直立的锁定状态,降低猎物挣脱几率。本研究可深入了解黄颡鱼的毒棘结构和特征,增强人们对刺毒鱼类的认知,同时为淡水刺毒鱼类深入研究奠定基础。