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91.
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Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep 下载免费PDF全文
J. Wang H. Zhou J. Hu S. Li Y. Luo J.G.H. Hickford 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2016,133(3):219-226
Myostatin is a negative regulator of muscle growth and development in mammals, and variation in ovine myostatin gene (MSTN) has been demonstrated to be associated with variation in the muscularity of sheep. Polymerase chain reaction–single‐stranded conformational polymorphism (PCR–SSCP) was used to look for single nucleotide polymorphisms (SNPs) in a 304‐bp amplicon from the promoter region of ovine MSTN. Sequence analyses revealed two previously identified SNPs (c.?2449G/C and c. ?2379T/C) that resulted in three haplotypes (H1 (c.[?2449G; ?2379C]), H2 (c.[?2449C; ?2379C]) and H3 (c.[?2449G; ?2379T]). The effect of these SNPs on growth and carcass traits was investigated in 357 NZ Romney lambs. General linear mixed‐effect models revealed that sheep with the genotype c.?2449GC had a higher loin meat yield (p = 0.032) and proportion loin yield (p = 0.028), than those with the genotype c.?2449GG. The genotype c.?2379CC was associated with an increase in three weight traits: birthweight (p = 0.003), tailing weight (p = 0.009) and weaning weight (p = 0.028), when compared with the genotype c.?2379TC, but it was not found to have an association with growth rate. This suggests that c.?2379T/C has an effect that originates at, or before birth. Haplotype H3 was associated with a decrease in birthweight (p = 0.002), tailing weight (p = 0.003) and weaning weight (p = 0.011). Haplotype H2 was associated with increased loin yield (p = 0.012) and proportion loin yield (p = 0.002). The SNPs may have value as genetic markers for improved Romney breeding. 相似文献
93.
Licht BG Lin S Luo Y Hyson LL Licht MH Harper KM Sullivan SA Fernandez SA Johnston EV 《Journal of the American Veterinary Medical Association》2007,231(10):1520-1528
OBJECTIVE: To determine clinical characteristics and mode of inheritance of seizures in a family of Standard Poodles. DESIGN: Case series. ANIMALS: 90 Standard Poodles descended from the same maternal bloodline (30 with probable idiopathic epilepsy [PIE] and 60 without any history of seizures). PROCEDURES: Researchers contacted owners to determine whether dogs had ever had any seizures and, if so, the nature of any such seizures and any potential underlying causes. Dogs were considered to have PIE if they were between 6 months and 7.5 years old at the time of seizure onset and had no evidence of any underlying cause. To determine the mode of inheritance, segregation analyses were designed to allow the family to be analyzed as a whole, as opposed to as nuclear families. Competing models of inheritance were compared statistically for their ability to explain the data. RESULTS: Of the dogs with PIE, 28 (93%) had focal onset seizures with or without secondary generalization. Median age of onset was 3.7 years; 6 dogs were > 5 years old at the onset of seizures. Segregation analyses strongly suggested that PIE was inherited as a simple recessive autosomal trait with complete or almost complete penetrance. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that in this family of Standard Poodles, PIE was inherited as a simple recessive autosomal trait with complete or almost complete penetrance. Seizures often had focal, as opposed to generalized, onsets, and it was not uncommon for seizures to begin after 5 years of age. 相似文献
94.
Ghrelin, acts as the endogenous ligand for growth hormone secretagogues receptor (GHS-R), is a novel growth hormone (GH) releasing peptide with reported effects on food intake in chickens. In this study, an 8 bp indel polymorphism in exon 1 of the chicken Ghrelin (cGHRL) gene was genotyped in a F(2) designed full-sib population to analyze its associations with chicken growth and carcass traits. Later, mRNA level in the proventriculus was determined by real-time PCR to reveal the expression feature of cGHRL gene. Result showed that this 8 bp indel was significantly associated with body weight at the age of 28 days (BW28) and 56 days (BW56), eviscerated weight (EW) and leg muscle weight (LMW) (P<0.05), highly significantly associated with hatch weight (HW), BW14, 21, 35, 42, 49, 90 and body length (BL), dressed weight (DW), eviscerated weight with giblet (EWG), wing weight (WW), breast muscle weight (BMW) and head and neck weight (HNW) (P<0.01). Meanwhile, A allele (with 'CTAACCTG') was positive for chicken growth as individuals with AA genotype had the highest value of all traits. Analysis on cGhrelin mRNA level revealed that it differed significantly among individuals with three genotypes (P<0.05). Individuals with AB genotype had the highest mRNA level, whereas that of AA had the lowest one. It was concluded that this 8 bp indel of cGHRL gene was significantly associated with most body weight and body composition traits, and negative effect of endogenous Ghrelin on chicken growth were indicated by this study. 相似文献
95.
本试验旨在研究不同铜源(硫酸铜、碱式氯化铜和柠檬酸铜)和铜添加水平(20和30 mg/kg)对断奶仔猪生长性能、血清铜含量与含铜酶活性以及组织铜含量的影响,探讨断奶仔猪对不同铜源的相对生物学利用率。试验采用3×2双因子随机区组设计,选取平均体重为(8.98±0.48) kg的杜×长×大三元杂交断奶仔猪112头,随机分为7组,每组4个重复,每个重复4头猪。对照组饲喂玉米-豆粕型基础饲粮(铜含量为7.80 mg/kg),各试验组分别在基础饲粮中添加20或30 mg/kg硫酸铜、碱式氯化铜或柠檬酸铜(均以铜含量计)。试验预试期3 d,正试期28 d。结果表明:1)试验组与对照组断奶仔猪平均日增重、平均日采食量和料重比均无显著差异(P>0.05)。2)饲粮铜源和铜添加水平对断奶仔猪血清铜含量具有显著影响(P<0.05),但对血清铜蓝蛋白和铜锌超氧化物歧化酶活性均无显著影响(P>0.05)。3)饲粮铜源和铜添加水平对断奶仔猪肝脏铜含量具有显著影响(P<0.05),但对心脏、肾脏、胰脏和跖骨铜含量均无显著影响(P>0.05)。4)根据多元线性回归斜率比法计算,以血清铜... 相似文献
96.
97.
98.
红酵母的性质及其应用研究 总被引:10,自引:0,他引:10
红酵母是酵母菌的一个属,腐生,分布广泛,在医学、食品、化工和农业等方面都有一定的应用,但目前并没有引起人们足够的重视。综述了红酵母的种属特性、理化特性、培养及其应用。 相似文献
99.
种子企业要在种子竞争中立于不败之地,就必须积极加快科研开发的步伐,全面启动品种创新,培育出更有竞争力的农作物新品种,才能使企业步入健康发展的轨道。同时对获得的植物新品种还应该认真加强保护,这样既保障了企业的应有权益,也激励了农业技术持续创新,规范了市场经济秩序,从而发挥知识产权为企业带来的经济效益,促使企业的科技进步。 相似文献
100.
Based on the data of dynamic tests of small single-storey steel frames,the suitability of simplified hi-linear restoring force model in earthquake response analysis is discussed, and the Maximum displacement response MD, the sum of squares of the displacements in the whole vibrating history SSD, and the total dissipated energy EI are considered respectively as equivalent object. 相似文献