全文获取类型
收费全文 | 22297篇 |
免费 | 336篇 |
国内免费 | 650篇 |
专业分类
林业 | 4289篇 |
农学 | 2149篇 |
基础科学 | 604篇 |
3514篇 | |
综合类 | 2802篇 |
农作物 | 2487篇 |
水产渔业 | 2000篇 |
畜牧兽医 | 1769篇 |
园艺 | 1387篇 |
植物保护 | 2282篇 |
出版年
2024年 | 15篇 |
2023年 | 67篇 |
2022年 | 210篇 |
2021年 | 220篇 |
2020年 | 247篇 |
2019年 | 195篇 |
2018年 | 2874篇 |
2017年 | 2912篇 |
2016年 | 1369篇 |
2015年 | 303篇 |
2014年 | 275篇 |
2013年 | 347篇 |
2012年 | 1200篇 |
2011年 | 2537篇 |
2010年 | 2434篇 |
2009年 | 1588篇 |
2008年 | 1660篇 |
2007年 | 1897篇 |
2006年 | 316篇 |
2005年 | 346篇 |
2004年 | 212篇 |
2003年 | 347篇 |
2002年 | 324篇 |
2001年 | 221篇 |
2000年 | 194篇 |
1999年 | 152篇 |
1998年 | 101篇 |
1997年 | 77篇 |
1996年 | 82篇 |
1995年 | 82篇 |
1994年 | 72篇 |
1993年 | 85篇 |
1992年 | 61篇 |
1991年 | 48篇 |
1990年 | 54篇 |
1989年 | 35篇 |
1988年 | 38篇 |
1987年 | 23篇 |
1986年 | 13篇 |
1985年 | 5篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1977年 | 4篇 |
1969年 | 1篇 |
1968年 | 4篇 |
1967年 | 1篇 |
1956年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
犬Ⅱ型腺病毒自然弱毒株的分离与鉴定 总被引:6,自引:0,他引:6
从一只3月龄病犬中分离出一株犬腺病毒(CAV),暂定名为YCA18。从一系列形态学、生理化学、生物学和分子病毒学实验证明这是一株Ⅱ型犬腺病毒自然弱毒。此毒株能在犬、猪肾原代细胞和传代细胞系上生长,能产生腺病毒感染的特征性细胞病变,细胞肿胀,变圆和呈“葡萄串样”。在细胞核内可见病毒粒子及其前本的晶格状排列,病毒粒子呈20面体立体对称,表面有排列整齐的壳粒;能抵抗乙醚的处理,在PH3及50℃温度中稳定 相似文献
22.
Wang Y Bai Y Qu Q Xu J Chen Y Zhong Z Qiu Y Wang T Du X Wang Z Yu S Fu S Yuan J Zhen Q Yu Y Chen Z Huang L 《Veterinary microbiology》2011,151(3-4):354-362
Brucellosis brings great economic burdens for developing countries. Live attenuated vaccines are the most efficient means for prevention and control of animal Brucellosis. However, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. Therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. VjbR is a quorum sensing regulator involving in Brucella's intracellular survival. The vjbR∷Tn5 mutants have been proven effective against wild type strain challenge, implying its possibility of use in vaccine candidate development. To further evaluate this candidate gene, in the present study, the antigenicity of purified recombinant VjbR protein was analyzed. Antibodies to Brucella melitensis VjbR could be detected in sera from patients and animals with brucellosis but not in control ones, implying the potential use of this protein as a diagnostic antigen. Then a vjbR mutant of B. melitensis 16M was constructed by replacing the vjbR with kanamycin gene. The mutant showed reduced survival in macrophage and mice. Vaccination of BALB/c mice with 16MΔvjbR conferred significant protective immunity against B. melitensis strain 16M challenges, being equivalent to which induced by the license vaccine Rev.1. The vjbR deletion mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon and interleukin-10. The most importance is that, the use of vjbR mutants as vaccines in association with diagnostic tests based on the VjbR antigen would allow the serological differentiation between infected and vaccinated animals. These results suggest that 16MΔvjbR is an ideal live attenuated vaccine candidate against B. melitensis and deserves further evaluation for vaccine development. 相似文献
23.
分析了西部地区肉牛产业化发展的限制因素,提出了加速我国西部地区肉牛产业化发展的对策与思路,推行品种良种化、技术先进化、管理企业化的生产模式,促进肉牛生产向优质、高产、高效和持续性的产业化方向发展。 相似文献
24.
针对使用搅刀-拨轮式排肥器的悬挂施肥播种机在施尿素等颗粒状肥料时,非工作状态所发生的肥料严重漏失问题,研究设计了阻止漏肥装置。试验结果表明,该装置利用播种机被提升和落下动作即能自动控制挡肥活门启闭,不增加拖拉机驾驶员的操作负担,能有效解决上述排肥器的漏肥问题。 相似文献
25.
Influence of Ammoniacal Copper Quaternary treatments on mechanical properties of blue-stained Lodgepole Pine wood 总被引:1,自引:0,他引:1
Three concentrations (2.8%, 2.0%, 1.2%) of Ammoniacal Copper Quaternary (ACQ) was selected to treat Lodgepole pine wood for evaluating ACQ treatment on mechanical properties of blue-stained wood. The bending modules of elasticity (MOE), modules of rupture (MOR), toughness and shearing strength parallel to grain on tangential surface, are tested according to the criteria GB1927-1943-91. Non-treated sample were also tested according to the same procedure. The results showed that the three groups specimen impregnated by different concentrations of ACQ solution met the AWPA standard 2003 of America (UC4A 6.4g/cm^3). There were significant difference of toughness between treated wood and non-treated wood (p=0.01), but there were no statistically significant differences among three concentrations in terms of toughness, and toughness of treated wood was approximately 20% lower than non-treated. MOR, MOE as well as sheafing strength parallel to grain were found to be not significantly different between treated wood and non-treated one, and there were no statistically significant difference among three concentrations of ACQ too. Toughness, MOR, MOE and sheafing strength parallel to grain increased with decrease of concentration of ACQ, but they were hardly affected by ACQ preservatives. 相似文献
26.
Deforestation and degradation of productive lands are serious threats to the sustainability of forestry/agricultural practices
in Kenya. In the last two decades farm forestry (FF) has been promoted through pilot projects among local communities as an
example of sustainable land use. However adoption of FF is limited outside the project locations because FF improvement measures
focused mainly on biological (e.g. succession, biodiversity and traditional industrial timber production) and technical concerns
(e.g. material input delivery such as providing free tree seedlings for field planting) rather than local values, and interests
and the constraints facing farmers. This study examined the local farm priorities and constraints and the prospects for the
wider implementation of farm-level tree planting in four communities in Rachuonyo District. Using interviews with 597 randomly
selected household heads, the study assessed farmer’s production assets and activities, land tenure, priority tree species
and the constraints to growing trees on farms. Results show that farm labour is represented by a young population, 56.3% under
the age of forty. They are mainly engaged in small-scale mixed cropping integrated with multipurpose trees and some livestock.
Tree products contribute about 32% to household cash income, more than any other source (agricultural products, labour sales,
etc). Females were more often household heads and had considerable influence over productive activities, making them an important
target group in FF development. Farmers preferred exotic tree species due to their ability to provide short-term cash income,
fuel and shade. Farmers’ concerns included population pressure on limited farmlands and the problem of credit for agricultural
inputs. Given the feeling of secured tenure arrangement and influence of tree products on the household economy, farmers are
likely to invest more in efficient land uses such as FF if consideration is given to local priorities. 相似文献
27.
Syed Jawad Ahmad Shah Shaukat Hussain Musharaf Ahmad Farhatullah Iftikhar Ali Muhammad Ibrahim 《Journal of General Plant Pathology》2011,77(3):174-177
Fifty wheat varieties along with Jupateco-73 and Morocco were studied for the expression of leaf tip necrosis (LTN), a trait
linked with the durable rust resistance gene pair Lr34/Yr18. LTN was frequent (i.e., ≥6) in nine replications of a field experiment over 3 years in 17 genotypes, and the varieties were
considered positive for LTN. In molecular analyses of these varieties, having relative severity values up to 78 for yellow
rust and 45 for leaf rust, the 150-bp Lr34/Yr18-linked allele was consistently amplified. Expression of LTN in six of nine replications is an appropriate threshold for predicting
the presence of Lr34/Yr18 gene pair, and genotypes can be selected using this trait. 相似文献
28.
Fang Xiao Wei Han Yongxiu Li Yixuan Qiu Qing Sun 《The Journal of Horticultural Science and Biotechnology》2018,93(3):306-315
To study the effects of day and night temperature difference (DIF) on tomato growth, a controlled experiment using Solanum lycopersicum L., cv. Jinguan 5 was conducted. The daily mean temperature (Tm) was maintained at 18°C and 25°C, and the DIF was set at 0°C, 6°C, and 12°C. The results indicated that chlorophyll a (Chl a) and chlorophyll b (Chl b) gradually increased as DIF rose. At 18°C Tm, the carotenoid content reached a maximum at 12°C DIF. The Chl a/Chl b, net photosynthetic rate (PN), photosynthetic rate at irradiation saturation (Pmax), stomatal conductance (gs), intercellular CO2 concentration (Ci), stomatal limitation value, the maximum assimilation rate (Amax), apparent quantum efficiency (Aq), carboxylation efficiency (Ce), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), gibberellin A3 (GA3), and indole-3-acetic acid (IAA) were highest, while malondialdehyde (MDA) was lowest at 6°C DIF. At 25°C Tm, PN, Pmax, Amax, Aq, Ce, gs, Ci, CAT, POD, GA3, IAA, and zeatin reached the maximum under 6°C DIF, while SOD and MDA reached the maximum under 12°C DIF. Furthermore, the morphological index peaked at 6°C DIF under 18°C and 25°C Tm. The results suggested that 6°C DIF improved the growth and development of tomato during the flowering stage. ABBREVIATIONS: Aq – apparent quantum efficiency; Amax – the maximum assimilation rate; CAT – Catalase; Chl a(b) – chlorophyll a(b); Ca – ambient CO2 concentration; Ce – carboxylation efficiency; Ci – intercellular CO2 concentration; DIF – difference between day temperature (TD) and night temperature(TN); FM – fresh mass; gs – stomatal conductance; GA3 – gibberellin A3; IAA – indole-3-acetic acid; Ls – stomatal limitation value; MDA – malondialdehyde; Pmax – photosynthetic rate at irradiation saturation; PN – net photosynthetic rate; POD – peroxidase; ROS – reactive oxygen species; SOD – superoxidedismutase; Tm – daily mean temperature; ZT – zeatin. 相似文献
29.
Masaki Kiryu Miho Hamanaka Kayo Yoshitomi Susumu Mochizuki Kazuya Akimitsu Kenji Gomi 《Journal of General Plant Pathology》2018,84(3):221-229
Plant volatile compounds, including terpenes, are known to be involved in the rice defense system. In the present analysis of a terpene synthase, OsTPS18, in rice, we found that OsTPS18 was localized in the cytoplasm and synthesized the sesquiterpenes (E)-nerolidol and (E)-β-farnesene. The amounts of (E)-nerolidol and (E)-β-farnesene increased after jasmonic acid (JA) treatment. (E)-Nerolidol had significant antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo). These results suggest that (E)-nerolidol plays an important role in JA-induced resistance against Xoo and that it functions as an antibacterial compound in rice. 相似文献
30.