Effect of Se and VE Supplement on Semen Quality,Antioxidant Enzyme Activities and Heat Shock Protein Expression of Goat in High Temperature Season

This experiment was conducted to study the effect of selenium and vitamin E supplement on semen quality, antioxidant enzyme activities and heat shock protein expression of goat in Hainan high temperature season.16 adult Hainan Black goat with good health and approximate weight were randomly divided into 4 groups, fed with basal diet(control group), basal diet+0.5 mg/kg Se(Se group), basal diet+100 mg/kg VE(VE group), and basal diet+0.5 mg/kg Se+100 mg/kg VE(Se+VE group), respectively.The experimental period was 93 d.Semen samples were collected in the last week of the experiment on two consecutive days.The semen quality, antioxidant enzyme activities and heat shock protein expression were analyzed.The results showed that compared with control group, the ejaculate volume was not significantly affected by Se or VE supplement(P>0.05).Sperm density and sperm motility were increased significantly by Se and VE supplement(P<0.05), and the abnormal rate was decreased extremely significantly(P<0.01).The goats fed with Se and VE also had higher activities of GSH-Px(P<0.01), SOD(P<0.05), CAT(P<0.05) and T-AOC(P<0.01), and lower MDA concentration in seminal plasma(P<0.05).The relative expression levels of HSP70 and HSP90 mRNA in supplement groups were decreased extremely significantly(P<0.01).However, there were some certain differences between the Se and VE supplement groups on semen quality and heat shock protein expression.In conclusion, the supplementation of Se and VE could help to improve goat semen quality by increasing the sperm density, sperm motility, the antioxidant enzyme activities, and decreasing the abnormal rate in hot season of Hainan.Finally, Se and VE supplement had good effects on relieving the environment heat stress.     

Comparison of the rebound tonometer (TonoVet) with the applanation tonometer (TonoPen XL) in normal Eurasian Eagle owls (Bubo bubo)

OBJECTIVE: To examine the feasibility and accuracy of a handheld rebound tonometer, TonoVet, and to compare the intraocular pressure (IOP) readings of the TonoVet with those of an applanation tonometer, TonoPen XL, in normal Eurasian Eagle owls. ANIMALS STUDIED: Ten clinically normal Eurasian Eagle owls (20 eyes). PROCEDURES: Complete ocular examinations, using slit-lamp biomicroscopy and indirect ophthalmoscopy, were conducted on each raptor. The IOP was measured bilaterally using a rebound tonometer followed by a topical anesthetic agent after 1 min. The TonoPen XL tonometer was applied in both eyes 30 s following topical anesthesia. RESULTS: The mean +/- SD IOP obtained by rebound tonometer was 10.45 +/- 1.64 mmHg (range 7-14 mmHg), and by applanation tonometer was 9.35 +/- 1.81 mmHg (range 6-12 mmHg). There was a significant difference (P = 0.001) in the IOP obtained from both tonometers. The linear regression equation describing the relationship between both devices was y = 0.669x + 4.194 (x = TonoPen XL and y = TonoVet). The determination coefficient (r(2)) was r(2) = 0.550. CONCLUSIONS: The results suggest that readings from the rebound tonometer significantly overestimated those from the applanation tonometer and that the rebound tonometer was tolerated well because of the rapid and minimal stress-inducing method of tonometry in the Eurasian Eagle owls, even without topical anesthesia. Further studies comparing TonoVet with manometric measurements may be necessary to employ rebound tonometer for routine clinical use in Eurasian Eagle owls.     

PTGS/ RNAi的作用机制及其应用

论述了双链RNA对同源基因的表达进行特异性的封闭而引起的转录后的基因沉默（PTGS/ RNAi）现象, PTGS/ RNAi的作用机理，作用模型，参与这一过程的酶、基因、起始因子和作用因子，介绍了PTGS/ RNAi在基因组功能研究、基因的时空表达调控以及在作物改良等方面的应用。     

错开双圆盘开沟器用于玉米根茬地的试验研究

针对在玉米根茬较大,田地免耕直播时锐角式开沟器易出现挂茬、堵塞等问题,提出了采用错开双圆盘开沟器的方案。该开沟器可一次完成切断秸秆、劈开根茬、开沟施肥、播种的功能,用两圆盘实现传统上用1个圆盘切断秸秆、劈开根茬和2个圆盘开沟的3个圆盘的作用,从而简化播种机结构。通过设计样机,初步试验表明,其切断秸秆能力、入土能力等和机器质量密切相关,当整机配重增加到350kg时,播种机效果良好。     

半固定管道式喷灌系统移管机行走轮平衡状态的研究

对半固定管道式喷灌系统移管机运行过程中的平衡问题进行了分析研究，建立了运动时行走轮稳定状态的数学模型，论证了研制移管机的可行性。     

齿轮三维模型的参数化设计技术

齿轮是汽车、拖拉机中常用的传动零件,在三维设计中,需要对渐开线方程进行转换,由转换后的方程生成齿形,其整个设计过程非常繁琐。本文详述了方程的转换过程及齿轮的参数化设计方法,可以通过输入不同的齿轮参数,即可获得相应的齿轮,从而大大节约设计时间。该方法还可应用于所有系列化的通用件和标准件三维设计中。     

基于直剪试验的稻谷粮堆力学性质研究

为揭示粮仓建设和粮食运输途中粮堆的强度与剪胀特性,开展了在垂直压力为25~200kPa、含水率分别为8.41%、10.59%、13.88%和16.25%条件下的稻谷直剪试验。结果表明:稻谷粮堆剪切变形可分为弹性变形、塑性变形和籽粒压缩3个阶段;不同含水率下,稻谷粮堆强度特性基本符合莫尔-库伦强度准则,随着含水率增加,稻谷粮堆粘聚力逐渐减少,内摩擦角逐渐增大;含水率对稻谷粮堆的剪胀特性影响不明显,但随着垂直压力的增大,其剪缩性越明显;在垂直压力较小的条件下,含水率对稻谷粮堆的抗剪强度影响较大,随着垂直压力的增大,含水率对抗剪强度的影响逐渐减小。研究结果可为粮食仓储和运输、装载粮食机械的设计、粮堆数值仿真建模提供参考。     

太阳能装配式蒙古包供能用多曲面聚光器性能

严寒寒冷地区装配式蒙古包冬季供能多采用生物质燃烧、电加热等方式,无法满足分布式绿色低碳可持续供能的要求。为解决上述问题,该研究提出一种用于装配式蒙古包的新型太阳能多曲面聚光集热供能技术,其具有太阳辐射“接力”供能、热输运阻力小、集热装置与蒙古包围护合而为一、正浮力梯度传热等特点,介绍了技术运行原理及所用多曲面聚光器结构参数,利用光学仿真软件TracePro对聚光器光学性能随时间的变化规律进行了分析,对比研究了实际天气条件下正浮力梯度传热与负浮力梯度传热对聚光器内空气进出口温度、集热量等热性能的影响。结果表明,当径向入射偏角为15°时,聚光器光线接收率约为89.50%,当轴向入射偏角为30°时,聚光器光线接收率为83.47%,相邻不同朝向聚光器在10:30和13:30左右聚光效率复合;在冬季晴天,采用正浮力梯度传热方式的聚光器最大出口温度与最大集热量分别为21.3℃和787.29 W,分别比采用负浮力梯度传热方式的聚光器提升了9.3℃和59.30%。此外,聚光器采用正浮力梯度传热与负浮力梯度传热时的光热转化效率分别为46.81%和35.71%,研究结果可以为装配式蒙古包供能用聚光器集成提...     

变温处理对百合生长发育影响及刺足根螨防治效果

以“索邦”百合(Lilium spp.)的休眠种球为材料,经不同温度和时间的温水浸泡处理,观测百合种球的发芽率、出苗率和营养生长期、盛花期的形态指标以及鳞茎中刺足根螨(Rhizogl yphus echinopus)的发生情况,探究变温处理对百合生长发育的影响和剌足根螨的防治效果.结果 表明:百合种球以48℃温水浸泡3...     

园林有害植物——五爪金龙

五爪金龙(Ipomoea cairica)是旋花科多年生草质藤本植物,原产北美.它生长迅速,攀爬能力强,现已成为我国华南地区仅次于薇甘菊(Mikania micrantha )的草质藤本害草,对广州、深圳及粤东地区的公园绿地、风景林、人工林、果园、茶园、苗圃等造成了极大的危害.文章详细介绍了五爪金龙的形态特征、生物学特性和生态学特征、危害状况以及存在的问题,以期引起人们对该植物足够的警惕,并为有关部门对该植物进行科学有效地防范和治理提供依据.