1例犬埃立克体病的诊治

犬埃立克体是一种重要的人兽共患传染性病原体,可引起犬科动物的犬单核细胞埃立克体病。为确诊1例临床上以发热、精神沉郁、黏膜苍白为主要特征的宠物犬病例,进行了血常规、血液生化检查、实时荧光定量PCR检测、犬埃立克体抗体检查,最终确诊该病例为犬埃立克体感染,使用盐酸多西环素治疗后患犬恢复良好。     

新疆阿克苏地区羊驼毛品质分析

试验旨在研究阿克苏地区引进羊驼的毛绒品质现状。采用相关方法标准对新疆阿克苏地区塔格拉克牧场引进的158只羊驼进行平均纤维直径、毛丛自然长度、净毛率、单纤维强力、原毛油脂含量及卷曲指标的测定，统计分析性别、年龄、被毛颜色对测定指标的影响。结果表明，阿克苏地区引进羊驼毛平均纤维直径23.36 μm，毛丛自然长度106.14 mm，净毛率80.90%，断裂强力8.04 cN，断裂伸长百分比40.11%，原毛油脂含量2.08%，卷曲6.70个/2.5 cm。不同性别的羊驼毛丛自然长度、净毛率、原毛油脂含量差异显著（P<0.05）；不同年龄的羊驼纤维直径、毛丛自然长度、断裂强力及原毛油脂含量差异显著（P<0.05）；不同被毛颜色的羊驼毛丛自然长度、纤维直径、原毛油脂含量及卷曲差异显著（P<0.05）。综上可知，阿克苏地区引进的羊驼在纤维直径、毛丛自然长度方面较优良，且性别、年龄、被毛颜色对羊驼毛纤维遗传性状存在显著影响。     

Administration of magnolol postpones development of hypertension in juvenile spontaneous hypertensive rats

AIM:To investigate the effects of magnolol (MAG) on blood pressure and aortic vasodilatation to insulin in juvenile spontaneous hypertensive rats (SHR). METHODS:Four-week-old male SHR and age-matched normotensive Wistar-Kyoto (WKY) control rats were used. SHR and WKY rats were randomized into 2 groups and treated daily by gavage with vehicle (distilled water) or MAG (100 mg·kg-1·d-1). After 3 weeks of treatment, blood pressure, aortic vasorelaxation, fasting glucose and plasma insulin levels, the expressions of PPARγ and TRB3, and insulin-stimulated Akt/endothelial nitric oxide synthase (eNOS) activation were measured. In vitro, human umbilical vein endothelial cells (HUVECs) were cultured in the medium containing glucose (25 mmol/L) and palmitate (500 μmol/L). RESULTS:Treatment of young SHR with MAG for 3 weeks decreased blood pressure, improved insulin-induced aortic vasodilation, and Akt and eNOS activation , increased PPARγ expression and decreased TRB3 expression. In cultured HUVECs, MAG incubation increased PPARγ exprssion, decreased TRB3 expression, and elevated insulin-induced phosphorylated Akt and eNOS levels and NO production, which were reversed by PPARγ antagonist. CONCLUSION: Treatment of young SHR with MAG at the prehypertensive stage decreases blood pressure via improving vascular insulin resistance that is at least partly attributable to up-regulation of PPARγ, down-regulation of TRB3 and consequently activation of Akt and eNOS in blood vessel .     

床土改良剂对草坪坪床理化特性及坪用性状的影响研究

不同施量的坪床改良剂(YNEC)对单播和混播草坪的床土理化特性和草坪特性改良效果的试验表明:施用坪床改良剂,改善了土壤理化性质,增加了土壤肥力,床土的速效氮(AN)、速效磷(AP)和有机质(OM)的含量增加,呈显著正相关(P<0.01),盐分含量,pH值和土壤容重降低,呈显著负相关(P<0.01);有效改善草坪品质,特别是盖度和绿度提高1～3个分值;对草坪草地上植物量的影响呈非直线型正相关,而对分蘖数影响不显著;最佳施用量为2kg/m2。     

肉用仔兔消化系统发育与机能特点变化的研究

经３４只初生至１２周龄的新西兰肉兔试验，初生兔中部消化器官发育最快，主要是肝的重量占中部器官的６６％或占消化器官的４２％。在前部器官中胃的发育最快，舌相对也比较大，舌的重量占前部器官的３１％或占消化器官的８％，４周龄后舌的重量稳定在占消化器官４％的水平上。初生兔后部消化器官发育较晚，初期作用不大。３周龄前，小肠的重量及长度占胃肠总重及总长的百分比高，小肠生长发育的速度超过胃和大肠。３周龄后，大肠的重量及长度占胃肠总重及总长的百分比增高，尤其在４至８周龄时，由于植物性饲料的刺激作用，使大肠，特别是盲肠的结构和机能发生了明显的变化。仔兔胃内消化有着与仔猪相类似的一段机能发育不全期，２周龄内的仔兔胃内缺乏游离盐酸，对蛋白质不能消化。３周龄的仔兔胃内均已出现游离盐酸，对蛋白质也开始有较弱的消化力，４周龄时，仔兔胃内的酸度和消化力已达断奶后幼兔的水平，６周龄时胃液ｐＨ已达成年兔胃液ｐＨ的稳定水平。     

核酸探针技术在鼠金黄色葡萄球菌流行病学调查中的应用

用金黄色葡萄球菌耐热核酸酶nuc基因特异性核酸探针 ,对江苏和上海 5个实验动物中心送检的 4 2 1份SPF鼠样品进行了检测。有 11份样品为阳性反应结果 ,阳性率 2 6 % ( 11/4 2 1)。结果表明 ,在我国一些实验动物中心SPF鼠群中还存在着金黄色葡萄球菌感染     

基于Zernike矩和神经网络的角点筛选方法

在计算机视觉领域中角点特征的提取具有相当重要的地位，因为角点包含了丰富的信息。本文的目的在于提取某类图像上的重要特征角点。其主要思路是首先利用角点检测方法提取图像中的角点之后，然后再通过Zernike矩和神经网络对它们进行筛选，从而达到自提取特征角点的目的。最后通过实验验证了本算法的有效性。尽管本文是针对某类图像进行了特征点提取的，但相信对于一般情况下的目标特征检测也具有一定的借鉴意义。     

Muscarinic receptor 3 agonist promotes epithelial-meschymal transition in human lung cancer A549 cells by activating PI3K/AKT signaling pathway

AIM: To investigate the possible signaling pathway that promotes epithelial-mesenchymal transition (EMT) of the lung cancer A549 cells stimulated with muscarinic receptor 3 (M3R) agonist carbachol. METHODS: The lung cancer cells A549 were treated with 400 μmol/L carbachol. The morphological changes of the cells were observed under inverted phase contrast microscope. The migration and invasion abilites were measured by Wound healing and Transwell assays. qPCR was used to detect the mRNA level of vimentin and E-cadherin. The protein levels of p-AKT, vimentin and E-cadherin were determined by Western blot. RESULTS: After treatment with carbachol, the A549 cells showed loss of the close connection and the cell morphology was transformed from irregular polygon to spindle-like cells. The results of Wound healing and Transwell assays showed that the migration and invasion abilites of the A549 cells were enhanced. Carbachol increased the vimentin expression and decreased the E-cadherin expression at mRNA and protein level (P<0.05). The phosphorylation of AKT in the A549 cells was up-regulated (P<0.05). These changes was inhibited by M3R antagonist 4-DAMP. CONCLUSION: Carbachol promotes EMT in the human lung cancer A549 cells by activating PI3K/AKT signaling pathway.     

日粮粗纤维水平对35～70 d定安鹅生产性能及器官指数的影响

为研究日粮粗纤维水平对定安鹅生产性能及器官指标的影响,试验选取遗传背景一致、体重相近[(1384.33±0.76)g]的28d定安鹅120只,随机分为3个处理组,每个处理组设4个重复,每个重复10只。分别饲喂粗纤维水平为4.29%、5.29%和6.29%的日粮。试验期为35d。结果表明:(1)5.29%粗纤维组末重和日增重较6.29%粗纤维组分别提高13.57%和27.95%(P<0.05);5.29%粗纤维组采食量比4.29%和6.29%粗纤维组提高5.72%和7.95%(P<0.05);5.29%粗纤维组料重比较6.29%粗纤维组降低16.05%(P<0.05)。(2)5.29%粗纤维组屠宰率、半净膛率、腿肌率和胸肌率分别比4.29%和6.29%粗纤维组分别提高1.64%和2.28%、0.38%和1.03%、0.51%和9.45%、4.31%和6.84%(P>0.05)。腹脂率随着粗纤维水平的提高有下降的趋势(P>0.05)。(3)与4.29%和6.29%粗纤维组相比,5.29%粗纤维组心、肝、胃、肌胃及脾脏指数分别提高5.31%和2.43%、6.15%和5.03%、10.45%和7.29%、11.45%和7.94%、1.65%和2.50%(P>0.05)。综合认为,5.29%粗纤维可使35~70d定安鹅获得较优的生产性能。