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81.
Ko JA Kim YM Ryu YB Jeong HJ Park TS Park SJ Wee YJ Kim JS Kim D Lee WS 《Journal of agricultural and food chemistry》2012,60(24):6210-6216
Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50-60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by (1)H and (13)C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries. 相似文献
82.
The self-assembly of asymmetric ABC triblock copolymers in the ordered structure is investigated using an isothermal-isobaric
molecular dynamics simulation. Unlike symmetric ABC triblock copolymers, more fascinating morphologies are observed in asymmetric
ones because of a larger difference of incompatibility between the components. Various modes of self-assembly in assymmetric
ABC triblock copolymers are also observed depending on the block composition. When the composition of block A is changed from
0.125 to 0.25 at the samef
B
=0.25, the morphological transition from the “cylinder in cylinder” to “cylinders at cylinder” structure is observed in the
simulation. In the case of ABC triblocks withf
B
=0.5, a lamellar-type structure is changed to a cylinder-type structure with increasing the length of block A. When the mid-block
length increases further tof
B
=0.625, the “spheres on cylinder” structure is observed in both the A10B50C20 and A20B50C10 triblocks. From these results, the phase diagram of ABC triblock copolymers can be constructed.
Dedicated to Professor Won-Jei Cho on the occasion of his retirement. 相似文献
83.
Ishida A Ohno K Fukushima K Nakashima K Takahashi M Goto-Koshino Y Fujino Y Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1127-1132
High-mobility group box 1 (HMGB1), a nonhistone chromosomal protein, has recently been suggested as a late mediator of the inflammatory cascade. Blood HMGB1 levels are increased in a number of human diseases, and HMGB1 has been suggested to be a useful marker for disease severity and prognosis. The objective of this study was to assess the clinical usefulness of HMGB1 in dogs. Plasma HMGB1 levels, as well as C-reactive protein (CRP), a typical canine inflammatory marker, were measured in dogs with various diseases, especially systemic inflammatory response syndrome (SIRS), and dogs that had undergone surgery. HMGB1 gradually increased and attained a maximum level 72 hr after surgery, whereas CRP increased rapidly, peaking at 24 hr. Although both HMGB1 and CRP levels were significantly increased in dogs with various diseases compared with the control dogs, no correlation was found between the HMGB1 and CRP values. HMGB1 levels in the SIRS group were significantly elevated compared with those in the non-SIRS group. However, the increase in HMGB1 levels above the reference range was not indicative of SIRS. Instead, the presence of increased HMGB1 and CRP levels above the reference ranges significantly affects the poor outcome of SIRS. The present study indicates that HMGB1 is a novel canine inflammatory marker and is distinct from CRP. However, the additional clinical value of HMGB1 measurement remains unclear, and further studies are warranted. 相似文献
84.
Lee JE Lee BJ Hwang JA Ko KS Chung JO Kim EH Lee SJ Hong YS 《Journal of agricultural and food chemistry》2011,59(19):10579-10585
The dependence of global green tea metabolome on plucking positions was investigated through (1)H nuclear magnetic resonance (NMR) analysis coupled with multivariate statistical data set. Pattern recognition methods, such as principal component analysis (PCA) and orthogonal projection on latent structure-discriminant analysis (OPLS-DA), were employed for a finding metabolic discrimination among fresh green tea leaves plucked at different positions from young to old leaves. In addition to clear metabolic discrimination among green tea leaves, elevations in theanine, caffeine, and gallic acid levels but reductions in catechins, such as epicatechin (EC), epigallocatechin (EGC), epicatechin-3-gallate (ECG), and epigallocatechin-3-gallate (EGCG), glucose, and sucrose levels were observed, as the green tea plant grows up. On the other hand, the younger the green tea leaf is, the more theanine, caffeine, and gallic acid but the lesser catechins accumlated in the green tea leaf, revealing a reverse assocation between theanine and catechins levels due to incorporaton of theanine into catechins with growing up green tea plant. Moreover, as compared to the tea leaf, the observation of marked high levels of theanine and low levels of catechins in green tea stems exhibited a distinct tea plant metabolism between the tea leaf and the stem. This metabolomic approach highlights taking insight to global metabolic dependence of green tea leaf on plucking position, thereby providing distinct information on green tea production with specific tea quality. 相似文献
85.
86.
87.
Kavita Sharma Leonid Asnin Eun Young Ko Eul Tai Lee 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(2):150-160
Onion cultivar “Sunpower” has growing popularity in Korea due to high storability. The present study considers changes in flavonols, sugars, and two amino acids in onion bulbs during a long-term storage in the ambient dark storage room and in glasshouse equipped with a climate control system. Flavonol and sugar contents were found to fluctuate noticeably during the storage period. Amino acid content remained relatively unchanged till the onset of inner sprouting, increasing afterward. Visible sprouts appeared at week 22 in the dark storage room and for four weeks later in the glasshouse. The bulbs lost 20–30% of their weight depending on storage conditions at the end of the storage trials. At the same time, the content of nutraceutics in study expressed on the dry weight basis remained of the same order of magnitude. The nature of observed variations in chemical composition of onions as well as relation of this phenomenon to physiological development of stored onion bulbs is discussed. A conclusion is made that the “Sunpower” onion cannot sustain overwinter storage. 相似文献
88.
89.
广州地区十字花科蔬菜花叶病(主要病毒是芜菁花叶病毒的油菜毒系和芜菁毒系)的越夏寄主,根据11年来的观察特别是1956—1958年间的实地调查结果,主要是小白菜、菜心和西洋菜。在野生植物中曾发现过2株蔊菜和1株荠菜自然感病,说明野生植物不是本病的重要毒源。室内试验结果证明,此病的自然传染媒介为萝卜蚜、桃蚜和普通红蜘蛛。黄条跳(虫甲)、斜纹夜盗蛾和菜粉蝶都不是本病的虫媒,病株的种子不会传病,中国菟丝子也不会传递本病。在带有未腐熟的病菜残体的土里进行直播,长出来的菜苗没有发病的;但是把菜苗移植在这样的土里,会有极小量的植株感病。根据1956年7月至1958年6月,每十日田间调查一次结果:萝卜蚜是广州地区最重要的传病媒介,桃蚜每年只在3—4月间略有发生,作用不大。根据这24个月的田间发病率,萝卜蚜(有翅蚜及无翅蚜)的虫口密度和气候情况,我们认为本地区本病的发生及流行程度主要受降水量和降水天数所影响,并认为可以从降水情况来预测约30日后的病害流行程度。 相似文献
90.
Continuous photografting/crosslinking of polyethyleneglycol dimethacrylate oligomers onto cotton using a water-soluble benzophenone
photoinitiator was investigated. Photografting increased with increasing irradiation dose, oligomer concentration and photoinitiator
concentration. Maximum grafting efficiency of DM 400 and 600 were 83% and 79%, respectively. The photografting increased the
wrinkle resistance of cotton implying surface crosslinking of cotton. Both surface crosslinking and bulk crosslinking of cotton
were accomplished via dual curing of a mixed formulation containing both a thermally curable component (BTCA/SHP) and a UV-curable
component. The wrinkle resistance of the crosslinked cotton was found to be higher when cured by thermal curing after UV curing
rather than by UV curing after thermal curing due to the facile post-polymerization of the UV active component. The presence
of crosslinks in the dually crosslinked cotton was verified with FT-IR and thermogravimetric analysis. 相似文献