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51.
Forest fungi not only have important functions within the forest ecosystem, but picking their fruit bodies is also a popular past time, as well as a source of income in many developing and developed countries. The expansion of commercial harvesting in many parts of the world has led to widespread concern about overharvesting and possible damage to fungal resources. In 1975, we started a field research project to investigate the effects of mushroom picking on fruit body occurrence. The three treatments applied were the harvesting techniques picking and cutting, and the concomitant trampling of the forest floor. The results reveal that, contrary to expectations, long-term and systematic harvesting reduces neither the future yields of fruit bodies nor the species richness of wild forest fungi, irrespective of whether the harvesting technique was picking or cutting. Forest floor trampling does, however, reduce fruit body numbers, but our data show no evidence that trampling damaged the soil mycelia in the studied time period.  相似文献   
52.
Effect of arbuscular mycorrhizal fungi (AMF) on heavy metal tolerance of alfalfa (Medicago sativa L.) and oat (Avena sativa L.) on a sewage-sludge treated soil In pot experiments with a sewage sludge treated soil, the influence of two arbuscular mycorrhizal fungi (AMF) isolates of Glomus sp. (T6 and D13) on plant growth and on the uptake of heavy metals by alfalfa (Medicago sativa L.) and oat (Avena sativa L.) was investigated. Alfalfa showed an increase of biomass with mycorrhizal infection only to a small extent. In oat AMF inoculation increased the growth of both root and shoot by up to 70% and 55% respectively. Mycorrhization raised the P-content and -uptake in alfalfa, but not in oat, in both roots and shoots. Mycorrhizal alfalfa showed lower Zn-, Cd- and Ni-contents and uptake in roots and shoots. The root length was significantly decreased in mycorrhizal alfalfa plants (up to 38%). The translocation of heavy metals into the shoot of mycorrhizal alfalfa was slightly increased. Mycorrhizal infection of oat led to higher concentrations of Zn, Cd and Ni in the root but to less Zn in the shoot. The translocation of heavy metals to the oat shoot was clearely decreased by mycorrhizal colonisation. This may be based on the ability of fungal tissues to complex heavy metals at the cell walls, thus excluding metals from the shoot. This conclusion is supported by the enhanced root length (up to 78%) of mycorrhizal oat plants in this experiment. The mycorrhizal infection seemed to protect plants against heavy metal pollution in soils. It was obvious that different host plants reacted in different ways.  相似文献   
53.
The influence of harvest date and storage method on bruises sensitivity of apples was investigated. The cultivars ‘Braeburn’, ‘Jonagold’ and ‘Golden Delicious’ were harvested at three times in an interval of 10 days. Fruits have been stored at 2°C in normal atmosphere (cold storage) or in film bags (CA-/ULO-storage; CA?=?Controlled Atmosphere, ULO?=?Ultra Low Oxygen) for three months. Monthly fruits were bruised by a penetrometer applying a range of pressures. One day later the appearance of bruises were evaluated. Increasing pressures resulted in bruises of increasing severity. Early harvest led to less severe bruises. No differences were observed in storage conditions, but duration of storage increased bruise sensitivity of ‘Braeburn’. In comparison of the three cultivars ‘Braeburn’ was less sensitive than ‘Jonagold’ and ‘Golden Delicious’.  相似文献   
54.
A dog with polyarthritis, angular joint deformities, and a high serum antinucleolar antibody titer was treated over a period of 20 months. During the clinical course, leukopenia, thrombocytopenia, Coombs' positive hemolytic anemia, and a pemphigus-type skin disorder developed, all of which responded to immunosuppressive therapy. It is not known whether the polysystemic disease in this dog represents a pleomorphic manifestation of canine systemic lupus erythematosus or multiple autoimmune disorders occurring in the same animal.  相似文献   
55.
Fruit characteristics of the disease-resistant bluggoe-type (ABB) cooking bananas (Musa × paradisiaca L.) ‘Saba’ and ‘Pelipita’ were compared with those of the susceptible ‘Currare’ (‘Horn’). Yields of ‘Saba’ and ‘Pelipita’ were similar to those of ‘Currare’; however, ‘Saba’ and ‘Pelipita’ yielded fewer hands with a greater number of small fruit when compared with ‘Currare’. Shoot-tip cultures of both clones were readily initiated on a modified Murashige and Skoog (MS) basal medium supplemented with N6-benzyladenine (BA) in combination with indole-3-acetic acid (IAA). Propagation cultures were initiated by splitting shoot tips along their longitudinal axis and re-culturing the individual pieces to basal medium supplemented with 5 mg l?1 BA. Transfer of axillary shoots to hormone-free medium resulted in rapid and extensive root formation. Plantlet survival after transfer to methyl bromide-treated soil exceeded 90%. Establishment in the field was achieved following procedures normally used for vegetative propagation of this crop. Trial plantings of in vitro propagated ‘Saba’ and ‘Pelipita’ were established in various provinces of Costa Rica for grower evaluation and for future comparison of growth and reproductive development with plants of these and other cultivars propagated from corms.  相似文献   
56.
Summary Plant protection as a problem of plant quality-researchThe use of pesticides —although necessary for plant protection — gives rise to cogent criticism. This refers not only to overapplications of biocidal sprays but also to certain pesticides or herbicides themselves being rather suspect as far as their physiological and biochemical behaviour and/or their peculiar metabolism are concerned. This in turn eventually leads to rather a high accumulation of toxic residues of the pesticides or of their metabolites in food plants, the latter sometimes more toxic than the pesticide itself. Furthermore undesired alterations in plant constituents important to health may occur causing significant depressions in levels of ascorbic acid (spinach) and of carotene (carrots). Chemicals used as plantregulators such as 2,4-D and their derivates may cause a most severe detrimental effect to health (damage to the offspring) even when applied in very small concentrations ranging on nanogram-levels. Therefore the dictum of Paracelsus sola dosis facit venenum does not refer to plantregulators such as 2,4-D and related chemicals. Manifold is the propaganda pro agro- chemicals and their use by manufacturers of pesticides. But they are mostly not trustworthy, erroneous and easily to refute by significant facts.  相似文献   
57.
The ubiquitous muscle protein tropomyosin has been identified as the major shrimp allergen and is suggested to be a cross-reacting allergen. Previously, only a few methods for the detection of tropomyosin in food have been published. A quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of tropomyosin from crustaceans in foods has been developed and validated. A polyclonal rabbit antitropomyosin capture antibody and the biotinylated conjugate of the same antibody for detection were the basis for the ELISA, which was specific for crustaceans. The ELISA was able to quantitate tropomyosin in various food matrixes, had a detection limit of 1 microg/g, and cross-reacted to some extent with cockroach. Recoveries ranged from 63 to 120%, and the intra and interassay coefficients of variation were <6 and <14%, respectively.  相似文献   
58.
Data on reproductive traits in a cultured population of brown trout (Salmo trutta L.) in southern Chile were collected over three consecutive reproductive seasons: 1999, 2000 and 2001, corresponding to the first (at 3 years), second and third spawnings in females. Data were collected from individual females (n=238, 273 and 169 respectively). The reproductive season lasted mainly from June to August. The peak (July) tended to increase with each season (55.46%, 62.27% and 80.81% of total spawning fish respectively). Female body weight (470.8±102.5, 735.0±150 and 1263.9±263.4 g), total fecundity (1182±344, 1904±595 and 2744±605) and egg diameter (4.64±0.11, 4.67±0.27 and 5.24±0.12 mm) increased significantly (P<0.01) over successive reproductive seasons, particularly between the second and third spawnings. Relative fecundity, on the other hand, decreased significantly with each season (3577±471, 2591±900 and 2181±360). Following analysis of the variables over the three seasons, the correlation pattern of female body weight with total fecundity (r=0.91, n=458; P<0.001) and relative fecundity (r=?034, n=451; P<0.001) proved similar to that described in other salmonid females. Fertilization rate and survival to the eyed egg stage were also positively correlated (r=0.73, n=453; P<0.001). The systematically high values obtained for these latter variables over the seasons evaluated (consistently above 90%) are clearly greater than those we registered in other species of salmonids bred in Chile under similar conditions and suggest highly efficient biological variables that determine the gamete fertilization of this species. The brown trout is, therefore, an interesting potential aquaculture resource in Chile.  相似文献   
59.
60.
Videography and night vision equipment were used to observe the diurnal and nocturnal activities of American white pelicans Pelecanus erythrorhynchos ( N =6) foraging on three experimental (0.04 ha) channel catfish ( Ictalurus punctatus ) aquaculture ponds in March 2001. Captive pelicans spent most time foraging per hour from 1700 through 2300 h (CST). No foraging was observed from 0700 through 0800 h. Fish captures per hour were greatest from 1700 through 1900 h. On average, captive pelicans ( N = 5) consumed 1.0 ± 0.2 kg of catfish per bird per day during the 10-d foraging trial. This consumption corresponds with an average intake of over 60,000 kJ of energy per bird during the trial. Relative to morning hours, the average number of bill dips per min was greater during afternoon foraging bouts. Fish captures per bill dip, however, did not differ among trial hours. Thus, pelicans were observed to increase foraging effort subsequent to 1500 h and thereby consumed 224 to 532 catfish (average = 313 ± 74 fish; N = 4) during the 10-d foraging trial in ponds stocked with approximately 74,000 catfish/ha.  相似文献   
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