Amplified fragment length polymorphism- and simple sequence repeat-based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat

The greenbug, Schizaphis graminum (Rondani), is the most economically damaging aphid pest of wheat in the southern Great Plains of the USA. In this study, the single, dominant greenbug resistance gene, Gb3, was molecularly tagged and genetically mapped using amplified fragment length polymorphism (AFLP) and simple sequence repeat(SSR) markers. Three AFLP loci were associated with the Gb3 locus in linkage analysis with 75 F_2:3 families from the cross between two near‐isogenic lines (NILs) for Gb3,‘TXGBE273’ and ‘TXGBE281′. Two of these loci, XMgcc Pagg and Xmagg Patg cosegregate with Gb3 in the population analysed. Further analysis indicated that XMgcc Pagg and Xmagg Patg are specific for the Gb3 locus in diverse genetic backgrounds. Two SSR markers, Xgwm111 and Xgwm428 previously mapped in wheat chromosome 7D, were shown to be linked with Gb3, 22.5 cM and 33.1 cM from Gb3, respectively, in an F₂ population of ‘Largo’בTAM 107’, suggesting that Gb3 is located in the long arm of chromosome 7D. The two AFLP markers cosegregating with Gb3 are valuable tools in developing molecular markers for marker‐assisted selection of greenbug resistance in wheat breeding.     

高温木质素降解菌Geobacillus caldoxylosilyticus J16的筛选及其产酶发酵性质研究

从张家界、白云山采集到的朽木及落叶中,经分离、纯化后,获得了一株高温木质素降解菌,命名为地芽孢杆菌（Geobacillus caldoxylosilyticus J16）,此菌嗜热,耐高温,只降解木质素且不降解纤维素,对造纸业和可再生能源产业具有重要意义。本文通过对木质素中两种酶木质素过氧化物酶、锰过氧化物酶活性的研究,确定了木质素过氧化物酶的最适温度为65℃,最适PH为4,在55℃至70℃之间时酶活力较其他温度高且较稳定;锰过氧化物酶最是温度为60℃,最适pH为3在温度为55℃至65℃之间时,活力较其他温度高。作者发酵了黄豆杆、玉米杆、芝麻杆、油菜杆、锯末、小麦杆这六种农业废弃物,通过发酵前后的比较,说明了J16菌株对木质素降解的最大减少量为7.5%。上述数据显示此菌可以应用于废弃秸秆的处理和造纸厂污水的处理,对环保有重要意义.     

甘蔗间种菜用大豆对甘蔗产量、品质及间种后综合经济效益的影响

以广西区种植面积最大的‘新台糖22’、‘桂春2号’、‘柳豆3号’和‘桂春8号’为间作套种试验材料,研究探索不同大豆品种、不同播期及不同间种密度对甘蔗产量、品质及综合经济效益的影响。试验结果表明,在同等管理和施肥条件、种地养地、保证甘蔗品质和产量的前提下,以第2播期间种2行中熟大豆品种效果最好。综合经济效益最高的是第2播期间种2行的晚熟大豆品种。     

A Comparative Study of Confined Concrete Models under Cyclic Loading

In this paper,four models are selected as the representatives and applied to nonlinear dynamic analysis program for space frames which is based on the beam-column elements using the finite element flexibility method and the fiber model.Park's test on combined axial and bending columns under cyclic loading are taken as the calibrations,comparisons to material and structural member level are drawn and the analytical results of various models are discussed.     

rep-PCR法对稻瘟病菌有性后代随机群体的评价

构建稻瘟病菌有性后代随机群体是研究目的基因遗传特点，乃至克隆目的基因的重要基础，但是在建立有性后代随机群体时，可能会有无性后代污染。采用先分离单个子囊，待其长成菌落并产生分生孢子后，再分离单个萌发的分生孢子的方法构建稻瘟病菌有性后代随机群体。利用基于重复DNA序列Pot2的rep-PCR方法对分离得到的有性后代进行DNA指纹分析，并评价构建的稻瘟病菌有性后代群体的质量。结果表明，构建的有性后代随机群体共包含176个后代。每个后代个体的DNA指纹图谱均与两亲本的DNA指纹图谱不一致；有性后代之间的DNA指纹图谱也彼此不一样，说明得到的有性后代个体都是由子囊孢子萌发形成的，且分离自不同的子囊。GUY11的rep-PCR扩增片段中的5个特异性片段的分离比例也都符合1:1的期望值，是按单个位点标记分离的，与已知的结果相吻合，说明有性后代随机群体的质量较高，不存在偏离现象，是一个较为理想的群体。同时，研究结果还得出基于Pot2的rep-PCR方法可以用来快速评价稻瘟病菌有性后代群体的质量。     

槲皮素对苎麻、涤纶的吸附性能初步研究

本文以生物黄酮配基槲皮素为分散染料,对苎麻分别在77℃乙醇染浴中和在93℃水染浴中进行了染色实验,并与合成纤维涤纶进行对比,为苎麻和涤纶用天然染料进行染色的工艺选择提出了初步方案。结果表明槲皮素是一种理想的环保天然分散染料。     

Effect of Different Temperature and Anaerobic/Aerobic Conditions on the Decay Characteristics of PAOs

In order to analyze the effect of different temperatures on PAOs decay, a lab scale EBPR culture with PAOs over 90%±2% of the entire bacterial was used under different conditions (10℃ anaerobic, 10℃ aerobic, 20℃ anaerobic, 20℃ aerobic). The results show that the decay rate increases with the increase of temperature; the average rate during 1~9 days is (respectively: 10℃ anaerobic, 10℃ aerobic, 20℃ anaerobic, 20℃ aerobic) 0.053/d, 0.50/d, 0.072/d, and 0.145/d; the cell death rate is 0.019/d, 0.017/d, 0.019/d, and 0.03/d, which accounts for 35.8%, 34%, 26.4%, and 20.7% of the decay rate, respectively. The concentration of PHA and glycogen decreases during the decay period. And Glycogen degrades quickly in anaerobic conditions than in aerobic conditions under the same temperature. The glycogen degrade rate is higher with the increase of temperature under the same anaerobic and aerobic conditions.     

浅圆仓布料器减缓大豆发热结块情况的效果分析

研究分析浅圆仓布料器延缓高温高湿地区大豆储存期间发热结块情况。结果表明:浅圆仓布料器能明显提高大豆全面通风的均衡性,并有效延缓局部异常发热结块。     

不同烟草品种对卡那霉素抗性及耐盐性的差异

研究了卡那霉素对烟草4个品种叶片芽分化和根再生的影响,并对筛选出的卡那霉素抗性最强和最弱的品种进行抗盐能力分析,结果发现,品种CV87对卡那霉素非常敏感,4mg/L或6mg/L浓度下,叶片芽分化率或茎段根再生率均为零,而品种T12却对卡那霉素具有很高耐受性,在300mg/L浓度时,叶片才失去分化能力,而茎段根再生受阻的卡那霉素浓度则高达400mg/L。4个品种抗卡那霉素能力为T12〉SD-1〉NC89〉CV87。高浓度（≥1.0%）盐胁迫,品种CV87受伤害严重,表现为叶片黄化、皱缩并增厚,根再生能力丧失,且叶绿素、脯氨酸、丙二醛及细胞膜透性相对值指标都显著或极显著地低于或高于品种T12,表明品种T12的抗盐能力高于品种CV87。     

复杂泵系统边界条件建模及水锤计算

本文对复杂泵系统水锤数学模型进行了分析与推导 ,建立了带盲管的多台并联泵向不同阻力特性的多管道供水的水锤计算方程组并对该非线性双曲方程进行了求解 ,对该系统三次水锤事故进行了模拟计算 ,找出了三次事故的原因。