Mutations in U4atac snRNA, a component of the minor spliceosome, in the developmental disorder MOPD I

Small nuclear RNAs (snRNAs) are essential factors in messenger RNA splicing. By means of homozygosity mapping and deep sequencing, we show that a gene encoding U4atac snRNA, a component of the minor U12-dependent spliceosome, is mutated in individuals with microcephalic osteodysplastic primordial dwarfism type I (MOPD I), a severe developmental disorder characterized by extreme intrauterine growth retardation and multiple organ abnormalities. Functional assays showed that mutations (30G>A, 51G>A, 55G>A, and 111G>A) associated with MOPD I cause defective U12-dependent splicing. Endogenous U12-dependent but not U2-dependent introns were found to be poorly spliced in MOPD I patient fibroblast cells. The introduction of wild-type U4atac snRNA into MOPD I cells enhanced U12-dependent splicing. These results illustrate the critical role of minor intron splicing in human development.     

盐胁迫对7种草本地被植物生长及光合特性的影响

采用盆栽试验法,研究了不同浓度N aC l盐胁迫对二色补血草(L im on ium bicolor)、佛甲草(S edumlineare T hunb.)、千叶蓍(A ch illea m illef olium)、丛生福禄考(P h lox subu la ta)、百里香(T hym us m ong olicus R onn)、地被石竹(D ianthus acicu laris)和高羊茅(F estuca arund inacea Schreb)等7种地被植物生长及光合特性的影响。结果表明,盐胁迫使参试植物生长受到抑制,但受抑制程度有明显区别,相对生长量由大至小依次为高羊毛(86.52%)>二色补血草(78.78%)>千叶蓍(65.38%)>地被石竹(56.68%)>百里香(41.05%)>佛甲草(32.25%)>丛生福禄考(0.00%);盐胁迫下7种植物的光合速率(P n)和气孔导度(G s)均下降,但下降幅度在各种植物间差异较大,高羊茅和二色补血草的下降幅度较小,佛甲草和丛生福禄考的较大;7种植物胞间CO2浓度(C i)的变化规律较复杂。综合分析7种草本地被植物的相对生长量和光合指标可知,其耐盐性由强至弱依次为:二色补血草>高羊茅>千叶蓍>地被石竹>百里香>佛甲草>丛生福禄考。     

关于高等职业教育专业设置规范化的思考

培养适应 2 1世纪我国社会主义现代化建设要求的高素质专门人才 ,是高等职业教育贯彻落实国家教育方针的重要任务 ,作为高等职业教育与社会结合点的专业 ,是保证培养适销对路人才的重要环节 ,因此 ,加强高等职业教育专业的规范化管理有着重要的实践意义     

宝珠梨疫腐病病原菌研究

 宝珠梨疫腐病是云南省的一种新记录病害,其病原菌为恶疫霉Phytophthora cactorum (Lebert et Cohn) Schrot.适宜恶疫霉分离培养的培养基是番茄汁培养基、V8汁培养基和PDA培养基。适宜恶疫霉生长的pH值范围在3～10之间,最适pH值为5～6,适宜生长的温度范围为在10～30℃之间,最适生长的温度为20～25℃. 恶疫霉对果糖、麦芽糖、蔗糖等碳源的利用好于其它碳源,对酵母膏、天门冬酰胺、牛肉膏等氮源的利用好于其它氮源。     

河南省豆科植物根瘤菌表型多样性研究

在河南省根瘤菌资源调查的基础上 ,选取 2 9株快生根瘤菌和 34株参比菌株 ,进行了 10 2项表型性状测定和聚类分析。结果表明 :不同地理来源、甚至同一地理来源或同种寄主的不同菌株在碳氮源利用、抗生素抗性、耐逆性等方面存在着较大的多样性。有 7株菌对链霉素、青霉素和白霉素有较强的耐受性 ;有 2 3株和 2 5株菌可分别耐受 2 .0 g/ L的香酚兰和中性红 ;有 2株菌可在含 5 0 g/ L Na Cl的 YMA培养基上生长 ;有 6株菌在 p H值 11条件下能够生长。在 84 %的相似性水平上未知菌株构成了 2个独立的表观群 ,其中 Cluster 1有 7株菌 ,中心菌株为 H 0 38;Cluster 2有 3株菌 ,中心菌株为 H0 2 6。     

玉米氮、磷、钾肥料效应的研究

在吉林省蛟河市对玉米进行"3414"肥效试脸,拟合了肥料效应三元二次方程和一元二次方程。通过三元二次方程得出,在该土壤条件下氮、磷、钾最高施肥量为(kg/hm2)N-P2O5-K2O=212.24-57.0-78.3,对应的最高产量为10 551.5 kg/hm2;最佳施肥量(kg/hm2)N-P2O5-K2O=168.4-55.4-63.2,对应的产量为10 432.7kg/hm2。通过拟合一元二次方程得出,在该土壤条件下氮、磷、钾最高施肥量为212.1 kg/hm2、67.0 kg/hm2、64.0kg/hm2;最佳经济施肥量184.1 kg/hm2、63.0 kg/hm2、60.3 kg/hm2。     

葡萄白藜芦醇主要衍生物的研究进展

 葡萄白藜芦醇的衍生物都源于白藜芦醇,但结构又不同于白藜芦醇,其中有些化合物具有与白藜芦醇相同或相似的药理活性,有些甚至强于白藜芦醇,在医学以及植物抗病等研究领域备受关注。因此,综述了这些物质的抗病作用、保健功能、检测方法以及诱导合成等方面的研究进展,以期为深入研究奠定基础。     

Effect of prostate-specific membrane antigen on phospho-ERK,growth and migration of prostate cancer LNCaP cells

AIM: To study the expression of prostate-specific membrane antigen (PSMA) on the level of phospho-ERK, cell growth and migration of prostate cancer LNCaP cells.METHODS: The method of silencing PSMA was established by lentivirus-mediated RNAi in our early experiment. The cells were divided into 3 groups.In experimental group, the expression of PSMA in LNCaP cells was stably blocked by lentivirus-mediated RNAi. In negative control group, the cells were transfected with lentivirus-mediated control RNAi (without any interference to PSMA).The normal LNCaP cells served as blank control. The cells in these 3 groups were cultured in both 2 environments: normal medium and medium with PD98059 (an inhibitor of ERK phosphorylation). The phospho-ERK was detected by Western blotting and immunocytochemistry. Furthermore, the growth and migration of the cells were evaluated by MTT and transwell assays,respectively.RESULTS: In normal medium, the expression of phospho-ERK was attenuated in experimental group (P<0.05) and the quantity of "positive" cells was less than those in other 2 groups (P<0.05). Furthermore, the growth curves of the cells showed that the growth ability in experimental group was significantly decreased (P<0.05, after 48 h) and the migration ability in experimental group was reduced (P<0.05). In the inhibitory medium, the cells in all 3 groups expressed phospho-ERK at a lower level. Moreover, the abilities of growth and migration in these 3 groups were poorly displayed. These inhibitory effects on phosphorylation of ERK were similar to the cells in experimental group cultured in normal medium.CONCLUSION: PSMA may play a role in up-regulation of phospho-ERK and it may take an advantage in growth and migration of prostate cancer LNCaP cells.     

螺虫乙酯对烟粉虱的防治效果评价

采用室内毒力测定与田间试验相结合的方法,研究了螺虫乙酯（Movento）对烟粉虱的防治效果。室内毒力测定结果表明：96 %螺虫乙酯原药对烟粉虱卵和若虫都具有非常高的杀虫活性,LC50分别为18.91 mg?L-1和14.97 mg?L-1,与对照95 %吡虫啉没有显著差异|但对烟粉虱成虫的杀虫效果并不十分理想,LC50达到了453.34 mg?L-1,且显著高于对照95 %吡虫啉。田间试验结果表明：24 %螺虫乙酯悬浮剂（SC）对烟粉虱有良好的防效和持效性,且螺虫乙酯与Biopower（助剂）混用的防效明显优于螺虫乙酯单独使用的效果|螺虫乙酯与0.2 %〔0.24 g?（667 m2）-1〕Biopower混用,药后1～28 d对烟粉虱的防效分别为64.91 %～66.92 %、82.00 %～82.59 %、88.11 %～89.97 %、89.99 %～93.94 %和91.10 %～93.56 %。     

黄土地区深路堑边坡稳定性分析及抗滑桩设计

原状黄土的抗剪强度与黄土的干容重、含水量、粘粒含量等物理力学性质有关.通过对具体参数指标的分析,确定了不同区域内黄土的土性参数,进而提出了深路堑边坡的稳定性分析及打滑桩设计.