荔枝FKBP16-2基因启动子的克隆与瞬时表达分析

前期研究中发现了一个果肉低表达而叶片中高表达的荔枝基因FKBP16-2。本文克隆了该基因1 578 bp的启动子片段并对其功能进行了初步分析,结果表明:荔枝FKBP16-2基因启动子序列中含有大量的TATA-box和CAAT-box保守元件,以及TCA-element,ARE,HSE,GCN4_motif,O2-site等各种转录调控相关的顺式作用元件。该启动子能驱动GUS基因在荔枝的花、叶、根、果皮以及种子中表达而在果肉中不表达,表达具有组织特异性。     

水分胁迫下转DREB3基因抗旱大豆的生理反应

采用田间试验方法,在苗期和初花期自然状态、水分胁迫以及充足灌水条件下,研究了转DREB3基因抗旱大豆叶片、茎、根中超氧化物歧化酶(SOD)活性,丙二醛(MDA)、脯氨酸和蔗糖含量的变化。结果表明,在自然状态下,苗期水分胁迫第5天时,转DREB3基因抗旱大豆叶片SOD活性显著高于受体大豆东农50,初花期无明显变化;苗期和初花期转DREB3基因抗旱大豆叶片、茎和根中丙二醛、脯氨酸含量与受体大豆东农50相比无明显差异;苗期转DREB3基因抗旱大豆叶片和根中蔗糖含量显著低于受体大豆东农50,初花期差异不显著。在水分胁迫条件下,转DREB3基因抗旱大豆SOD活性显著高于受体大豆东农50,丙二醛含量显著低于受体大豆东农50,初花期水分胁迫第10天时叶片和茎中脯氨酸含量显著高于受体大豆东农50,蔗糖含量显著低于受体大豆东农50。充足灌水管理下,转DREB3基因抗旱大豆与受体大豆东农50相比,苗期和初花期叶片、茎和根中SOD活性、丙二醛和脯氨酸含量无明显差异;苗期转DREB3基因抗旱大豆叶片中蔗糖含量显著低于受体大豆东农50,但在初花期水分胁迫10天时转DREB3基因抗旱大豆叶片中蔗糖含量显著高于受体大豆东农50,苗期和初花期茎和根中蔗糖含量无显著差异。     

不同比例生物有机肥和复合肥混施对重庆地区烟叶农艺性状和品质的影响

为研究不同比例生物有机肥和复合肥混施对烟草农艺性状及品质的影响，采用田间小区试验，分别设置3种不同施肥处理，分析烟草不同生育期农艺性状、烟叶物理性状、烟叶外观等以及烟叶烟碱、总糖、还原糖等烟草品质指标。结果表明：单施复合肥处理(CK)前期烟草农艺性状优于不同比例生物有机肥和复合肥混施处理(T1和T2)，平顶期T1和T2的农艺性状则较CK组有明显优势；烟草中部叶片物理性状表现为T1处理最优，T2次之，CK最差；烟叶品质方面，T2处理烟叶外观质量有所改善，但其化学成分烟碱含量降低。烟叶品质总体表现为T2最优，     

仙草多糖提取工艺条件优化研究

采用水提法浸提仙草，以仙草多糖的提取率为指标，研究料液比、浸提温度、浸提时间及浸提次数4个因素对其提取率的影响，并从中挑取合适的因素水平进行正交试验及SPSS分析。结果表明：在这4个因素中，对提取率影响最大的因素是浸提温度，然后是浸提时间，再次是料液比，最后是浸提次数；在料液比1:60、温度100 ℃、浸提时间3 h的最优条件下，仙草多糖的提取率高达17.89 %，此时仙草多糖的含量为44.13 %。     

菠菜新品种蔬菠2号的选育

蔬菠2号是以雌性系221为母本、自交系213为父本配置的菠菜一代杂交种。该品种植株直立,生长势强,叶片大、深绿色、尖圆形,叶面平展;植株生长速度较快,适于早春露地、秋露地及设施栽培。     

Functional porous carbons from waste cotton fabrics for dyeing wastewater purification

A new functional porous carbons (PC-WF) is prepared by activation-pyrolysis method use waste cotton fabrics (WF) as an abundant, cheap and available precursor for removal of Brilliant Crocein (BC-GR) and Cationic Red 2GL (CR-GL) from aqueous phase. The PC-WF was characterized by BET, FTIR, SEM, and XRD techniques, the surface area, total pore volume, average pore diameter was found as 1463.5 m² g^-1, 0.783 cm³ g^-1 and 2.14 nm, respectively. The influences on BC-GR and CR-GL adsorption of various experimental factors such as initial concentration and temperature were investigated. Adsorption kinetics was found to be best represented by the pseudo-second order model. The adsorption capacity was 319.8 mg g^-1 for BC-GR and 842.5 mg g^-1 for CR-GL at 30 °C, respectively. The results indicate that for waste cotton fabrics in particular, the practical application of this process to the production of porous carbon would be possible.     

美国夏威夷组织培养技术考察报告

对美国夏威夷组织培养技术的应用及现状进行考察学习，及时总结，以期对我国组织培养技术的应用提供借鉴。     

青花菜肉桂酰辅酶A还原酶基因的克隆与表达特征分析

为研究肉桂酰辅酶A还原酶在青花菜生长发育中的作用,采用RT-PCR技术克隆青花菜Bo CCR基因全长c DNA序列,并利用q RT-PCR检测其在不同器官中的表达模式。结果表明:Bo CCR基因开放阅读框为987 bp,推导编码328个氨基酸。预测蛋白分子量为36.86 ku,PI值为6.04。该蛋白亲水性氨基酸多于疏水性氨基酸,为亲水性蛋白。高级结构预测表明青花菜Bo CCR蛋白具有完整的二级结构,三级结构的α-螺旋主要位于外部,β-折叠位于内部。分子进化分析显示CCR蛋白在植物进化过程中,各属形成单独的分枝,可以区分其亲缘关系。荧光定量技术检测到青花菜的Bo CCR基因在花蕾发育早期表达最高,推测该基因可能与青花菜花粉发育相关。     

甘蔗黄叶病毒与花叶病毒CP基因RNAi载体构建与转化甘蔗研究

甘蔗黄叶病和甘蔗花叶病是我国甘蔗最主要的病毒病,感病品种产量下降和品质变劣,传统方法难以防治。RNA干扰技术使培育抗病毒甘蔗品种成为可能。本研究根据甘蔗黄叶病毒和侵染甘蔗的高粱花叶病毒结构与功能特性,广泛收集不同来源病毒分离物CP基因序列,经过比对选取保守序列作为干扰序列。通过在序列两端添加酶切位点,合成并连接成双价甘蔗黄叶病和花叶病毒干扰序列,然后构建到中间载体p HANNIBAL上,形成双价RNAi干扰结构,最后插入到p ART27上形成干扰表达载体。利用基因枪轰击甘蔗品种福农15号愈伤组织进行转化,经G418筛选获得抗性再生植株。通过提取DNA和RNA分析,证实双价RNAi干扰结构不仅以不同拷贝整合到甘蔗基因组中,而且得到了转录表达。     

Optimum Level of Dietary n‐3 Highly Unsaturated Fatty Acids for Juvenile Sea Cucumber,Apostichopus japonicus

A feeding trial was conducted to estimate the optimum level of dietary n‐3 highly unsaturated fatty acids (HUFAs) for juvenile sea cucumber, Apostichopus japonicas, based on growth performance and fatty acid compositions. Diets with five n‐3 HUFAs levels (0.15, 0.22, 0.33, 0.38, and 0.46%) were fed to sea cucumber juveniles (1.97 ± 0.01 g) once a day for 60 d. The sea cucumbers fed diets containing 0.22% n‐3 HUFAs showed significantly (P < 0.05) higher body weight gain, feed efficiency, and protein efficiency ratio than the sea cucumbers fed diets containing 0.15% n‐3 HUFAs, but not significantly different (P > 0.05) from those of sea cucumbers fed diets containing 0.33, 0.38, and 0.46% n‐3 HUFAs. The sea cucumbers fed diets containing 0.46% n‐3 HUFAs showed significantly (P < 0.05) higher eicosapentaenoic acid and saturated fatty acid than the sea cucumber fed diets containing 0.15% n‐3 HUFAs, but not significantly different (P > 0.05) from those of sea cucumbers fed diets containing 0.22, 0.33, and 0.38% n‐3 HUFAs. The results of growth performance and n‐3 HUFA compositions of body wall indicated that the optimum level of dietary n‐3 HUFAs for juvenile sea cucumber is between 0.22 and 0.46%.