全文获取类型
收费全文 | 116336篇 |
免费 | 20402篇 |
国内免费 | 30876篇 |
专业分类
林业 | 12660篇 |
农学 | 12610篇 |
基础科学 | 11483篇 |
38326篇 | |
综合类 | 47689篇 |
农作物 | 8828篇 |
水产渔业 | 7897篇 |
畜牧兽医 | 14641篇 |
园艺 | 4607篇 |
植物保护 | 8873篇 |
出版年
2024年 | 1214篇 |
2023年 | 1760篇 |
2022年 | 3048篇 |
2021年 | 3264篇 |
2020年 | 4657篇 |
2019年 | 7768篇 |
2018年 | 6965篇 |
2017年 | 8561篇 |
2016年 | 8801篇 |
2015年 | 9469篇 |
2014年 | 8643篇 |
2013年 | 9434篇 |
2012年 | 10146篇 |
2011年 | 9524篇 |
2010年 | 8246篇 |
2009年 | 7391篇 |
2008年 | 6322篇 |
2007年 | 6241篇 |
2006年 | 5481篇 |
2005年 | 4767篇 |
2004年 | 3614篇 |
2003年 | 3168篇 |
2002年 | 2872篇 |
2001年 | 2534篇 |
2000年 | 2278篇 |
1999年 | 2255篇 |
1998年 | 1980篇 |
1997年 | 1852篇 |
1996年 | 1669篇 |
1995年 | 1665篇 |
1994年 | 1579篇 |
1993年 | 1299篇 |
1992年 | 1271篇 |
1991年 | 1109篇 |
1990年 | 861篇 |
1989年 | 809篇 |
1988年 | 686篇 |
1987年 | 478篇 |
1986年 | 438篇 |
1985年 | 239篇 |
1984年 | 218篇 |
1983年 | 202篇 |
1982年 | 202篇 |
1981年 | 252篇 |
1980年 | 196篇 |
1979年 | 151篇 |
1978年 | 130篇 |
1975年 | 109篇 |
1965年 | 137篇 |
1964年 | 141篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
171.
RAPD技术对地方鸡种群体遗传结构的分析 总被引:16,自引:1,他引:15
利用RAPD技术对4个地方鸡种和1个引进鸡种的群体遗传结构进行分析,通过筛选的5个随机引物OPH-02、OPH-05、OPH-13、OPH-16、OPG-07对5个鸡种的池DNA进行多态性研究,结果表明:5个引物共产生条带61个,扩增产物片段的长度一般从150bp-4kb。 相似文献
172.
Nephrotoxicity of amphotericin B in dogs: a comparison of two methods of administration. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S I Rubin D R Krawiec H Gelberg R D Shanks 《Canadian journal of veterinary research》1989,53(1):23-28
Two methods of administration of amphotericin B were compared for their ability to produce nephrotoxicity in 12 dogs. Six dogs received six alternate day doses of amphotericin B: 1 mg/kg administered as a rapid bolus in 25 mL 5% dextrose in water. Another six dogs received alternate day treatments of the same dose of amphotericin B in 1 L 5% dextrose in water over 5 h. Both treatment groups experienced significant reductions in glomerular filtration rate, as measured by inulin clearance, 24 h endogenous creatinine clearance, serum creatinine and serum urea. This reduction in glomerular filtration rate was most marked in the group receiving the drug as a rapid bolus. The inulin clearances decreased from 3.54 +/- 0.30 mL/min/kg (means +/- SEM) on day 0 to 1.15 +/- 0.25 mL/min/kg on day 12 in the slow infusion group and from 3.24 +/- 0.25 mL/min/kg on day 0 to 0.46 +/- 0.11 mL/min/kg on day 12 in the rapid bolus group. Renal lesions characteristic of amphotericin B administration were observed in all dogs tested. The dogs which received amphotericin B as a rapid bolus had a significantly greater number of tubular lesions than the slow infusion group. Systemic side effects, such as vomiting, diarrhea and weight loss, were observed in both treatment groups but were most severe in the rapid bolus group.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
173.
Y Zhou V Moennig C O Coulibaly J Dahle B Liess 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(1):76-80
Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues. 相似文献
174.
采取盆栽遮雨人工定量灌水的方法,探讨了常规晚稻和杂交晚稻前后两大生育时期不同灌水量对生育和产量的影响.结果表明:两类型晚稻品种前后两个生育时期节水灌溉都能达到高产之目的,但不同类型和不同生育时期的最佳灌水量和节水效果不同. 相似文献
175.
W M Kuzon Jr J D Rosenblatt B R Pynn P J Marchetti M J Plyley N H McKee 《Canadian journal of veterinary research》1989,53(2):125-132
The purpose of this study was to determine whether there were differences in skeletal muscle properties in the hindlimb muscles of different types of dogs. Muscle samples were obtained from the gracilis, sartorius cranial head, sartorius caudal head and tibialis anterior muscles of mixed-breed and hound-type dogs and Beagles. Fiber type, fiber size and capillary morphometry determinations of each muscle from each dog were made from sections stained for myofibrillar ATPase activity. Individual animals were bilaterally symmetric for all measured variables. Fiber type, fiber size and capillary geometry varied between dogs of a given type and muscles within a given dog. There were no differences between dog types for fiber type or fiber size; significant variation in log(muscle)/log(body) mass ratios between dog types was observed for all muscles. The results indicate that for a given muscle, significant variation can occur in skeletal muscle characteristics between different types of dogs and that these differences can be independent of differences in exercise history. 相似文献
176.
刺槐抗旱造林技术的研究 总被引:2,自引:0,他引:2
在不同类型区及不同立地条件下,研究了提高剌槐成活率和生长量的抗旱造林技术。结果表明,采用径流集存、吸水剂蘸根、覆膜覆草及幼林地少量施肥技术,均可显著提高剌槐造林成率与生长量,提高幅度为2.0%-8.9%和14.5%-87.5%。采用混草技术可增收可观的优质牧草,经济效益和生态效益显著。 相似文献
177.
178.
Biofilm bacteria: formation and comparative susceptibility to antibiotics 总被引:17,自引:0,他引:17
下载免费PDF全文
![点击此处可从《Canadian journal of veterinary research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Merle E. Olson Howard Ceri Douglas W. Morck Andre G. Buret Ronald R. Read 《Canadian journal of veterinary research》2002,66(2):86-92
The Calgary Biofilm Device (CBD) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. The minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin G, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative bacteria were determined. Bacterial biofilms were readily formed on the CBD under selected conditions. The biofilms consisted of microcolonies encased in extracellular polysaccharide material. Biofilms composed of Arcanobacterium (Actinomyces) pyogenes, Staphylococcus aureus, Staphylococcus hyicus, Streptococcus agalactiae, Corynebacterium renale, or Corynebacterium pseudotuberculosis were not killed by the antibiotics tested but as planktonic bacteria they were sensitive at low concentrations. Biofilm and planktonic Streptococcus dysgalactiae and Streptococcus suis were sensitive to penicillin, ceftiofur, cloxacillin, ampicillin, and oxytetracycline. Planktonic Escherichia coli were sensitive to enrofloxacin, gentamicin, oxytetracycline and trimethoprim/ sulfadoxine. Enrofloxacin and gentamicin were the most effective antibiotics against E. coli growing as a biofilm. Salmonella spp. and Pseudomonas aeruginosa isolates growing as planktonic populations were sensitive to enrofloxacin, gentamicin, ampicillin, oxytetracycline, and trimethoprim/sulfadoxine, but as a biofilm, these bacteria were only sensitive to enrofloxacin. Planktonic and biofilm Pasteurella multocida and Mannheimia haemolytica had similar antibiotic sensitivity profiles and were sensitive to most of the antibiotics tested. The CBD provides a valuable new technology that can be used to select antibiotics that are able to kill bacteria growing as biofilms. 相似文献
179.
180.
本文研究了新疆列当科2属23种植物,用检索表列出了它们的不同,总结了前人的工作,并对它们的分布、生境、寄主给予了描述。 相似文献