全文获取类型
收费全文 | 44249篇 |
免费 | 2389篇 |
国内免费 | 4308篇 |
专业分类
林业 | 4106篇 |
农学 | 4895篇 |
基础科学 | 3312篇 |
5844篇 | |
综合类 | 17249篇 |
农作物 | 2871篇 |
水产渔业 | 1951篇 |
畜牧兽医 | 6102篇 |
园艺 | 2057篇 |
植物保护 | 2559篇 |
出版年
2024年 | 232篇 |
2023年 | 626篇 |
2022年 | 1539篇 |
2021年 | 1842篇 |
2020年 | 1707篇 |
2019年 | 1689篇 |
2018年 | 1165篇 |
2017年 | 1963篇 |
2016年 | 1528篇 |
2015年 | 2228篇 |
2014年 | 2221篇 |
2013年 | 2579篇 |
2012年 | 3456篇 |
2011年 | 3820篇 |
2010年 | 3442篇 |
2009年 | 3046篇 |
2008年 | 2948篇 |
2007年 | 2689篇 |
2006年 | 2161篇 |
2005年 | 1742篇 |
2004年 | 983篇 |
2003年 | 855篇 |
2002年 | 976篇 |
2001年 | 927篇 |
2000年 | 820篇 |
1999年 | 667篇 |
1998年 | 495篇 |
1997年 | 407篇 |
1996年 | 326篇 |
1995年 | 299篇 |
1994年 | 294篇 |
1993年 | 251篇 |
1992年 | 208篇 |
1991年 | 189篇 |
1990年 | 177篇 |
1989年 | 151篇 |
1988年 | 83篇 |
1987年 | 58篇 |
1986年 | 37篇 |
1985年 | 20篇 |
1984年 | 21篇 |
1983年 | 11篇 |
1982年 | 13篇 |
1981年 | 11篇 |
1980年 | 4篇 |
1979年 | 9篇 |
1964年 | 5篇 |
1963年 | 4篇 |
1962年 | 5篇 |
1956年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 812 毫秒
861.
862.
海洋贝类提取物对血管平滑肌细胞增殖的影响 总被引:2,自引:0,他引:2
采用20%胎牛血清(FBS),碱性成纤维细胞生长因子(bFGF)所诱导的大鼠VSMCs增殖模型,观察了海洋贝类提取物(ESs)对血管平滑肌细胞(VSMCs)增殖的影响。将培养的5-8代细胞接种于96孔板,除正常组加入含0.4%FBS的M199培养液外,其他组均加入含20%FBS培养液(或含50ng/ml bFGF的0.4%FBS培养液),同时给药组分别加入含有25,50,100μl/ml浓度的ESs,孵育48h后,以细胞计数,结晶紫染色及MTT比色法VSMCc生长情况。结果表明,与模型组比较,ESs各剂量组VSMCs的增殖受到明显抑制(P<0.05,P<0.01)。ESs对20%FBS和bFGF所致的VSMCs增殖具有明显抑制作用,并存在剂量依赖关系。 相似文献
863.
The ontogenetic development of the digestive enzymes amylase, lipase, trypsin, and alkaline phosphatase and the effect of
starvation in miiuy croaker Miichthys miiuy larvae were studied. The activities of these enzymes were detected prior to exogenous feeding, but their developmental patterns
differed remarkably. Trypsin activity continuously increased from 2 days after hatching (dah), peaked on 20 dah, and decreased
to 25 dah at weaning. Alkaline phosphatase activity oscillated at low levels within a small range after the first feeding
on 3 dah. In contrast, amylase and lipase activities followed the general developmental pattern that has been characterized
in fish larvae, with a succession of increases or decreases. Amylase, lipase, and trypsin activities generally started to
increase or decrease at transitions from endogenous to exogenous feeding or diet changes, suggesting that these enzymatic
activities can be modulated by feeding modes. The activities of all the enzymes remained stable from 25 dah onwards, coinciding
with the formation of gastric glands and pyloric caecum. These results imply that specific activities of these enzymes underwent
changes due to morphological and physiological modifications or diet shift during larval development but that they became
stable after the development of the digestive organs and associated glands was fully completed and the organs/glands functioned.
Trypsin and alkaline phosphatase were more sensitive to starvation than amylase and lipase because delayed feeding up to 2 days
after mouth opening was able to adversely affect their activities. Enzyme activities did not significantly differ among feeding
groups during endogenous feeding; however, all activities were remarkably reduced when delayed feeding was within 3 days
after mouth opening. Initiation of larvae feeding should occur within 2 days after mouth opening so that good growth and survival
can be obtained in the culture. 相似文献
864.
865.
水温13.8~20.0℃下,在容积50 L的塑料水槽中,放养体质量为(1.4±0.6)g的虾夷马粪海胆(Strongylocentrotus intermedius)11个,过量投喂海带(Laminaria japonica),再分别混养体质量为(3.4±0.6)g的仿刺参(Apostichopusjaponicus)0(A组)、3(B组)、5(C组)和10个(D组),体质量为(12.4±1.7)g的菲律宾蛤仔(Ruditapes philippim2rum)6个,排出水培养底栖硅藻和石纯(Ulva lactuca),用底栖硅藻饲喂仿刺参.77 d的饲养表明,仿刺参和海胆的成活率差异不显著(P>0.05),但混养组海胆的特定生长率(SGR)显著高于单养组(P<0.05),B、C和D组海胆的SGR分别比单养高9.12%、7.24%和10.06%,各混养组间差异不显著(P>0.05);混养海胆的饲料系数(7.28~7.70)分别显著低于单养(9.12)(P<0.05)组20.2%、15.5%和18.0%.将刺参的产量计算在内,B、C和D组海胆的饲料系数分别比对照组降低31.5%、26.8%和16.0%,但混养组间差异不显著(P>0.05).海胆和仿刺参的适宜混养比例为11:3.蛤仔和石纯生长慢,死亡率高.养殖排水培养的底栖硅藻以菱形藻(Nitzschia sp.)和卵形藻(Cocameissp.)为主,少量舟形藻(Navicula sp.).混养海胆性腺中亚麻酸、EPA DHA含量和n-3/n-6比值显著大于单养组(P<0.05),混养池水中氨氮含量低而稳,溶氧量高. 相似文献
866.
菲律宾蛤仔对镉、铜暴露的蓄积作用及其抗氧化酶系统的响应研究 总被引:1,自引:0,他引:1
研究了镉(Cd)、铜(Cu)2种重金属单一暴露下菲律宾蛤仔(Ruditapes philippinarum)肝胰腺和鳃组织内的重金属富集特征和抗氧化酶活力变化特点。结果表明,蛤仔肝胰腺与鳃对Cd、Cu的富集量随重金属浓度与暴靠时间的增加而增火,具有明显的浓度效应与时间效应;菲律宾蛤仔对Cd的富集能力高于Cu,H.肝胰腺中审盖属的蓄积量高于鳃;蛤仔肝胰腺和鳃组织中超氧化物歧化酶(SOD)、谷胱廿肽过氧化物酶((;Px)和谷胱竹肽硫转移酶(CST)活力在暴露的某些时段被显著诱导(P〈0.05),似随暴露时问延长或重金属浓度增加,SODf11GST活力则被显著抑制(P〈O.05),而CPx活力随暴露时问延K逐渐恢复至对照组水平、菲律宾蛤仔肝胰腺和鳃2种组织中SOD和GST的活性变化可作为海洋Cu^2+Cd^2+钙染的牛物标志物. 相似文献
867.
高寒鲤染色体核型分析 总被引:3,自引:2,他引:3
采用植物血球凝集素(PHA)体内注射,肾组织细胞短期培养,常规空气干燥法制备了高寒鲤染色体,核型分析结果是:高寒鲤染色体的核型公式为2n=100=28m+28sm+44stt,染色体臂数NF=156。 相似文献
868.
869.
Wenjia Li Fengxian Wei Bin Xu Shuqi Wang Shaoyu Li Xiongzhi Xuan 《Aquaculture Nutrition》2020,26(4):1289-1294
An 8‐week experiment was conducted to determine the optimal dietary lysine requirement for juvenile Protonibea diacanthus. Six isonitrogenous and isolipidic diets were formulated to contain levels of 10.8 (L10.8), 18.2 (L18.2), 26.1 (L26.1), 33.9 (L33.9), 40.7 (L40.7) and 48.6 g/kg (L48.6) of diets and were fed to the juvenile Protonibea diacanthus, respectively. The results indicated that weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and final weight (FW) increased as the dietary lysine level increased from 10.8 to 26.1 g/kg and then decreased as the dietary lysine levels further increased (p < .05). The lowest feed conversion ratio (FCR) was found when dietary lysine level was 26.1 g/kg. Analysis of specific growth rate by two slope broken‐line model indicated that the estimated optimal dietary levels of lysine for juvenile Protonibea diacanthus was 23.06 g/kg (51.24 g/kg dietary protein). 相似文献
870.