农村土地房屋资产管理研究进展

随着城镇化进程的推进,农村土地房屋流转在现实生活中日趋频繁,农村土地房屋资产管理问题进一步凸显。笔者综合运用文献资料法、对比分析法,围绕加强农村土地房屋资产管理的必要性、农村土地房屋资产管理面临的突出问题以及开展农村土地房屋资产管理创新探索3个命题,对学术界的研究成果进行梳理汇总,揭示了相关学术研究取得的成果、存在的主要问题,并展望了国内农村土地房屋资产管理研究的方向与重点。研究提出加强农村土地房屋资产管理,应当强化农村土地房屋资产管理主体、开展农村土地房屋资产清产核资、建立农村土地房屋资产收益分配机制、建立农村土地房屋资产评估服务体系、培育农村土地房屋资产市场。     

水稻ARF基因家族新成员ADP-Ribosylation Factor-Like Protein 5(OsARFL5)的克隆及表达分析

小G蛋白家族中的亚家族ADP-核糖基化因子(ADP-ribosylation factor, ARF),在生物体内起众多的生理作用,参与基因表达、细胞骨架重组装、微管的形成以及囊泡和核孔运输等。本研究已成功克隆得到ARF基因家族中的一个新基因OsARFL5,系统进化分析表明该基因与SiARFC1亲缘关系较近;以该基因和其启动子序列构建了两种遗传转化表达载体(pOsARFL5::GUS, pCaMV35S::OsARFL5:EGFP),并获得了相应遗传载体的转基因植株;GUS染色结果表明,该基因在不同时期、不同组织部位表达;洋葱表皮亚细胞定位结果显示,Os ARFL5基因编码的蛋白定位在细胞核。RT-PCR结果显示,OsARFL5在水稻不同生育期的根、茎、叶、叶鞘和穗子中均有不同程度的表达。本研究初步探明了ARF基因家族新成员OsARFL5在水稻生长发育过程中的表达模式。     

Development and validation of an indirect competitive enzyme-linked immunosorbent assay for the screening of tylosin and tilmicosin in muscle, liver, milk, honey and eggs

Incorrect use of tylosin and tilmicosin could result in allergy and select resistance. To monitor the illegal use of these antibiotics in animals, a monoclonal-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been established. Several haptens were synthesized and conjugated to carrier protein. Female Balb/c mice were inoculated with the four different conjugates to produce monoclonal antibodies according to the schemes of immunization. Aftercell fusion and culture several times, nine hybridoma cell lines were isolated. Only one, 3C4 that has isotype IgG2a, was selected for detailed study. The cross-reactivity of the monoclonal antibody 3C4 to tylosin and tilmicosin was 100% and 51% respectively. The standard curves based on the tylosin and tilmicosin matrix calibration ranged from 2.5 to 40 μg L(-1), with an IC(50) value of 6.1 μg L(-1) and 12.1 μg L(-1), respectively. The limits of detection of the ic-ELISA ranged from 5.1 μg kg(-1) to 13.8 μg kg(-1) in edible animal tissues. The recoveries were 74.1% to 120.7% with less than 18.6% of the coefficient of variation when tylosin and tilmicosin were spiked in various biological matrices with the concentrations of 25.0-200.0 μg kg(-1). Good correlations between the results of the ic-ELISA and high performance liquid chromatography were observed in the incurred tissues. These results suggest that the ic-ELISA is a sensitive, accurate and low-cost method that would be a useful tool for the screening of the residues of tylosin and tilmicosin in muscle, liver, milk, honey and eggs.     

农用土地经济定级中两种方法的比较研究——以新疆一四八农场为例

以新疆一四八农场为例，对农用土地经济定级中的模拟作物产量法和综合土地质量法及其结果进行了比较，结果表明：（1）两种方法定级程序基本一致，在地方性区域内的定级结果均较为准确，且一致性较大；（2）模拟作物产量法结果的区域可比性强，但方法复杂；（3）综合土 地质量法方法简便易行，但结果的区域可比性差；（4）两种方法结合，能有效地建立便于比较和纳入全国体系的农用土地经济等级指数。     

Lipophilic hydroxytyrosyl esters. Antioxidant activity in lipid matrices and biological systems

Antioxidant activities of lipophilic hydroxytyrosyl acetate, palmitate, oleate, and linoleate were compared with those of hydroxytyrosol, alpha-tocopherol, and butylhydroxytoluene (BHT) in both glyceridic matrix and biological systems. Aliquots of a glyceridic matrix spiked with various concentrations of antioxidant were subjected to accelerated oxidation in a Rancimat apparatus operated at 90 degrees C. The relationships between induction time (IT) and antioxidant concentration (mmol/kg) presented by hydroxytyrosol and hydroxytyrosyl acetate, palmitate, oleate, and linoleate were similar. Hydroxytyrosol and its esters showed greater antioxidant activity than alpha-tocopherol or BHT. We also evaluated the capacity of hydroxytyrosyl esters to protect proteins and lipids against oxidation caused by peroxyl radicals, using a brain homogenate as an ex vivo model. All tested compounds showed a protective effect in these systems, which was greater in preventing the generation of carbonyl groups in protein than of malondialdehyde in lipid. Inclusion of a lipophilic chain in the hydroxytyrosol molecule enhanced its antioxidant capacities in this biological model.     

面向对象的湖北省土地覆被变化遥感快速监测

为了快速监测湖北省土地覆被变化,以HJ-CCD、TM以及ETM影像为数据源,探索出了集成面向对象技术与"3S"技术的中尺度土地覆被变化遥感流程和方法。该方法首先基于HJ-CCD影像和面向对象技术进行2010年湖北省土地覆被信息的提取,并用野外采样点对结果进行验证。在保证高精度的情况下利用向量相似性函数对湖北省2000－2005年以及2005－2010年进行变化检测,从而获得2个变化时期影像的变化区域,然后利用面向对象技术中的最邻近分类器对变化区进行自动化分类。最后分别将2期的变化结果与未变化结果融合后获得湖北省2000年、2005年和2010年的土地覆被图,在GIS中处理后建成县、市、省3级行政级别的湖北省土地覆被变化本底数据库。试验表明分类结果的总体精度为93.24%,Kappa系数为0.914;2000－2005年与2005－2010年变化检测的总体精度分别为90.88%和90.75%。同时研究发现景观破碎的地区应用面向对象技术有一定的局限性。     

钢桁架混凝土梁试验研究

The present paper studies the applicability of composite concrete beams to building and bridge structures. The ultimate flexural and shearing strength, the flexural stiffness, and the width of cracks of a series of composite concrete beams are analysed an     

离子束生物技术在药用植物遗传改良中的应用前景

本文主要简要介绍了离子束生物工程的特点,系统阐述了药用植物资源领域的现状以及离子束作为一种新的诱变源在药用植物遗传改良中的应用前景。     

蒜头果叶绿体基因组密码子偏好性分析

为了解蒜头果叶绿体基因组密码子的使用偏性,利用Codon W 1.4.2和CUSP软件对蒜头果叶绿体基因组中33个基因的密码子进行中性绘图,应用ENC−plot、PR2−plot绘图分析并确定了其最优密码子。结果表明：蒜头果叶绿体基因组密码子的第3位碱基GC含量为28.43%,远低于第1位（47.74%）和第2位（41.05%）,RSCU值大于1的密码子有30个,其中12个以A结尾,16个以U结尾,说明蒜头果叶绿体基因组的编码基因偏好以A和U结尾。GC₁₂和GC₃的相关系数为0.1646,相关性不显著,回归系数为0.0004;有效密码子数（ENC）范围为40.39～54.86,大于45的有25个,ENC比值主要分布在−0.05～0.05之外。蒜头果叶绿体基因组中的大部分基因分布在PR2平面图的下半部或右下半部,说明蒜头果叶绿体基因组密码子偏好性更多地受选择的影响,同时亦受其他因素的影响。蒜头果叶绿体基因组的最优密码子确定为UUU、UUA、GUA等18个。     

光照强度对冬小麦旗叶光合生理特性的影响

为建立冬小麦相应的光合作用模型提供数据支持,在20℃、不同光强梯度下,测定了冬小麦旗叶净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、胞间CO2浓度(Ci)等生理指标,研究自然条件下抽穗期冬小麦旗叶光合生理特性的变化规律。结果表明,光合速率随光照强度增加而增加并呈正相关(p<0.05),在光强度低于500μmol/(m2.s)时,小麦旗叶的光合速率快速增加;气孔导度随光强增强而增强并呈正相关(p<0.05),但在光强度低于100μmol/(m2.s)时气孔导度增幅很小;蒸腾速率随光强度的变化与光合速率趋势基本一致;胞间CO2浓度随光强度的增加呈先降低后增加趋势。