Characterization of monoclonal antibodies against avian reovirus S1133 protein sigmaA synthesized in Escherichia coli

Monoclonal antibodies (MAbs) were prepared against avian reovirus S1133 protein sigmaA (esigmaA) synthesized in Escherichia coli. MAbs were characterized and used to develop a diagnostic test. Ten MAbs were selected for competitive binding assay following coupling with horseradish peroxidase. The results indicated that these MAbs delineated two epitopes I and II of esigmaA. An immuno-dot binding assay was used to detect the effect of denaturation on antibody recognition of the epitopes. All MAbs bound to esigmaA in its native form. After denaturation by boiling in SDS and 2-mercaptoethanol, the binding of MAbs recognizing epitope I was fully abolished. However, the reactivity of MAbs recognizing epitope II was not affected. MAbs 31 and 32, recognizing epitopes I and II, respectively, were selected for the cross-reactivity to heterologous reovirus strains. The results suggest that the two epitopes are highly conserved among these virus strains. A MAb capture enzyme-linked immunosorbent assay (ELISA) procedure was developed using MAbs 32 and 31 to detect reovirus protein sigmaA in samples from tendon tissues of infected bird and chicken embryo fibroblast (CEF) cell cultures. Avian reovirus sigmaA antigens in tendon specimens were detected from the inoculated birds as early as 2 days post-inoculation (PI), approximated a peak at 7 days PI, and maintained this until 16 days PI, then decreased gradually. A clear difference in absorbance values between the tendon samples of the avian reovirus- and mock-infected birds is obtained. Positive results were also obtained from avian reovirus-infected CEF and from the tendon tissues of naturally infected broilers. These results indicated that the MAb capture ELISA is a useful methods for the detection of avian reovirus from chickens suspected to have avian reovirus infections.     

溶菌酶在食品工业中的应用

本文综述了溶菌酶的基本性质，着重讨论了溶菌酶抑制的微生物种类、机理，系统的阐述了溶菌酶的制备和在食品工业生产过程中应用的进展情况。     

双重排水条件下控制措施对银南灌区水稻田水盐关系的影响分析

针对宁夏银南灌区目前存在的排水过量问题,研究者提出了利用控制排水措施,在适当时段减少排水量的建议。然而,灌区在农沟以及更深层的支、干沟及黄河(低地)的影响下,受着深层排水和浅层排水的双重作用。其中深层排水一般不会受到田间措施的影响,它的存在“淡化或中和”了由于浅层排水的减少引起的田间含盐量的增加。本文在对双重排水情况下的水盐运动进行概化的基础上,建立了基于特定地区排水特性的“浅层排水与深层排水比-浅层排水控制率-排水含盐量增加率”的关系。最后结合根据地下水洗淋的临界情况,确定了达到这一目标控制指标以及浅层地下水控制率。结果显示,对于研究的典型情况,当地下水的盐分含量达到临界的1.62 g/l时,可以将1m深的农沟控制至30cm深;这时的浅层地下水仅为现状的14%,即从731mm减少到了102mm。     

鸭副黏病毒的分离与鉴定

从广东省某鸭场的病死番鸭肝脏中分离一株病毒,该病毒对雏鸭、种鸭、蛋鸭均可引起发病。接种鸭胚传至第6代时病毒对鸡红细胞的血凝性稳定,通常在72h开始致死非免疫鸭胚,死亡率为80%。经磷钨酸负染电镜观察,病毒粒子多呈现椭圆形,长轴为167～264nm,短轴为131～139nm,病毒外表有囊膜,囊膜外层有排列整齐的纤突。病毒与禽流感病毒无抗原相关性,能被禽副黏病毒I型阳性血清所中和。根据试验结果,初步将分离的病毒判定为鸭副黏病毒。     

Guided bone regeneration with beta-tricalcium phosphate and poly L-lactide-co-glycolide-co-epsilon-caprolactone membrane in partial defects of canine humerus

This study was performed to evaluate the effect of beta-tricalcium phosphate and poly L-lactide-co-glycolide-co-epsilon-caprolactone (TCP/PLGC) membrane in the repair of partial bone defects in canine proximal humerus. Three adult mixed-breed dogs were used during the experimental period. The length of the defect was quarter of the full length of humerus, and width of the defect was quarter of middle diameter of the lateral aspect of humerus. The humeri of each dog were divided into treatment (TCP/PLGC) and control groups. The defect was covered with TCP/PLGC membrane in treatment group. To evaluate regeneration of the bone, computerized tomography (CT) and histopathologic examination were performed. The radiopaque lines were appeared at the original defect sites in TCP/PLGC group but below the original site in control at 4th week. Radiopacity and thickness of the defect sites, and radiopaque lines were more increased at 8th week than those of 4th week. Histopathologic findings revealed fibrous connective tissue migration into the defect and the migration inhibited the structure of new cortex to be placed in the original level in control whereas new cortex growth was found in the level of original line in TCP/PLGC group. However, the new cortical bone in the TCP/PLGC group was thinner and less organized than the adjacent intact cortex, and the amount of new cancellous bones were also scanty. The result suggested that TCP/PLGC membrane is a good guided bone regeneration material to restore the original morphology of humerus in partial defect.     

水杨酸与蔬菜作物抗性关系的研究进展

水杨酸在植物的抗病性方面发挥着重要的作用,近年来的研究表明,水杨酸在植物抗冷、热、盐、干旱、臭氧、重金属等环境胁迫方面有明显的作用。对近年来有关水杨酸在蔬菜抗逆境胁迫方面的研究进展进行综述,并提出一些研究思路。     

日本《外来入侵物种法》及对我国外来物种管理立法和科研的启示

日本政府于2004年6月颁布了《外来入侵物种法》,10月环境省制定的《预防外来入侵生物对生态系统造成不利影响的基本政策》通过内阁决议,该法案于2005年6月1日起生效。本文简要介绍了日本外来生物管理有关策略和基本政策,并针对我国现状提出了外来物种管理立法及科研方面的建议:(1)成立国家级外来物种管理委员会及外来物种专家委员会,统筹外来物种管理工作;(2)对现有法律法规体系进行系统评估,加强外来物种管理立法;(3)加强对外来物种的科学研究,特别是加强外来入侵物种环境风险评估系统建设;(4)进一步加强建立国家级、一站式的外来物种数据库和外来物种数据交换所,促进信息交流。     

椰心叶甲饥饿耐受性研究

研究了椰心叶甲的饥饿耐受时间,饥饿状态下的化蛹率和羽化率.结果表明常温下,椰心叶甲幼虫阶段的饥饿耐受时间从长到短分别为5龄幼虫、4龄幼虫、3龄幼虫、2龄幼虫、1龄幼虫,随发育历期的延长饥饿耐受时间逐渐增加.雌成虫的饥饿耐受时间较雄成虫长.饥饿状态下,椰心叶甲幼虫的化蛹率和羽化率随发育历期的延长而逐渐增加.     

Gross Anatomy of the Pancreatic Lobes and Ducts in Six Breeds of Domestic Ducks and Six Species of Wild Ducks in China

A previously unreported pancreatic duct was found by Liu (1989) in Pekin ducks. This duct has now been consistently found in six breeds of domestic ducks and six species of wild ducks in China. For purposes of Nomina Anatomica Avium it is hereby called the ‘first pancreatic duct’(Ducius pancreaticus primus) since it enters the duodenum at or near the flexure where the descending duodenum becomes the ascending duodenum. All other pancreatic ducts enter the duodenum later, closer to where it joins the jejunum. This first pancreatic duct drains the caudal extremity of the dorsal lobe of the pancreas and can be easily exteriorized for experimental purposes. Within the parenchyma of the dorsal lobe of the pancreas this duct communicates with the dorsal pancreatic duct. In the present study of the gross anatomy of the pancreatic lobes of domestic and wild Chinese ducks we describe and illustrate variations in position and number of all biliary and pancreatic ducts.     

麦套花生优化施肥研究

采用三因素二次饱和Ｄ—最优设计，建立了麦套花生Ｎ、Ｐ、Ｋ施肥与花生产量间的数学模型。由模型可得：（１）Ｎ、Ｐ、Ｋ三种肥料对麦套花生产量作用显著，且互作间存在较大的交互效应，因此，Ｎ、Ｐ、Ｋ需配合使用。（２）公顷产量４５００～５２５０ｋｇ的优化施肥方案为：每公顷施Ｎ１０９．５～１４７ｋｇ，Ｐ２Ｏ５１３３．５～１８６ｋｇ，Ｋ２Ｏ２４３～３４８ｋｇ；最优组合为：每公顷施Ｎ１３９．６ｋｇ，Ｐ２Ｏ５１７４．３ｋｇ和Ｋ２Ｏ３２３．３ｋｇ，最高产量可达５２６０ｋｇ／ｈｍ２。