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排序方式: 共有249条查询结果,搜索用时 265 毫秒
91.
92.
P. Nagarajan P. D. Walton 《The Journal of Horticultural Science and Biotechnology》2013,88(3):443-450
SummaryThe effects of ε-aminocaproic acid (EACA) on self-incompatibility was tested in a Phaseolus coccineus line which failed to form pods in controlled pollinations. Scanning electron microscope (SEM) and fluorescence miscroscopy confirmed problems in pollen-germination and pollen-tube growth but EACA-treated self- pollinations resulted in fairly normal pollen germination and pollen tube growth. In a few cases, pollen tubes were seen entering the embryo sac and occasionally pods were formed. Incongruity barriers also exist in crosses of P. coccineus × P. vulgaris, P. acutifolius × P. coccineus and their reciprocals. Effects of EACA on flower abscission, pod development and pod abscission were studied. Increased pod formation was observed in EACA treated materials, except in P. acutifolius × P. coccineus and P. coccineus × P. vulgaris cv. Jacobs Cattle. EACA seems to act at the stigmatic and stylar levels, thereby enhancing pollen germination and pollen tube growth and delaying flower abscission. The net result is fertilization and delayed senescence which permit the pod to grow for a longer period of time. 相似文献
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96.
VanLeeuwen JA Mellish T Walton C Kaniaru A Gitau R Mellish K Maina B Wichtel J 《Tropical animal health and production》2012,44(2):231-238
We aimed to describe the management and productivity of this group of smallholder dairy farmers in Kenya at the beginning
of an intervention program and to document relevant observed changes in the 3rd year of the intervention. A 3-year intervention
program, focused on management of animal health and nutrition, and milk quality, was implemented by one Kenyan and two Canadian
non-governmental organizations (one university based) to help improve the milk production and livelihoods of Kenyan smallholder
dairy farmers (primarily women). Thirty farmers were enrolled and completed questionnaires at the start and end of the project.
Focus groups were also conducted to obtain qualitative information on livelihood effects from the program. In 2004, 70% of
the eligible youngstock (more than 15 months of age) were pregnant, and cows had a long average days-in-milk of 240 days.
External parasites, poor hygiene, and long claws were not uncommon among cows, and 37% and 20% of the farms reported clinical
intestinal parasitism and diarrhea in youngstock. In 2006, there were significant improvements in the proportion of farms
planting high-protein forages, farms using better milking procedures, and on-farm milk storage methods. The reported mastitis
incidence rate fell from 0.55 to 0.20 cases/cow-year (p < 0.01), and the average number of cows and youngstock significantly increased from 1.5 and 0.9 to 2.9 and 2.6, respectively.
There were reported improvements in the livelihoods among the member families. The partnership-based intervention program
significantly improved management and productivity of this group of smallholder dairy farmers in rural Kenya, leading to reported
livelihood benefits. 相似文献
97.
Walton RM 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(2):175-181
Subject-based reference values have been largely overlooked in veterinary medicine. These values represent longitudinal data rather than the cross-sectional data represented by standard population-based reference values. As such they provide information about biological and analytical variation. Inherent random variation of analytes around a homeostatic set point is referred to as biological variation; data on biological variation are underutilized in veterinary medicine and have multiple applications that include setting analytical goals, predicting the utility of population-based reference intervals (RIs), assessing the value of partitioning reference values, and evaluating the significance of changes in serial results. To generate these data, relatively few individuals are sampled for a short period of time. Given the difficulty of obtaining specimens from large number of healthy individuals to establish a cross-sectional RI for many veterinary species, especially exotic species, use of subject-based RIs is a practical alternative approach for the veterinary diagnostician. Furthermore, for the majority of biochemical analytes and even many hemostatic variables, population-based reference values are less sensitive than subject-based reference values for detecting pathologic changes in an individual. The focus of this review is the clinical usefulness of subject-based reference values and diagnostic implications for their use. Implementation of the concepts of biological variation, individuality, and reference change value (RCV) may allow large diagnostic laboratories to offer more sensitive reference values to assess health and detect disease. 相似文献
98.
Jintana Yanola Catherine Walton La-aied Prapanthadara 《Pesticide biochemistry and physiology》2010,96(3):127-131
Sodium channel mutations were investigated through nucleotide sequencing of three cDNA fragments amplified from permethrin resistant and susceptible Aedes aegypti from northern Thailand. There was a novel nucleotide substitution (T → G) at the second position of codon 1552 resulting in the replacement of Phenylalanine by Cysteine in segment 6 domain III. This amino acid was indicated by another study to involve an aromatic-aromatic contact between the sodium channel protein and the first aromatic ring of the pyrethroid alcohol moiety. Reciprocal crosses between the homozygous parental susceptible and resistant strains indicated that resistance was autosomal and incompletely recessive, and highly associated with the homozygous mutation. The bioassay of the F2 progeny, formed by backcrossing the F1 with the resistant parental strain, did not show a clear plateau curve across the range of doses, suggesting that resistance to permethrin was controlled by more than one gene locus. Other possible resistance mechanisms involved are discussed. 相似文献
99.
Annette C. Richardson Eric F. Walton John S. Meekings Helen L. Boldingh 《Scientia Horticulturae》2010
Carbohydrates were measured in axillary bud meristems from kiwifruit (Actinidia deliciosa (A.Chev.) C.F. Liang et A.R. Ferguson cultivar Hayward) to determine whether concentrations changed during winter dormancy. Buds were collected from kiwifruit vines growing in four sites, which spanned the climatic range that kiwifruit are grown in New Zealand, and from vines where the time of budbreak had been manipulated using hydrogen cyanamide. During winter, sugars comprised 78% of measured carbohydrates in meristems, with sucrose accounting for more than 86% of sugars detected (up to 200 mg g DW−1). Starch concentrations in bud meristems showed little change during winter. Meristem sucrose and hexose concentrations increased rapidly from autumn until mid-winter, and this was correlated with leaf abscission. Sugar accumulation ceased in mid-winter and concentrations remained stable until spring, suggesting that bud meristem activity was low. Four weeks before visible bud movement, a marked decline in sucrose and increase in hexose concentrations, indicated activity in the meristems had increased prior to budbreak. The patterns of seasonal change and peak sucrose concentrations in meristems were similar at all sites. The only difference was in the timing of events, with changes in meristem sugars occurring earliest in vines growing at the coolest site, coinciding with their earlier cessation of and resumption of vine growth. Advancing budbreak, by applying hydrogen cyanamide to vines in late winter, also advanced changes in sugar concentrations within meristems. This work suggests that the concentration of sucrose in kiwifruit bud meristems has utility as an indicator of whether they are being induced into, maintained or released from winter dormancy. 相似文献
100.
P C Owens K L Gatford P E Walton W Morley R G Campbell 《Journal of animal science》1999,77(8):2098-2103
Previous studies have reported conflicting data on gender differences in plasma IGF-I in postnatal pigs. There is also debate over the role of IGF-II in regulation of postnatal growth. We have, therefore, determined the concentrations of plasma IGF-I, IGF-II, and IGF-binding protein-3 (IGFBP-3) in boars, barrows, and gilts and related these to postnatal growth characteristics. Plasma concentrations of IGF-I were higher in boars than in gilts or barrows from 13 wk. of age, and plasma IGF-II levels were generally higher in barrows than in boars or gilts. Plasma IGFBP-3 levels were higher in boars than in gilts or barrows at most ages. Between 15 and 23 wk. of age, IGF-I and IGFBP-3, but not IGF-II, were positively associated with growth rate, voluntary feed intake, and gain:feed ratio. Plasma IGF-II, but not IGF-I or IGFBP-3, was positively associated with backfat depth during this period. These results support the hypothesis that circulating IGF-I and IGF-II are regulators of lean and adipose tissue growth, respectively. 相似文献