全文获取类型
收费全文 | 258篇 |
免费 | 16篇 |
专业分类
林业 | 4篇 |
基础科学 | 1篇 |
11篇 | |
综合类 | 23篇 |
农作物 | 6篇 |
水产渔业 | 4篇 |
畜牧兽医 | 220篇 |
植物保护 | 5篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 9篇 |
2015年 | 2篇 |
2014年 | 7篇 |
2013年 | 21篇 |
2012年 | 4篇 |
2011年 | 9篇 |
2010年 | 4篇 |
2009年 | 7篇 |
2008年 | 6篇 |
2007年 | 16篇 |
2006年 | 13篇 |
2005年 | 13篇 |
2004年 | 13篇 |
2003年 | 17篇 |
2002年 | 5篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 10篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 9篇 |
1990年 | 3篇 |
1989年 | 7篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1983年 | 4篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 2篇 |
1968年 | 1篇 |
1967年 | 2篇 |
1945年 | 1篇 |
1944年 | 3篇 |
1942年 | 1篇 |
1926年 | 1篇 |
1925年 | 2篇 |
排序方式: 共有274条查询结果,搜索用时 15 毫秒
91.
92.
Abstract AIM: To investigate polymeric nanoparticles as an oral delivery system for protein biocontrol agents for control of the brushtail possum, Trichosurus vulpecula. METHODS: Insulin-loaded poly(ethyl 2-cyanoacrylate) (PECA) nanoparticles were prepared using interfacial polymerisation, and characterised for size, zeta potential, and efficiency of encapsulation of insulin. In-vitro release of insulin-loaded PECA nanoparticles was quantified using reverse-phase highpressure liquid chromatography (HPLC). The in-vivo pharmacokinetics of insulin in PECA nanoparticles was investigated following I/V administration, and when injected directly into the caecum alone or in conjunction with the permeation enhancer EDTA. Blood samples were collected at intervals from ?5 to 180 minutes, and the concentration of insulin in plasma was quantified using a radioimmunoassay (RIA) validated for possum plasma. RESULTS: Poly(ethyl 2-cyanoacrylate) nanoparticles were produced with a uniform particle size of 200–300 nm, and the mean entrapment of insulin was 78%. In-vitro release of insulin from the PECA nanoparticles was controlled, although incomplete, and approximately 30% of the insulin remained entrapped. The bioavailability of insulin when administered in a PECA nanoparticulate formulation injected directly into the caecum was <1%, and was not increased by addition of the permeation enhancer. CONCLUSIONS: The nanoparticulate formulations investigated as part of this study resulted in low bioavailability of the peptide insulin in the brushtail possum. 相似文献
93.
94.
95.
COMPARISON OF TRANSVERSE COMPUTED TOMOGRAPHIC EXCRETORY UROGRAPHY IMAGES AND MAXIMUM INTENSITY PROJECTION IMAGES FOR DIAGNOSING ECTOPIC URETERS IN DOGS
下载免费PDF全文
![点击此处可从《Veterinary radiology & ultrasound》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Scott Secrest Andrew Bugbee Kenneth Waller David A. Jiménez 《Veterinary radiology & ultrasound》2017,58(2):163-168
Computed tomographic maximum intensity projection (MIP) images have been shown to improve reader confidence in their diagnoses and to improve detection of vascular structures and pulmonary nodules. The objectives of this method comparison study were to compare transverse source computed tomographic excretory urography (CTEU) images to two, five, and 10 slab thick MIP images for diagnosing canine ectopic ureters, compare reader confidence, and evaluate interobserver agreement. Two board‐certified veterinary radiologists and a board‐certified small animal internist blindly reviewed transverse source CTEU and two, five, and 10 slab thick MIP images of 24 dogs enrolled in the study. Inclusion criteria included a diagnostic CTEU and either cystoscopic or surgical confirmation of the presence or absence of ureteral ectopia. Eleven dogs were confirmed to have 17 ectopic ureters at surgery and/or cystoscopically. There was no significant difference in reader diagnoses between viewing methods or between viewing methods and the surgical/cystoscopic findings (P < 0.001). Reader confidence was significantly greater on two (P = 0.0080) and five (P = 0.0009) slab thick MIP images with significant interobserver agreement between readers for all viewing methods (P values ranging between 0.0363 and <0.001). In addition, the diagnostic accuracy of MIP images was similar to or better than transverse source CTEU images, when assessed by a radiologist. The study results suggest that CTEU is a reliable imaging technique for diagnosing canine ectopic ureters among specialists of varied experience. In addition, thin slice reconstructed MIP images improve reader confidence and potentially diagnostic accuracy, and thus their use should be considered, especially in more challenging cases. 相似文献
96.
The structure of the upper atmosphere of mars: In situ accelerometer measurements from mars global surveyor 总被引:1,自引:0,他引:1
GM Keating SW Bougher RW Zurek RH Tolson GJ Cancro SN Noll JS Parker TJ Schellenberg RW Shane BL Wilkerson JR Murphy JL Hollingsworth RM Haberle M Joshi JC Pearl BJ Conrath MD Smith RT Clancy RC Blanchard RG Wilmoth DF Rault TZ Martin DT Lyons PB Esposito MD Johnston al et 《Science (New York, N.Y.)》1998,279(5357):1672-1676
The Mars Global Surveyor (MGS) z-axis accelerometer has obtained over 200 vertical structures of thermospheric density, temperature, and pressure, ranging from 110 to 170 kilometers, compared to only three previous such vertical structures. In November 1997, a regional dust storm in the Southern Hemisphere triggered an unexpectedly large thermospheric response at mid-northern latitudes, increasing the altitude of thermospheric pressure surfaces there by as much as 8 kilometers and indicating a strong global thermospheric response to a regional dust storm. Throughout the MGS mission, thermospheric density bulges have been detected on opposite sides of the planet near 90 degreesE and 90 degreesW, in the vicinity of maximum terrain heights. This wave 2 pattern may be caused by topographically-forced planetary waves propagating up from the lower atmosphere. 相似文献
97.
S S Trostle R T O'Brien J Britt K R Waller 《Journal of the American Veterinary Medical Association》1999,215(3):366-368
OBJECTIVE: To determine whether exogenous isobutane gas infused into the udders of dairy cattle could be detected ultrasonographically, and if so, what effects volume of gas infused and infusion pressure had on how long after infusion exogenous isobutane gas could be detected. DESIGN: Randomized block design. ANIMALS: 8 Holstein cows 28 to 32 days after parturition. PROCEDURE: In each cow, 1 mammary gland was not treated and the other 3 received 1 of 3 treatments by means of intramammary infusion: low volume-high pressure, low volume-low pressure, and high volume-high pressure infusion of isobutane gas. Mammary glands were examined ultrasonographically 1 hour before and 1, 3, 12, 24, 36, 48, 72, and 96 hours after treatment. RESULTS: After intramammary infusion of isobutane gas, bright echoes and associated acoustic shadows were seen ultrasonographically; echoes were no longer seen 72 hours after gas infusion. Percentages of mammary glands in which bright echoes were detected were not significantly different among the 3 treatment groups at any time during the study. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that exogenous isobutane gas infused into the mammary glands to enhance the appearance of the udder of show dairy cattle can be readily detected by ultrasonography. 相似文献
98.
The effect of experimental infectious mastitis on leukocyte subpopulations and cytokine production in non-lactating ewes. 总被引:2,自引:0,他引:2
K P Waller I G Colditz 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1999,46(5):289-299
The interactions between leukocytes and cytokines during the acute response to intramammary infections in the dry mammary gland of sheep were studied. Dry ewes were experimentally infected in one udder half with either Staphylococcus aureus or Escherichia coli, or infused with saline as control. Udder secretion samples, blood samples and udder tissue samples were collected before and 4, 8 and 24 h after infections/infusions. Total and differential leukocyte counts were calculated in both blood and mammary secretions, and flow cytometry was used to detect the presence of CD4+, CD8+, WC1+, IL-2R+, CD18+ or L-selectin + lymphocytes, CD18+ or L-selectin + neutrophils, and CD14+ leukocytes. Moreover, the concentrations of interleukin-1 beta (IL-1 beta), interleukin-8 (IL-8) and granulocyte-macrophage colony stimulating factor (GM-CSF) in udder secretions were measured using ELISA, and RT-PCR was used to detect the presence of corresponding cytokine mRNA in udder tissue biopsies. The results suggest an association between the concentrations of IL-1 beta, IL-8 and the intensity of neutrophil infiltration of the infected gland. Immunologically relevant changes in proportions of lymphocyte subpopulations might also occur in the acute phase of the inflammatory reaction of the udder. Greater cellular and cytokine responses to E. coli infection may have contributed to the milder clinical picture and more rapid resolution of infection than that seen for S. aureus. Enhancing the production of pro-inflammatory cytokines may improve defence against bacterial mastitis. 相似文献
99.
Adrian A. Bischoff Patrick Fink Uwe Waller 《Aquaculture (Amsterdam, Netherlands)》2009,296(3-4):271-276
The potential of solid waste originating from a recirculated fish culture system, i.e. faecal material, uneaten food pellets and bacterial biofilms was examined as food source for the marine polychaete Nereis diversicolor. These polychaetes could be a valuable food for fish if they provide essential fatty acids to the fish. Therefore, we analysed the fatty acid profiles from feed and faecal materials, the sediment as well as the cultured organisms — fish and several batches of N. diversicolor — from an integrated recirculating aquaculture system.The major fatty acids (saturated, monounsaturated and polyunsaturated) for all analysed fish feed, fish and faeces samples were C16:0, C18:1 and C22:6 (n − 3), accounting for 48% to 57% of the fatty acids in the samples. The major fatty acids within the sediment were C16:0, C18:1 and C18:3 (n − 3), accounting for 61% of the total fatty acids. The samples of N. diversicolor revealed C16:0, C18:1 and C20:5 (n − 3) as the major fatty acids. Combined, they accounted for 56% of the total fatty acids detected within the worm samples.The results indicate that a recycling or even an upgrade of excreted feed nutrients such as fatty acids, which were otherwise discharged, can be achieved through integrated aquaculture combining fish and worm culture. 相似文献
100.