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991.
Christopher R. Thornton 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(3):347-353
Species of the genus Trichoderma are ubiquitous soil-borne fungi that exhibit antagonism towards a number of economically important plant-pathogenic fungi
and oomycetes. This review discusses recent developments in the use of monoclonal antibodies to detect these fungi in their
natural soil environments and to quantify their population dynamics during antagonistic interactions with saprotrophic competitors
in soil-based systems. Immunological approaches to detection and quantification are examined in relation to conventional plate
enrichment techniques and to nucleic acid-based procedures. An example of recent research using a mAb-based assay to quantify
the effects of saprotrophic competition on the growth of Trichoderma isolates in mixed species, soil-based, microcosms is presented. Future technological developments in immunoassays for tracking
Trichoderma populations in soil are discussed and results presented showing the accurate detection and visualization of a plant growth-promoting
isolate of T. hamatum in the rhizosphere of lettuce using mAb-based immunodiagnostic assays. 相似文献
992.
Masashi Fujinaga Naho Yamagishi Hideki Ogiso Jun Takeuchi Jouji Moriwaki Toyozo Sato 《Journal of General Plant Pathology》2011,77(4):243-247
A new disease was found in Japan, on celery (Apium graveolens var. dulce) having severe chlorotic leaf spot, stunt, and dwarf with leaf curl. A spore suspension from the fungus isolated from affected
plants induced identical symptoms 14 days after plants were sprayed. Identification and molecular characterization showed
that the causal agent is Colletotrichum simmondsii. This report is the first of stunt anthracnose on celery caused by C. simmondsii. We propose the name “stunt anthracnose” for the new disease. Colletotrichum acutatum sensu lato, as reference pathogen of celery anthracnose, should be changed to C. fioriniae based on morphological and molecular characteristics. 相似文献
993.
Context
Human and natural systems interact at multiple scales which are context specific in relation to ecosystem service supply. Scenic beauty is recognised as a cultural ecosystem service whose aesthetic value is perceived at a holistic landscape level.Objectives
In this study we provide methodological advancements for assessing the relationship between landscape visual character and scenic beauty based on crowdsourced geographic information. The final aim is to demonstrate, through a case study application, an empirical method for mapping the scenic beauty of complex mountain landscapes from the perspective of observers which are realistically exposed to the environment being evaluated.Methods
We propose a viewshed based approach which relies on visual indicators and the location of visitors retrieved by public image storage analysis. A cluster analysis was used to integrate visual characters of the landscape and visiting users’ preferences.Results
Four different typologies of landscapes were finally characterized by distinct values of visual indicators. The spatial distribution of the landscape typologies presented a clustered pattern, allowing a regionalization of the landscape characters. The analysis of the visiting users’ provenance revealed that visual scale, naturalness and ephemera attract mainly foreign users, while imageability, complexity and historicity attract mostly domestic and local users.Conclusions
The combination of crowdsourced images with visual indicators allows a systematic analysis of landscape scenic beauty properties. In all, by understanding how specific landscape characters contributes to aesthetic service provision we provide a tool for facilitating the visualization and interpretation of complex landscape characters.994.
Luis J. Villanueva-Rivera Bryan C. Pijanowski Jarrod Doucette Burak Pekin 《Landscape Ecology》2011,26(9):1233-1246
In this paper we present an introduction to the physical characteristics of sound, basic recording principles as well as several
ways to analyze digital sound files using spectrogram analysis. This paper is designed to be a “primer” which we hope will
encourage landscape ecologists to study soundscapes. This primer uses data from a long-term study that are analyzed using
common software tools. The paper presents these analyses as exercises. Spectrogram analyses are presented here introducing
indices familiar to ecologists (e.g., Shannon’s diversity, evenness, dominance) and GIS experts (patch analysis). A supplemental
online tutorial provides detailed instructions with step by step directions for these exercises. We discuss specific terms
when working with digital sound analysis, comment on the state of the art in acoustic analysis and present recommendations
for future research. 相似文献
995.
The ecology and culture of landscape sustainability: emerging knowledge and innovation in landscape research and practice 总被引:3,自引:1,他引:3
Laura R. Musacchio 《Landscape Ecology》2009,24(8):989-992
Landscape researchers and practitioners, using the lens of sustainability science, are breaking new ground about how people’s
behaviors and actions influence the structure, function, and change of designed landscapes in an urbanizing world. The phrase—the
scientific basis of the design for landscape sustainability—is used to describe how sustainability science can contribute
to translational landscape research and practice about the systemic relationships among landscape sustainability, people’s
contact with nature, and complex place-based problems. In the first section of this article, important definitions about the
scientific basis of the design for landscape sustainability are reviewed including the six Es of landscape sustainability—environment, economic, equity, aesthetics, experience, and ethics. A conceptual framework about the six Es of landscape sustainability for designed landscapes is introduced. The interrelatedness,
opportunities, contradictions, and limitations of the conceptual framework are discussed in relation to human health/security,
ecosystem services, biodiversity, and resource management. The conceptual framework about the six Es of landscape sustainability
for designed landscapes follows the tradition in which landscape researchers and practitioners synthesize emerging trends
into conceptual frameworks for advancing basic and applied activities. 相似文献
996.
The present study investigated the effects of lipid oxidation on quality deterioration in the ordinary and dark muscles of skipjack tuna Katuwonus pelamis during the early stages of ice storage for 72 h. The lipid hydroperoxide content of the dark muscle was significantly higher (P < 0.01) than that of the ordinary muscle throughout 72 h of ice storage. The metmyoglobin content of the ordinary muscle gradually increased, and was accompanied with darkening in the fish meat color. On the other hand, the addition of sodium ascorbate or 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox®) to the ordinary muscle of skipjack tuna significantly inhibited the formation of lipid hydroperoxide as well as metmyoglobin formation. Thus, a decrease in α-tocopherol content in the ordinary muscle with antioxidant addition was not observed during ice storage period. In conclusion, the rate of lipid oxidation of skipjack tuna ordinary muscle is closely related to metmyoglobin formation, and the addition of antioxidants to fish meat is effective at inhibiting lipid oxidation as well as myoglobin oxidation in post-mortem meat. 相似文献
997.
Accumulation of organotin compounds and marine birnavirus detection in Korean ascidians 总被引:1,自引:0,他引:1
Kaoru Azumi Shinji Nakamura Shin-Ichi Kitamura Sung-Ju Jung Keisuke Kanehira Hisato Iwata Shinsuke Tanabe Satoru Suzuki 《Fisheries Science》2007,73(2):263-269
ABSTRACT: Recently, a serious disease spread extensively in aquaculture sites of the ascidian Halocynthia roretzi in Korea. To understand circumstances of ascidians in Korean aquaculture sites, residue levels of organotin compounds were analyzed, and detection of a marine birnavirus (MABV) in tissues of H. roretzi was attempted. Korean H. roretzi showed high concentrations of butyltins (mono, di, and tributyltins), especially in the gill, hepatopancreas, and digestive tract. However, there was no significant difference in the residues of butyltins in the hepatopancreas between diseased and non-diseased ascidians. The positive rate of MABV detection was high in the hepatopancreas, but also no significant difference was observed between diseased and non-diseased individuals. These observations suggest that an accumulation of tributyltin and a latency of MABV in H. roretzi tissues does not directly relate to the occurrence of the disease. 相似文献
998.
Pedro Domingues Ana Ferreira Lorenzo Marquez José P. Andrade Nelda López Carlos Rosas 《Aquaculture International》2008,16(3):215-229
The effects of feeding three natural frozen diets, grass shrimp (Palaemonetes sp.), crayfish (Procambarus clarkii) and fish (Sardina pilchardus) and two semi-humid artificial diets (based on fish powder) to mature cuttlefish, Sepia officinalis, were analysed. Growth and feeding rates (GR and FR, % BW day−1), food conversions (FC, %), and total protein and lipid composition of the diets were determined. Digestive gland to body
weight ratio and absorption efficiency were calculated for each diet. Cuttlefish fed shrimp and crayfish grew larger (1.5
and 1.1% BW day−1, respectively) compared to the other diets. Shrimp promoted the highest FC, followed by crayfish, and sardine. The highest
FR was obtained for cuttlefish fed crayfish (8.4% BW day−1). Although both artificial diets were accepted, none produced growth. A positive correlation (r = 0.96) between cuttlefish ingestion rate and digestive gland weight was obtained. Some cannibalism occurred among cuttlefish
fed the artificial diets during the last week of the experiment. According to the results obtained, P. clarkii could be used as an alternative prey to shrimp for rearing adult mature (>50 g) S. officinalis. 相似文献
999.
Dorafshan S Kalbassi MR Pourkazemi M Amiri BM Karimi SS 《Fish physiology and biochemistry》2008,34(3):195-200
The aim of this study was a comparison of key haematological features of diploid (2n) and triploid (3n) Caspian salmon (Salmo trutta caspius). Morphometric indices of erythrocytes were determined on blood smears by light microscopy. Triploidy significantly (P < 0.001) increased all morphometric indices measured in the erythrocytes including cell size, cell surface area, and cell
volume. The increase in cell size was larger for the major (27%) axis than for the minor (22%) axis, thus making erythrocytes
of 3n Caspian salmon more ellipsoidal. The estimated increase in erythrocyte nuclear volume (87%) was bigger than the theoretical
expected 50% increase. Haematological indices were measured manually by hemocytometry. Triploids had lower numbers of red
blood cells (RBC: 1,120,000 cells/mL in 2n vs. 700,000 cells/mL in 3n; P < 0.001) but they were larger in size (mean erythrocytic volume [MEV]: 363.1 nm3 in 2n vs. 483.3 nm3 in 3n; P < 0.001). The decrease in RBC number was not compensated by the increase in MEV and, thus, triploidy affected the haematocrit
(Hct: 38.8% in 2n vs. 33.06% in 3n; P < 0.05). Total blood hemoglobin concentration was lower in triploid fish (Hb: 9.9 g/dL in 2n vs. 8.9 g/dL in 3n; P < 0.05). In contrast, mean erythrocytic hemoglobin (MEH: 95 μg in 2n vs. 133.2 μg in 3n; P < 0.001) was higher for 3n Caspian salmon as a result of their larger erythrocytes, although MEH concentration (MEHC: 0.26 g/dL
in 2n vs. 0.27 g/dL in 3n) did not significantly differ (P > 0.05). White blood cell (WBC) counts (lymphocytes and neutrophiles) were measured and WBC/RBC ratios were calculated. There
were no significant differences in WBC (15,710 cells/mL in 2n vs. 12,683 cells/mL in 3n; P > 0.05), lymphocytes, and neutrophils as %WBC as well as WBC/RBC ratios between two ploidy levels (P > 0.05). Triploid Caspian salmon showed higher erythrocyte abnormalities such as ‘twisted’, ‘tailed’, and ‘anucleated’ cells
as well as high portions of immature RBC in blood smears in comparison with diploids (P < 0.001). 相似文献
1000.
Rui A. Gonçalves Tu Do Cam Nguyen Nhu Tri Gonçalo A. Santos Pedro Encarnação Le Thanh Hung 《Aquaculture International》2018,26(3):921-936
Triplicate groups of one hundred Tra catfish (8 g?±?0.2) were fed seven test diets containing increasing levels of AFB1 (0, 50, 100, 250, 500, and 1000 μg AFB1 kg?1). Additionally Mycofix® Secure was added at 1.5% to one diet containing 500 μg AFB1 kg?1. Results showed that Tra catfish are sensitive to AFB1. Reduction in weight gain (P?<?0.05) was observed for fish fed 50 μg AFB1 kg?1 and declined further with increasing levels of AFB1 in the diets. Fish fed diets contaminated with 500 and 1000 μg AFB1 kg?1 showed increased (P?>?0.05) hepatosomatic index (HIS), while an increase in adipose somatic index (ASI) was observed in fish fed 50 μg AFB1 kg?1 and above when compared to the control and Mycofix® diets. After 12 weeks, blood serum analysis revealed higher alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in fish fed the 50, 100, and 250 μg AFB1 kg?1 suggesting occurrence of liver damage. Disease resistance of fish exposed to Edwardsiella ictaluri was also compromised by the presence of AFB1 in the feed and was directly related to the contamination level. Seven days after Edwardsiella ictaluri exposure, survival rates were 50, 41.7, 31.7, and 8.3% for fish fed control, 50, 100, and 250 μg AFB1 kg?1, respectively. This trial shows that AFB1 at a level of 50 μg AFB1 kg?1 and above can affect fish performance and disease resistance. Application of an effective mycotoxin management in the feed seems to be useful to prevent the negative effects of AFB1. 相似文献