Effect of post-planting applications of granulated atrazine and fertiliser on the early growth of Eucalyptus nitens

A factorial experiment was established in a plantation of Eucalyptus nitens in southern Tasmania on a site which had formerly carried native eucalypt forest. Before planting, the site had been cleared of weeds with foliar translocated and pre-emergence herbicides. The first factor of the experiment examined the effects on tree growth of no post-planting weed control, control by hand or with three levels of application of granulated atrazine, 0.9, 1.8 and 8 kg ha^–1. The second factor examined the effects of no fertiliser application or the application of nitrogen as urea at 200 kg ha^–1 N plus triple superphosphate at 120 kg ha^–1 P. Tree growth responses to treatments were examined at 11, 16, 23 and 29 months of age.Fertiliser application increased tree height and diameter growth. Application of atrazine generally reduced height and diameter growth, but this was statistically significant only at the highest rate of application. Growth reduction occurred even though atrazine reduced post-planting weed cover on the site and hence potential competition with the tree crop for site resources. Hand weeding reduced the weed crop on the site even more than atrazine, but this did not increase growth of the tree crop.Given satisfactory establishment practices which include the use of herbicides pre-planting, it was concluded that post-planting weed regeneration was not sufficiently vigorous to warrant post-planting weed control on these sites. The use of atrazine for post-planting weed control could even be deleterious to eucalypts.     

Allelic Size Standards and Reference Genotypes to Unify International Cocoa (Theobroma cacao L.) Microsatellite Data

Standardisation of microsatellite allele profiles between laboratories is of fundamental importance to the transferability of genetic fingerprint data and the identification of clonal individuals held at multiple sites. Here we describe two methods of standardisation applied to the microsatellite fingerprinting of 429 Theobroma cacao L. trees representing 345 accessions held in the worlds largest Cocoa Intermediate Quarantine facility: the use of a partial allelic ladder through the production of 46 cloned and sequenced allelic standards (AJ748464 to AJ48509), and the use of standard genotypes selected to display a diverse allelic range. Until now a lack of accurate and transferable identification information has impeded efforts to genetically improve the cocoa crop. To address this need, a global initiative to fingerprint all international cocoa germplasm collections using a common set of 15 microsatellite markers is in progress. Data reported here have been deposited with the International Cocoa Germplasm Database and form the basis of a searchable resource for clonal identification. To our knowledge, this is the first quarantine facility to be completely genotyped using microsatellite markers for the purpose of quality control and clonal identification. Implications of the results for retrospective tracking of labelling errors are briefly explored.     

Energy investment in leaves of red maple and co-occurring oaks within a forested watershed

Despite its recent expansion in eastern US forests, red maple (Acer rubrum L.) generally exhibits a low leaf photosynthetic rate, leaf mass per unit area (LMA) and leaf nitrogen concentration ([N]) relative to co-occurring oaks (Quercus spp.). To evaluate these differences from the perspective of leaf energy investment, we compared leaf construction cost (CC) and leaf maintenance cost (MC) with leaf photosynthetic rate at saturating photon flux density and ambient CO2 partial pressure (Amax) in red maple and co-occurring red oak (Quercus rubra L.) and chestnut oak (Quercus prinus L.). We also examined relationships among leaf physiological, biochemical and structural characteristics of upper-canopy leaves of these three species at lower (wetter) and upper (drier) elevation sites of a watershed in the Black Rock Forest, Cornwall, NY, USA. Although A(max), leaf [N], leaf carbon concentration ([C]) and LMA were significantly less in red maple than in either oak species at both sites, CC per unit leaf area of red maple was 28.2 and 35.4% less than that of red oak at the lower and upper site, respectively, and 38.8 and 32% less than that of chestnut oak at the lower and upper site, respectively. Leaf MC per unit leaf area, which was positively associated with leaf CC (r2 = 0.95), was also significantly lower in red maple than in either oak species at both sites. When expressed per unit leaf area, A(max) was positively correlated with both CC (r2 = 0.65) and MC (r2 = 0.59). The cost/benefit ratio of CC/Amax of red maple was significantly less than that of chestnut oak at the lower site, however, CC/A(max) did not exhibit any significant interspecific differences at the upper site. Expressed per unit leaf area, CC was correlated positively with LMA (r2 = 0.90), leaf [N] (r2 = 0.97), and leaf [C] (r2 = 0.89), and negatively correlated with leaf molar carbon to nitrogen ratio (r2 = 0.92). Combined with red maple's general success in many oak-dominated forests, our findings suggest that reduced leaf-level photosynthetic capacity and related leaf characteristics in red maple are partially balanced by lower energy and resource requirements for leaf biomass construction and maintenance, which could enhance the competitive success of this species.     

Inhibition of response of Heliothis virescens to its natural pheromone by antipheromones

Four derivatives, (Z)-9-tetradecenyl trifluoroacetate and the corresponding trichloroacetate, N-methyl-(Z)-9-tetradecenyl carbamate and (Z)-12-heptadecen-2-one, have been shown to inhibit the response of Heliothis virescens to its natural pheromone. This inhibition was demonstrated to be reversible and has been suggested to be based on an associative interaction between the receptor site and the carbonyl group of the antipheromone.     

Comparative imaging of European eels (Anguilla anguilla) for the evaluation of swimbladder nematode (Anguillicoloides crassus) infestation

This study compares diagnostic imaging tools in detecting the parasitic swimbladder nematode Anguillicoloides crassus in Anguilla anguilla (L.) and focuses on ultrasound in an attempt to develop a non‐destructive, field diagnostic test. Ultrasound use could allow the parasite to be diagnosed without decreasing the number of critically endangered European eels through post‐mortem. In the preliminary study, eels were examined with computed radiography, computed tomography, magnetic resonance imaging, 14 MHz high‐end ultrasound and 5 MHz low‐end portable ultrasound, and the results were compared with post‐mortem findings. This ultrasound scanning technique did not produce any promising results. A second batch of eels was examined using the same high‐end and low‐end ultrasounds, but employing a different scanning technique and comparing the results with post‐mortem. This second study, scanning along the midline from below, allowed for the detection of anomalies associated with moderately infected animals. None of the eels used in this study were severely infected; thus, no conclusions can be made regarding the use of ultrasound in those animals. Overall, it was found that none of the techniques were useful in diagnosing mildly infected individuals; therefore, no single diagnostic imaging tool is sensitive enough to replace post‐mortem for definite diagnosis.     

Moving beyond landscape resistance: considerations for the future of connectivity modelling and conservation science

Landscape Ecology - Landscape connectivity, the extent to which a landscape facilitates the flow of ecological processes such as organism movement, has emerged as a central focus of landscape...     

Evaporatranspiration and growth of two contrasting species of eucalypts under non-limiting and limiting water availability

Changes in root zone water storage between 0 and 1 m depth were monitored at 14-day intervals under contrasting canopies ofEucalyptus nitens (a gum) andEucalyptus delegatensis (an ash) during their fourth and fifth years of growth and periods of non-limiting and limiting water availability, respectively. The leaf area index of the gum remained approximately double that of the ash throughout this time. Stem volume growth differed by factors of 1.8 and 2.0 betweenE. nitens andE. delegatensis while total water use differed by factors of 1.1 and 0.9 in two periods, respectively. A cumulative plot of volume growth as a function of total evapotranspiration suggested that stem volume growth ofE. nitens was less sensitive to severe water stress than that ofE. delegatensis.