正交设计法研究花锚药效成份的优化提取工艺

运用正交设计法研究了椭叶花锚中总黄酮的提取工艺,通过实验确定出了最佳的提取工艺条件。     

杨树修复重金属污染土壤的研究进展

总结杨树修复重金属污染土壤的优势、富集能力强杨树种/品种/无性系的筛选方法、重金属在杨树体内的富集和分配特性、重金属对杨树的影响及提高杨树对重金属污染土壤修复能力的措施,提出今后该领域研究重点。     

干热河谷几种典型造林树种的水分利用效率研究

干热河谷地区造林成活率低一直是困扰国内学者的难题之一,其中影响其成活的关键因子之一就是土壤水分。进行科学合理的节水灌溉就显得十分必要。相思适宜的土壤含水率（SWC）范围为12％～24％;合欢适宜的土壤含水率（SWC）范围为12％～28％;车桑子适宜的土壤含水率（SWC）范围为10％～26％。在此范围内,水分利用效率较高,光合速率水平也不低,林木能进行正常的生长及结实,不影响产量。植物的抗旱性与水分利用效率没有相关关系。植物的抗旱性强弱与其本身的形态、抗性生理生态特性有关。就本文中所涉及的几个指标来说,得出植物的抗旱性强弱顺序为：新银合欢（Leucaena sp．）〈车桑子（Dodonaea viscose）〈赤桉（Eucalyptus camaldulensis）〈台湾相思（Acacia confusa）;而水分利用效率的顺序则是：相思〈车桑子=桉树〈合欢。     

基于智能手机平台的猪饲料配方系统设计与实现

设计依猪只个体日龄、体重等参数而异的饲料配方是实现猪精细饲养的关键,目前已有很多具备此功能的饲料配方软件,但它们大多数基于PC平台。结合智能手机便于携带、可移动性好等优点开发的基于智能手机平台的猪饲料配方软件,便于携带且操作简单,使用NRC1998模型精确预测了猪营养需要量,配方结果更准确科学,饲料原料营养成分数据用XML文件贮存,数据读取方便,有效地降低了系统开发成本。     

Low‐carbon evaluation of rural neighborhood: A case study of Yanhe Village,Hubei Province,China

Climate change has become a global issue influencing human survival and development while low‐carbon is the inevitable choice. As the world’s largest emitter of greenhouse gases, China has become an important force that influences the cooperation of climate change. China is a large agricultural country, and the rural carbon emissions have been gradually increasing. We consider rural neighborhood as an important space for the use of low‐carbon ideas to address climate change. There is no specific assessment system for a rural neighborhood. The studies of rural neighborhood low‐carbon mainly focus on the method of calculation carbon emissions. However, only quantifying rural carbon emissions is insufficient. In this paper, a low‐carbon evaluation indicator system has been proposed for rural neighborhood and Fuzzy Comprehensive Evaluation has been applied to get the low‐carbon degree. Considering the influence and feedback inside the indicator system, Analytic Network Process was applied to get the weights. Furthermore, a case study was carried out for the using of the proposed method in an eco‐village of China. Our practice has proved that the system is easy to operate in rural neighborhood.     

红肉猕猴桃果实发育期不同果肉部位比较转录组分析

红肉猕猴桃中果皮和内果皮在果实发育不同时期色泽变化模式有显著差异,但目前对于其内在的分子调控机制尚不清楚。本研究中,利用高通量测序技术,对红肉猕猴桃(‘红实2号’)中果皮与内果皮4个不同发育时期(开花后0 d, 28 d, 75 d, 157 d,分别标注为T1时期, T2时期, T3时期, T4时期)的样本进行转录组测序。经去除低质量数据后,得到Clean reads数目为2 192～4 331万,检测到的基因数目为24 607～29 170个。在(log2 Ratio)≥1和FDR<0.05阈值下,中果皮和内果皮在T2、T3、T4时期检测到的差异基因数目分别为1 880个、4 799个、7 346个和4 31个、1 493个、7 700个。KEGG分析显示这些差异表达基因被富集到68个代谢途径,包括DNA复制、RNA转运、蛋白质输出、植物激素信号转导、类黄酮生物合成、花青素合成、淀粉与蔗糖的代谢等。此外我们还鉴定到13个花青素合成相关基因差异表达,大部分基因的表达量随果实的发育呈现下降趋势,这一结果与花青素含量变化模式相吻合。本研究不仅深化了对猕猴桃果实成色差异机理的认识,还对今后的彩色猕猴桃选育种工作有一定的帮助。     

紫色丝膜菌菌种分离与栽培研究

本文对贺兰山野生紫色丝膜菌进行了组织分离和人工驯化栽培的初步研究 ,结果表明 :该菌菌丝易于培养 ,子实体难于分化形成     

荣成俚岛人工鱼礁建设项目Ⅱ．人工鱼礁养护生物资源效果的评价

于2006年6月和2007年6月在荣成俚岛开展人工鱼礁投放前海域本底调查和投放后效果调查,分析比较了鱼礁区游泳动物、大型底栖动物的种类组成、生物多样性、种类丰度、CPUE和海藻等在人工鱼礁投放前后的变化。调查结果表明：人工鱼礁投放后,游泳动物和大型底栖动物种类总数、种类多样性指数和丰度均有所增加,分别是投礁前的1．78倍和1．32倍、1．47倍和1．22倍、1．68倍和1．62倍。游泳动物CPUE比投礁前增加1．39倍左右,其中鱼类的CPUE增加最多,尤其是大泷六线鱼和许氏平鲇等岩礁性鱼类,分别是投礁前的2．18倍和5．67倍。大型底栖动物种类有所增加,但CPUE略有减少。礁体上附着生长了马尾藻和海带等藻类。综合分析认为,人工鱼礁对游泳动物和大型底栖动物聚集和养护效果显著,鱼礁的投放也有利于海藻的附着、生长和藻场的恢复。     

农药的对靶喷撒技术在综合防治技术体系中的意义

在病虫害综合防治技术体系中,农药的不良副作用是一种消极因素。本文论述了产生这种副作用的原因,指出问题在于常用的粗放施药方法药剂沉积效率太低面常规喷撒方法又缺乏严格的喷撒技术指标。提出应发展农药的对靶喷撒技术,提高药剂在有效靶区的沉积效率,以减少药剂向非目标靶区的飘移损失,使综合防治技术体系更趋完善。文章介绍了对靶喷撒技术的研究发展概况。     

Effect of Ultraviolet Disinfection of Hydroponic Solutions on Pythium Root Rot and Non-target Bacteria

Dispersal of root pathogens is a major concern in closed hydroponic cultures. To limit dispersal, ultraviolet (UV) disinfection technology has been used to remove pathogens but its effect on non-target bacterial populations is largely unknown. In this study, the circulating solution was disinfected with various UV doses (i.e., 19, 38, 59, 88mJcm^–2) before recirculation. At the beginning of the experiment, the hydroponic solution was inoculated with Pythium aphanidermatum at 6.7±1.5CFUmL^–1. Four months later the population density of P. aphanidermatum reached 1030CFUmL^–1 in the control and 1028, 970, 610, and 521CFUmL^–1 in the solutions treated by the above UV doses. In all UV-treated solutions, significant (P<0.05) reduction of both Pythium and bacterial populations was noted. For the former, its reduction did not result in decrease of Pythium root rot nor increase of tomato fruit yield. For the latter, its reduction showed a concomitant decrease of the populations in the rhizosphere. The bacterial populations in the rhizosphere were 6.77×10⁹CFUmg^–1 fresh roots at the beginning of the experiment and were 7.89×10⁸, 9.93×10⁷, 7.33×10⁷, and 3.51×10⁷CFUmg^–1 fresh roots at the end of the experiment in the control, UV38, UV59, and UV88 treatments, respectively. The bacterial density also decreased with time in the control (UV0) although at a low rate. The results suggest that the attempt to control Pythium root rot by UV irradiation of recirculating solutions to remove P. aphanidermatum also affects the non-target bacterial populations in the rhizosphere. The interaction between the target pathogen and non-target bacterial flora in UV-treated hydroponics needs further investigation.