全文获取类型
收费全文 | 302篇 |
免费 | 7篇 |
专业分类
林业 | 50篇 |
农学 | 6篇 |
28篇 | |
综合类 | 5篇 |
农作物 | 10篇 |
水产渔业 | 46篇 |
畜牧兽医 | 148篇 |
园艺 | 1篇 |
植物保护 | 15篇 |
出版年
2022年 | 9篇 |
2021年 | 14篇 |
2020年 | 6篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 8篇 |
2016年 | 7篇 |
2015年 | 14篇 |
2014年 | 9篇 |
2013年 | 20篇 |
2012年 | 21篇 |
2011年 | 25篇 |
2010年 | 7篇 |
2009年 | 7篇 |
2008年 | 29篇 |
2007年 | 31篇 |
2006年 | 14篇 |
2005年 | 25篇 |
2004年 | 17篇 |
2003年 | 11篇 |
2002年 | 8篇 |
2001年 | 9篇 |
2000年 | 4篇 |
1998年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1989年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有309条查询结果,搜索用时 15 毫秒
211.
Yoshikawa T Moriyama A Kodama R Sasaki Y Sunagawa T Okazaki T Urashima A Nishida Y Arima A Inoue A Negishi T Yoshikawa Y Ihara T Maeda H 《Journal of toxicologic pathology》2011,24(4):215-222
To evaluate morphologic alterations in the thyroid gland in the second generation in cynomolgus monkeys, pregnant dams were exposed to high doses of thiamazole. In Experiment A, dams received thiamazole intragastrically via a nasogastric catheter from gestation day (GD) 50 to GD 150 or on the day before delivery. Initially, the dose level was 20 mg/kg/day (10 mg/kg twice daily); however, the dose level was subsequently decreased to 5 mg/kg/day (2.5 mg/kg twice daily), since deteriorated general conditions were observed in two dams. Six out of seven neonates died on the day of birth. The cause of neonatal death was tracheal compression and suffocation from goiter. The transplacental exposure to thiamazole affected the fetal thyroid glands and induced goiter in all neonates. The surviving neonate was necropsied 767 days after discontinuation of thiamazole exposure and showed reversibility of the induced changes. In Experiment B, dams were intragastrically administered thiamazole at 5 mg/kg/day (2.5 mg/kg twice daily) for treatment periods from GDs 51 to 70, 71 to 90, 91 to 110, 111 to 130 and 131 to 150. All fetuses showed enlarged thyroid glands but were viable. Histopathologically, hypertrophy and/or hyperplastic appearance of the follicular epithelium of the thyroid gland was observed at the end of each treatment period. The most active appearance of the follicular epithelium, consisting of crowded pedunculated structure, was demonstrated at end of the treatment period from GD 131 to 150. This is the first report on the morphology of fetal and neonatal goiter in the cynomolgus monkey. 相似文献
212.
Moriyama A Fujishima J Furukawa T Yoshikawa T Kodama R Sasaki Y Nagaoka T Kamimura Y Maeda H Hirai T Yamaguchi R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1459-1464
To clarify the morphological characteristics of the cynomolgus monkey immune system, we analyzed quantitative data on their lymphoid organs. Spleens, major lymph nodes and Peyer's patches were sampled from cynomolgus monkeys, and the lymphoid follicle and germinal center areas and percentages of CD3- and CD20-positive areas were calculated. All the organs analyzed showed large interindividual variations in the sizes of lymphoid follicles and germinal centers. Lymphoid follicle in the spleen, submandibular lymph nodes and Peyer's patches showed no marked difference in size. Germinal center size in the mesenteric lymph nodes and Peyer's patches were significantly smaller than those in the spleen. Areas containing T cells were largest in the lymph nodes, while those containing B cells were largest in the spleen and Peyer's patches. The mean size of the splenic lymphoid follicle in cynomolgus monkeys is larger than that in rats and similar to that in humans. Based on the large individual variation and the characteristics of lymphoid organs, it is important to use cynomolgus monkeys in standard toxicity studies. Taking advantage of the characteristics of each species enables reliable evaluation of the immunologic system in standard toxicity studies. 相似文献
213.
Yamazaki S Motoi Y Nagai K Ishinazaka T Asano M Suzuki M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1611-1616
We describe a method for determining the sex of sika deer (Cervus nippon yesoensis) from feces collected in the field. Using a nested polymerase chain reaction (nested PCR), partial sequences of the sex determination region of the Y chromosome (SRY) gene and X zinc finger protein (ZFX) gene were amplified. In 19 individuals with sex information, the correct sex was successfully detected and sequences of target amplicons were completely matched between muscle and feces from the rectum. Among 75 fecal samples collected noninvasively in the field, 68-71 samples (90.7-94.7%) yielded successful sex determinations. Using this technique, feces collected in the field would enhance the utility of genetic analysis. As a result, instead of biomaterials, these samples can serve as new or alternative materials. Finally, it can be expected that this technique will contribute to reveal in advanced detail of the population dynamics and genetic diversity that needed to carry out effective population control and to reduce the extinction risk of sika deer. 相似文献
214.
Chu Thi Thanh HUONG Takako MURANO Yukiko UNO Tatsufumi USUI Tsuyoshi YAMAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1583-1587
Poultry red mite (PRM,
Dermanyssus gallinae) is a blood-sucking ectoparasite as well as a
possible vector of several avian pathogens. In this study, to define the role of PRM in
the prevalence of avian infectious agents, we used polymerase chain reaction (PCR) to
check for the presence of seven pathogens: Avipox virus (APV), Fowl Adenovirus (FAdV),
Marek’s disease virus (MDV), Erysipelothrix rhusiopathiae (ER),
Salmonella enterica (SE), Mycoplasma synoviae (MS) and
Mycoplasma gallisepticum (MG). A total of 159 PRM samples collected
between 2004 and 2012 from 142 chicken farms in 38 prefectures in Japan were examined. APV
DNA was detected in 22 samples (13.8%), 19 of which were wild-type APV. 16S ribosomal RNA
(16S rRNA) of MS was detected in 15 samples (9.4%), and the mgc2 gene of
MG was detected in 2 samples (1.3%). Eight of 15 MS 16S rRNA sequences differed from the
vaccine sequence, indicating they were wild-type strains, while both of the MG
mgc2 gene sequences detected were identical to the vaccine sequences.
Of these avian pathogen-positive mite samples, three were positive for both wild-types of
APV and MS. On the other hand, the DNAs of ER, SE, FAdV and MDV were not detected in any
samples. These findings indicated that PRM can harbor the wild-type pathogens and might
play a role as a vector in spreading these diseases in farms. 相似文献
215.
Response of enzyme activities and metabolic intermediate concentrations to epinephrine administration in hepatopancreas and muscle of carp 总被引:1,自引:0,他引:1
Tsuyoshi Sugita Sadao Shimeno Yasunori Ohkubo Hidetsuyo Hosokawa Toshiro Masumoto 《Fisheries Science》2001,67(2):281-286
SUMMARY: Common carp Cyprinus carpio were administered intraperitoneally with 10 μmol epinephrine per 100 g bodyweight, and enzyme activities and metabolic intermediate concentrations were determined in the hepatopancreas and muscle. Glycogen phosphorylase a (GPase a) activity together with cyclic AMP (cAMP) concentration was increased, and glycogen content was decreased in the hepatopancreas and muscle at 2 h after the administration. The epinephrine administration also increased hepatopancreatic glucose-6-phosphatase and fructose-1,6-biphosphatase activities as well as serum glucose, lactate, and free amino acid concentrations. Furthermore, its administration increased phosphofructokinase activity together with lactate, fructose-6-phosphate, adenosine-5'-monophosphate, and adenosine-5'-diphosphate concentrations and decreased citrate concentration in the muscle. Thereafter, almost all parameter concentrations in the hepatopancreas and muscle recovered to the pre-administered levels during 24 h after the administration. These results suggest that epinephrine administration enhanced glycogenolysis and gluconeogenesis in the hepatopancreas, and released glucose into the bloodstream to supply it to the muscle. The blood glucose together with muscle glycogen seems to be metabolized through enhanced glycolysis in the muscle. 相似文献
216.
Takeshi Yamamoto Nobuhiro Suzuki Hirofumi Furuita Tsuyoshi Sugita Natsumi Tanaka Takanobu Goto 《Fisheries Science》2007,73(1):123-131
A feeding experiment was conducted to evaluate the supplemental effect of bile salts to a defatted soybean meal-based non-fish
meal diet for rainbow trout Oncorhynchus mykiss. A fish meal-based diet (FM) and two non-fish meal diets with and without bovine bile salts (SC+B and SC, respectively) were
fed to fish (13 g initial weight) for 10 weeks. Fish fed diet SC showed inferior growth and feed efficiency, while bile salt
supplementation improved the parameters to the same levels as fish fed diet FM. Crude fat and starch digestibility of diet
SC-fed fish decreased after the 10-week feeding trial compared to the data obtained with fish that had no experience of the
diet. Total biliary bile salt content and intestinal maltase activity of fish fed diet SC were the lowest among treatments,
while these parameters were improved by bile salt supplementation. Morphological changes occurred in the distal intestine
and liver of the diet SC group, although the histological features of fish fed diet SC+B were similar to those of fish fed
diet FM. These results suggest that bile salt supplementation to a soybean meal-based diet improves the nutrient utilization
by normalizing digestive processes in rainbow trout. 相似文献
217.
Tsuyoshi Komai Choko Kawabata Hiroaki Tojo Shinobu Gocho Eiji Ichishima 《Fisheries Science》2007,73(2):404-411
ABSTRACT: An acidic serine carboxypeptidase (CPase Tpa) from the hepatopancreas of Japanese common squid Todarodes pacificus was purified. Purified CPase Tpa had a molecular mass of 36 kDa on sodium dodecylsulfate–polyacrylamide gel electrophoresis, and an isoelectric point of 6.0. The optimum pH of CPase Tpa was pH 4.0. In investigating the specificity of CPase Tpa for several peptide substances, it was found that peptides with hydrophobic or bulky amino acid residues at the P1 position reacted well. The enzymatic activity was almost completely inhibited by p -chloromercuribenzoic acid, monoiodoacetic acid, diisopropylfluorophosphate and HgCl2 . This is the basis for its grouping in the serine carboxypeptidase family (EC 3. 4. 16. 5). The substrate specificity of CPase Tpa can be used to eliminate the bitterness of bitter peptides. In this study, the bitterness-reductive effect using bitter peptides prepared by hydrolyzing soy protein, casein and corn gluten with pepsin or trypsin was tested. The bitterness of soy peptide digested with pepsin was completely eliminated by treatment with CPase Tpa, whereas the bitterness of casein digested with trypsin and corn peptide digested with pepsin were somewhat less efficient. On the basis of these results, it is anticipated that CPase Tpa would be effective in eliminating the bitterness of some bitter peptides. 相似文献
218.
Xiping Cheng Kiyoshi Umeki Tsuyoshi Honjo Daijiro Mizusaki 《Journal of Forest Research》2014,19(1):86-96
We analyzed basal area (BA) growth using growth data obtained from permanent plots over 4 years for five major tree species in Anhui Province, eastern China. The studied species were dominant conifers (Pinus massoniana and Cunninghamia lanceolata) and co-dominant broad-leaved species (Castanopsis eyrei, Castanopsis sclerophylla, and Loropetalum chinense). A hierarchical Bayesian approach was used to estimate species-specific parameters and to quantify a spatially autocorrelated random effect. We selected a model that included only the following relevant predictor variables: initial size, asymmetric competition, spatially autocorrelated random effect, and random effect associated with plots. For all species analyzed, the model accounted for significant proportions of the variation (R 2 = 70–98 %) in BA growth rates. The initial slope of the relationship between BA growth rate and the initial BA tended to be steeper for P. massoniana than for C. lanceolata. The BA growth rate increased from an initial low value and then leveled off, with a lower maximum BA growth rate for C. lanceolata than for P. massoniana. The BA growth rate of P. massoniana was significantly affected by asymmetric competition with neighbors. The results of our analyses were used to predict to what extent thinning neighboring trees at different intensities would reduce competition impacts on BA growth of P. massoniana and C. lanceolata. Our results also helped to clarify the ecological characteristics of the species analyzed, as well as the spatial distribution of unknown factors influencing tree growth. 相似文献
219.
In order to prove a reduction function concerning flood damage according to land use, we have to compare evaluation indices,
such as flood concentration times. These can be derived from the calculation of the outflows from two basins where rainfall,
geology, geographical conditions, etc. are the same and only the land use is different. However, it is very difficult to obtain
comparable data from large basins under the above mentioned conditions. An elaborate observation for a long period is required
to obtain such data. In this paper, we aim to establish a calculation process that offers consideration factors from a viewpoint
that is different from a conventional research where elaborate observation is essential. Therefore, first we use easily obtainable
data of many basins, such as the information on the Internet, or data from official documents or the like, which are difficult
to deal with in hydrology. Then, we obtain several evaluation indices, which are coefficients close to the flood concentration
times in this paper, on each basin in which various kinds of land uses are mixed. We then perform the statistical analysis
with a land use ratio. In order to use various data on several basins, we evaluate the difference in the land use ratio in
the basin by using of a length of a riverside instead of an area in the basin. Moreover, since a river-mouth is at the end
of the riverside and a water stage observation point is not at the river-mouth, an error arises. Although there are several
problems in accuracy as mentioned above, the evaluation of the reduction function concerning the flood damage according to
land use will be feasible, automatically and efficiently, if the calculation process is established. 相似文献
220.
Noriaki Katsumata Tsuyoshi Yoshimura Kunio Tsunoda Yuji Imamura 《Journal of Wood Science》2007,53(4):320-323
Any means helpful for the promotion of termite feeding activity has potential for use in a matrix in termite bait application.
Therefore, energy transfer by gamma irradiation is worthy of consideration for converting wood into termite-accessible material.
Wood specimens gamma-irradiated at 100 kGy and at lower levels were tested for their degrees of polymerization (DP) of cellulose
and biological resistance. The DP of cellulose adversely decreased with increased doses of gamma irradiation. Termite wood
consumption rates, which were determined by laboratory tests using undifferentiated larvae (workers) of Coptotermes formosanus Shiraki, were significantly higher at 100 kGy than at other doses. On the other hand, the decay resistance of gamma-irradiated
wood against the fungi Fomitopsis palustris (Berkeley et Curtis) Murrill and Trametes versicolor (L. ex Fr.) Quel did not vary by irradiation dose. 相似文献