Prediction of cellular radiosensitivity from DNA damage induced by gamma-rays and carbon ion irradiation in canine tumor cells

Diseases of companion animals are shifting from infectious diseases to neoplasms (cancer), and since radiation therapy is one of the effective choices available for cancer treatment, the application of radiotherapy in veterinary medicine is likely to increase. However tumor tissues have different radiosensitivities, and therefore it is important to determine the intrinsic radiosensitivity of tumors in individual patients in advance of radiotherapy. We have studied the relationship between the surviving cell fraction measured by a clonogenic assay and DNA double strand breaks detected by a comet assay under neutral conditions in three canine tumor cell lines, after gamma-ray and carbon ion irradiation. In all the cell lines, cell death assessed by the clonogenic assay was much higher following irradiation with carbon ions than with gamma-rays. The initial and residual (4 hr) DNA damage due to gamma-ray and carbon ion irradiation were higher in a radiosensitive cell line than in a radioresistant cell line. The surviving cell fraction at 2 Gy (SF2) showed a tendency for correlation with both the initial and residual DNA damage. In particular, the residual damage per Gy was significantly correlated with SF2, regardless of the type of radiation. This indicates that cellular radiosensitivity can be predicted by detection of radiation-induced residual DNA damage.     

Phosphorus and potassium availability from cattle manure ash in relation to their extractability and grass tetany hazard

ABSTRACT

Due to a decrease in phosphorus (P) and potassium (K) mining, manure is incinerated to concentrate P and K in ash. To understand the alternative use of manure-derived ash as P and K sources, laboratory and greenhouse experiments were conducted to determine the relationship between extractability and P and K uptake in cattle manure ash (CMA) and that between CMA application and a grass tetany hazard. The results showed that more P was extracted with 2% citric acid (90% of the total P) than with 2% formic acid (72–84% of the total P). Ninety-one percent of the total K was soluble in water. A greenhouse pot experiment was conducted to test P and K availability to Guinea grass (Megathyrsus maximus). Cattle manure ash or calcium dihydrogen phosphate (CF) was incorporated into sandy soil at 10, 20, and 50 g P₂O₅ m^?2. Two combinations of CMA and CF were tested at 20 g P₂O₅ m^?2. Potassium rates followed K content in CMA applied at different rates of P equivalent to 19, 38, or 96 g K₂O m^?2. In four harvests, there was no significant difference in the total yields between CMA and CF treatments. The total P uptake was significantly lower in the CMA treatment than in the CF treatment, while it was not in the combined CMA and CF treatments. The P uptake in response to different extraction methods indicated that the extraction of P by 2% formic acid without sonication is recommended to predict P availability in CMA. The potassium uptake from CMA application was comparable to that from the KCl application, and excessive K occurred at 38 and 96 g K₂O m^?2. The grass tetany hazard ratio higher than 2.2 was observed at the beginning period at the lowest application rates of CMA and CF. In conclusion, the combination use of CMA and CF was better than the single use of CMA. Moreover, CMA would be an available K source, but the grass tetany hazard still needs to be considered in application rates and pretreatments.     

New biomarkers for increased intestinal permeability induced by dextran sodium sulphate and fasting in chickens

Increased intestinal permeability (IP) can lead to compromised health in chickens. As there is limited literature on in vivo biomarkers to assess increased IP in chickens, the objective of this study was to identify a reliable biomarker of IP using DSS ingestion and fasting models. Male Ross chickens (n = 48) were reared until day 14 on the floor pen in an animal care facility, randomized into the following groups: control, DSS and fasting (each with n = 16), and then placed in metabolism cages. DSS was administered in drinking water at 0.75% from days 16 to 21, while controls and fasted groups received water. All birds had free access to feed and water except the birds in the fasting group that were denied feed for 19.5 h on day 20. On day 21, all chickens were given two separate oral gavages comprising fluorescein isothiocyanate dextran (FITC‐d, 2.2 mg in 1 ml/bird) at time zero and lactulose, mannitol and rhamnose (LMR) sugars (0.25 g L, 0.05 g M and 0.05 g R in 2 ml/bird) at 60 min. Whole blood was collected from the brachial vein in a syringe 90 min post‐LMR sugar gavage. Serum FITC‐d and plasma LMR sugar concentrations were measured by spectrophotometry and high‐performance ion chromatography respectively. Plasma concentrations of intestinal fatty acid binding protein, diamine oxidase, tight junction protein (TJP), d ‐lactate and faecal α‐antitrypsin inhibitor concentration were also analysed by ELISA. FITC‐d increased significantly (p < 0.05) after fasting compared with control. L/M and L/R ratios for fasting and L/M ratio for DSS increased compared with control chickens (p < 0.05). TJP in plasma was significantly increased due to fasting but not DSS treatment, compared with controls. Other tests did not indicate changes in IP (p > 0.05). We concluded that FITC‐d and LMR sugar tests can be used in chickens to assess changes in IP.     

Nonselective computed tomography angiography for detecting arterial blood flow to the distal limb following trauma in two small equids

Two equids weighing <250 kg were examined several days after suffering severe mid‐diaphyseal dorsal metatarsal lacerations. Distal limb vascular disruption was suspected in both cases. Nonselective computed tomography angiography with contrast medium injected peripherally via the jugular veins was used to evaluate the vascular supply to the distal limb. The use of this imaging technique demonstrated either intact or disrupted distal limb vasculature. The imaging results were verified with a positive long‐term outcome in the case with a diagnosis of an intact vasculature and with histological findings of avascular necrosis in the case with a diagnosis of disrupted vasculature.     

Antibacterial activity of Nepalese wild mushrooms against <Emphasis Type="Italic">Staphylococcus aureus</Emphasis> and <Emphasis Type="Italic">Propionibacterium acnes</Emphasis>

The present study aims to reveal the antibacterial potential of the wild mushrooms of Nepal. Despite the recognition of the medicinal potential of the natural resources in this country, a systematic study on the bioactivities of the wild mushrooms is still lacking. Therefore, in an attempt to fill this gap, ethanol extracts of 90 Nepalese wild mushroom samples were tested for antibacterial activity against Staphylococcus aureus and Propionibacterium acnes. Staphylococcus aureus was comparatively more susceptible with Inonotus clemensiae exhibiting the least minimum bactericidal concentration (MBC) of 100 μg/mL. The major compound in I. clemensiae was identified to be hispidin using high resolution liquid chromatography–electron spray ionization mass spectrometry (LC–ESI-MS) and nuclear magnetic resonance (NMR) analyses. Furthermore, the minimum inhibitory concentration (MIC) and MBC values of hispidin were determined to be 25 and 100 µg/mL for S. aureus and P. acnes, respectively. These findings show that the Nepalese wild mushrooms have the potential to be a novel addition to the functional ingredients industry due to their strong antibacterial potential.     

Determination of anti‐oxidant activity by electron spin resonance spectroscopy

A method to assess anti‐oxidant activity quantitatively is presented. In this method, free radicals (OH·) were produced from the Fenton reaction. Electron spin resonance spectroscopy (ESR) was then used to determine the effectiveness of five anti‐oxidants to scavenge the free radicals. Anti‐oxidant activity was assessed as the percentage reduction of the ESR signal intensity relative to that of a control after 10 min. The order of the potency of the antioxidants at 4.8 mM was: caffeic acid > o‐coumarin > 6,7‐dihydroxy‐4‐methylcoumarin > catechin > scopoletin. In addition, pro‐oxidant activity (a higher ESR signal intensity than that of the control) was observed for catechin and scopoletin at low concentrations (below 3.6 mM ). © 2000 Society of Chemical Industry     

Comparative analysis of local and improved practices used by farmer seed production schools in Vietnam

Farmer seed production schools in combination with participatory field experimentation might be effective instruments to enhance the skills of farmers to produce high-quality seed in the informal seed system in Vietnam. Four hundred twenty nine, unreplicated on-farm experiments were carried out in four different provinces and analyzed by the farmer seed production schools. These experiments consisted of two adjacent seed production plots: one with common local practice and one with improved practice. Differences between the two plots in yield and profit were assessed. Averaged across provinces, yields of the “improved practice plots” were 0.43 Mg ha⁻¹ higher in the wet season and 0.78 Mg ha⁻¹ higher in the dry season than the “local practice plots”. In the Nam Dinh and Nghe An provinces more hills per m², more input of potassium and muck, and fewer seedlings per hill at transplanting contributed to the higher yields in the “improved practice plots”. In the provinces Binh Dinh and Dong Thap, using a drum seeder for sowing, reduced seed rate, less input of nitrogen fertilizer and more potassium contributed to the yield advantage of the “improved practice plots”. The profit of the improved practice plots was 212 US$ ha⁻¹ higher than the profit in the local practice plots. Rice growers can benefit from lower seed rates, better yields and higher prices when grains are sold as seeds. These findings may direct the seed policies and extension approaches in Vietnam.     

Effects of species and shelterbelt structure on wind speed reduction in shelter

Live shelterbelts are common elements in coastal land areas and play an important role in reducing wind speed and sand drift. A simple measured index, that well represents relationship between shelterbelt structure and wind speed reduction, is required by landowners to enable them in establishing more effective shelterbelts. A three-dimensional crown (3D) density is proposed, which can be easily identified through shelterbelt parameters including maximum height, shelterbelt width, vertical crown/stem area ratio, and horizontal crown/stem area ratio. The utility of the index was tested in 10-year-old Casuarina equisetifolia and in 7-year-old Acacia auriculiformis shelterbelts in north central Coast of Vietnam. There was a significant negative linear relationship (R ² = 0.64, p < 0.001) between 3D density and wind speed reduction efficiency, while there was no relationship between a two-dimensional crown density and wind speed reduction efficiency. Reduction efficiency was found to increase at higher wind speeds in shelterbelts of A. auriculiformis, but not C. equisetifolia. The A. auriculiformis shelterbelt was more efficient in reducing wind speed compared to C. equisetifolia shelterbelt. The former recovered 70 % wind speed at 130 m (16.5H) leeward, while it recovered 70 % at 85 m (8H) leeward in C. equisetifolia shelterbelt.     

Social differentiation revisited: A study of rural changes and peasant strategies in Vietnam

This article analyses the processes of transformation and differentiation since the 1950s in a Vietnamese rural village, hereafter called Chieng Hoa. ¹ It examines how radical changes in political discourse and economic policies at the national level have affected the welfare and social relations of villagers and how the latter have in turn coped, resisted, as well as shaped such structural changes. Using concrete life stories of local people, the article identifies the winners and losers in this transformation process, the trajectories households or individuals have taken to arrive at their current positions, and the strategies that they are adopting for the future. It demonstrates that differentiation in Chieng Hoa implies changes in social relations, including but not limited to relations of production, and that even within this single locality, differentiation can take various forms and processes over time, whether specific to or cutting across changes in macro‐policies. The article also reveals that in the often perceived equal collectivisation, inequalities still existed and became causes of differentiation in the subsequent decollectivisation period. However, while conditions for a permanent differentiation were present, such process has failed to materialise in the current integration period. Differentiation has become more unpredictable as past winners can lose out due to unstable market conditions.