Efficacy of clorsulon for the treatment of experimentally induced infections of Fasciola hepatica in goats

A dose titration study was undertaken to determine the efficacy of clorsulon against the adult stage of Fasciola hepatica in goats. Thirty-nine goats were experimentally infected with metacercariae of F hepatica. At 14 weeks after infection, each goat was assigned randomly to 1 of 5 groups. Goats in groups 1 to 4 received a single oral administration of clorsulon at dosages of 3.5, 7, 11, and 15 mg/kg of body weight, respectively. The fifth group of goats (control group) was infected with F hepatica, but were not treated with clorsulon. Postmortem examination of goats at 3 weeks after treatment revealed mean reductions in numbers of flukes of 83, 98, 99, and 100% for groups 1 to 4, respectively. Mean percentage of reduction in eggs following treatment of groups was 82, 98, 100, and 100%, respectively. The clinical effects of clorsulon in 24 goats that were not infected with F hepatica were studied. Goats in groups 1 to 3 received a single oral administration of clorsulon at dosages of 7, 21, and 35 mg/kg, respectively, every other day for a total of 3 doses/goat. Group-4 goats (control group) received a vehicle placebo. Goats in group 3 were subject to postmortem examination at 14 days after dosing. Abnormal signs or lesions that could be attributed to clorsulon were not found in any goat.     

A simple irrigation scheduling approach for pecans

Pecans are a major crop in New Mexico's Lower Rio Grande Valley (LRGV). It is estimated that New Mexico is responsible for about 21% of the world's pecan production (Lillywhite et al., 2007). Currently, approximately 12,000 ha of pecan orchards at various stages of growth consume 45% of the area's irrigation water. Pecan evapotranspiration (ET) varies with age, canopy cover, soil type, crop density and method of water management. Intense competition for the LRGV's limited water supply has created a serious need for better water management through improved irrigation scheduling. Annual pecan ET ranges from as low as 500 mm to as high as 1400 mm. Diversity of the pecan crop coefficient (K_c) and ET makes the task of irrigation scheduling for this crop very complicated. Using remote sensing technology and field ET measurements, a simple relationship was developed to relate crop coefficient and ET to canopy cover. This relationship is then used in combination with climate data to calculate daily and weekly water requirements for each orchard. The difference between annual ET values estimated from canopy cover and values measured with an eddy covariance flux tower ranged from 2 to 5%. The average ratio of estimated monthly ET values over measured ET values was 1.03 with the standard error of the estimate ranging from 10 to 20 mm/month. This methodology provides a simple tool that farmers can use to schedule irrigation of pecan orchards. Even though the methodology was developed for irrigation scheduling in the LRGV, it can be used in other locations by transferring the reference crop coefficients using K_c-GDD relationships.     

The combined mode of action of fipronil and amitraz on the motility of Rhipicephalus sanguineus

The motility of adult Rhipicephalus sanguineus was evaluated subsequent to treatments of amitraz, fipronil and the combination of fipronil plus amitraz against a vehicle control in a Petri dish assay using the LemnaTec Scanalyzer Imaging System. The assay was run using a fixed dilution of amitraz (0.32μg/cm(2)); serial dilutions of fipronil (1.3, 0.33, 0.08, 0.02, or 0.005μg/cm(2)); and the same serial dilutions of fipronil in combination with the fixed dilution of amitraz. Measurement of motility was made of unstimulated ticks and then after stimulation at 1, 4, 18-22, and 24h post exposure (hpe) of the Petri dishes. For the unstimulated ticks, there was no difference in motility between the amitraz treatment group and the fipronil plus amitraz treatment group at the early time points. However, these two treatment groups had significantly higher motility than the solvent control and fipronil treatment groups. The unstimulated ticks in the amitraz treatment group had significantly higher motility than the fipronil plus amitraz treatment group at the later time points. Measurements after stimulation demonstrated there was no difference in motility between the amitraz treatment group and the fipronil plus amitraz treatment group at the early time points. By 18 hpe, the fipronil plus amitraz treatment group had significantly lower motility than all other treatment groups and at 21-22 and 24 hpe the other treatment groups did not differ from the control group. The action could be divided in two phases in the combination experiment: phase 1: an early increase in motility due to amitraz is identified in both amitraz alone or fipronil plus amitraz groups; phase 2: the combination of fipronil plus amitraz caused a significantly greater reduction in motility, suggesting mortality of the ticks, compared to fipronil or amitraz alone. These results demonstrate a synergism resulting from the combination of fipronil plus amitraz.     

Hard choices: Making trade-offs between biodiversity conservation and human well-being

Win–win solutions that both conserve biodiversity and promote human well-being are difficult to realize. Trade-offs and the hard choices they entail are the norm. Since 2008, the Advancing Conservation in a Social Context (ACSC) research initiative has been investigating the complex trade-offs that exist between human well-being and biodiversity conservation goals, and between conservation and other economic, political and social agendas across multiple scales. Resolving trade-offs is difficult because social problems – of which conservation is one – can be perceived and understood in a variety of disparate ways, influenced (in part at least) by how people are raised and educated, their life experiences, and the options they have faced. Pre-existing assumptions about the “right” approach to conservation often obscure important differences in both power and understanding, and can limit the success of policy and programmatic interventions. The new conservation debate challenges conservationists to be explicit about losses, costs, and hard choices so they can be openly discussed and honestly negotiated. Not to do so can lead to unrealized expectations, and ultimately to unresolved conflict. This paper explores the background and limitations of win–win approaches to conservation and human well-being, discusses the prospect of approaching conservation challenges in terms of trade-offs and hard choices, and presents a set of guiding principles that can serve to orient strategic analysis and communication regarding trade-offs.     

Soil translocation by weeding on steep-slope swidden fields in northern Vietnam

This study investigated soil translocation associated with weeding on steeply sloped swidden fields attended by ethnic Da Bac Tay farmers in Hoa Binh Province in northern Vietnam. Annual soil loss rates of 4–6 Mg ha⁻¹ year⁻¹ were found on 20 m plots located on two separate hillslopes. Median soil flux rates were equivalent to 2.6–3.9 kg m⁻¹ pass⁻¹ for experiments conducted on slopes ranging from 0.54 to 0.84 m m⁻¹. The primary soil translocation process, the mechanical movement of soil via contact with a small hoe (ngheo), contributed approximately 60% of the weeding-related soil flux. Ravel, which is the rolling, bouncing, and sliding of soil clods downslope, was a secondary translocation process that accounted for almost 40% of the soil flux. Soil flux was most appropriately described with an exponential function that could predict the occurrence of ravel on steeper slopes. The observed soil fluxes were much smaller than those determined during weeding and hoeing at other tropical and subtropical sites, primarily because the tillage depth was very shallow (<1 cm) and weed density was low at the time of experimentation. The erosion rates associated with weeding were an order of magnitude lower than reported water erosion rates; therefore, the contribution to landscape change was believed to be small. Combined water and tillage erosion estimates indicated a possible unsustainable increase in soil loss on some steep-slope fields within the last few decades that has resulted from shorter fallow periods, longer periods of cultivation before fallowing, and greater weed pressure. Additional work is needed to verify these latter interpretations.     

Overriding imatinib resistance with a novel ABL kinase inhibitor

Resistance to the ABL kinase inhibitor imatinib (STI571 or Gleevec) in chronic myeloid leukemia (CML) occurs through selection for tumor cells harboring BCR-ABL kinase domain point mutations that interfere with drug binding. Crystallographic studies predict that most imatinib-resistant mutants should remain sensitive to inhibitors that bind ABL with less stringent conformational requirements. BMS-354825 is an orally bioavailable ABL kinase inhibitor with two-log increased potency relative to imatinib that retains activity against 14 of 15 imatinib-resistant BCR-ABL mutants. BMS-354825 prolongs survival of mice with BCR-ABL-driven disease and inhibits proliferation of BCR-ABL-positive bone marrow progenitor cells from patients with imatinib-sensitive and imatinib-resistant CML. These data illustrate how molecular insight into kinase inhibitor resistance can guide the design of second-generation targeted therapies.     

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease

Whole-genome sequencing of the protozoan pathogen Trypanosoma cruzi revealed that the diploid genome contains a predicted 22,570 proteins encoded by genes, of which 12,570 represent allelic pairs. Over 50% of the genome consists of repeated sequences, such as retrotransposons and genes for large families of surface molecules, which include trans-sialidases, mucins, gp63s, and a large novel family (>1300 copies) of mucin-associated surface protein (MASP) genes. Analyses of the T. cruzi, T. brucei, and Leishmania major (Tritryp) genomes imply differences from other eukaryotes in DNA repair and initiation of replication and reflect their unusual mitochondrial DNA. Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention.     

Enrichment planting of native species for biodiversity conservation in a logged tree plantation in Vietnam

Decades of deforestation and over logging have created large expanses of degraded lands in many countries including Vietnam. Reforestation may offer one means of mitigating these processes of degradation while sustaining biodiversity conservation. However a lack of information regarding trees, in particular threatened tree species has been identified as an important limitation in being able to reforest for biodiversity conservation. In the current study, conducted in the Tan Phu “protection forest”, the investigation surrounds the feasibility of biodiversity restoration in a fast-growing plantation after logging. Twelve threatened and native species mainly belonging to the Dipterocarpaceae and Fabaceae families have been considered. Seedlings grown in a local nursery have been planted under the canopy of a 50 ha pioneer forest, with a distance of 5 m between plants and between rows. The experimental design consists of blocks, each one with a replication of a 16-tree plot for each species. Four years after plantation, the survival rate of seedlings is high (>70 %), except for Dipterocarpus costatus (<10 %). Growth shows possible inhibitions under a closed canopy but the mean annual increment is generally satisfactory. The early performance of the seedlings is promising to make this plantation a good example of biodiversity restoration. These results could enhance the reforestation efforts of forest managers and encourage them to use native tree species including threatened ones in order to restore a high level of biodiversity in tropical degraded forests.     

Synthesis and bioactivity of some new N-aryl/alkyl/cyclohexyl-N'-(2,3-dihydro-2-oxo-4H-benz[e][1,3,2]oxazaphosphorin-2-yl) ureas

Several new substituted oxazaphosphorinyl urea derivatives of the type RR'P(O)NHC(O)NHR' were synthesized from alpha-(3-chloro-4-fluoroanilino)-o-cresol by reaction with chlorides of aryl/alkyl/cyclohexyl carbamidophosphoric acids in the presence of triethylamine at 0-50 degrees C. Their significant insecticidal and antimicrobial activity and promotion of Rhizobium bacteria growth in the soil without effect on the host tissue suggests their possible commercial application as ecofriendly pesticides and antimicrobial agents.