Grain yield and its component traits are essential targets in maize breeding. These traits are genetically complex and controlled by a large number of quantitative trait loci (QTL). The aim of this study was to compile reported QTL and major genes for grain yield and its component traits in a QTL atlas, as a valuable resource for the maize community. To this end, 1,177 QTL related to maize yield were collected from 56 studies published between 1992 and 2018. These QTL were projected to genetic map “IBM2 2008 Neighbors”, which led to the identification of 135 meta-QTL. Some genomic regions appear to be hotspots for yield-related meta-QTL, often affecting more than one of the investigated traits. Moreover, we catalogued 20 major maize loci associated with yield and identified 65 maize homologs of 21 rice yield-related genes. Interestingly, we found that a significant proportion of them are located in meta-QTL regions. Collectively, this study provides a reference for QTL fine-mapping and gene cloning, as well as for molecular marker-assisted breeding of yield-related traits in maize. 相似文献
A prospective study was conducted to determine the sensitivity and specificity of diagnosis of portosystemic shunts (PSS) and the accuracy of anatomically locating single congenital PSS in dogs using magnetic resonance angiography (MRA). MRA was performed on 10 normal dogs and 23 dogs with PSS. Sensitivity and specificity of MRA to diagnose any shunt among all dogs were 80% and 100%, respectively. Among dogs identified with PSS, sensitivity and specificity of MRA for diagnosis of multiple extrahepatic shunts were 63% and 97%, respectively, and for diagnosis of single congenital shunts were 79% and 100%, respectively. Using MRA, radiologists correctly identified shunts as extrahepatic or intrahepatic in 83% of patients and correctly identified the origin and insertion of the shunts in 57% and 97% of patients, respectively. Use of MRA is specific for diagnosis of PSS and is a sensitive indicator of anatomic location of single congenital portosystemic shunts. 相似文献
Despite regular reforms, problems under the EU's Common Fisheries Policy (CFP) persist. In order to identify priorities for future reforms of the policy, we developed an analytical framework consisting of 17 criteria and specifying indicators, derived from scientific, wider fisheries, and common resources literature. We applied the framework to the CFP governance system, its regulations, institutions, and processes at EU as well as member state level. The results show that the CFP does not fully meet any of the 17 criteria for an effective resource policy. Its performance was assessed as “neutral” regarding 10 criteria and “negative” regarding seven criteria. Trend analysis shows that there is a slightly positive trend regarding the CFP's performance, with five criteria trending positively, 11 showing a neutral trend and only one criterion trending negatively (simplicity of rules). The analysis identified five criteria which are performing badly and have not improved over time: simplicity of rules, user‐pays principle, resource efficiency, accountability, and compliance mechanisms. Future reforms of the CFP should first and foremost address these criteria while continuing efforts to improve the CFP's performance regarding other criteria. The evaluation of the CFP demonstrates the applicability of the analytical framework which can also be applied to other multilevel fisheries governance systems. Moreover, the results can inform reforms of Regional Fisheries Management Organisations. Like the CFP, these institutions manage transboundary fisheries and have not effectively addressed the issues of resource rent capture and resource efficiency. 相似文献
Around 30% of European agricultural landscapes are classified as high nature value (HNV) farmlands. Current policies emphasize the multifunctionality of these landscapes, but little is known about the positive and negative associations of multiple ecosystem services within HNV farmland.
Objectives
This study aims to identify perceived ecosystem services synergies, trade-offs, and bundles in agricultural landscapes of HNV from a socio-cultural perspective.
Methods
We performed a participatory mapping survey of 10 ecosystem services categories among 2301 rural residents in 13 European sites. We analyzed bivariate synergies and trade-offs between perceived ecosystem services through nonparametric correlation analyses. Spatial bundles of perceived ecosystem services were identified through hierarchical cluster analysis. Multinomial logit models were used to assess the influence of land cover on generating associations of ecosystem services.
Results
We find two strong and 16 moderate synergies of perceived ecosystem services (out of 46 possible ecosystem services pairs), mainly among different cultural ecosystem services. We do not reveal moderate or strong trade-offs. We identify five spatial bundles of ecosystem services, termed “Ecosystem services coldspots”, “Wild harvesting ranges”, “Nature areas”, “Recreational spaces”, and “Ecosystem services hotspots”. Of all land-cover co-variates, natural areas, urban areas, and roads have the strongest explanatory power.
Conclusions
Our study complements prevailing biophysical and economic analyses of ecosystem services synergies, trade-offs and bundles by a spatially explicit, socio-cultural perspective. We conclude that socio-cultural mapping of ecosystem services is useful for understanding the perceived multifunctionality of a landscape.
Efficacy and mechanism of action of marine alkaloid 3,10-dibromofascaplysin (DBF) were investigated in human prostate cancer (PCa) cells harboring different levels of drug resistance. Anticancer activity was observed across all cell lines examined without signs of cross-resistance to androgen receptor targeting agents (ARTA) or taxane based chemotherapy. Kinome analysis followed by functional investigation identified JNK1/2 to be one of the molecular targets of DBF in 22Rv1 cells. In contrast, no activation of p38 and ERK1/2 MAPKs was observed. Inhibition of the drug-induced JNK1/2 activation or of the basal p38 activity resulted in increased cytotoxicity of DBF, whereas an active ERK1/2 was identified to be important for anticancer activity of the alkaloid. Synergistic effects of DBF were observed in combination with PARP-inhibitor olaparib most likely due to the induction of ROS production by the marine alkaloid. In addition, DBF intensified effects of platinum-based drugs cisplatin and carboplatin, and taxane derivatives docetaxel and cabazitaxel. Finally, DBF inhibited AR-signaling and resensitized AR-V7-positive 22Rv1 prostate cancer cells to enzalutamide, presumably due to AR-V7 down-regulation. These findings propose DBF to be a promising novel drug candidate for the treatment of human PCa regardless of resistance to standard therapy. 相似文献
Soybean[Glycine max(L.)Merr.]is a global protein source and is currently expanding in Central and Northern Europe.Protein and oil content are two important quality traits that have been studied in different germplasm,however,their genetic architecture in earlymaturing European soybean has not been investigated yet.In this study,we therefore performed QTL mapping for both traits using 944 recombinant inbred lines derived from eight families from a half-diallel crossing design.We identified five QTL for each trait,with the QTL on chromosomes 8,15,and 20 being identified for both protein content and oil content.The known major QTL on chromosome 20 was detected in four families whereas the other QTL were only found in single families.Further analyses revealed the QTL to have pleiotropic but inverse effects on both traits.The effect of the major QTL was comparable between families,illustrating that it is largely independent from the genetic background.Collectively,our results illustrate the quantitative nature of protein and oil content in early European soybean.Marker-assisted selection for the QTL is possible,but the inverse effect on protein and oil content should be kept in mind. 相似文献
Marine alkaloid fascaplysin and its derivatives are known to exhibit promising anticancer properties in vitro and in vivo. However, toxicity of these molecules to non-cancer cells was identified as a main limitation for their clinical use. Here, for the very first time, we synthesized a library of fascaplysin derivatives covering all possible substituent introduction sites, i.e., cycles A, C and E of the 12H-pyrido[1-2-a:3,4-b’]diindole system. Their selectivity towards human prostate cancer versus non-cancer cells, as well as the effects on cellular metabolism, membrane integrity, cell cycle progression, apoptosis induction and their ability to intercalate into DNA were investigated. A pronounced selectivity for cancer cells was observed for the family of di- and trisubstituted halogen derivatives (modification of cycles A and E), while a modification of cycle C resulted in a stronger activity in therapy-resistant PC-3 cells. Among others, 3,10-dibromofascaplysin exhibited the highest selectivity, presumably due to the cytostatic effects executed via the targeting of cellular metabolism. Moreover, an introduction of radical substituents at C-9, C-10 or C-10 plus C-3 resulted in a notable reduction in DNA intercalating activity and improved selectivity. Taken together, our research contributes to understanding the structure–activity relationships of fascaplysin alkaloids and defines further directions of the structural optimization. 相似文献
Agricultural management is a major factor driving the change of faunal richness in anthropogenic landscapes. Thus, there is
an urgent need to develop tools that allow decision-makers to understand better intended and unintended effects of agricultural
policy measures on biodiversity. Here we demonstrate the potential of such a tool by combining a socio-economic model with
the biodiversity model GEPARD to forecast the response of bird and carabid species richness to two scenarios of agricultural
subsidies: (1) subsidies based on production levels and prices and (2) direct income support that is independent of production
levels. We focussed on farmland of the Lahn-Dill area, Germany, as an example of European regions with low intensity farming.
GEPARD predicts faunal richness and is based on multi-scaled resource-selection functions. Under both scenarios the area of
predicted losses in species richness of birds and carabids was larger than the area of predicted gains in species richness.
However, the area with predicted losses of avian richness was smaller under the direct income support scenario than under
the production-based subsidy scenario, whereas the area with predicted losses of carabid species richness was smaller under
the production-based subsidy scenario than under the direct income support. Yet locally, richness gains of up to four species
were predicted for carabids under both scenarios. We conclude that the sometimes contrasting and heterogeneous responses of
birds and carabids at different localities suggest the need for spatially targeted subsidy schemes. With the help of the GIS-based
approach presented in this study, prediction maps on potential changes in local and regional species richness can be easily
generated. 相似文献