应用采食模式改进蛋鸡饲喂制度研究

用40周龄 ISA 蛋鸡2116只,随机分为试验组和对照组,同幢鸡舍的2492只为大群组,根据基础日粮,均日喂二次,大群组日喂基础日粮四次,以研究对蛋鸡的影响。结果表明,试验组与对照组的产蛋率、蛋重及蛋的品质均无显著差异,但试验组的饲料成本减少14.74％,主要营养素蛋白质、能量和氨基酸的消耗也分别降低2.17％、2.92％和12.88％。作者认为,蛋鸡对饲料中营养素的利用能力,可能存在“时间节律”性因素的影响。     

Γ（Gamma）分布及其在辽东天然阔叶混交林中的应用

本又从Γ分布的构造及性状入手，研究了用Γ分布拟合天然阔叶混交林直径分布结构，取得了拟合成功率为９５．２％的理想结果，较原林业中常用的Ｗｅｉｂｕｌｌ分布的拟合成功率提高了１９．０％。     

潍坊萝卜中芜菁花叶病毒分离物的分子特性及CP基因的表达

 从表现红心病的潍坊萝卜块根上得到一芜菁花叶病毒( Turnip m osaic virus, TuMV) 分离物(TuMV-Ra) , 通过RT-PCR获得了该分离物的外壳蛋白(CP) 基因, 对其进行了序列测定, 并将其核苷酸序列及推导出的氨基酸序列与GenBank中登录的其它20个TuMV萝卜分离物的相应序列进行比较和分析。结果表明, TuMV-Ra与这20个分离物CP基因核苷酸序列的同源性为89.9% ～99.0% , CP氨基酸序列同源性为94.8%～99.7%; 其中与中国浙江杭州萝卜的两个分离物HZLB1、HZLB2同源性最高, 核苷酸序列的同源性为98.7%和99.0% , 氨基酸序列同源性为99.0%和99.7%。TuMV-Ra与1999年从表现相同症状的潍坊萝卜得到的分离物CHINA-WF CP基因核苷酸同源性为93.66% , 氨基酸同源性为96.53% , 说明病毒发生了比较大的变异, 而且变异的位点主要在CP的N末端。将TuMV-Ra CP基因克隆到原核表达载体pET-22b ( + ) , 在大肠杆菌BL21 (DE3) 中表达出分子量为38 kD的融合蛋白。Western blotting分析证明TuMV-Ra CP在大肠杆菌中得到了正确表达。     

2011—2019年山东省部分规模猪场猪瘟免疫效果监测

为了解山东省规模猪场的猪瘟免疫效果及变化趋势,2011—2019年在16个地市717个规模猪场,采集血清样品22 465份,采用猪瘟抗体ELISA方法进行抗体检测,并比对不同年份和不同场点的免疫水平。结果显示:2011—2019年,全省16个地市种猪场的场群免疫合格率为73.84%,个体合格率为77.54%;商品代猪场的场群免疫合格率为63.96%,个体免疫合格率为71.79%。从不同年份看,规模猪场的猪瘟免疫抗体水平呈波浪状变化,2011—2012年免疫水平较高,2013—2014年较低,2015—2016年再次升高,2017—2019年免疫水平又出现下降;从不同场点看,无论是个体免疫效果,还是群体免疫效果,种猪场均优于商品代猪场。结果表明,山东省规模猪场的总体猪瘟免疫抗体水平不高且不稳定,因而规模猪场应持续加强猪瘟的免疫和监测,尤其是商品代猪群,以降低猪瘟的发生风险。     

Isolation and identification of oligomers from partial degradation of lime fruit cutin

Complementary degradative treatments with low-temperature hydrofluoric acid and methanolic potassium hydroxide have been used to investigate the protective biopolymer cutin from Citrus aurantifolia (lime) fruits, augmenting prior enzymatic and chemical strategies to yield a more comprehensive view of its molecular architecture. Analysis of the resulting soluble oligomeric fragments with one- and two-dimensional NMR and MS methods identified a new dimer and three trimeric esters of primary alcohols based on 10,16-dihydroxyhexadecanoic acid and 10-oxo-16-hydroxyhexadecanoic acid units. Whereas only 10-oxo-16-hydroxyhexadecanoic acid units were found in the oligomers from hydrofluoric acid treatments, the dimer and trimer products isolated to date using diverse degradative methods included six of the seven possible stoichiometric ratios of monomer units. A novel glucoside-linked hydroxyfatty acid tetramer was also identified provisionally, suggesting that the cutin biopolymer can be bound covalently to the plant cell wall. Although the current findings suggest that the predominant molecular architecture of this protective polymer in lime fruits involves esters of primary and secondary alcohols based on long-chain hydroxyfatty acids, the possibility of additional cross-linking to enhance structural integrity is underscored by these and related findings of nonstandard cutin molecular architectures.     

Development and characterization of a new marine fish cell line from turbot (<Emphasis Type="Italic">Scophthalmus maximus</Emphasis>)

A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with HEPES, antibiotics, fetal bovine serum (FBS), 2-Mercaptoethanol (2-Me), and basic fibroblast growth factor (bFGF). The suitable growth temperature for TK cells was 24°C, and microscopically, TK cells were composed of fibroblast-like cells. Chromosome analysis revealed that the TK cell line has a normal diploid karyotype with 2n = 44. Two fish viruses LCDV-C (lymphocystis disease virus from China) and TRBIV (turbot reddish body iridovirus) were used to determine the virus susceptibility of TK cell line. The TK cell line was found to be susceptible to TRBIV, and the infection was confirmed by cytopathic effect (CPE) and transmission electron microscopy, which detected the viral particles in the cytoplasm of virus-infected cells. Finally, significant green fluorescent signals were observed when the TK cells were transfected with pEGFP-N3 vector, indicating its potential utility for fish virus study and genetic manipulation.     

基于土壤饱和入渗理论计算盐碱地冲洗定额

盐渍土是地球上分布最广的一种土壤类型,约占陆地总面积的1/4。据统计全世界盐渍土有9·54×108hm2。中国盐渍土面积大部分分布在新疆、甘肃河西走廊、青海柴达木、宁夏银川平原和内蒙古西部等地[1]。根据1990~1993年原全国农业区划委员会调查中国农业综合开发盐碱型后备资源3·75×106hm2,占耕地总面积的3·1%[2]。盐碱的改良利用一直受到世界各国的普遍重视,冲洗改良是利用盐碱地的重要水利措施。冲洗定额是冲洗改良盐碱地的一项重要技术指标。如何正确合理地确定冲洗定额是冲洗改良盐碱地技术中的一个重要环节。目前计算冲洗定额的方法主要有列果斯塔也夫经验公式、沃洛布耶夫公式、阿塞拜疆水工设计     

文体学在英语专业低年级口语教学中的应用

从语音和词汇两个层面探讨了将文体学理论应用于英语专业低年级口语教学的必要性和可行性,提出培养学生的文体意识在提高学生口头交际能力上具有重要的作用。     

转录因子FaNAC56在草莓果实成熟中的功能分析

[目的]研究NAC转录因子FaNAC56在草莓果实成熟中的作用.[方法]根据前期果实成熟过程中的蛋白组数据,以八倍体红颜草莓为试材,克隆FaNAC56基因及其启动子.在蛋白水平,利用MEGA构建FaNAC56系统进化树,并通过生物信息学预测其编码蛋白的二级结构,以及利用烟草进行亚细胞定位;在转录调控水平,利用预测网站分...