Spatial modelling of the fraction of photosynthetically active radiation absorbed by a boreal mixedwood forest using a lidar–hyperspectral approach

The spatial variability of the fraction of photosynthetically active radiation absorbed by the canopy (fPAR) was characterized for a heterogeneous boreal mixedwood forest site located in northern Ontario, Canada, based on relationships found between fPAR and light detection and ranging (lidar) data over different canopy architectures. Estimates of fPAR were derived from radiation measurements made above the canopy at a flux tower and below-canopy radiation was measured across a range of species compositions and canopy architectures. Airborne lidar data were used to characterize spatial variability of canopy structure around the flux tower and a map of mean canopy chlorophyll concentration was derived from airborne hyperspectral imagery. Different volumes of lidar points for the locations directly above each photosynthetically active radiation (PAR) sensor were examined to determine if there is an optimal method of relating lidar returns to estimated fPAR values.The strongest correlations between mean lidar height and fPAR occurred when using points that fell within a theoretical cone which originated at the PAR sensor having a solid angle α = 55°. For diffuse conditions, the correlation (r) between mean lidar height versus fPAR × chlorophyll was stronger than between mean lidar height versus fPAR by 8% for mean daily fPAR and from 10 to 20% for diurnal fPAR, depending on solar zenith angle. For direct light conditions, the relationship was improved by 12% for mean daily fPAR and 12–41% for diurnal relationships.Linear regression models of mean daily fPAR × chlorophyll versus mean lidar height were used in conjunction with gridded lidar data and the canopy chlorophyll map to generate maps of mean daily fPAR for direct and diffuse sunlight conditions. Site average fPAR calculated from these maps was 0.79 for direct light conditions and 0.78 for diffuse conditions. When compared to point estimates of mean daily fPAR calculated on the tower, the average fPAR was significantly lower than the point estimate. Subtracting the direct sunlight fPAR map from the diffuse sunlight fPAR map revealed a distinct spatial pattern showing that areas with open canopies and relatively low chlorophyll (e.g., black spruce patches) have a higher fPAR under direct sunlight conditions, while closed canopies with higher chlorophyll (e.g., deciduous species) absorb more PAR under diffuse conditions. These findings have implications for scaling from point measurements at flux towers to larger resolution satellite imagery and addressing local scale heterogeneity in flux tower footprints.     

The Use of Biofilters to Reduce Atmospheric Methane Emissions from Landfills: Part I. Biofilter Design

Previous laboratory research has shown that biofilters have the potential to reduce CH₄ emissions from landfills by as much as 83%. However, to achieve this level of CH₄ reduction biofilters must be properly designed. The present study was conducted to develop a method for properly designing biofilters based on landfill size and location. A quadratic equation was developed to describe the dependence of CH₄ oxidation rate in a sandy loam textured soil as a function of soil temperature, soil moisture and ammonium nitrogen concentration. Using this equation and the average monthly soil temperature and moisture contents for the largest cities of each of the 48 contiguous states, the monthly CH₄ oxidation rate at each location was calculated. Then, assuming a standard landfill depth of 27.6 m, and a standard area of 121,500 m², the required biofilter size was calculated. Finally, the ratio of biofilter size to landfill size was calculated. Design calculations for biofilters located in the states of Alabama, Florida, Georgia, Louisiana, Mississippi, North Carolina, South Carolina and Texas where the CH₄ oxidation rates are relatively high throughout the year indicate that the necessary biofilter sizes are small. In addition, biofilters in these states may be expected to be effective throughout the year. In contrast, the calculations indicated biofilter systems in the states of Idaho, Minnesota and North Dakota will have much lower efficiencies during much of the year due to unfavorable soil moisture and temperature ranges. Given proper design, installation and management, a biofilter should be capable of achieving a significant reduction in atmospheric CH₄ emission as compared to emissions from the same landfill without a biofilter.     

Validating the use of Cs measurements to estimate rates of soil redistribution by wind

Wind erosion has degraded over one-half billion hectares of land worldwide. ¹³⁷Cesium (¹³⁷Cs) has been used as a tracer to study long-term rates of soil redistribution by water and, to a lesser extent, by wind. Early studies assumed that the decline in ¹³⁷Cs activity for a potentially eroded soil relative to that for an uneroded soil was linearly proportional to soil loss. More recently, models have emerged that consider the effects of soil cultivation and the particle surface area-dependent partitioning of ¹³⁷Cs on soils. We investigated the partitioning of ¹³⁷Cs in wind-eroded sediments and with soil surface samples sieved into contiguous ranges of particle sizes. We also compared the ¹³⁷Cs activities and stratification of several adjacent soils with known wind erosion and deposition histories. Finally, we tested ¹³⁷Cs-based soil loss models with measured data from sites with documented histories. ¹³⁷Cs activities and mean particle diameters of aeolian samples agreed well with the ¹³⁷Cs activities and respective mean diameters of the sieved surface soil samples. Good agreement between model estimations and measured data indicated that ¹³⁷Cs models developed to estimate soil redistribution by water were also applicable to soil redistribution by wind provided that the models contained an appropriate particle size correction parameter.     

Novel Alkaloids from Marine Actinobacteria: Discovery and Characterization

The marine environment is an excellent resource for natural products with therapeutic potential. Its microbial inhabitants, often associated with other marine organisms, are specialized in the synthesis of bioactive secondary metabolites. Similar to their terrestrial counterparts, marine Actinobacteria are a prevalent source of these natural products. Here, we discuss 77 newly discovered alkaloids produced by such marine Actinobacteria between 2017 and mid-2021, as well as the strategies employed in their elucidation. While 12 different classes of alkaloids were unraveled, indoles, diketopiperazines, glutarimides, indolizidines, and pyrroles were most dominant. Discoveries were mainly based on experimental approaches where microbial extracts were analyzed in relation to novel compounds. Although such experimental procedures have proven useful in the past, the methodologies need adaptations to limit the chance of compound rediscovery. On the other hand, genome mining provides a different angle for natural product discovery. While the technology is still relatively young compared to experimental screening, significant improvement has been made in recent years. Together with synthetic biology tools, both genome mining and extract screening provide excellent opportunities for continued drug discovery from marine Actinobacteria.     

Hydrology influences breeding time in the white-throated dipper

While nitrogen and carbon stable isotope values can reflect ecological segregation, prey choice and spatial distribution in seabirds, the interpretation of bulk stable isotope values is frequently hampered by a lack of isotopic baseline data. In this study, we used compound-specific isotope analyses of amino acids (CSIA-AA) to overcome this constraint and to study interspecific differences, seasonal and historical changes in trophic positions of five seabird species, three penguins and two petrels, from a sub-Antarctic seabird community. CSIA-AA allowed comparing trophic positions of seabirds with temperate and polar distributions. Gentoo and Magellanic penguins had the highest trophic positions during the breeding season (3.7 and 3.9), but decreased these (2.9 and 3.3) during the feed-up for moult. Intra-specific differences were also detected in Thin-billed prions, where carbon isotope values clearly separated individuals with polar and temperate distributions, both in the breeding and interbreeding periods. Thin-billed prions that foraged in polar waters had lower trophic positions (3.2) than conspecifics foraging in temperate waters (3.8). We further investigated historical changes by comparing museum samples with samples collected recently. Our pilot study suggests that Rockhopper penguins, Magellanic penguins and Thin-billed prions with temperate non-breeding distributions had retained their trophic levels over a 90–100 year period, while Gentoo penguins and Thin-billed prions with polar non-breeding distributions had decreased trophic levels compared to historical samples. In contrast, Wilson’s storm-petrels had slightly increased trophic levels compared to samples taken in 1924–1930. We applied compound-specific stable isotope analyses across a range of contexts, from intra-specific comparisons between stages of the breeding cycle to inter-specific seabird community analysis that would not have been possible using bulk stable isotope analyses alone due to differences in isotopic baselines.     

Marine Anticancer Agents: An Overview with a Particular Focus on Their Chemical Classes

The marine environment is a rich source of biologically active molecules for the treatment of human diseases, especially cancer. The adaptation to unique environmental conditions led marine organisms to evolve different pathways than their terrestrial counterparts, thus producing unique chemicals with a broad diversity and complexity. So far, more than 36,000 compounds have been isolated from marine micro- and macro-organisms including but not limited to fungi, bacteria, microalgae, macroalgae, sponges, corals, mollusks and tunicates, with hundreds of new marine natural products (MNPs) being discovered every year. Marine-based pharmaceuticals have started to impact modern pharmacology and different anti-cancer drugs derived from marine compounds have been approved for clinical use, such as: cytarabine, vidarabine, nelarabine (prodrug of ara-G), fludarabine phosphate (pro-drug of ara-A), trabectedin, eribulin mesylate, brentuximab vedotin, polatuzumab vedotin, enfortumab vedotin, belantamab mafodotin, plitidepsin, and lurbinectedin. This review focuses on the bioactive molecules derived from the marine environment with anticancer activity, discussing their families, origin, structural features and therapeutic use.