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81.
GhCesA2 is a cotton (Gossypium hirsutum) homologue of the bacterial cellulose synthase gene that encodes cellulose 4--glucosyltransferase. The central catalytic region of GhCesA2 was expressed as a soluble protein in the methylotrophic yeastPichia pastoris. The molecular size of the recombinant protein was 100kDa, which decreased to 85 kDa after treatment with endoglycosidase H. The recombinant GhCesA2 catalyzed transfer of glucose from UDP-glucose into unknown products in the presence of an extract of cotton hypocotyls, but the products were not-1,4-glucan. 相似文献
82.
Takumi MOTOYA Noriko NAGATA Harumi KOMORI Ikuko DOI Miho KUROSAWA Toshimasa KETA Nobuya SASAKI Koji ISHII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1705-1709
Hepatitis E virus (HEV) is known as a causative agent of zoonosis and food poisoning.
Pigs and some species of wild animals, including wild boar, are known to be a reservoir of
HEV. In this study, we investigated the situation regarding HEV infection in wild boars in
Ibaraki Prefecture, Japan. Serum, liver and feces samples from 68 animals were collected,
and the presence or absence of HEV genomic RNA and HEV antibodies were analyzed. The viral
genome was detected in samples from 7 (10.3%) animals, with all HEVs classified as
genotype 3, subtype 3b. HEV antibodies were detected in samples from 28 (41%) animals.
This report demonstrates for the first time the high prevalence of HEV infection in wild
boars in Ibaraki Prefecture. 相似文献
83.
Yasuharu Hoshino Hitoshi Yonenobu Koh Yasue Yoshihiro Nobori Takumi Mitsutani 《Journal of Wood Science》2008,54(3):183-188
We developed ring-width chronologies for living trees of Japanese beech at two forest sites on the northern-most part of Honshu
Island, Japan. A statistical threshold (running expressed population signal) yielded these site chronologies spanning 1853–1994
(142 years) and 1867–1994 (128 years). We examined two factors, climate and masting, that could affect the variations of radial
growth. The response function analysis revealed that the ring width correlated positively with July and August temperatures
of the previous growth year. The optimal radial growth of Japanese beech may largely depend on a warm previous summer with
above-average temperatures. The years of good masting coincided mostly with those showing abrupt growth depression, although
only the short-term records of masting were available.
Part of this work was presented at the 6th Pacific Regional Wood Anatomy Conference, Kyoto, December 2005, and the 7th International
Conference on Dendrochronology, Beijing, June 2006 相似文献
84.
Differences in rumen fermentation characteristics between low‐yield and high‐yield dairy cows in early lactation
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Ahmad Sofyan Makoto Mitsumori Hideyuki Ohmori Yutaka Uyeno Toshiya Hasunuma Kiyoshi Akiyama Hiroshi Yamamoto Hiroaki Yokokawa Tsuneko Yamaguchi Takumi Shinkai Makoto Hirako Shiro Kushibiki 《Animal Science Journal》2017,88(7):974-982
Relationship between rumen fermentation parameters, blood biochemical profiles and milk production traits in different yielding dairy cows during early lactation was investigated. Twelve dairy cows were divided into two groups based on their milk yield, that is low‐yield (LY) and high‐yield (HY) groups. Rumen fluid and blood were collected at 3 weeks prepartum and 4, 8 and 12 weeks postpartum. Results showed that proportions of acetate, propionate to total short chain fatty acids and acetate : propionate ratio were changed (P < 0.05) in both groups during the peripartum period, whereas butyrate and acetate : butyrate ratio were only altered in the HY group. Blood cholesterol, beta‐hydroxybutyric acid (BHBA) and glutamic oxaloacetic transaminase in the HY group were higher (P < 0.01) than those in the LY group. Principal component analysis revealed that milk yield and milk compositions were differently clustered between groups. These parameters showed similar direction with dry matter intake in the HY group and adverse direction in the LY group. Linear regression analysis indicated that butyrate was positively correlated with BHBA (P < 0.05) in the HY group. This study suggests that cows in the HY group seem to accommodate appropriately to negative energy balance in early lactation through rumen fermentation. 相似文献
85.
Okawa T Hiraoka H Wada Y Baba K Itamoto K Mizuno T Okuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):677-680
Second malignancies are frequent complications in human patients with chronic lymphocytic leukemia (CLL). However, the clinical details and outcome of this phenomenon were unclear in their canine counterparts. Here, we report a dog with high-grade lymphoma concurrent with T-cell CLL. A 10-year-old male golden retriever presented with lymphadenopathies. The lymph nodes contained large-sized lymphocytes, raising suspicion of high-grade lymphoma. Meanwhile, small lymphocytic lymphocytosis in the peripheral blood was consistent with CLL. Interestingly, molecular biological analyses revealed that CLL cells were of the T-cell type, whereas lymphoma cells were of the B-cell type. Chemotherapy using the L-VCA short protocol was effective for 155 days, but the dog died on day 194 after diagnosis, despite rescue therapies. 相似文献
86.
87.
Yasuyuki KANEKO Shidow TORISU Takumi KOBAYASHI Shinya MIZUTANI Nao TSUZUKI Hiroko SONODA Masahiro IKEDA Kiyokazu NAGANOBU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1633-1638
Arterial blood gas analysis is an important diagnostic and monitoring tool for
respiratory abnormalities. In human medicine, lung complications often occur as a result
of liver disease. Although pulmonary complications of liver disease have not been reported
in dogs, we have frequently encountered hypoxemia in dogs with liver disorders, especially
extrahepatic biliary obstruction. In addition, respiratory disorders account for 20% of
perioperative fatalities in dogs. Therefore, in this study, we evaluated the respiratory
status in dogs with hepatobiliary disease by arterial blood gas analysis. PaO2
and PaCO2 were measured. Alveolar-arterial oxygen difference
(AaDO2), the indicator of gas exchange efficiency, was calculated. Compared to
healthy dogs (control group), hepatobiliary disease dogs had significantly lower
PaO2 and higher AaDO2. Hypoxemia (PaO2 of ≤80 mmHg) was
observed in 28/71 dogs with hepatobiliary disease. AaDO2 was higher (≥30 mmHg)
than the control group range (11.6 to 26.4 mmHg) in 32/71 hepatobiliary disease dogs. By
classifying type of hepatobiliary disease, dogs with extrahepatic biliary obstruction and
chronic hepatitis showed significantly lower PaO2 and higher AaDO2
than in a control group. Dogs with chronic hepatitis also had significantly lower
PaCO2. The present study shows that dogs with hepatobiliary disease have
respiratory abnormalities more than healthy dogs. Preanesthetic or routine arterial blood
gas analysis is likely beneficial to detect the respiratory abnormalities in dogs with
hepatobiliary disease, especially extrahepatic biliary obstruction and chronic
hepatitis. 相似文献
88.
Kazuki Komatsu Tsugumi Iwasaki Kosuke Murata Hideaki Yamashiro Valerie Swee Ting Goh Ryo Nakayama Yohei Fujishima Takumi Ono Yasushi Kino Yoshinaka Simizu Atsushi Takahashi Hisashi Shinoda Kentaro Ariyoshi Kosuke Kasai Masatoshi Suzuki Maria Grazia Palmerini Manuel Belli Guido Macchiarelli Toshitaka Oka Manabu Fukumoto Mitsuaki A. Yoshida Akifumi Nakata Tomisato Miura 《Reproduction in domestic animals》2021,56(3):484-497
Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, we have established an archive system of livestock and wild animals from the surrounding ex-evacuation zone. Wildlife within the alert zone have been exposed to low-dose-rate (LDR) radiation for a long continuous time. In this study, we analysed the morphological characteristics of the testes and in vitro fertilization (IVF) capacity of cryopreserved sperm of racoons from the ex-evacuation zone of the FDNPP accident. The radioactivity of caesium-137 (137Cs) was measured by gamma-ray spectrometry, and the measured radioactivity concentration was 300–6,630 Bq/kg in the Fukushima raccoons. Notably, normal spermatogenesis was observed in the seminiferous tubules of the testes, with the germinal epithelium composed of a spermatogenic cell lineage with no evident ultrastructural alterations; freeze-thawing sperm penetration ability was confirmed using the interspecific zona pellucida-free mouse oocytes IVF assays. This study revealed that the chronic and LDR radiation exposure associated with the FDNPP accident had no adverse effect on the reproductive characteristics and functions of male raccoons. 相似文献
89.
Chunyan Liu Sanae Asano Hikonosuke Ogata Shun Ito Takumi Nakase Sui Takeda Kyougo Miyoshi Youichi Numata Kei Takahashi Hiroshi Kajikawa 《Animal Science Journal》2021,92(1):e13535
The intake, digestion, and ruminal fermentation properties; the antioxidative activity; and the physical effectiveness of pineapple residue (PR) from the cut-fruit industry as a feed for cattle were investigated. Four nonlactating Holstein cows were fed a basal diet and diets with 1.4- and 3.6-kg dry matter (DM)/day of PR (low-PR and high-PR diets, respectively). The DM digestibility and total digestible nutrients were 71% and 70%, respectively. Ruminal pH, volatile fatty acids, and ammonia-N did not differ among the diets. The numbers of total viable, amylolytic, cellulolytic, and methanogenic bacteria and protozoa, and microbial protein synthesis in the rumen also did not differ significantly. Blood triglyceride, nonesterified fatty acids, urea nitrogen, and aspartate transaminase were lower in the high-PR diet. Feeding PR did not affect blood malondialdehyde, although PR has a higher antioxidative value than other commonly used feeds. The particle distribution of PR satisfied the recommended range for haylage. The chewing time for ingestion and rumination did not change with PR content, and the roughage value index of PR was 57-min/kg DM. These results suggest that PR has good intake and digestion properties and satisfactory physical effectiveness. Even a high-PR-content feed unlikely induces the risk of ruminal acidosis. 相似文献
90.
Masaki Honda Yuki Kawashima Kazuaki Hirasawa Takeshi Uemura Jinkun Sun Yoshiaki Hayashi 《Animal Science Journal》2021,92(1):e13512
The effects of feeding diets containing astaxanthin with different Z-isomer ratios to laying hens on egg qualities, such as astaxanthin concentration in egg yolk and yolk color, were investigated. As the astaxanthin source, a natural microorganism Paracoccus carotinifaciens was used. Astaxanthin with different Z-isomer ratios was prepared by thermal treatment with different conditions and then added to the basal diet at a final astaxanthin concentration of 8 mg/kg. We found that, as the Z-isomer ratios of astaxanthin in the diet increased, the astaxanthin concentration in egg yolk and the yolk color fan score also increased significantly. Importantly, feeding a 50.6% Z-isomer ratio diet increased astaxanthin concentration in egg yolk by approximately fivefold and the color fan score by approximately 2 compared to that in hens fed an all-E-isomer-rich diet. Moreover, we showed that feeding Z-isomer-rich astaxanthin to laying hens increased plasma astaxanthin concentration by more than five times in comparison to that in hens fed an all-E-isomer-rich diet. These results indicate that Z-isomers of astaxanthin have higher bioavailability than that of the all-E-isomer and thus they exhibit greater egg yolk-accumulation efficiency. 相似文献