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281.
Seasonal variation in docosahexaenoic acid content in horse mackerel caught in the East China Sea 总被引:3,自引:0,他引:3
Kazufumi OSAKO Akira YAMAGUCHI Takao KUROKAWA Koichi KUWAHARA Hiroaki SAITO Yukinori NOZAKI 《Fisheries Science》2003,69(3):589-596
ABSTRACT: The fatty acid composition in horse mackerel caught off Nagasaki, off Tsushima Island, and in the middle of the East China Sea was investigated. The ratios of monoenoic and polyenoic acids to the total fatty acids in the Nagasaki and bull trawl catch specimens had a negative correlation. The docosahexaenoic acid (DHA; 22:6n-3) ratios of total fatty acids in summer-caught specimens were lower than those in winter-caught specimens, and this tendency was significantly clearer in smaller-sized fish, while DHA levels in tissues varied little throughout the year. Consistently high levels of DHA were found in various fishing areas, which suggests that horse mackerel offers a stable source of DHA. 相似文献
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Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in genebanks due their allogamous nature. Cryopreserved materials insure a long-term backup of field collections against loss of plant germplasm. Occurrence of genetic variation, in tissue culture cells during prolonged subcultures, can be avoided with suitable cryopreservation protocols that provide high regrowth, leading and facilitating a systematic and strategic cryo-banking of plant genetic resources. Cryopreservation protocols for potato reviewed here, can efficiently complement field and in vitro conservation, providing for preservation of genotypes difficult to preserve by other methods, wild types and other species decided as priority collections. 相似文献
285.
Koji MISUMI Yuri HIRAYAMA Sachiko EGAWA Shoko YAMASHITA Hiroyoshi HOSHI Kei IMAI 《The Journal of reproduction and development》2013,59(6):520-524
This study was conducted to clarify the feasibility of newly developed vitrification
techniques for porcine embryos using the micro volume air cooling (MVAC) method
without direct contact with liquid nitrogen (LN2). Expanded blastocysts
were vitrified in a solution containing 6 M ethylene glycol, 0.6 M trehalose and 2%
(wt/vol) polyethylene glycol in 10% HEPES-buffered PZM-5. The blastocysts were
collected from gilts and vitrified using the new device (MVAC) or a Cryotop (CT).
Blastocysts were stored in LN2 for at least 1 month. After warming,
cryoprotective agents were removed using a single step. Survival of the embryos was
assessed by in vitro culture (Experiment 1) and by embryo transfer
to recipients (Experiment 2). In Experiment 1, the embryos vitrified by the MVAC or
CT and fresh embryos without vitrification (Control) were used. The survival rates of
embryos in the MVAC, CT and Control groups were 88.9% (32/36), 91.7% (33/36) and 100%
(34/34), respectively, after 48 h culture, and the hatching rates of embryos after 48
h incubation were 69.4% (25/36), 63.9% (23/36) and 94.1% (32/34), respectively. In
Experiment 2, 64 vitrified embryos were transferred to 5 recipient gilts, and 8
healthy piglets were produced from 3 recipients in the MVAC group. Similarly, 66
vitrified embryos were transferred to 5 recipient gilts, and 9 healthy piglets were
produced from 2 recipients in the CT group. These results indicated that porcine
expanded blastocysts can be cryopreserved using the MVAC method without potential
pathogen contamination from LN2. 相似文献
286.
Sheng-An Tang Qianxiang Zhou Wen-Zhi Guo Yuling Qiu Ran Wang Meihua Jin Wenjing Zhang Ke Li Takao Yamori Shingo Dan Dexin Kong 《Marine drugs》2014,12(7):4200-4213
Stellettin B was isolated from marine sponge Jaspis stellifera. In vitro antitumor activities were investigated on 39 human cancer cell lines. Stellettin B exhibited highly potent inhibition against the growth of a human glioblastoma cell line SF295, with a GI50 of 0.01 μM. In contrast, stellettin B showed very weak inhibitory activity on normal cell lines including HMEC, RPTEC, NHBE and PrEC, with GI50s higher than 10 μM, suggesting its relatively selective cytotoxicity against human cancer cells compared to normal human cell lines. We then focused on the antitumor activity of this compound on SF295 cells. Flow cytometric analysis indicated that stellettin B induced apoptosis in SF295 cells in a concentration-dependent manner. Further study indicated that stellettin B increased the production of ROS, the activity of caspase 3/7, as well as the cleavage of PARP, each of which is known to be involved in apoptosis. To investigate the molecular mechanism for cell proliferation inhibition and apoptosis induction, effect on the phosphorylation of several signal proteins of PI3K/Akt and RAS/MAPK pathways was examined. Stellettin B inhibited the phosphorylation of Akt potently, with no activity on p-ERK and p-p38, suggesting that inhibition of PI3K/Akt pathway might be involved in the antiproliferative and apoptosis-inducing effect. However, homogenous time-resolved fluorescence (HTRF) assay indicated that stellettin B did not inhibit PI3K activity, suggesting that the direct target might be signal protein upstream of Akt pathway other than PI3K. 相似文献
287.
288.
Kazufumi OSAKO Akira YAMAGUCHI Takao KUROKAWA Koichi KUWAHARA Hiroaki SAITO Yukinori NOZAKI 《Fisheries Science》2002,68(3):587-594
ABSTRACT: The chemical components and body color of horse mackerel caught between July 1997 and May 2000 were studied. The fish were caught with small- to medium-sized purse seine offshore from Nagasaki and from Tsushima, and with bull trawl seine and medium- to large-sized purse seine in the East China Sea. The crude lipid content of the fish caught offshore from Tsushima was higher than that of the others, and there were no significant differences among the other catches. The extractive nitrogen content of the fish caught with bull trawl seine was somewhat lower than that of the others. The body colors of the fish differed from those suggested by their common names: Kuroaji (black) , Kiaji (yellow), and Shiroaji (white). The crude lipid content and body color indicated that there was no difference in quality among the catches, with the exception of the crude lipid content of the Tsushima catch. 相似文献
289.
Hieu Duc DUONG Yuji TANIGUCHI Yasuhiro TAKASHIMA Satoshi SEKIGUCHI Khin Myo AYE Parnian AHMADI Linh Khanh BUI Takao IRIE Eiji NAGAYASU Ayako YOSHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(7):905
Toxoplasmosis is a widespread protozoan zoonosis. Since ingesting undercooked meat harboring Toxoplasma gondii cyst is considered one of the major transmission routes to humans, the screening of T. gondii in meat-producing animals can reduce the risk of food-borne toxoplasmosis in humans. Among serological diagnostic methods, Luciferase-linked Antibody Capture Assay (LACA) has been found to be a promising platform with high sensitivity and specificity. In this study, we aimed to evaluate recombinant nanoluciferase fused-T. gondii antigens (rNluc-GRA6, rNluc-GRA7, rNluc-GRA8 and rNluc-BAG1) for their potential use in LACA for pigs. As a result, the sensitivity of GRA6-, GRA7-, GRA8- and BAG1-LACA were 70.0%, 80.0%, 80.0% and 30.0% with specificity 87.0%, 81.5%, 74.1% and 50.0%, respectively. The cocktail LACA using a mixture of rNluc-GRA6, rNluc-GRA7 and rNluc-GRA8 indicated higher sensitivity (90.0%) and a similar specificity (96.3%) in comparison with the commercial ELISA kit. Compared to the Dye-Test as a reference test, cocktail LACA showed strong agreement (kappa value=0.811) when we assessed pig sera collected at the slaughterhouse. In addition, we also successfully established the rapid LACA format for the detection of Toxoplasma infection in pigs (called Rapid-LACA) in which the test could be performed within 30 min. In Rapid-LACA, the protein A pre-coated/blocked plates could be preserved at −30°C, 4°C or room temperature conditions for at least two months without compromising on the quality of assay. 相似文献
290.
Kiwamu HANAZONO Mako NAKAMOTO Ai HORI Kenjiro MIYOSHI Tetsuya NAKADE Takaharu ITAMI Tadashi SANO Keiko KATO Akifumi ITO Kakeru TANAKA Kazuto YAMASHITA Daiji ENDOH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(12):1556
This study investigated the association between caudal vena cava (CVC) size and circulatory dynamics in dogs using computed tomography (CT) under general anesthesia. The subjects were 104 dogs who had undergone CT under general anesthesia in the past. The ratio of short diameter of the CVC to aortic diameter (CVCS/Ao) and the ratio of long to short diameter of the CVC (CVCL/CVCS) in the thorax and abdomen, respectively, were calculated using factors such as mean blood pressure (MBP), shock index (SI), anemia, hypoproteinemia, presence of intra-abdominal mass, and cardiac disease. There was a significant but negligible negative correlation between CVCS/Ao and MBP. In contrast, no significant correlation was found between CVC size and SI. The low MBP group had significantly higher CVCS/Ao of the thorax than the normal MBP group. The group with intra-abdominal mass had significantly lower CVCS/Ao of the abdomen than the group without intra-abdominal mass. The group with cardiac disease had significantly lower CVCL/CVCS of the thorax than the group without cardiac disease. In multiple regression analysis, low MBP, cardiac disease, intra-abdominal mass, and anemia were significant factors for CVCS/Ao of the thorax, CVCL/CVCS of the thorax, CVCS/Ao of the abdomen, and CVCL/CVCS of the abdomen, respectively. In conclusion, CVC size assessment using CT in dogs under general anesthesia is influenced by various factors. 相似文献