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排序方式: 共有458条查询结果,搜索用时 15 毫秒
91.
Harada Kousuke Kato Yuria Takahashi Jun Imamura Hiyoko Nakamura Natsume Nishina Atsuyoshi Phay Nyunt Tadaishi Miki Shimizu Makoto Kobayashi-Hattori Kazuo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):103-109
Plant Foods for Human Nutrition - Adipose tissue is an endocrine organ and its endocrine function is closely associated with type 2 diabetes mellitus. Valeriana officinalis (Valerian) exerts some... 相似文献
92.
Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates 下载免费PDF全文
Keita Nishiyama Shintaro Ueno Makoto Sugiyama Yuji Yamamoto Takao Mukai 《Animal Science Journal》2016,87(6):809-815
Lactobacillus rhamnosus GG (LGG) is a well‐established probiotic strain. The beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. The pilin SpaC subunit, located within the Spa pili structure, is the most well studied LGG adhesion factor. However, the binding epitopes of SpaC remain largely unknown. The aim of this study was to evaluate the binding properties of SpaC to the carbohydrate moieties of intestinal glycoconjugates using a recombinant SpaC protein. In a competitive enzyme‐linked immunosorbent assay, SpaC binding was markedly reduced by addition of purified mucin and the mucin oligosaccharide fraction. Histochemical staining revealed that the binding of SpaC was drastically reduced by periodic acid treatment. Moreover, in the surface plasmon resonance‐based Biacore assay, SpaC bound strongly to the carbohydrate moieties containing β‐galactoside at the non‐reducing terminus of glycolipids. We here provide the first demonstration that SpaC binds to the oligosaccharide chains of mucins, and that the carbohydrate moieties containing β‐galactoside at the non‐reducing termini of glycoconjugates play a crucial role in this binding. Our results demonstrate the importance of carbohydrates of SpaC for mucus interactions. 相似文献
93.
Shyamal C MAHATA Ryoichi MITSUO Jun-Ya AOKI Hironori KATO Takao ITAKURA 《Fisheries Science》2003,69(3):615-624
ABSTRACT: The cytochrome P450 (CYP) represents a large group of microsomal monooxygenases that catalyze drugs as well as a host of lethal environmental contaminants such as dioxins, leading to either detoxification and excretion from the animal or generation of carcinogenic intermediates. In the present study two forms of cDNA were cloned (Eu MC1 and Eu MC2) for European eel CYP1A genes by polymerase chain reaction (PCR) techniques. The cDNA of Eu MC1 was 3368 bp long coding 521 amino acid residues, and that of Eu MC2 was 2464 bp long coding 517 amino acid residues. Identities of deduced amino acid sequences between Eu MC1 and Japanese eel CYP1A1 and that between Eu MC2 and the second form of Japanese eel CYP1A were 98% and 97%, respectively, showing decisively that Eu MC1 and Eu MC2 are orthologous to Japanese eel CYP1A1 and the second form of CYP1A, respectively. A striking difference between the two eel species was that the Eu MC1 peptide was two amino acid residues longer than that of the Japanese eel CYP1A1. Existence of two loci of CYP1A in Japanese and European eels may suggest that the two forms of CYP1A exist widely among the eel species, because the divergence between the two eel species has been shown to be close to the basal divergence among eels. The identities in CYP1A may help to estimate genetic distance between European and Japanese eels. 相似文献
94.
95.
Inhibitory influence of inorganic salts on banana postharvest pathogens and preliminary application to control crown rot 总被引:4,自引:0,他引:4
Dionisio G.?Alvindia Takao?Kobayashi Keiko T.?NatsuakiEmail author Seinosuke?Tanda 《Journal of General Plant Pathology》2004,70(1):61-65
Lasiodiplodia theobromae, Thielaviopsis paradoxa, Colletotrichum musae, C. gloeosporioides, Fusarium verticillioides, and F. oxysporum were screened in vitro for sensitivity to Na2CO3, NaHCO3, CaCl2, NaCl, and NaClO. The spore germination of all pathogens was completely inhibited by Na2CO3 4g/l, NaClO 5g/l, and NaHCO3, CaCl2, and NaCl 6g/l each. Dipping the bananas for 10–15min in these concentrations reduced the incidence of crown rot (compared with the untreated fruits) 17 days after harvest in fruits treated with NaClO by 67%, with NaHCO3 by 62%, with NaCl by 38%, and with CaCl2 by 33%. Na2CO3-treated fruits had the same incidence of crown rot as untreated fruits. 相似文献
96.
Kasumi SUDO Manabu YAMADA Mariko OCHIAI Shoko IWAMOTO Hajime SAKAKI Chiho KUSHIDA Maiko YAMASHITA Atsushi YAMAMOTO Takao NAGASAKA Mikio OIDEMIZU Akito SAITO Kinya YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):990
We evaluated the role of classical swine fever virus (CSFV) in the formation of button ulcers in the mucosa of the gastrointestinal tract. Histopathological and immunohistochemical analyses of pigs experimentally infected with a subgenotype 2.1 isolate of CSFV, which was isolated in Japan in 2019, revealed follicular necrosis in the submucosal mucosa-associated lymphoid tissue and herniation of crypts as factors that contribute to the development of button ulcers during CSFV infection. These findings indicate that CSFV induces follicular necrosis and is one of the causative agents of button ulcers in pigs. 相似文献
97.
Hirano M Watanabe K Murakami M Kano R Yanai T Yamazoe K Fukata T Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(3):283-284
We excised surgically a feline granulomatous lesion and performed histopathological, mycological and molecular examinations. As a result, it was diagnosed as sporotrichosis, which was the second recorded case of a cat so afflicted in Japan. After the operation, we recognized another nodule on the lymph node. Histopathological examination was therefore performed, but no fungi were detected. To prevent recurrence, the cat was administered a antimycotic drug, itraconazole. As a result, no recurrence was found. Excision of the lesion is the treatment of choice for feline sporotrichosis. 相似文献
98.
Ishii N Nakahigashi K Baba T Robert M Soga T Kanai A Hirasawa T Naba M Hirai K Hoque A Ho PY Kakazu Y Sugawara K Igarashi S Harada S Masuda T Sugiyama N Togashi T Hasegawa M Takai Y Yugi K Arakawa K Iwata N Toya Y Nakayama Y Nishioka T Shimizu K Mori H Tomita M 《Science (New York, N.Y.)》2007,316(5824):593-597
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations. 相似文献
99.
Innami Satoshi Tabata Kimiko Shimizu Jun Kusunoki Kikue Ishida Hiroshi Matsuguma Miki Wada Masahiro Sugiyama Noriko Kondo Mika 《Plant foods for human nutrition (Dordrecht, Netherlands)》1998,52(1):55-66
A cholesterol-free diet containing dried powder of Jew's mellow leaves, persimmon leaves or sweet potato leaves respectively at 5% level as dietary fiber was fed to male Sprague-Dawley rats for about one month. The experiment was conducted twice except for sweet potato leaves. In the groups fed the diet mixed with powders of any of the three different dried green leaves, the hepatic cholesterol concentration significantly decreased. Such lowering was not observed in serum cholesterol concentration compared with the control (cellulose) group. A significant increase in fecal weight was observed in all the groups fed the green leaf samples. All the dried green leaves increased fecal excretion of bile acids per gram or per day compared with the control group in both experiments, but only the dried Jew's mellow leaves showed an increased excretion of neutral sterols. These results suggest that lowering of hepatic cholesterol by powdered green leaves is not necessarily due to the same factor, but to the increased fecal excretion of bile acids due to inhibited enterohepatic circulation in animals given these samples. 相似文献
100.
Ko Fujioka Ryo Kawabe Alistair J. Hobday Yoshimi Takao Kazushi Miyashita Osamu Sakai Tomoyuki Itoh 《Fisheries Science》2010,76(3):403-410
Acoustic tags were used to examine the spatial and temporal distribution of southern bluefin tuna (SBT) in southern Western Australia, which is in a region where fishery-independent acoustic surveys of the recruitment abundance index of SBT have been historically undertaken. We investigated patterns of SBT distribution within and inshore of the acoustic survey area during three summer seasons. Annual differences in distribution patterns were characterized by two distinctive migration pathways. An inshore-migrating pathway was observed in two seasons (2004/2005 and 2006/2007), with a relatively high proportion of tagged SBT (84.5, 65.0%) migrating inshore of the acoustic survey area. The other pathway was concentrated along the shelf (2005/2006 season), with an estimated 63.3% of tagged SBT moving within the survey area. These variable migration patterns may bias the interannual fluctuations in abundance indices. Current survey methods can be modified to include both inshore and continental shelf areas. This contribution shows that the accuracy of acoustic surveys can be improved by including ecological patterns. 相似文献