Validation of multi-frequency inversion method by using dummy scatterers of zooplankton

ABSTRACT:   High- and multi-frequency acoustic measurement systems and the multi-frequency inversion (MFI) method have been used to measure spatial distributions and abundances of zooplankton by size. In this study, the calibration method for high- and multi-frequency systems was developed and the validation of MFI method was carried out by scatterer measurement. The standard sphere calibration method that has not been applied to such high- and multi-frequencies was applied to calibrate our high- and multi-frequency system, TAPS-6 (Tracor Acoustic Profiling System, BAE Systems). An optimum size of standard sphere of tungsten carbide of 1 mm radius was derived to have a small target strength variation for the six frequencies of TAPS-6, and the practicability and precision of the standard sphere calibration method was confirmed for those frequencies. A school or cluster of dummy scatterers of zooplankton with small tungsten carbide spheres were designed to validate the MFI method, and volume back-scattering strength values were measured by the multi-frequency system. By comparing the result of the inversion with their real composition, the features of the MFI method could be validated and examined.     

Developing fertilizer recommendations for rice in Sub-Saharan Africa,achievements and opportunities

Improving agricultural productivity to keep pace with the fast-growing food demand is a huge challenge for sub-Saharan Africa (SSA). Fertilizer is a powerful productivity-enhancing input; nevertheless, farmers of SSA use only 5–9 kg ha^?1 of fertilizer, which is ten times lesser than Latin America and Asia (50 and 80 kg ha^?1, respectively). Rice (Oryza sativa) is one of the most important food crops of SSA, and its consumption is growing faster than any other commodity in Africa. Rice-based systems have high potential for improving food production through an efficient management of fertilizers. The biophysical environment, cropping systems and socio-economic status of farmers including market opportunities are the main factors for developing appropriate fertilizer recommendations. Many research efforts have been invested in different countries to develop fertilizer recommendation for rice. However, the diversity of rice ecologies, the type and the cost of fertilizers available on local market are the main constraints for development of blanket recommendations of fertilizer usually applied in many countries. Here, we make a reviews of the progress made on the development of fertilizer recommendations for rice-based systems in SSA. The utilization of the new concepts and decisions support tools for development of fertilizer recommendation and the main achievements and weakness are discussed. The opportunities offered by the new concepts, modeling and decision support tools are discussed in a regional strategic approach for better management of fertilizers in the diversified ecologies of rice-based systems.     

Nomenclature and genetic relationships of apples and pears from Terceira Island

Heritage apple (Malus domestica Borkh. hybrids) and pear (Pyrus communis L. hybrid) trees grow in villages throughout Terceira Island, Azores, Portugal. Some of these pears have different names but similar morphology. The objective of this study was to determine synonymy, homology, and phylogeny of apples and pears from Terceira and to examine potential relationships of the island pears with standard apples and pears of Portuguese or American descent. Nine apple microsatellite markers were used to determine genetic relationships. Distance- and parsimony-based cluster analysis grouped these genotypes into separate apple and pear clades. The Terceira apples were divided into two clades: the maçā and the reineta-reinette. Among the 17 heritage apple genotypes, seven unique accessions were identified and four groups of synonyms, or possibly clones, were detected including: ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares; ‘Reineta Castanha’ and ‘Reineta Verde Miuda’; ‘Maçā Pêra,’ ‘Maçā Calhau’, ‘Pêro Branco’ from Salga and from Terra-Chā and ‘Maçā Marmelo’; and the five genotypes ‘Maçā Sao Joao’, ‘Malápio Rosa’, ‘Maçā Gaspar’, ‘Maçā Branca’ and ‘Maçā Pato’. In addition, two homonyms were detected. ‘Pêro Vermelho’ from Terra Chā was a separate genotype from a tree from Doze Ribeiras of the same name, but Pêro Branco from Terra Chā appears to be a clone that can be distinguished by an additional allele at CH1F07a from a tree with that name from Salga. One pair of apple clones, ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares appear to be derived from an unreduced gamete of ‘Golden Delicious.’ Another apple genotype ‘Maçā Acida’ could be a sibling of the ‘Maçā Pêra’ clonal group. Other tested standard apples from the US genebank were unrelated to Terceira genotypes. Of the seven heritage pears, five unique genotypes and one pair of synonyms were detected. ‘Pêra Papo Pintassilgo’ from Raminho and ‘Pêra Vermelha’ from the nursery of Serviço de Desenvolvimento Agario da Terceira (SDAT) were synonyms. ‘Passans du Portugal’ was related to ‘Pêra Cabaca’ but other standard pears from the US genebank were unrelated to Terceira genotypes. Future studies will include additional apple and pear cultivars from other Islands of the Azores and continental Portugal, and wild Asian species to further explore genetic relationships.     

Reputational damage and the Fukushima disaster: an analysis of seafood in Japan

As the levels of radioactivity in seafood have fallen back into the safe range, Fukushima fisheries are considering reopening. However, even if seafood from the Fukushima area were sufficiently safe to distribute to seafood markets, its value may be undermined because of the damage done to its reputation by the Fukushima disaster. We quantified consumers’ preferences for seafood from Fukushima and adjacent prefectures to examine the extent of the reputational damage to Fukushima seafood. We conducted a choice experiment to measure consumers’ willingness to pay for seafood from the Fukushima area. We also measured the impact of displaying ecolabels [Marine Stewardship Council (MSC) and Marine Eco-label Japan (MEL)] on Fukushima products. The results indicated that Fukushima products are considerably discounted compared with products displayed as domestic; even products from adjacent prefectures are substantially discounted. By contrast, consumers positively evaluated locally labeled products. We also found that demersal fish are discounted more than pelagic fish that inhabit the ocean surface off the shore of Fukushima.     

Regional phenotypic diversity of Puccinia triticina and wheat host resistance in western Europe, 1995

Pathogenicity data from surveys of Puccinia triticina (formerly P. recondita f. sp. tritici) conducted in western Europe in 1995 were analysed to compare the structure of regional populations of the pathogen. Many of the populations differed in phenotypic diversity and pathotypic composition, even though they occurred within a single epidemiological unit, suggesting that local factors may influence the establishment and propagation of individual pathotypes in the regional populations. Neighbouring regions were more similar than distant regions, and all regions shared at least one pathotype, except populations in northern Italy and Scotland. A high degree of similarity was found between populations in northern France and Great Britain, providing strong evidence of free movement of inoculum between these regions. Resistance genes were postulated for a selection of 91 wheat cultivars, representing those most commonly grown in western Europe in 1995. Thirteen cultivars lacked detectable seedling resistance genes and the remaining 78 possessed from one to three resistance genes; those detected were Lr1, Lr3a, Lr10, Lr13, Lr14a, Lr17b, Lr20, Lr26 and Lr37. The most commonly detected resistance gene was Lr13, which was present singly or in combination with other resistance genes in 48 cultivars (53%). The gene Lr14a was detected in 18 cultivars, Lr26 was present in 16 cultivars. The role of host selection in the composition of the regional populations of P. triticina in western Europe in 1995 was difficult to assess on the basis of the results obtained, since virulence data were not available for Lr13 and Lr14a. This revised version was published online in August 2006 with corrections to the Cover Date.     

Regulation of amino acid biodegradation in soil as affected by depth

Dissolved organic nitrogen (DON) and in particular free amino acids represent a key pool in the terrestrial soil C and N cycle. The factors controlling the rate of turnover of this pool in soil, however, remain poorly understood. We investigated the factors regulating the rate of amino acid turnover at different depths (up to 1.2 m) in five low-input, acid soil profiles. Within the root zone (0–60 cm), amino acids constituted 8% of the DON and represented only a small fraction of plant available N. In all the soil profiles, the rate of amino acid mineralisation decreased progressively with depth. The average half-life of the exogenously added amino acids in the soil was 5.8 h in topsoils (0–10 cm), falling to 20 h at a depth of 50 cm and to 33 h at 100 cm. Generally, the rate of amino acid mineralisation correlated positively with total soil C and N, soil microbial activity (basal soil respiration rate) and soil content. The relatively rapid rates of microbial amino acid assimilation in subsoils below the root zone (>60 cm) indicate that long-term transport of amino acids (e.g. from soil to freshwaters) will be low. Based upon the C-to-N ratio of the amino acid substrate and the microbial C assimilation efficiency, we estimate that approximately 40–60% of the amino acid-N will be excreted as . In conclusion, the rapid rate of free amino acid turnover and their low concentration in soil solution indicate that the formation of inorganic N ( and ) in soil is limited primarily by the rate of free amino acid appearance in soil and not by the rate of amino acid mineralisation.     

Assessment of household risk management strategies for coastal aquaculture: the case of clam farming in Thaibinh Province,Vietnam

Clam farmers have experienced different types of risks that have been further exacerbated by the rapid expansion of clam farming areas, increased growing densities, and increased market difficulties in recent years in the Thaibinh Province of Vietnam. Most farmers have been seriously affected by production risk, market risk, and financial risk, while a number of others have met with success in almost all of their clam-raising cycles. This study applied a differentiating comparative analysis method and multiple discriminant analysis method to discuss the differences in risk management strategies between and among clam farming households and the impacts of those differences on their success/failure rates. In general, the tactics are related to increase in farm size, the application of technical innovations, diversifying livelihood activities, and accessing secure financial sources all provided better conditions for clam growth, diminished losses, and led to speedier recovery from shocks. To support farmers in managing risks, several government interventions are needed: (1) better re-zoning of clam farming areas in parallel with an increase in the farm size of each household, (2) promoting sustainable linkages between the farmers and the formal financial market and output market, and (3) investing more funding into research and extension related to sustainable clam farming practices and to the improvement of farmers’ skills in cooperative works and management.     

Water use in rice crop through different methods of irrigation in a sodic soil

Sodic soils are characterized by high exchangeable sodium on exchange sites, soil pH greater than 8.5, relatively low electrical conductivity, low infiltration rate and dispersed clay. These characteristics restrict the capacity of soil to absorb water, resulting in poor infiltration. Evidently, these soils require application of irrigation water at shorter intervals for crop production. Thus, irrigation strategy for sodic soils differs from that of normal soils. An experiment to determine the suitable irrigation strategy along with methods of application namely: surface (farmer’s practice), sprinkler (double nozzle impact sprinkler), and low-energy water application device (LEWA) were initiated in the year 2012 for rice crop. Irrigation depths of 6 cm in case of surface method and 4 cm in case of sprinkler and LEWA methods were applied at each irrigation event. The irrigation events for rice were scheduled at 2-DAD (days after the disappearance of the ponded water), 3-DAD, and 4-DAD through surface method, and at daily, 1- and 2-day intervals (after initial ponding disappeared) by sprinkler and LEWA methods. Sprinkler and LEWA methods resulted in highest rice yield of 4.4 t ha^?1 in irrigated plots at the 2-day interval which was at par with the highest yielding surface-irrigated plot scheduled at 2-DAD. At the same time, irrigation strategy of 2-day interval through sprinkler and LEWA methods registered water saving to the extent of 30–40% over 2-DAD under surface irrigation method. Results revealed that there could be substantial saving of water and energy (electricity and diesel) through the use of sprinkling devices for irrigating rice under sodic soil environments.     

Haemato-immunology and histo-architectural changes in <Emphasis Type="Italic">Labeo rohita</Emphasis> fingerlings: effect of dietary aflatoxin and mould inhibitor

A feeding trail of 60 days was conducted for delineating the effect of dietary aflatoxin (AFB1) with or without supplementation of a mixture of mould inhibitor (0.25% clove oil + 0.32% sodium propionate) on haematology, respiratory burst activity and histology of Labeo rohita fingerlings. Three hundred and sixty fishes (avg. wt. 1.48–1.54 g) were randomly distributed into eight treatment groups. Eight experimental diets with four different levels of aflatoxin (0, 10, 20 and 40 ppb) with or without mould inhibitor were prepared. Haematological parameters like total serum protein, albumin, globulin and A:G ratio were significantly (P < 0.05) reduced with increasing levels of aflatoxin in the diet. However, supplementation of mould inhibitor showed enhanced values when compared to non-supplemented counter parts suggesting ameliorating effects of mould inhibitor on aflatoxin. Total leucocyte count was higher in aflatoxin-treated groups. Histological observations were complementary to haematological parameters. Respiratory burst activity was significantly (P < 0.05) decreased in higher aflatoxin-treated groups but not affected significantly (P > 0.05) due to inclusion of inhibitor alone and/or interaction of aflatoxin level and inhibitor in the diet. From this study, it was concluded that up to 20 ppb aflatoxin level in the diet the haemato-immunological parameters are protected.     

Presence of less-preferred hosts of the aphid parasitoids <Emphasis Type="Italic">Aphidius ervi</Emphasis> and <Emphasis Type="Italic">Praon volucre</Emphasis> reduces parasitism efficiency

Parasitoids are characterized by a defined range of hosts, either more specialist or generalist. Under natural conditions, females may encounter different host species on the same plant or in the same location. In this case, their preference for one host could influence their choice. However, the presence of less suitable hosts may also affect their choice and, in some cases, may reduce their interest in a patch where both preferred and less preferred hosts are available. The aim of the present study was to test the consequences of the simultaneous presence of three cereal aphids (Sitobion avenae Fabricius, Metopolophium dirhodum Walker, and Rhopalosiphum padi Linnaeus) on the parasitism by two of their parasitoids, Aphidius ervi Haliday and Praon volucre Haliday. Firstly, in the no-choice experiment, A. ervi parasitized on S. avenae at a significantly higher rate as compared to M. dirhodum, whereas no parasitism on R. padi was observed. P. volucre parasitized the three species of cereal aphids with a significant preference for S. avenae. Interestingly, when two or three host species were offered simultaneously in the same quantity to pairs of parasitoids, the level of parasitism was less than that observed for one host species alone. This observation exhibits a distractive effect on non-host species, from the defense mechanism of a non-suitable host or from the perception of bad quality patches. These results raise the question of the practical application of inundative release of parasitoids for biocontrol when several hosts are available simultaneously.