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TAN Wei-ping XIA Yan WU Bao-jing LI Jing HUANG Hua-rong HUANG Shao-liang MAI Xian-di 《园艺学报》2009,25(12):2399-2402
AIM: To investigate the effect of T-bet plasmid gene transfer to airway on allergen induced airway inflammation in a murine asthmatic model. METHODS: A mouse asthma model was established by sensitization with ovalbumin (OVA). Forty C57BL/6 mice were divided into 4 groups (10 mice in each group): the normal control group (group A), the asthmatic model group (group B), the pcDNA3 plasmid group (group C), and the pcDNA3-T-bet group (group D). The animals in group B, C and D were sensitized and challenged with OVA. The animals in group A were applied with normal saline. pcDNA3 plasmid at dose of 50 μg was intranasally administered at 24 h before intranasal challenges to the mice in group C, and the 50 μg pcDNA3-T-bet plasmid for the mice in group D. Bronchial alveolar lavage fluid (BALF) was collected and lung tissues were resected at 48 h after OVA challenge for later assay. RESULTS: After administration with pcDNA3-T-bet plasmid, high level of T-bet expression at 48 h was detected in the lung tissue by Western blotting. In pcDNA3-T-bet treated asthmatic models, histological evaluation revealed the significant suppression of eosinophil peribronchial and perivascular infiltration, and reduction of epithelial damage. The numbers of eosinophils, neutrophils and lymphocytes in BALF from pcDNA3-T-bet treated mice were significantly reduced compared to those in asthmatic control group (P<0.05). The level of IL-4 in BALF was significantly decreased in pcDNA3-T-bet group compared to that in asthmatic control group (P<0.05), while the level of IFN-γ in BALF was significantly increased in pcDNA3-T-bet group. No significant change of inflammation cells and cytokines in pcDNA3 plasmid group and asthmatic control group was observed (P>0.05). CONCLUSION: Intranasal pcDNA3-T-bet plasmid transfer inhibits asthmatic airway inflammation in the murine asthmatic model, suggesting a new therapeutic strategy for allergic asthma. 相似文献
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在2006年3月22日至4月6日开展的太浦河调水试验中,实施水量水质同步监测的同时,进行了同位素监测.在太浦闸闸供和泵供两种不同调度方式下,对黄埔江上游水源地3条主要支流的18O同位素与水质指标的分别进行了关联分析.分析结果表明,泵供期关联度普遍高于闸供期,无论闸供期和泵供期,CODMn与δ18O的关联度都最高,NH3-N与δ18O的关联度最低.结果表明,泵供期对关联度的影响较为明显.CODMn、CODcr、TP以及TN均与δ18O有着较高的关联度,存在着明显的相关关系.δ18O同水质指标的灰色关联分析只是初步的探讨,还有待于深入的研究. 相似文献
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半干旱区甜菜水分生产函数试验研究 总被引:1,自引:0,他引:1
为了研究甜菜的耗水规律和节水方法以达到在半干旱区大面积推广的目的,在具有典型半干旱区气候特征的甘南县,以不同生育期的土壤水分水平作为控制的纸筒甜菜盆栽试验,对甜菜蒸腾量和产量之间的函数关系和生育期耗水规律进行了研究,得出了不同生育期甜菜蒸腾量和产量之间的函数关系.采用Jensen模型进行了水分生产函数的求解,建立了甜菜的水分生产函数模型.试验结果表明,甜菜各个生育期耗水量从小到大依次是苗期、糖分积累期、叶丛生长期和块根增长期,需水高峰期在7月和8月;水分敏感指数的变化规律与甜菜的耗水规律相一致,叶丛生长期的λ值最大,说明这个时期甜菜对水分的需求特别敏感,应该重点进行补水灌溉. 相似文献
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在澳期间通过听专家专业授课、专题访问、参观、调查等活动,学习考察了澳大利亚关于水资源管理与利用、节水措施、土壤改良技术应用、风力提水技术应用、集雨技术应用、覆盖技术应用、棉花滴灌技术应用、滴灌系统过滤装置等方面应用管理情况. 相似文献
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A 10‐week feeding experiment was conducted to evaluate the effect of different protein to energy ratios on growth and body composition of juvenile Litopenaeus vannamei (initial average weight of 0.09 ± 0.002 g, mean ± SE). Twelve practical test diets were formulated to contain four protein levels (300, 340, 380 and 420 g kg?1) and three lipid levels (50, 75 and 100 g kg?1). Each diet was randomly fed to triplicate groups of 30 shrimps per tank (260 L). The water temperature was 28.5 ± 2 °C and the salinity was 28 ± 1 g L?1 during the experimental period. The results showed that the growth was significantly (P < 0.05) affected by dietary treatments. Shrimps fed the diets containing 300 g kg?1 protein showed the poorest growth. However, shrimp fed the 75 g kg?1 lipid diets had only slightly higher growth than that fed 50 g kg?1 lipid diets at the same dietary protein level, and even a little decline in growth with the further increase of dietary lipid to 100 g kg?1. Shrimp fed the diet with 420 g kg?1protein and 75 g kg?1 lipid had the highest specific growth rate. However, shrimp fed the diet with 340 g kg?1 protein and 75 g kg?1 lipid showed comparable growth, and had the highest protein efficiency ratio, energy retention and feed efficiency ratio among dietary treatments. Triglycerides and total cholesterol in the serum of shrimp increased with increasing dietary lipid level at the same dietary protein level. Body lipid and energy increased with increasing dietary lipid level irrespective of dietary protein. Results of the present study showed that the diet containing 340 g kg?1 protein and 75 g kg?1 lipid with digestible protein/digestible energy of 21.1 mg kJ?1 is optimum for L. vannamei, and the increase of dietary lipid level has not efficient protein‐sparing effect. 相似文献