全文获取类型
收费全文 | 220篇 |
免费 | 13篇 |
专业分类
林业 | 7篇 |
农学 | 17篇 |
基础科学 | 1篇 |
75篇 | |
综合类 | 6篇 |
农作物 | 26篇 |
水产渔业 | 16篇 |
畜牧兽医 | 58篇 |
园艺 | 2篇 |
植物保护 | 25篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 14篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 9篇 |
2016年 | 7篇 |
2015年 | 16篇 |
2014年 | 8篇 |
2013年 | 11篇 |
2012年 | 15篇 |
2011年 | 22篇 |
2010年 | 12篇 |
2008年 | 18篇 |
2007年 | 15篇 |
2006年 | 11篇 |
2005年 | 9篇 |
2004年 | 9篇 |
2003年 | 5篇 |
2002年 | 6篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1989年 | 1篇 |
1973年 | 1篇 |
1971年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有233条查询结果,搜索用时 15 毫秒
61.
62.
Gianluca De Rinaldis Antonella Leone Stefania De Domenico Mar Bosch-Belmar Rasa Slizyte Giacomo Milisenda Annalisa Santucci Clara Albano Stefano Piraino 《Marine drugs》2021,19(9)
Increasing frequency of native jellyfish proliferations and massive appearance of non-indigenous jellyfish species recently concur to impact Mediterranean coastal ecosystems and human activities at sea. Nonetheless, jellyfish biomass may represent an exploitable novel resource to coastal communities, with reference to its potential use in the pharmaceutical, nutritional, and nutraceutical Blue Growth sectors. The zooxanthellate jellyfish Cassiopea andromeda, Forsskål, 1775 (Cnidaria, Rhizostomeae) entered the Levant Sea through the Suez Canal and spread towards the Western Mediterranean to reach Malta, Tunisia, and recently also the Italian coasts. Here we report on the biochemical characterization and antioxidant activity of C. andromeda specimens with a discussion on their relative biological activities. The biochemical characterization of the aqueous (PBS) and hydroalcoholic (80% ethanol) soluble components of C. andromeda were performed for whole jellyfish, as well as separately for umbrella and oral arms. The insoluble components were hydrolyzed by sequential enzymatic digestion with pepsin and collagenase. The composition and antioxidant activity of the insoluble and enzymatically digestible fractions were not affected by the pre-extraction types, resulting into collagen- and non-collagen-derived peptides with antioxidant activity. Both soluble compounds and hydrolyzed fractions were characterized for the content of proteins, phenolic compounds, and lipids. The presence of compounds coming from the endosymbiont zooxanthellae was also detected. The notable yield and the considerable antioxidant activity detected make this species worthy of further study for its potential biotechnological sustainable exploitation. 相似文献
63.
Perazzini R Leonardi D Ruggeri S Alesiani D D'Arcangelo G Canini A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(4):211-218
Eight Phaseolus vulgaris L. landraces cultivated on farm in marginal areas of Central Italy (Lazio region) were investigated in order to evaluate
chemical composition of storage proteins and secondary metabolites fractions. The total protein content showed some differences
among landraces; the maximum value was next to 30 g for 100 g of dry weight. The seed storage proteins were screened by polyacrylamide
gel electrophoresis (SDS/PAGE): seven landraces exhibited phaseolin patterns type S, one landrace showed a phaseolin pattern
type T. A morphological analysis of cotyledon parenchyma performed by scanning electron microscopy (SEM) revealed differences
in size of starch granules. Moreover the polyphenolic composition was investigated using HPLC-APCI; from the methanol extracts
a flavonoid, kaempferol, and a coumarin, 5,7-dimethoxycoumarin, were identified. To our knowledge, this is the first time
that 5,7-dimethoxycoumarin has been reported in P. vulgaris seeds. 相似文献
64.
Stefania Loreti Angela Gallelli Alessandra Belisario Eric Wajnberg Luciana Corazza 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(6):583-591
Xanthomonas arboricola pv. juglandis is the causal agent of walnut blight, one of the most important and widespread diseases of Persian (English) walnut (Juglans regiaL.), causing severe damage to leaves, twigs and nuts. To investigate the genomic variability of X. arboricolapv. juglandis, 66 isolates obtained from different countries (England, France, Italy, The Netherlands, Romania, Spain, USA, and New Zealand) were analysed using the Amplified Fragment Length Polymorphism (AFLP) technique. EcoRI and MseI were used as restriction endonucleases. Primers with a core sequence including endonuclease recognition sites and a 3prime-terminal cytosine selective base for MseI primer, or no selective base for EcoRI primer, were used. Data were analysed by means of a multiple correspondence analysis. A total of 76 amplified polymorphic DNA fragments were used to compute relationships among isolates. The AFLP profiles of X. arboricola pv. juglandis isolates appeared to be reliably distinguishable from X. arboricola pv. pruni and X. arboricola pv. corylina, and from other Xanthomonas species, i.e. X. campestris pv. campestris, X. fragariae, X. hortorum,
X. axonopodis pv. vesicatoria. Though this pathogen is associated with one single host genus, a high level of genomic diversity was found. This diversity might be partly explained by the geographic origin. Nevertheless, isolates with different patterns were collected within one country, and similar molecular patterns were found in isolates collected at different sites. However, genetic diversity might be influenced by exchanging vegetative material from different countries. Mixing of X. arboricola pv. juglandis isolates might have partly concealed the influence of the geographic location from which the bacteria were isolated. 相似文献
65.
Francesco Faretra Stefania Pollastro Francisco Sansiviero 《Pest management science》1992,35(2):171-174
‘Aliette’, a formulation of the fungicide fosetyl-aluminium, is moderately active in vitro against the ascomycete Botryotinia fuckeliana (Botrytis cinerea). In contrast, fosetyl-aluminium and related chemicals, such as phosphonic acid and sodium phosphonate, have low efficacy, although it is enhanced by media with low phosphate content. This indicates that the effectiveness of ‘Aliette’ against B. fuckeliana is not due to fosetyl-aluminium but to adjuvants included in the formulation. The genetic basis of differences in sensitivity to ‘Aliette’ was investigated in field and laboratory isolates of B. fuckeliana. Genetic analysis showed that reduced sensitivity to ‘Aliette’ always co-segregated with resistance to benzimidazole fungicides in meiotic progeny of sexual crosses. This indicates that differences in sensitivity to ‘Aliette’ in B. fuckeliana are due to the major gene Mbcl of resistance to benzimidazole fungicides and active adjuvant(s) present in ‘Aliette’ are linked to benzimidazoles by cross-resistance. 相似文献
66.
Stefania Pollastro Franco Faretra Vincenzo Di Canio Antonietta De Guido 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(7):607-613
Field isolates ofBotryotinia fuckeliana were collected from naturally infected plants. Their responses to the multisite fungicide dichlofluanid in mycelium growth test fell into three phenotypic classes, characterized by the following EC50 (and MIC) values ing ml–1: sensitivity, 1–3 (6–10); low resistance, 3–10 (> 100); high resistance, 10–30 (> 100). The corresponding values obtained for these classes in a spore germination test were respectively: 0.05 (0.2), 0.05–0.1 (0.5), 0.5–1 (0.9–1.5). Resistant isolates were crossed with two sensitive and two resistant strains of appropriate mating type to determine the genetic basis of resistance. Distribution of resistance phenotypes in ascospore progeny indicated that a gene, namedDic1, was probably responsible for the low or high resistance of 14 mutants selectively collected from experimental plots of greenhouse-grown gerbera sprayed several times with dichlofluanid or tolyfluanid. A second gene, namedDic2, was probably responsible for the low resistance displayed by two isolates (from grapevine and from carnation) maintained in the laboratory collection. As a result of the investigation, the use of dichlofluanid in integrated management programmes against grey mould is discussed. 相似文献
67.
Ilaria Biasato Manuela Renna Francesco Gai Sihem Dabbou Marco Meneguz Giovanni Perona Silvia Martinez Ana Cristina Barroeta Lajusticia Stefania Bergagna Luca Sardi Maria Teresa Capucchio Enrico Bressan Andrea Dama Achille Schiavone Laura Gasco 《畜牧与生物技术杂志(英文版)》2019,(3)
Background: The aim of this trial was to investigate the effects of different inclusion levels of a partial y defatted black soldier fly(BSF, Hermetia il ucens L.) larva meal on the growth performance, nutrient digestibility, blood profile, gut morphology and histological features of piglets. A total of 48 newly weaned piglets were individual y weighed(initial body weight(IBW): 6.1 ± 0.16 kg) and randomly al ocated to 3 dietary treatments(4 boxes as replicates/treatment and 4 animals/box). BSF larva meal was included at increasing levels(0% [BSF0], 5% [BSF5] and 10% [BSF10]) in isonitrogenous and isoenergetic diets formulated for two feeding phases: I(from d 1 to d 23) and I(from d 24 to d 61). The weight gain(WG), average daily gain(ADG), average daily feed intake(ADFI) and feed conversion ratio(FCR) were calculated for each feeding phase and for the whole trial. The haematochemical parameters and nutrient digestibility of the piglets were also evaluated. A total of 3 piglets per box were slaughtered on d 61 and the slaughtered piglets were submitted to morphometric investigations and histopathological examinations.Results: No overal significant differences were observed for growth performance(P 0.05), except for the ADFI of phase I, which showed a linear response to increasing BSF meal levels(P 0.05, maximum for the BSF10 group). Dietary BSF meal inclusion did not significantly influence the blood profile, except as far as monocytes and neutrophils are concerned,and these showed a linear and quadratic response, respectively, to increasing BSF meal levels(P 0.05, maximum for the BSF10 and BSF5 groups, respectively). On the other hand, the nutrient digestibility, gut morphology and histological features were not affected by dietary BSF meal inclusion(P 0.05).Conclusions: The obtained results show that a partial y defatted BSF larva meal can be used as a feed ingredient in diets for weaned piglets without negatively affecting their growth performance, nutrient digestibility, blood profile, gut morphology or histological features. 相似文献
68.
Laurence Vaissayre Morgane Ardisson Christiane Borries Sylvain Santoni Jacques David Pierre Roumet 《Euphytica》2012,185(1):61-75
Genetic maps published for elite durum wheat (Triticum turgidum durum Desf.), were mainly related to Italian and North American pools. To provide additional information dedicated to durum elite pool,
a recombinant inbred population (217 RILs) derived form 2 French commercial varieties (Ixos and Primadur), has been mapped
with 529 loci (98 AFLPs, 67 SSRs, 1 ISBP and 363 DArTs). Genetic map spanned a total of 2,082 cM (5.7 cM/marker on average).
Recombination rate variation over the genome was documented through the analysis of six segregating populations representing
a total of 48 RILs × 6, issued from an half diallel design based on four elite lines—Neodur, Ixos, Lloyd and Primadur—including
the 2 previous genitors. Each set of three genetically connected populations (48 RILs × 3) was used to build the consensus
parental maps for each genitor. To construct the four consensus parental maps, marker ordering was determined on 217 RILs
map which was an extended population of the most polymorphic cross (Ixos × Primadur). Sizeable, recombination rate variation
has been observed between the four parental maps both at the marker pair scale and at the linkage group scale. Two of the
parents, Lloyd and Primadur, had strong, opposite effects increasing and decreasing, respectively the recombination rate.
Additional studies are proposed to increase our understanding of this variation, with the identification of environmental
or genetic factors affecting recombination rate. 相似文献
69.
Cristian Mugnai Rosa M. Bertolotto Federico Gaino Christian Tiberiade Luca G. Bellucci Silvia Giuliani Stefania Romano Mauro Frignani Sonia Albertazzi Davide Galazzo 《Water, air, and soil pollution》2010,211(1-4):69-77
Due to its remarkable sediment pollution caused by past industrial activities, the Mediterranean coastal area facing the town of Cogoleto (near Genoa) has been declared of “national interest”. Seven sediment cores were analysed to provide information on history and trend of heavy metal inputs, with a particular focus on Cr. Grain-size compositions and 210Pb chronologies account for a strong influence of terrigenous inputs near the coast and the mouth of the Lerone Creek (draining the industrial settlement), with sand contents and accumulation rates varying from 40.3% and 0.7 cm year?1 near shore to 2.5% and 0.1 cm year?1 offshore. Heavy metal concentrations are high, notably Cr and Ni, and to a lesser extent, Ag, Hg, Pb, Cu and Zn. Cr, that shows past values up to 3,642 µg g?1, presents only minor recent decreases. In the area, it has both natural and anthropogenic sources and their relative contributions were distinguished on the basis of Cr/Ni ratios. These are 1–1.5 in soils and rocks of the Lerone Creek catchment area but reach very high values (up to 10) in marine sediments due to the anthropogenic contribution. The anthropogenic influence is higher near the creek mouth, gradually decreases offshore and is negligible at the most distal site (ca. 2.9 km from the coast). 相似文献
70.
Eleonora Bonifacio Gloria Falsone Stefania Piazza 《Journal of Soils and Sediments》2010,10(8):1475-1486