萧山草三黄鸡屠宰性能和肉质分析

研究对70日龄萧山草三黄鸡进行了屠宰性能和肉质性状的测定,结果显示:公鸡活重、屠体重、半净膛率和全净膛率优于母鸡,差异显著(P<0.05)。腿肌的肉色OD值、剪切力和pH值均大于胸肌,系水力小于胸肌,差异均显著(P<0.05);水分含量、粗脂肪含量大于胸肌,粗蛋白含量、粗灰分含量小于胸肌,差异均显著(P<0.05)。研究表明母鸡的肉质优于公鸡,屠宰性能则不及公鸡。     

苍耳等药用植物提取物对小菜蛾的拒食作用和产卵忌避效果

室内采用叶碟法,研究苍耳(Xanthium sibiricumPetril)等8种药用植物乙醇提取物对小菜蛾(Plutel-la xylostella)的拒食和产卵忌避活性。结果表明,苍耳(果实)、雷公藤(根)和商陆(根)乙醇提取物对小菜蛾4龄幼虫的取食均有较强的抑制作用,选择性拒食率分别为86.16%、49.06%和43.82%;非选择性拒食效果仍以苍耳(果实)提取物最好,达45.23%。选择性产卵忌避率以雷公藤(根)提取物为最高(69.52%),其次是苍耳(果实)和山苍树(枝叶)提取物,分别为62.50%和57.72%,雷公藤(根)、商陆(根)、窃衣(茎叶)和苍耳(果实)的非选择性产卵忌避作用均在77%以上。苍耳和雷公藤提取物在对小菜蛾的拒食和产卵忌避的各测试中,均表现较强活性。     

套袋对苹果生产投资结构的影响及密植园遮光问题

 套袋技术目前已经广泛应用于果树生产, 特别是富士苹果套袋率在山东和陕西主产区超过75% , 每亩套袋量8 000～18 000个, 纸袋投资270～1 100元, 占生产总投入比重的1 /3, 占生产资料平均投入的45%; 而套袋和摘袋雇工成本在人工成本中占主要部分, 套袋对苹果生产投资结构产生了巨大影响。对乔砧密植园大量套袋苹果树进行光合测定发现, 套袋造成了投影部位5片以上叶子遮光, 无论树冠外围还是内膛, 被遮光的叶片净光合速率均为负值, 大量套袋极显著降低了树冠中部和内膛照光叶片的光合能力。有关果实套袋技术的应用应该根据市场和生产条件细分确定。     

Relationship between uPA and NF-κB p65 expression and its clin ical significance in breast cancer

AIM:To evaluate the relationship between u PA and NF-κB p65 expression and its clinical significance in breast cancer.METHODS:The uPA mRNA was measured by real-time fluorescent quan t itative PCR in 46 cases of breast cancer tissues and their adjacent counterparts .NF-κB p65 were measured using immunohistochemistry.RESULTS:The expression of uPA gene was elevated in 63% of cases ,and there was a strong correlation between NF-κB p65 and uPA expression (r ＝0.451，P<0.01).In addition,the expression of uPA was associated with num bers of axillary lymph node involvement and tumor size.However,no significance was found in uPA expression in the age,pathological type and distant metastasi s organ classification.CONCLUSION:The uPA gene is highly expressed in most of the brea st cancer,and there is strong correlation to NF-κB p65 expression,number of l ymph nodes involvement and tumor size.     

合生素对肉鸭生产性能及胴体品质的影响

选用1日龄健康樱桃谷肉鸭新生雏144只，按体质量随机分为3组，每组设3个重复，每重复16只。对照组饲喂基础日粮，抗生素组添加5mg／kg黄霉素基础日粮，试验组1和组2在基础日粮中分别添加0．05％和0．10％合生素。结果表明，日粮中添加合生素对肉鸭日增质量、胸肌率和腿肌率有显著影响；对肉鸭的饲料转化率有极显著影响；对肉鸭的成活率、屠宰率和皮脂率无显著影响。与抗生素组相比，具有相似的作用。试验组1的日增质量比对照组提高8．56％，比试验组2提高5．54％，比抗生素组提高4．34％；试验组1的料肉比与对照组降低11．52％，比试验组2降低3．29％，比抗生素组降低3．24％。     

低温胁迫对东方山羊豆幼苗生理特性的影响

以东方山羊豆及敖汉苜蓿为材料,研究低温胁迫对东方山羊豆和敖汉苜蓿可溶性蛋白含量、保护酶活性、渗透调节物质的影响.结果表明:在低温胁迫下,东方山羊豆及敖汉苜蓿脯氨酸含量、SOD、POD活性、可溶性蛋白含量先上升后下降.经对几项生理指标的综合分析,认为东方山羊豆比敖汉苜蓿具较强的抗低温能力.     

Cloning of DNA fragments specific to the pathotype and race of <Emphasis Type="Italic">Verticillium dahliae</Emphasis>

Japanese isolates of Verticillium dahliae, a causal agent of wilt disease in many plants, are classifiable into pathotypes based on their pathogenicity. Because these pathotypes are morphologically indistinguishable, establishing a rapid identification method is very important for the control of this pathogen in Japan. For cloning DNA fragments that are useful for identification and specific detection of V. dahliae pathotypes, we performed random amplified polymorphic DNA (RAPD) analyses using various isolates. One polymerase chain reaction (PCR) product, E10-U48, was specific to isolates pathogenic to sweet pepper. The other product, B68-TV, was specific to race 1 of isolates pathogenic to tomato. The specificity of these sequences was confirmed by genomic Southern hybridization. Further analyses revealed that the region peripheral to B68-TV obtained from the genomic DNA library includes the sequence specific to all isolates pathogenic to tomato (races 1 and 2). Moreover, sequence tagged site (STS) primers designed from B68-TV and its peripheral region showed race-specific and pathotype-specific amplification in a PCR assay. The probes and primers obtained in this study are likely to be useful tools for the identification and specific detection of pathotypes and races of V. dahliae. The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB095266.     

应用银黑狐精液改良蓝狐配种技术

试验结果表明 ,利用银狐精液 ,采用人工授精的方法改良蓝狐得到很好的效果 ,受胎率可达 92 %。     

聚合物发光二极管的研究进展

对共轭聚合物中进行电运输及电致发光器件－聚合物发光二极管的研究进展进行了综述。     

牛蛙腐皮病病变皮肤亚显微结构的观察

为揭示牛蛙腐皮病的病理形态和发病的机理，采用透射电镜对病蛙不同病期的病变皮肤进行了观察，皮肤病变早期可见角化层脱落，状上皮细胞连接疏松，桥粒减少，随着病情发展细胞间隙增宽，桥粒进一步减少，细胞核固缩、溶解，细胞裂解；病变后期，表皮层崩解，真皮层胶原纤维溶解，炎症区有大量炎症细胞浸润，并可见吞噬了细菌的单核与中性粒细胞。