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排序方式: 共有116条查询结果,搜索用时 15 毫秒
91.
M Shirai H Izumi T Yamagami 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(4):707-713
Two new experimental models of transplantable mouse sarcoma 180 were developed in ICR mice in order to examine the optimum transplantation sites and methods. The cervicodorsal hypoderm was evaluated as the best transplantation site for mouse sarcoma 180 among seemingly usable transplantation sites such as groin, armpit, cervicodorsal, abdominal and lumbodorsal hypoderms by hypodermic transplantation. In addition, the lung transplantation model was established by monitoring the survival period as a reliable parameter for evaluation of anti-tumor effects. 相似文献
92.
The virus distribution and histopathologic changes in organs of 1-week-old chickens inoculated with three representative isolates of Newcastle disease virus isolated from racing pigeons in Japan were examined. All three isolates were recovered from various organs, including brain, for several days, but not from the blood. Results were highly correlated with their high intracerebral pathogenicity indices (ICPI), in spite of their long mean death time of minimum lethal dose (MDT/MLD). 相似文献
93.
94.
Nakao K Sato T Shirai W Matsuo K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(2):191-193
A malignant basal cell tumor was found in the skin of the abdomen of a female Djungarian hamster of unknown age and weighing 40 g. Histologically, the tumor mass was composed of cells resembling the basal cells of the epidermis, and these cells exhibited solid type proliferation. In the tumor tissue and necrotic foci, horn cysts were observed. Immunohistochemically, cytokeratin was present in the tumor cells and horncysts. By electron microscopic examination, the tumor cells had scanty cell organelles and a few desmozomes. This paper describes a rare malignant basal cell tumor in a Djungarian hamster. 相似文献
95.
Shugo Suzuki Aya Naiki-Ito Toshiya Kuno Wanisa Punfa Ne Long Hiroyuki Kato Shingo Inaguma Masami Komiya Tomoyuki Shirai Satoru Takahashi 《Journal of toxicologic pathology》2015,28(1):21-26
We previously established 3 cell lines (PLS10, PLS20 and PLS30) from a chemically-induced prostate carcinoma in F344 rats, and demonstrated high potential for metastasis in nude mice. In the present study, we investigated the feasibility of establishing an orthotopic model using the 3 rat prostate cancer cell lines in immunocompetent rats with the aim of resolving species-mismatch problems and defects of immune systems. The PLS10, PLS20 and PLS30 cell lines were injected into the ventral prostates of 6-week-old rats, which were then sacrificed at experimental weeks 4 and 8. Tumor mass formation was found in rats with PLS10, but not in those with PLS20 or PLS30. Additionally, metastatic carcinomas could be detected in lymph nodes and lungs of PLS10-inoculated rats. Genetic analysis demonstrated K-ras gene mutations in PLS10 and PLS20, but not in PLS30 cells. There were no mutations in p53 and KLF6. In conclusion, we established a syngeneic orthotopic model for prostate cancer in immunocompetent rats simulating human castration-resistant prostate cancer (CRPC), which should prove useful for development and validation of therapeutic agents, especially with immunotherapy. 相似文献
96.
Ricardo P Babaran Kazuhiko Anraku Munechika Ishizaki Kenji Watanabe Tatsuro Matsuoka Hideaki Shirai 《Fisheries Science》2008,74(6):1207-1214
Sound generated by a payao, an anchored bamboo fish aggregating device, is believed to be attractive to fish; but until now,
there is no available record of payao-generated sound. This study presents payao-generated sound recorded by a hydrophone
at water depths of 5, 10 and 15 m from a fixed distance of 3 m relative to the payao, and compares the sound with the auditory
sensitivity of jack mackerel Trachurus japonicus measured at discrete frequencies from 100 to 2000 Hz using the auditory brainstem response protocol. A consistent peak appeared
in the sound spectrum at 49 Hz and showed an increasing sound pressure level with depth, which suggests that payao sound may
come from the anchor rope. However, the contribution of the bamboo raft can not yet be discounted. The hearing threshold curve
indicated that the most sensitive frequency range in jack mackerel is from 92.1 dB at 800 Hz to 111.0 dB at 200 Hz. These
results show that the dominant frequency range of payao sound does not correspond with the high sensitivity frequency range
of fish hearing. 相似文献
97.
Gimeno M Ramírez-Hernández JY Mártinez-Ibarra C Pacheco N García-Arrazola R Bárzana E Shirai K 《Journal of agricultural and food chemistry》2007,55(25):10345-10350
Free astaxanthin one-solvent extractions with ethanol, acetone, and liquid 1,1,1,2-tetrafluoroethane from raw and lactic acid fermented (ensilaged) shrimp residues were investigated. The total carotenoid recovery from ensilaged shrimp wastes was higher than that from non-ensilaged ones as assessed by HPLC analyses. Acetone gave the highest extraction yields of free astaxanthin with up to 115 microg/g of material. Moreover, liquid tetrafluoroethane is reported for the first time in a successful one-solvent extraction of carotenoids from shrimp. 相似文献
98.
Yamaguchi T Kanemitsu H Yamamoto S Komatsu M Uemura H Tamura K Shirai T 《Journal of toxicologic pathology》2010,23(1):25-30
N,N'-bis(2-chloroethyl)-N-nitrosourea (BCNU) is one of the major drugs used in chemotherapy against malignant gliomas due to its effects, such as induction of bifunctional alkylation of DNA and formation of interstrand DNA cross-linkages, and induces cortical malformations in the fetal and neonatal rat brain. In this study, pregnant rats were treated with 7.5 mg/kg of BCNU on gestational day 13 (GD 13), and their fetuses were collected from 12 to 72 hours after BCNU treatment in order to examine the timecourses of morphological and immunohistochemical changes in neural progenitor cells in the developing brain. The number of pyknotic cells in the telencephalon peaked at 24 h and then gradually decreased until 72 h. The majority of these pyknotic cells were positive for cleaved caspase-3, a key executioner of apoptosis. The pyknotic cells showed the ultrastructural characteristics of apoptosis. The number of p53-positive cells began to increase prior to the appearance of apoptotic cells and p21-positive cells. The number of phosphorylated-histone H3-positive cells (mitotic cells) decreased from 24 to 36 h. The number of Iba1-positive cells (microglial cells) in the telencephalon increased from 12 to 48 h. These results suggest that BCNU induces p53-dependent apoptosis and reduces proliferative activity, resulting in reduction of the weight of the telencephalon and the thickness of the telencephalic wall in the fetal brain. This study will help to clarify the mechanisms of BCNU-induced fetal brain toxicity. 相似文献
99.
The effects of streptozotocin (STZ)-induced diabetes on induction of hepatic preneoplastic lesions by diethylnitrosamine (DEN) were investigated in male Fischer rats. A single dose of STZ was injected intravenously either 2 weeks before or after initiation with DEN. The blood glucose levels were significantly elevated from 1 week after STZ-injection until autopsy. The numbers of GST-P positive foci at 1 week after DEN administration in the STZ-injected rats were similar to those in the non-diabetic rats. In contrast, both the numbers and areas of GST-P positive foci > 2 mm in diameter 8 weeks after DEN administration were increased significantly in the rats treated with STZ after DEN exposure compared with the non-diabetic control rats. The results suggest that hepatic preneoplastic lesions initiated with DEN are promoted by STZ treatment-inducing diabetes. 相似文献
100.
The present study examined whether the elemental compositions of whole otoliths and chronological transect profiles of otoliths
covering the complete life history are useful for evaluating the population structure of Pacific herring. Eight elemental
ratios, Li:Ca, Na:Ca, Mg:Ca, K:Ca, Mn:Ca, Cu:Ca, Sr:Ca, and Ba:Ca, were measured in whole otoliths and in a series of ablations
across the life-history transects by laser ablation inductively coupled plasma mass spectrometry. We studied fish from three
sample groups collected from Lake Obuchinuma, Miyako Bay, and Akkeshi Bay of northern Japan from March to May 2008. Significant
differences were found in the Na:Ca, Mg:Ca, P:Ca, K:Ca, Mn:Ca, and Ba:Ca ratios of the whole otoliths and in the Li:Ca, Na:Ca,
K:Ca, Mn:Ca, Sr:Ca, and Ba:Ca ratios of the otolith core among the three sample groups. Therefore, the present study showed
clear differences in elemental composition among the three sample groups from different locations, suggesting that otolith
elemental compositions reflect the habitats experienced by individual fish. 相似文献