Association of a Geminivirus with a Leaf Curl Disease of Sunn Hemp (Crotalaria juncea) in India

Natural occurrence of a geminivirus causing severe leaf curl disease on sunn hemp (Crotalaria juncea) was recorded in India. The association of a geminivirus with the disease was demonstrated by whitefly transmission tests and polymerase chain reaction (PCR) amplification of DNA fragments of expected sizes with three pairs of degenerate geminivirus primers. The PCR-amplified viral DNA fragments were further characterized by Southern hybridization with a geminivirus probe consisting of the cloned coat protein (CP) gene of Indian tomato leaf curl virus (ITLCV). Restriction fragment length polymorphism analysis of a PCR-amplified CP fragment revealed that the geminivirus from sunn hemp was different than ITLCV.     

Changes in vasotocin immunoreactivity of paraventricular nuclei and adrenal function of Japanese quail in relation to different phases of sexual development

In relevance to osmoregulatory and reproductive functions, activity of hypothalamic neurosecretory neurons may also vary seasonally. The current study was performed to determine annual changes in ir-AVT neurons of hypothalamus and adrenal gland function. We examined changes in ir-AVT neuron by immunohistochemical method and plasma testosterone was measured by enzyme immunoassay. The steroidogenic interrenal activity was studied by histological and biochemical methods. Birds were sampled in February (quiescent), April (recrudescent), June (breeding) and November (regressive). A significant and gradual increase in the number of ir-AVT neurons was observed from quiescent to breeding phase which decreased during regressive phase of annual gonadal cycle. The gradual increase in ir-AVT neurons along with annual gonadal activity of quail were accompanied by increase in plasma levels of testosterone. These results indicate a functional interaction between sex steroid and AVT synthesizing neurons. Adrenal activity (as judged by weight, ascorbic acid content, cortical cord width and cortico-medullary ratio) was also maximum during breeding phase. It is thus postulated that domesticated quail when exposed to natural day length (NDL), exhibits seasonal/annual cyclicity in vastocinergic activity and adrenal function which may be due to difference in sex steroid hormone.     

Comparison of Composition,Protein, Pasting,and Phenolic Compounds of Brown Rice and Germinated Brown Rice from Different Cultivars

Physical characteristics, amino acids composition, protein profiling, pasting characteristics, and phenolic compounds of brown rice (BR) and germinated brown rice (GBR) from different paddy cultivars (PB1, PS44, PB1509, PB1121, and PS5) were investigated. L* (lightness) decreased, but a* (redness and greenness) and b* (yellowness and blueness) increased with germination. Protein and ash content increased, whereas fat and amylose contents decreased with germination. GBR showed lower hardness and gumminess than BR. Foam stability and water absorption capacity from GBR flour were higher compared with BR flour. Accumulation of γ‐aminobutyric acid, histidine, arginine, proline, methionine, and acidic amino acids increased significantly with germination, and increase was related to change in accumulation of glutelin and prolamins. The accumulation of prolamins and glutelin acidic and basic subunits decreased with germination. GBR flour showed lower pasting viscosities compared with BR flour. Ferulic acid, p‐coumaric acid, and quercetin were present in both fractions of the bound form. GBR showed improved nutritional quality that varied in different cultivars. PB1121 was observed to be the best for producing GBR owing to greater changes brought in protein content, essential amino acids, catechin, chlorogenic acid, protocatechuic acid, vanillic acid, and foam stability.     

Soil Organic Carbon and Soil Physical Characteristics as Affected by Land Uses under Semiarid Irrigated Conditions

ABSTRACT

Rice-Wheat rotation is the dominant land use in the state of Punjab, resulting in over exploitation of ground water resources. Thus, it is necessary to evaluate other land uses that requires less water and are sustainable. The present investigation was planned with four land uses viz., fallow (FLU), rice-wheat (ALU), grasses (GLU) and pear (PLU) with respect to their effect on soil organic carbon and soil physical characteristics in surface and subsurface depth in district Ludhiana, Punjab. The soil organic carbon (SOC) content was higher by 10, 30.9 and 24.9% under rice-wheat, grasses, and pear than that under fallow. The grasses showed higher soil moisture characteristics curve (SMCC) and lower bulk density (Db) than that under rice-wheat. The larger mean weight diameter (MWD) were observed under pear, grasses and fallow than that under rice-wheat by 0.21, 0.51 and 0.41 mm, respectively. The saturated hydraulic conductivity (K_s) was higher in magnitude by 56.1, 55.4 and 28%, respectively under PLU, GLU and FLU over ALU. Of the evaluated land uses, pear and grasses proved to be more sustainable by retaining more moisture, maintaining better soil physical characteristics and SOC under semiarid irrigated conditions in the state on long term gradual response.     

Status of floor vegetation under some monoculture and mixculture plantations in North India

A study conducted to assess the floor vegetation shows the presence of 17 species underPopulus deltoides in comparison to 28, 29 and 35 underAlbizia lebbeck, Dalbergia sissoo and mix-culture plantations, respectively. Further, the biomass and density of floor vegetation is considerably reduced underP. deltoides compared to other plantations. In addition, the indices for species richness, evenness, dominance and diversity are also reduced underP. deltoides showing some inhibitory mechanism being operative under these plantations. Based on available literature, allelopathy is speculated to be responsible for this reduction.     

Measured and predicted transport of two S-triazine herbicides through soil columns

Effluent concentration of chloride and two pesticides (prometon and atrazine) were measured during column displacement experiments at two water flow rates. A constant suction of approximately 1300 Pa was maintained in the packed soil columns which were positioned vertically on top of a vacuum chamber enclosing an automatic fraction collector. Measured breakthrough curves (BTC's) were analyzed in terms of two solute transport models: the standard two-parameter convection-dispersion equation (CDE), and a four-parameter two-site/two-region nonequilibrium model (TRM). Calculations obtained with the TRM model were found to be in better agreement with measured BTC's than predictions using the CDE model. Column retardation factors for prometon and artrazine calculated from equilibrium batch sorption coefficients were comparable to those estimated from the observed BTC's only when the nonequilibrium TRM model was used.     

High yielding spring bread wheat germplasm for global irrigated and rainfed production systems

Global wheat (Triticum aestivum L.) production must increase 2% annually until 2020 to meet future demands. Breeding wheat cultivars with increased grain yield potential, enhanced water-use efficiency, heat tolerance, end-use quality, and durable resistance to important diseases and pests can contribute to meet at least half of the desired production increases. The remaining half must come through better agronomic and soil management practices and incentive policies. Analyses of the recent International Yield Trials indicate that grain yields of the best new entries were usually 10% higher than the local checks globally, as well as within a country across sites. Variation in yield across sites within a country/region underline the role of genotype × environment (GE) interaction and provides opportunities to select for stable genotypes, which is not often done. The lack of proper analysis undermines proper utilization of germplasm with high yield potential and stability in the national wheat breeding programs. Some of the best performers in irrigated areas were amongst the best in semiarid environments, reinforcing the fact that high yield potential and drought tolerance can be improved simultaneously. The best performing lines often had genotypic base of widely adapted genotypes Kauz, Attila, Baviacora, and Pastor, with genetic contributions from other parents including synthetic wheat. We recommend within country multilocation analysis of trial performance for a crop season to identify lines suiting particular or different locations within a country. The immediate feedback on GE interaction will also help in breeding lines for countries having substantial variation across locations and years.     

Crop productivity under differently lopped canopies of multipurpose trees in Central Himalaya, India

Ficus species are multipurpose trees well known by rural populations in Sahelian and Sudanian zones of Africa. Their uses are well documented but their amenability for vegetative propagation has not been extensively studied. This paper compares the rooting ability of stem and aerial root cuttings from thirteen Ficus species found in West Africa. It highlights the differences between species belonging to the sub-genera Sycomorus and Urostigma. The former show no capacity to propagate from cuttings whereas the latter, with epiphytic development, can be propagated by cuttings, although this capacity varies among species. Thus, F. thonningii, F. leprieurii and F. ovata are easily propagated, while F. platyphylla and F. elasticoides are propagated with difficulty. The rooting capacity also varies depending on the cutting material used. It decreases in the following order: long leafless hardwood cuttings (pole) > nodal cuttings > apical cuttings. Rooting potential increases when the cuttings are harvested towards the end of the dry season(March to May). Aerial root can be used for cuttings in all species of the sub-genus Urostigma. The capacity of root cuttings to regenerate is greatest when cuttings are collected at the beginning of the dry season (November). In this case, wound-induced adventitious roots arise at the basal end of the cutting while de novo buds are developed from the cambium at the distal end. The subsequent morphological development is identical to that of a stem cutting. These results clarify and allow the optimal use of the knowledge and methods developed by the indigenous people of the Sahel and could assist and promote fig tree (Ficus sp.) domestication in the dry tropics.This revised version was published online in November 2005 with corrections to the Cover Date.     

Influence of sources and doses of N and K on herbage,oil yield and nutrient uptake of patchouli [Pogostemon cablin (Blanco) Benth.] in semi-arid tropics

Patchouli oil is one of the most important essential oils used in modern perfumery and cosmetic industries. There is hardly any preparation of oriental nature where patchouli oil is not used. It is used mainly because of fixative property as it gives tenacity to other perfumes. Field experiments were conducted at Bangalore, India, to study the influence of sources and doses of N and K on herbage, oil yield, nutrient uptake, nitrogen utilization efficiency and oil content of patchouli [Pogostemon cablin (Blanco) Benth.]. The results revealed that application of 200 kg N/ha and 41.5 kg K/ha produced significant higher patchouli herbage and oil yields compared with controls. Similarly, N and K uptake were also higher at 200 kg N and 41.5 kg K/(ha year) compared with controls. DCD-coated urea produced higher herbage, oil yield and N uptake and utilization efficiency compared with prilled urea. There was no effect of sources of K on the yield of patchouli. The oil content was not influenced by N, K doses or sources applied. N and K depletion were noticed in the soil.     

Community forestry for revitalising rural ecosystems: A case study

A case study focusing on the socio-economic and ecological aspects of a forest-based village, Debrasai, in the Singhbhum District of Bihar, India was undertaken in 1980 to evolve village-specific community forestry package programmes that could bring stability to the village both economically as well as ecologically. The village extending over 365.07 ha is inhabited by 361 people comprising 195 males and 166 females in 81 households. The villagers are by and large dependent on agriculture and forestry for their livelihood. The largest proportion of the land in the village is under agriculture whereas forests, in a highly degraded state, occupy only 5.7% of the total area. The villagers exploit the protected forests in their vicinity removing nearly 350.9 mt of fuelwood, 22.8 mt of small timber and 393.4 mt of fodder annually.The village has a manpower potential of 40 035 man days a year. Agriculture is the main source of employment at the moment absorbing 14 266 man days. The non-agricultural sector provides employment to an extent of 4433 man days. Fresh employment opportunities are needed to absorb nearly 53% of man days of the village at present unused. Three land-resource, skill-based package programmes, a tasar silk culture, an energy plantation, and dairying have been suggested to generate employment opportunities and to meet the basic needs of the villagers. About 115 ha of the hitherto neglected land resource has been located in the village to sustain such community forestry (CF) programmes. Tasar culture suggested on 35 ha will alone generate employment to an extent of 18 690 man days from the fourth year of tasar silk plantation. A plantation, to provide energy and fodder, on 25 and 30 ha respectively is expected to yield fuelwood, timber and fodder to more than satisfy village needs. A dairy has been recommended as it will generate income, provide nutrition and promote gobar gas production — an alternative energy source to fuelwood. The cost—benefit analysis of these projects indicates their economic feasibility with the cost likely to be recovered in 4.5 years. Debrasai being an agricultural village, a land-inequity compensatory scheme has been suggested for CF project net benefit sharing, ensuring reasonable benefit flows to the landless and marginal farmers.