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331.
332.
Masoumeh Firouzamandi Hassan Moeini Davood Hosseini Mohd Hair Bejo Abdul Rahman Omar Parvaneh Mehrbod Aini Ideris 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):21-26
The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure. 相似文献
333.
Diaa Massoud Mohamed Abumandour Hamida Hamdi Kareem Morsy Attalla F. El-kott Amany Attaallah Fatma Mohsen Shalaby 《Anatomia, histologia, embryologia》2023,52(2):318-326
The present work was designed to investigate the microscopic structure of the oesophagus in the southern white-breasted hedgehog (Erinaceus concolor) using histochemical staining, Scanning electron microscope (SEM), and stereological procedures. Four adult males were included in our study. Serial sections of the entire length of the oesophagus were stained with aldehyde fuchsin, alcian blue (pH 2.5), Periodic acid Schiff (PAS), and Masson's trichrome. Then, the total volume of the oesophagus, and the total volume of its different layers, were estimated using Cavalieri's principle. The oesophageal epithelium was a non-keratinized stratified squamous type. Muscularis mucosa was present as a thick layer between lamina propria and submucosa and its thickness was increased toward the stomach. Tunica submucosa was a loose connective tissue containing an oesophageal gland with PAS-positive and AB-positive reactions throughout the submucosa and become denser toward the stomach. The tunica muscularis consisted of two distinct striated muscle layers, and its thickness was decreased toward the stomach. On SEM images, the cervical and thoracic oesophagus showed shallow folding, while the abdominal part had deeper folds. The present findings indicate that the histological properties of the oesophagus in southern white-breasted hedgehogs have slight similarities with rodents and considering its epithelium, submucosal glands and tunica muscularis more resemble with dog oesophagus. The obtained results may be quite helpful to improve the current knowledge of the histophysiology of the hedgehog gastrointestinal tract as a member of eulipotyplan mammals and as a pet for biologists and veterinarians. 相似文献
334.
Gagnon CA Tremblay D Tijssen P Venne MH Houde A Elahi SM 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(8):811-819
Since late 2004, the swine industry in the province of Quebec has experienced a significant increase in death rate related to postweaning multisystemic wasting syndrome (PMWS). To explain this phenomenon, 2 hypotheses were formulated: 1) the presence of a 2nd pathogen could be exacerbating the porcine circovirus 2 (PCV-2) infection, or 2) a new and more virulent PCV-2 strain could be infecting swine. In 2005, 13 PMWS cases were submitted to the Quebec provincial diagnostic laboratory and PCV-2 was the only virus that could be found consistently by PCR in all 13 samples. The PCR detection results obtained for other viruses revealed the following: 61.5% were positive for porcine reproductive and respiratory syndrome virus, 30.8% for swine influenza virus, 15.4% for porcine parvovirus, 69.2% for swine torque teno virus (swTTV), 38.5% for swine hepatitis E virus (swHEV) and 84.6% for Mycoplasma hyorhinis; transmissible gastroenteritis virus and porcine respiratory coronavirus (TGEV/PRCV) was not detected. Sequences of the entire genome revealed that these PCV-2 strains belonged to a genotype (named PCV-2b) that has never been reported in Canada. Further sequence analyses on 83 other Canadian PCV-2 positive cases submitted to the provincial diagnostic laboratory during years 2005 and 2006 showed that 79.5% of the viral sequences obtained clustered in the PCV-2b genotype. The appearance of the PCV-2b genotype in Canada may explain the death rate increase related to PMWS, but this relationship has to be confirmed. 相似文献
335.
336.
The current method to detect antibody titre against infectious bursal disease virus (IBDV) in chickens is based on enzyme-linked immunosorbent assay (ELISA) using whole virus as coating antigen. Coating the ELISA plates requires a purified or at least semi-purified preparation of virus as antigen, which needs special skills and techniques. In this study, instead of using whole virus, recombinant protein of hexahistidine tag (His 6 tag) and VPX protein of IBDV expressed in E. coli was used as an alternative antigen to coat the ELISA plates. There was a good correlation coefficient (R2 = 0.972) between the results of the ELISA using plates coated with monoclonal antibody against His 6 tag and those of the commercial IBDV ELISA kit. Hence, His 6 tag and VPX recombinant protein expressed in E. coli has the potential for the development of ELISA for the measurement of IBDV-specific antibody. 相似文献
337.
伞伐更新法对赫卡尼亚(Hyrcanian)森林系统中森林再生和林分结构的影响 总被引:1,自引:0,他引:1
Mohammad Reza Pourmajidian Hamid Jalilvand Asghar Fallah Seyed Ataollah Hosseini Aidin Parsakhoo Amin Vosoughian Ali Rahmani 《林业研究》2010,21(3):265-272
研究伞伐更新法对赫卡尼亚森林系统中森林再生和林分结构的影响。在伊朗北部优势种为山毛榉(Fagus orientalisLipsky)的硬木林,采取伞伐更新法和非伞伐更新法方法设计实验区。结果表明,在山毛榉(F orientalis Lipsky)林中采用伞伐更新法处理,明显影响林下草本植物种的频度和密度。伞伐更新法处理后,林堇菜(Viola silvestris Lam.)、车叶草(Asperula odorata L.)、苔草(Carex spp.)和悬钩子(Rubushyrcanus Juz)的种频度明显增加。在对照区,树种的胸高直径(57.50±2.15cm)大于被处理区(50.67±1.88cm)的树种的胸高直径(50.67±1.88 cm),但对照区和处理区的树种的高度值相似。在1995-2005年间,波斯铁木(Parrotia persica)苗木数量增加到13.2%,而山毛榉(F orientalis)和欧洲鹅耳枥(Carpinus betulus)的苗木数量却明显减少。总之,应该利用其它育林方法,如,带状择伐作业,而不是伞伐更新法培育赫卡尼亚森林中山毛榉林。 相似文献
338.
Mohsen Javanmiri Pour Mohammad Reza Marvi Mohadjer Vahid Etemad Meghdad Jourgholami 《林业研究》2019,(5):1859-1871
Numerous approaches have been developed to quantify forest structure modules.A variety has measured each as part of stand attributes.This study was carried out in managed mixed stands in northern Iran.The objective was to quantify stand structure and its variation before and after tree marking through the Gini index and structural triangle method.A full sampling inventory was taken in 2004 and 2014 at the beginning and end of a period,before and after tree marking operations in five stands.The results indicate that the Gini index was 39.5,62.2,43.0,82.0 and 74.0%forⅠ,Ⅱ,Ⅲ,ⅣandⅤstands respectively.Furthermore,approximate structural diameters were determined as a large category forⅠ,Ⅱ,ⅢandⅤstands while standⅡhad an intermediate-large class pre-tree marking to an intermediate-small class post-tree marking.The paired t-test results were not significant for tree numbers and growing stock changes for all species,and for beech,hornbeam,and velvet maple before and after tree harvesting in the whole stand.Therefore,the Gini index and the structural triangle method can be used for natural stand modeling,structural diversity designation,and for management practices in nature-oriented forestry strategies. 相似文献
339.
MA Shokrgozar M Fattahi S Bonakdar I Ragerdi Kashani M Majidi N Haghighipour V Bayati H Sanati SN Saeedi 《Iranian Biomedical Journal》2012,16(2):68-76
Background: Wound healing of burned skin remains a major goal in public health. Previous reports showed that the bone marrow stem cells were potent in keratinization and vascularization of full thickness skin wounds. Methods: In this study, mesenchymal stem cells were derived from rat adipose tissues and characterized by flowcytometry. Staining methods were used to evaluate their differentiation ability. A collagen-chitosan scaffold was prepared by freeze-drying method and crosslinked by carbodiimide-based crosslinker. Results: The results of immunecytochemistry and PCR experiments confirmed the adipose-derived stem cells (ASC) in differentiation to the keratinocytes under the treatment of keratinocyte growth factor. The isolated ASC were seeded on the scaffolds and implanted at the prepared wounds. The scaffolds without cells were considered as a control and implanted on the other side of the rat. Histopathological analyses confirmed the formation of new tissue on the scaffold-cell side after 14 days with the formation of dermis and epidermis. Conclusion: These results indicated the capacity of ASC in differentiation to keratinocytes and also wound healing in vivo. Key Words: Tissue engineering, Keratinocytes, Mesenchymal stem cells 相似文献
340.
Mosayeb Rostamian Seyed Jafar Mousavy Firouz Ebrahimi Seyyed Abolghasem Ghadami Nader Sheibani Mohammad Ebrahim Minaei Mohammad Ali Arefpour Torabi 《Iranian Biomedical Journal》2012,16(4):185-192