Indirect Organogenesis through Seedling-Derived Leaf Segments of <Emphasis Type="Italic">Ficus Religiosa</Emphasis> - a Multipurpose Woody Medicinal Plant

The aim of this study is to introduce the suitable protocol for indirect regeneration from seedling-derived leaf segment of Ficus religiosa. The leaf explant successfully produced callus on MS medium containing various concentrations of auxin in combination with BAP. The maximum callus induction (100%) was achieved in MS medium containing 0.5 mg/l 2,4-D plus 0.05 mg/l BAP and MS medium containing 1.5 mg/l NAA plus 0.15 mg/l BAP as well. MS medium consisting of 2,4-D produced yellow-brownish and friable callus (type I) while the yellowish and compact calli (type II) were obtained in MS medium consisting of NAA. On the other hand, MS medium supplemented with IBA formed greenish and compact calli (type Ш). The regeneration rate in type II callus was less than the type I, and there was no shoot induction observed on type Ш calli. MS medium supplemented with 1.5 mg/l BAP in combination with 0.15 mg/l IBA had the highest regeneration frequency (100%) and maximum shoot numbers (5.16) as well as shoot length (2.56 cm) in type I callus. A maximum of 93.33% root induction was observed in MS medium supplemented with 2.0 mg/l IBA plus 0.1mg/l NAA. The plantlets were successfully transferred to the greenhouse. This system could be utilized for large-scale multiplication of Ficus religiosa.     

An evaluation of harvesting costs in present and planned operations: case study—Waston District of Mazandaran Wood and Paper Industries

Amongst all forest utilization phases, harvesting plays a key role as the beginning phase of the utilization cycle and has a significant effect on the following phases.In this research, in order to examine the reduction of costs with respect to present and planned operations, the starting time of the harvesting or cutting phase and work flow were recorded. The starting time of each phase of the entire operation was recorded as well. Before harvesting the marked tree, the appropriate felling direction was determined and the azimuth of the correct direction was taken.The results indicate that there was not a meaningful relationship between the differences in direction of a leaning tree compared with the felling direction. However, there was a significant relationship between the difference of the defined and felling direction and the difference in tree leaning and defined direction. The same trend was observed between tree leaning and felling directions and tree leaning and defined directions. On the other hand,there was no significant relationship between tree leaning and felling directions and the defined and tree felling directions. In addition, this research shows that with an increase in tree volume, the time for tree cutting rose.Furthermore, when the difference of tree leaning and felling direction decreased, the time to cut the tree declined.The regression equation is Y = 168.9–0.14(the difference between tree leaning and felling direction) + 0.7(volume).     

Modelling the spatial distribution of snake species in northwestern Tunisia using maximum entropy(Maxent) and Geographic Information System(GIS)

We used GIS and maximum entropy to predict the potential distribution of six snake species belong to three families in Kroumiria(Northwestern Tunisia): Natricidae(Natrix maura and Natrix astreptophora), Colubridae(Hemorrhois hippocrepis, Coronella girondica and Macroprotodon mauritanicus), and Lamprophiidae(Malpolon insignitus). The suitable habitat for each species was modelled using the maximum entropy algorithm, combining presence field data(collected during 16 years:2000–2015) with a set of seven environmental variables(mean annual precipitation, elevation, slope gradient,aspect, distance to watercourses, land surface temperature and normalized Differential Vegetation Index. The relative importance of these environmental variables was evaluated by jackknife tests and the predictive power of our models was assessed using the area under the receiver operating characteristic. The main explicative variables of the species distribution were distance from streams and elevation, with contributions ranging from 60 to 77 and from 10 to 25%,respectively. Our study provided the first habitat suitability models for snakes in Kroumiria and this information can be used by conservation biologists and land managers concerned with preserving snakes in Kroumiria.     

Differential Response to Salt Stress Within and Among Iranian Melilotus Species

This paper explores the salt-tolerance response of Melilotus species, a forage legume, based on tolerance/susceptibility indices, leaf water relations, leaf/root ion concentrations and multivariate (factor and function discriminate) analyses. In a greenhouse experiment, salinity-susceptibility of 12 accessions of Melilotus (M. albus, M. indicus, M. officinalis) in addition, two controls (alfalfa and Persian clover) were evaluated at 0 mM, 100 mM, 200 mM, and 300 mM of sodium chloride (NaCl). The experiment was conducted in a split plot design with two replications. Stress tolerance score (STS) was calculated based on tolerance/susceptibility indices. Salt-tolerant accessions were identified. An extracted bi-plot based on factor analysis confirmed the results of tolerance/susceptibility indices. There were significant correlations between STS and multivariate statistics. Root potassium cation (K⁺) concentration was higher in Melilotus species than the control species in all the salinity treatments. STS, root K⁺ concentration, leaf turgidity, and leaf succulence characteristics can be used for screening salinity-tolerance in Melilotus accessions. In all treatments, M. officinalis, M. indicus, and M. albus were salinity-tolerant species in descending order.

Abbreviations: Total biomass yield under control (Yp) and stress (Ys) mediums, geometric mean productivity (GMP), leaf area (LA), leaf tissue density (LTD), linear regression coefficient (b), mean productivity (MP), relative water content (RWC%), Succulence (S), specific leaf area (SLA), stress susceptibility index (SSI), stress tolerance index (STI), tolerance (TOL), water content at saturation (WCS), water saturation deficit (WSD), sodium chloride (NaCl), potassium cation (K⁺), sodium cation (Na⁺), analysis of variance (ANOVA), least significant difference (LSD), alfalfa control accession (CHA), Persian clover control accession (CHC), coefficient of variation (CV), statistics of factor (F) and discriminate function (D)     

Natural Dyeing of Wool by Madder (<Emphasis Type="Italic">Rubia tinctorum L</Emphasis>.) Root Extract Using Tannin-based Biomordants: Colorimetric,Fastness and Tensile Assay

In this work, the natural dyeing behavior of woollen yarn with madder (Rubia tinctorum L.) root extract was studied. The effects of different tannin-rich plants (Rhus coriaria, Eucalyptus, Terminalia chebula, Quercus castaneifolia, Pomegranate) extract as biomordants and alum (as a chemical mordant) with two mordanting procedures (pre- and metamordanting) on color characteristics of the dyed samples were also investigated. The CIEDE2000 values, color strength (K/S), washing fastness and tensile property of the mordanted and dyed samples were assessed. Visually, a range of hues from orange to brownish-red were obtained. In general, pre-biomordanted samples with Rhus coriaria (10 %owf), Eucalyptus (10 %owf), Terminalia chebula (5 %owf), Quercus castaneifolia (5 %owf) and Pomegranate (5 %owf) showed almost the same color difference (ΔE₀₀) and wash fastness values compared to those treated with 3 %owf alum. Finally, it was concluded from the comparative studies that the biomordants have good potential to be considered as alternatives to the common chemical mordants.     

Investigation on the Biodegradability and Antibacterial Properties of Nanohybrid Suture Based on Silver Incorporated PGA-PLGA Nanofibers

Polyglycolic acid-poly lactic glycolic acid (PGA-PLGA) electrospun nanofibers containing silver nanoparticles have been produced and twisted into the nanofibrous yarn. The morphology of nanofibers and produced yarns, as well as the mechanical properties of the yarns, were investigated. Furthermore, in vitro antibacterial properties and in vitro degradation behavior of yarns containing various silver nanoparticles were studied. SEM images confirmed that the addition of the silver nanoparticles into the polymer solution increases the fiber diameters. The result of the mechanical test of the yarns alone and used in two different forms of the knots was measured and results showed that the strength of the yarns without the knot was significantly more than that of others. The biodegradability test showed that the mechanical properties and the weight of the yarns were quickly reduced after subjecting to in vitro condition. The result of the antibacterial test indicated that the nanofiber yarns containing %3 silver nanoparticles were the most appropriate sample with a considerably antibacterial activity against both gram-positive bacterium Staphylococcus aureus and gram-negative bacterium Escherichia Coli with inhibition zones of 8.1 and 9.5 mm, respectively; which demonstrated that silver nanoparticles retained their effectiveness after the electrospinning process. Therefore the nanofibrous yarns containing silver nanoparticles could be successfully produced by the electrospinning process with the proper antibacterial property as a candidate for the surgical sutures.     

Comparative effectiveness of different infiltration models in estimation of watershed flood hydrograph

The present study aims to evaluate performance of different infiltration models, namely initial and constant rate, soil conservation service (SCS) curve number and Green–Ampt in simulation of flood hydrographs for the small-sized Amameh Watershed, Iran. To achieve the study purpose, the infiltration rates were measured using rainfall simulator in work units acquired through overlaying topography, land use, drainage network and soil hydrologic group maps. All parameters of the study infiltration models were determined with the help of the Infilt. software package. The performances of the models in simulation of the observed output hydrographs from the entire watershed were ultimately evaluated for 28 rainfall–runoff events in the HEC-HMS environment. The different components of the observed and estimated hydrographs including time to peak, runoff volume, peak discharge, discharge values and peak time deviation were compared using relative error (RE), coefficient of determination (R²), peak-weighted root mean square error (PWRMSE) and Nash–Sutcliffe (NS) criteria. The general performance of estimations was also qualitatively assessed using scatter plot and distribution of study variables around standard lines of 1:1 slope. The results revealed that the SCS infiltration model with PWRMSE = 0.61 m³ s^?1 and NS = 0.53 performed better than initial and constant rate model with PWRMSE = 1.1 m³ s^?1 and NS = 0.54, and Green Ampt model with PWRMSE = 1.35 m³ s^?1 and NS = 0.29 in estimation of flood hydrograph for the Amameh Watershed.     

The effect of silver nanoparticle on Fenugreek seed germination under salinity levels

A study was conducted to evaluate the effect s of silver nanoparticles for ameliorating negative effects of salinity on germination and growth of Fenugreek seeds. In order to investigate salinity stress on Fenugreek germination indices, an experiment was carried out in Iran from October 2014 to November 2014 at Ferdowsi University of Mashhad, in the biotechnology Laboratory, to create salinity stress, sodium chloride (NaCl) at the levels of 0 (as control), 5, 10, 15 and 20 dS/m and Five levels of silver nanoparticles (0, 10, 20, 30 and 40 μg mL^–1) on Fenugreek seed (Trigonella foenum-graecum); were tested Germination characteristics such as total germination (GT), Germination Speed Index (GSI), Shoot and Root of seedling long, Fresh Mass and Dry Mass were measured. Results showed the great effects of silver nanoparticles to improve salinity stress on Fenugreek seed germination. Results showed a significant reduction in germination percent and seedling growth due to the salinity stress while significantly increased with silicon nano-particles application. In without silver nanoparticle low level of salinity (0 dS/m) increased seed germination percentage, while the high levels (5, 10, 15 and 20 dS/m) inhibited the seed germination significantly. The results showed that the effect of AgNPs was significant on germination percentage in P ≤ 0.05. Overall, application of AgNPs was beneficial in improving salinity tolerance in the Fenugreek seedling and its application may stimulate the differences defense mechanisms of plants against salt toxicity.     

Effects of melatonin on oocyte maturation in PCOS mouse model

The purpose of oocyte in vitro maturation is generation of mature oocytes that could support future development. Efforts have been made to enhance oocyte developmental competence by developing optimal culture conditions. The present study is conducted to determine melatonin effects on quality of polycystic ovarian syndrome (PCOS) oocytes when it has been added during in vitro maturation, and immature oocytes were cultured in defined conditioned medium with and without different melatonin concentrations. Melatonin could significantly improve nuclear maturation of PCOS oocytes (81.1% vs. 56.3%, P < 0.05 were achieved with 10^?6 mol/L concentration). Cleavage rate was significantly higher in 10^?5 mol/L concentration compared to untreated oocytes in PCOS (54% vs. 35%, respectively) and it was significantly higher with 10^?6 mol/L concentration in the control group, 55% versus 38%, compared to untreated oocytes. This study showed that melatonin has the potential to induce oocyte nuclear maturation and guarantee fertilization potential. © 2016 Japanese Society of Animal Science     

Estimating Cape hare occupancy and abundance in southern Tunisia using spotlighting data

This study outlines the importance of integrating spotlighting data and occupancy modelling to estimate the spatial occupancy, abundance and habitat preferences of Cape hares Lepus capensis in southern Tunisia. Exploring the spatial distribution pattern of this species is problematic because of its nocturnal and secretive behaviour. In order to overcome these constraints, we used spotlight surveys on equidistant points along tracks to collect presence/absence data. Using a likelihood-based approach, we estimated occupancy and relative abundance of Cape hare populations in three national parks: Sidi Toui, Bouhedma and Jbil. We also combined the three parks in a single area to represent ‘southern Tunisia’ for investigating the factors affecting its distribution pattern. Sidi Toui showed the highest occupancy and relative abundance values (88.84 ± 10.95% and 7.97 hares km^?2, respectively). Bouhedma was also recognised as high-quality hare habitat with occupancy and relative abundance values of 61.70 ± 11.70% and 2.85 hares km^?2, respectively. The lowest occupancy (49.65 ± 26.25%) and relative abundance (2.10 hares km^?2) estimates were encountered in Jbil. The combined parks model suggested that part of the distribution pattern of Cape hares can be explained by habitat quality. Spatial occupancy over southern Tunisia showed a positive relationship between its occurrence and the availability of Poaceae and Plantaginaceae.