Expression of reproductive-related genes and changes in oocyte maturation of goldfish broodstock (Carassius auratus) following injection of different exogenous kisspeptins

Kisspeptin, upstream of the hypothalamic-pituitary-gonadal axis, play an essential role in the reproductive process. In the present study, the effect of different types of kisspeptin, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss) and their combination (Kiss1+H) on the reproductive-related genes (kiss1, Kissr and Cyp19) of adult female goldfish was investigated in comparison with Ovaprim (a synthetic GnRH hormone). Kiss1 and Hkiss were synthesized using a solid-phase synthesis approach. Peptides were injected at a dose of 100 μg/kg body weight. The brain and ovarian tissues of samples were separated for histological studies 24 hr post-injection. The expression of the kiss1, Kissr and Cyp19 genes was measured by RT-PCR. The results showed a significant increase in expression of the reproductive-related genes. Histological analysis revealed higher number of mature oocytes in kisspeptin treated groups compare to other ones. In conclusion, Hkiss and Kiss1+H are the most effective peptides in oocyte maturation and expression of reproductive-related genes. In addition, it seems that kisspeptins in other domestic animals can be used to stimulate the hypothalamus-pituitary-gonadal axis.     

Potential application of Fe-methionine as a feed supplement on improving the quality of broilers breast meat

A total of 250 one-day-old Ross 308 male chicks were used to investigate the effects of Fe-methionine (Fe-met) and iron sulfate (FeSO₄.7H₂O) on performance, mineral concentration, meat quality, and oxidative stability of breast. The feeding experiment was performed with five dietary groups including group I (basal diet [BD] + 80 mg/kg FeSO₄), group II (BD + 60 mg/kg FeSO₄ + 20 mg/kg Fe-met), group III (BD + 40 mg/kg FeSO₄ + 40 mg/kg Fe-met), group IV (BD + 20 mg/kg FeSO₄ + 60 mg/kg Fe-met), and group V (BD + 80 mg/kg Fe-met). The results showed that Fe and Cu content in the breast muscle increased by dietary supplementation of organic and inorganic iron from II to V groups. Dietary groups of II, IV, and V increased L* value and b* value of breast meat. Additionally, redness of breast meat enhanced linearly and quadratically by dietary supplementation of 80 mg/kg Fe as FeSO₄. In addition, dietary supplementation Fe from II to V groups led to a significant increase in pHu at 24-h postmortem while it was negatively correlated with L* value of breast muscle. This study demonstrates that replacing FeSO₄ with Fe-Met could improve breast meat yield of broilers. Although 80 mg of Fe-Met improved the iron content of meat, it showed detrimental effects on the lipid oxidation of breast meat.     

Comparison of different soilless media for growing gerbera under alkalinity stress condition

A factorial experiment was developed with two factors of the growing medium (v/v): 100% coconut fiber (CF), 75% vermicompost+25% perlite (VP), 25% zeolite+75% perlite (ZP), 75% peat+25% perlite (PP), 75% coco chip+25% perlite (CCP), 75% coconut fiber+25% perlite (CFP) and 100% perlite (P); and sodium bicarbonate (NaHCO₃) (0, 20 and 40 mM) involving the growth of gerbera cv. Dafne. Compared with CF substrate, NaHCO₃ in the nutrient solution caused significant decrease in vegetative and reproductive traits and nutrient concentration [especially iron, phosphorus, and magnesium (Fe, P and Mg)] in the rest of the media. The improved crop performance of plants grown onto CF substrate was attributed to their strong capacity to accumulate Fe in the aerial part under alkaline conditions and to maintain a better plant nutritional status (higher P and Mg). It is concluded that the use of CF substrate could provide a useful tool to improve alkalinity tolerance of gerbera plants under NaHCO₃ stress.     

Assessment of some soil thermal conductivity models via variations in temperature and bulk density at low moisture range

Simulation of heat transfer in soil under steady and unsteady situations requires reliable estimate of soil thermal conductivity (λ) at varying environmental conditions. In the current work several soil thermal conductivity predicting models including I) de Vries, II) Campbell, III) combined de Vries and Campbell and IV) de Vries-Nobre were evaluated for the four soils of coarse sand, sandy loam, loam and clay loam textured at varying in temperature and bulk density at low moisture range. Thermal conductivities measured by the cylindrical probe method served as the reference for models assessment. Results showed that approximately same thermal conductivities obtained by the five methods at low moisture range (θ ≤ 0.05 m³/m³). Also the de Vries and de Vries-Campbell models produced accurate than Campbell and de vries-Nobre models. The accuracy of the two models increased with soil compaction but decreased with temperature rise. Campbell model showed more reliability at higher (311.16 and 321.16 K) temperatures; but its accuracy declined with soil compaction in current work. It seems that assuming needle shape for the soil particles is far away from the reality whereas assuming spherical shapes may be more realistic and produced more satisfactory prediction of thermal conductivity. The compaction would alter particle arrangement and may increase the contact area of particles; and then make them behave more or less spherical shape.it seems thermal conductivity in solid particles increase via increasing in temperature. Since a modified mineral shape factor, g_m, was developed as a combination between sphere and needle according to geometric mean particle diameter as well as bulk density and temperature as modifying factors. This factor increased the accuracy of de Vries-Nobre model up to 10.37%. Regarding nonlinear regression model, moisture content, bulk density, temperature and quartz content demonstrated significant effect on soil thermal conductivity in our investigation.     

Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus

Susceptibility of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, and Pseudomonas aeroginosa to the essential oils extracted from two varieties of Thyme, i.e., Thymus kotschyanus Boiss. and Hohen. and Thymus persicus L. at preflowering and flowering stages were studied. The disk diffusion method was used to evaluate the zone of microbial growth inhibition at various concentrations of the oils. Minimal inhibitory concentration and minimal bactericidal concentration of the oils were determined and compared with each other. The oils from the above plants were found to be strongly bactericidal with that of T. kotschyanus being more effective. T. kotschyanusand T. persicus oils analyzed by gas chromatography (GC) and GC/mass spectrometry (MS) lead to identification of 33 and 26 components, respectively. The profile of the oil components from T. persicuswas similar to that of T. kotschyanus in almost all of the compounds but at different concentrations. The major components of T. kotschyanus oil before and at the flowering stages were carvacrol (35.06, 22.75%), thymol (26.60, 16.52%), gamma-terpinene (7.81, 0.34%), gamma-terpinene (4.34, 0%), borneol (2.29, 4.52%), myrcene (0.26, 12.65%), thymolquinone (0, 11.39%), nerol (0, 6.10%), and beta-caryophyllene (0, 5.54%), respectively, and those of T. persicus at the same stages were carvacrol (38.96, 27.07%), thymol (6.48, 11.86%), P-cymene (7.51, 10.16%), gamma-terpineol (0, 9.51%), nerol (15.66, 9.41%), gamma-terpinene (6.11, 6.51%), and thymol acetate (5.29, 5.30%), respectively. The contribution of oil components to its antibacterial property is discussed. High aromatic compound content of the phenol-rich oils seems to account for strong antibacterial activity.     

Assessment for salinity tolerance through intergeneric hybridisation: Triticum durum × Aegilops speltoides

Summary Triploid hybrid embryos (AABB × SS = ABS) were produced by crossing two moderately salt-tolerant wheats, namely Triticum turgidum durum and Aegilops speltoides subsp. speltoides. The embryos were rescued by culturing on agar No. 1. Chromosome doubling was achieved by using 0.05% colchicine. The resulting hexaploid plants grew to maturity, and produced a considerable amount of seed (≈40,000). The synthetic hexaploid (C₂) was tested for salinity tolerance, grown at 0, 100, 125, 150, 160, 170, 180 and 200 mM NaCl in a standard nutrient solution for 2 weeks. The criterion of salt tolerance was root growth in different NaCl concentrations. The amphidiploid material showed greater salinity tolerance than either parent, suggesting the presence of different genes for tolerance in the parents.     

Genome-wide identification and characterization of novel long non-coding RNA in Ruminal tissue affected with sub-acute Ruminal acidosis from Holstein cattle

Veterinary Research Communications - Sub-acute ruminal acidosis is a type of metabolic disorder in which affected cattle show a considerable depression of rumen pH. This leads to a dramatic decline...     

Prevalence,phenotypic and genotypic diversity,antibiotic resistance,and frequency of virulence genes in Pseudomonas aeruginosa isolated from shrimps

Preventive measures should be prioritized for the increased growth of bacterial contamination in aquatic organisms. This study is aimed at molecular investigation of the prevalence of Pseudomonas aeruginosa, antibiotic resistance, and virulence factor encoding genes. After confirmation of P. aeruginosa collected from shrimp samples (in the same size) in Isfahan and Chabahar (Iran) in summer and autumn, antibiotic resistance encoding genes, virulence factor encoding genes, and multi-drug resistance (MDR) patterns were assessed. Bacterial contamination from Isfahan was higher than Chabahar despite having a cooler climate on summer days. Antibiotic resistance to piperacillin in fresh shrimp samples in summer in Isfahan was contrary to its usage as a front-line antibiotic agent. Lowered MDR pattern in frozen shrimp viability of bacteria was related to the varied expression of antibiotic resistance, highlighting the importance of regulations for cold chain in storage, transportation, and distribution of marine samples compared to fresh shrimps. Foodborne pathogens are of clinical and environmental importance. Results of our study indicated a high rate of frequency for P. aeruginosa isolated from marine samples. Maintenance of cold chain has indispensable roles in preservation, and reduction of P. aeruginosa frequency in aquatic organisms.