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Physiology and Behaviour of the Pigeon, edited by M. Abs. 1983. 360 pages, illustrated. London, Academic Press Ltd. Price £34.00. ISBN 0 12 042950 0. 相似文献
2. A circadian system was postulated to control the restriction of ovulation to an 8‐h period of the day under conventional 14 h light: 10 h dark regimes.
3. The final phase of follicular maturation was postulated to commence after ovulation of the preceding ovum in the hierarchy.
4. Ovulation was postulated to occur when a mature follicle was present in the ovary during the appropriate phase of the circadian‐linked system.
5. The predicted times of oviposition were within the standard error of the observed times of oviposition under 21‐, 24‐ and 28‐h photoschedules.
6. It was concluded that this hypothesis for the control of the ovulatory cycle of the hen is consistent with current knowledge. 相似文献
2. In Experiment 1, nest‐site selection by members of a group of light hybrids was studied in a complex pen‐system. Eggs were clumped in distribution ; only one individual laid in the most secluded part of the system.
3. Experiment 2 provided light hybrids with an extensive outdoor area, but similarly clumped laying was observed; there was no indication that hens attempted to find concealment for nesting.
4. In Experiment 3, hens from a group of light hybrids just coming into lay and from a group of mature medium hybrids were tested singly, in an area that included a cage containing some of their flockmates. Most subjects from both groups laid near the other birds rather than in isolation.
5. In semi‐intensive conditions, the majority of hens are apparently gregarious rather than solitary in their nesting behaviour. 相似文献
2. A series of alternative cryoprotective compounds were tested for their effect on fertility when fowl spermatozoa were inseminated fresh in their presence. Under these circumstances dimethylsulphoxide, dimethylaceta‐mide, ethane‐diol, propane‐diol and methylpyrrolidone did not depress fertility when used in concentrations equivalent to that of glycerol or in amounts reported previously to be non‐toxic and adequate to protect cells during freezing.
3. Dimethylacetamide was compared with propane‐diol for use in freezing fowl semen and the former enabled encouraging levels of fertility to be obtained. 相似文献
- An enzymatic ‘reaction rate’ micro-method for the rapid routine estimation of D-B-hydroxybutyrate (D-B-HOB) in ruminant plasma, using an I.L. Multistat III centrifugal analyzer, is described.
- Reaction conditions were optimized to give a linear response for plasma D-B-HOB concentrations between 100 and 2500 μmoles per litre, at 30°C and pH 9.0.
- For the standardized method the within-run and between-run coefficients of variation for deproteinised ovine plasma were consistently less than 3.5%.
- There was good agreement between plasma concentrations obtained by the present method and both original U.V. end-point technique (r=0.927b=0.950) and a colorimetric end-point procedure (r=0.937. b=0.879).
- Utreated ovine and bovine plasma consistently exhibited high ‘blank’ activity and this was directly correlated with plasma lactate dehydrogenase (LDH) activity in both species (r=0.971; p<0.001 and r=0.949; p<0.001 respectively). The distribution of LDH activity in man was similar to sheep but, contrastingly, non-specific interference was extremely low in human plasma and unrelated to LDH. Horse, chicken and rat had negligible ‘blank” activity and comparatively low LDH levels. In both cattle and sheep non-specific interference was abolished by perchloric acid precipitation. In the sheep subtraction of ‘blank’ activity gave D-H-HOB concentrations for untreated plasma comparable to those in deproteinised samples. However, in the bovine, D-B-HOB levels remained significantly (t=6.44; p<0.001) higher even after ‘blank’ correlation. In contrast to man and other non-ruminants, perchloric acid precipitation is essential in ruminants to avoid false overestimation of plasma D-B-HOB levels.
- Plasma with EDTA as anticoagulant and serum gave concentrations of D-B-HOB approximately 60% lower, than samples containing heparin or oxalate/fluoride. However, heparin was associated with much higher (up to 50%) non-specific NAD rduction than oxalate/fluoride.
- High levels of acetoacetate (400–1000 μmoles per litre) reduced the recovery of D-B-HOB from ovine plasma by less than 10%. This effect was negated by the inclusion of hydrazine hydrates in the reaction mixture. Perchlorate ion concentrations above 25 μmoles per litre per test dramatically inhibited the assay in ovine plasma, and therefore precipitation conditions must be carefully controlled.
- Plasma with oxalate/fluoride as anticoagulant showed the greatest stability in storage; 24 hours at room temperature, one week at +4°C and at least one month at ?20°C.