IMPACT OF SULFUR FERTILIZATION ON DIFFERENT FORMS AND BALANCE OF SOIL SULFUR AND THE NUTRITION OF WHEAT IN WHEAT-SOYBEAN CROPPING SEQUENCE IN TARAI SOIL

A field experiment was conducted at farmer's field in Mollisols of Tarai soils in Uttarakhand (India), to assess the direct and residual effect of sulfur fertilization in wheat-soybean cropping sequence. Four levels of sulfur (S; 0, 15, 30, and 45 kg ha^?1) were applied to main crop (wheat) along with recommended dose of nitrogen (N), phosphorous (P), and potassium (K). The direct and residual effect of sulfur at highest level showed 27 and 6 percent increase in grain yield of wheat and soybean over control, respectively. The increase in grain yield of wheat was significant at each sulfur level. The direct as well as residual effect of sulfur showed significant increase in sulfur concentration and its uptake by grain and straw except increase in sulfur concentration and uptake by soybean straw. In wheat-soybean cropping sequence, the agronomic efficiency and apparent sulfur recovery decreased with increase in levels of sulfur, but the percent response increased with increasing sulfur application. Different forms of sulfur such as total sulfur, organic sulfur, calcium chloride extractable sulfur, potassium dihydrogen phosphate extractable sulfur, and non-sulfate sulfur in post-harvest soil increased according to sulfur level applied but it decreased under control and also after residual crops. The buildup of sulfur in surface soils was greater than in the deeper soils. Application of sulfur showed the positive sulfur balance and it increased with increase in sulfur level, while it was negative under control. A major portion (46–62%) of applied sulfur contributed to increase in sulfur content of root zone soil followed by unaccounted component (25–40%) and small portion (11–18%) was absorbed by wheat plant as uptake.     

Effect of Conditioning Zinc Sulfate Heptahydrate (ZnSHH) with Zinc Oxide (ZnO) and Neem Oil on Growth,Productivity, Zinc Biofortification of Grain and Zinc Uptake by Basmati Rice

Commercial grade zinc (Zn) sulfate hepta hydrate (ZnSHH) is the most widely used source of Zn in India and several other countries for amelioration of Zn deficiency in crops. However, it releases water of hydration at temperature above 30°C and forms lumps on storage, which make it difficult to handle it and apply in fields. Therefore, conditioning of ZnSHH with ZnO and neem oil reduces the release of water of hydration and prevents lumps formation and can be well stored. Field experiments were conducted at the research farm of the Indian Agricultural Research Institute, New Delhi, India during rice growing seasons (July-November) of 2009 and 2010 to study the effect of conditioning ZnSHH with ZnO and neem oil on growth, productivity and Zn fortification of rice (Oryza sativa) grain and uptake by Basmati rice ‘Pusa 1121’. The experiment was conducted in a randomized block design with 3 replications comprised of 9 treatments of Zn fertilization. The present study shows that when conditioned with 2% ZnO and 4% neem oil ZnSHH improved yield attributes, grain and straw yields, Zn uptake and partial factor productivity (PFP), agronomic efficiency (AE), recovery efficiency (RE), and physiological efficiency (PE) of Zn in Basmati rice ‘Pusa 1121’. In general, ZnO was inferior to ZnSHH. Application of ZnSHH conditioned with 2% ZnO and 4% neem oil can be a better source of Zn for transplanted puddled Basmati rice on Zn deficient soils.     

Molecular phylogeny in Indian Citrus L. (Rutaceae) inferred through PCR-RFLP and trnL-trnF sequence data of chloroplast DNA

The taxonomy and phylogeny of Indian Citrus is revisited using PCR-RFLP of the trnD-trnT and rbcL-ORF 106 regions as well as sequence data analysis of the trnL-trnF intergenic spacer region of cpDNA. The study was based on 50 accessions of Citrus genotypes, collected from wild, semi-wild and domesticated stocks. Of the 13 restriction enzymes (RE) used for restriction digestion of the polymerase chain reaction (PCR) amplicons, four (Hinf I, Msp I, Alu I, Hae III) generated 47 restriction fragments, of which 24 (51%) were polymorphic. PCR-RFLP data showed a genetic distance ranging from 0 to 0.79 among 50 accessions of Citrus, and a cluster analysis, based on Neighbor-Joining (NJ) method, placed all the accessions in eight major clusters. Analysis of trnL-trnF sequences from 23 representative accessions of Citrus showed a pair-wise sequence divergence rate in the range of 0–0.064. NJ, minimum evolution (ME) and maximum parsimony (MP) analyses of trnL-trnF sequences produced phylogenetic trees, which placed all the 23 accessions in five clusters. PCR-RFLP analysis resulted in a well resolved phylogenetic tree with branches supported by moderate to high bootstrap values, while the trnL-trnF sequence-based trees showed only moderate to low bootstrap support for the internal tree branches, indicating uncertain origin of some Citrus genotypes. This study shows that the trnL-trnF spacer sequence data can detect genetic variation in Indian Citrus genotypes, but the utility of the data in inferring phylogeny at intra and inter-specific levels is limited probably by factors such as hybridization, bud mutations, apomixis and polyploidy. However, PCR-RFLP and trnL-trnF data supported the recognition of C. maxima, C. medica, and C. reticulata as the basal species of edible Citrus.     

Multi-model comparison on the effects of climate change on tree species in the eastern U.S.: results from an enhanced niche model and process-based ecosystem and landscape models

Context

Species distribution models (SDM) establish statistical relationships between the current distribution of species and key attributes whereas process-based models simulate ecosystem and tree species dynamics based on representations of physical and biological processes. TreeAtlas, which uses DISTRIB SDM, and Linkages and LANDIS PRO, process-based ecosystem and landscape models, respectively, were used concurrently on four regional climate change assessments in the eastern Unites States.

Objectives

We compared predictions for 30 species from TreeAtlas, Linkages, and LANDIS PRO, using two climate change scenarios on four regions, to derive a more robust assessment of species change in response to climate change.

Methods

We calculated the ratio of future importance or biomass to current for each species, then compared agreement among models by species, region, and climate scenario using change classes, an ordinal agreement score, spearman rank correlations, and model averaged change ratios.

Results

Comparisons indicated high agreement for many species, especially northern species modeled to lose habitat. TreeAtlas and Linkages agreed the most but each also agreed with many species outputs from LANDIS PRO, particularly when succession within LANDIS PRO was simulated to 2300. A geographic analysis showed that a simple difference (in latitude degrees) of the weighted mean center of a species distribution versus the geographic center of the region of interest provides an initial estimate for the species’ potential to gain, lose, or remain stable under climate change.

Conclusions

This analysis of multiple models provides a useful approach to compare among disparate models and a more consistent interpretation of the future for use in vulnerability assessments and adaptation planning.

     

Growth and Biochmical Studies of Mrigal Fish (Cirrhinus mrigala) Fed Graded Levels of Oil

A 45-day experiment was carried out to evaluate the growth performance,digestive enzymes activity and biochemical response of Cirrhinus mrigala fed graded levels of oil.Three isonitrogenous purified diets(6%,8%and 10%of oil)were formulated.A total of 108 juveniles of Cirrhinus mrigala(5.95±0.25)g were equally distributed in triplicate groups in nine plastic tanks of 100 L capacity and fed under natural light cycle.Growth performance of the fish significantly reflected the effect of inclusion levels of oil tested but the diet with 8%lipid had the best significant growth performance[%weight gain,Feed Conversion Ratio(FCR)and Specific Growth Rate(SGR)],across different experimental groups.Levels of secretion of digestive enzymes in the fish fed different experimental diets resulted in significant values of(p0.05)amylase and lipase,while Aspartate transaminase(AST),Alanine transaminase(ALT),Lactate dehydrogenase(LDH)and Malate dehydrogenase(MDH)values in the muscle and liver decreased significantly(p0.05)as the levels of oil in the diet increased.However,serum biochemical parameters did not record any significant difference(p0.05)with the exception of the total lipid across diets.The study confirmed the inclusion effect of graded levels of oil in juvenile Mrigal diet,but the inclusion should not be more than 8%to avert growth depression.     

Colonization by Rhizobia of the seed and roots of legumes in relation to exudation of phenolics

A study of the colonization pattern of Rhizobium inoculated either in the soil or on to the seeds of green gram (Phaseolus aureus Roxb.), black gram (P. mungo L.) and sun hemp (Crotalaria juncea L.) revealed a significant reduction of Rhizobium in the spermosphere of sun hemp which was attributed to excretion of phenolic compounds by the seed. As the plants aged, the quantity of phenolics exuded by the roots was reduced and there was a parallel increase in the Rhizobium population in the rhizospheres of the three plant species.     

Influence of moisture regime on inhibition of nitrification by DCD

A laboratory study at the Indian Agricultural Research Institute, New Delhi showed that DCD was an efficient nitrification inhibitor under field capacity moisture (???30 kPa) but not so under submerged soil conditions. Nitrification inhibition by DCD was 79.6% after 1 week of incubation and decreased to 59.3% after 3 weeks of incubation under field capacity moisture, while it was only 19.3% after 1 week of incubation and 10.6% and 18.4% after 2 and 3 weeks of incubation under submergence, respectively. DCD can play an important role in reducing N losses from agricultural fields.     

Temporal variation in mineral nitrogen in soil as influenced by incorporation of legume or cereal residues under submergence or well drained conditions

A laboratory study was conducted at the Indian Agricultural Research Institute, New Delhi on a sandy clay loam soil of pH 7.9 and organic C content of 0.34% to study the effect of incorporating Sesbania or Vigna legume residues or wheat straw at 15 and 30t ha^?1 on temporal variation in ammoniacal and nitrate‐N in soil under submergence and well drained conditions. Under submergence most mineral N was present as ammoniacal‐N, while under well drained conditions it was present as Nitrate‐N. The content of ammoniacal N in soil was the highest after 30 days of incubation and declined thereafter under submergence. On the other hand under well drained conditions the mineral‐N (mostly nitrate) content in soil at 30 DAI was very little and showed increases only later, reaching the highest level at 90 DAI. Application of wheat straw specially at 301 ha^?1 level resulted in immobilization of native soil‐N. These results show that rice which is grown under submergence can be transplanted soon after incorporation of legume residues, but for wheat or other crops which are grown under well drained condition a time interval of 30 days or more needs to be provided before sowing the crop.     

Non-suture intestinal anastomosis by invagination

Intestinal anastomoses were performed on seventeen mongrel dogs by non-suture invagination technique and by the conventional method. A comparative evaluation revealed the non-suture technique to be easier and less time-consuming. Also the tissue reaction was minimal. Encouraging results suggest further investigation prior to clinical trial of this relatively new technique. Résumé. Des anastomoses intestinales sur dix-sept chiens métis par l'invagination technique pas de suture et la méthode de convention. Une évaluation comparative a révélé que la technique pas de suture était plus facile et moins de temps était perdu. Aussi la réaction du tissu était minimum. Les résultats encourageants proposent un surcro***ît d'investigation antérieurement a l'expérience clinique de cette technique relativement plus nouvelle. Zusammenfassung. Die Darmanastomosen von siebzehn Mischhunden durch die Intussuscep-tiontecknik keiner Naht und die verabredete Methode. Eine vergleichende Abschatzung zeigte, daß die Tecknik keiner Naht leichter ist, und wenigere Zeitverschwendung hat. Auch war die Reaktion des Gewebes minderst. Die ermutigenden Ergebnisse suggerieren eine Forderung der Erforschung vor klinischem Versuch dieser Tecknik, die verhältnismäßig neuere ist.